FastQCFastQC Report
Mon 6 Jun 2016
ERR1211257_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211257_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6360619
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190450.299420543818141No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158360.24896947922835813No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98580.15498491577627901No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105250.048.3793831
GTATCAA186050.039.8525431
GTACTAG40250.033.291471
ATCAACG221250.032.2940143
CAACGCA223550.031.9279215
TACCTGG43250.030.6452562
TATCAAC241200.030.5540662
AACGCAG249850.029.3381566
TCAACGC250200.028.7640234
GTGGTAT40550.026.6680321
ACGCAGA273750.026.124717
TAGGCAT55650.026.0059725
GTACATG252000.025.9339241
CGCAGAG278900.025.608218
TACATGG253650.025.2187462
CTGTGCG55600.024.507979
GCATCGT51200.024.4115758
ACATGGG252250.024.393283
CTAGGCA61000.024.3511244
GGGCACC51050.024.12898494
Produced by FastQC (version 0.11.5)