##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211257_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6360619 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14164486192303 33.0 31.0 34.0 30.0 34.0 2 32.28104780368074 34.0 31.0 34.0 30.0 34.0 3 32.357872087606566 34.0 31.0 34.0 30.0 34.0 4 35.97611301667338 37.0 35.0 37.0 35.0 37.0 5 35.86193749381939 37.0 35.0 37.0 35.0 37.0 6 35.887932605301465 37.0 35.0 37.0 35.0 37.0 7 35.82316972609112 37.0 35.0 37.0 35.0 37.0 8 35.811333613913995 37.0 35.0 37.0 35.0 37.0 9 37.41539982193557 39.0 37.0 39.0 34.0 39.0 10-11 37.39271185713214 39.0 37.0 39.0 34.0 39.0 12-13 37.29231510329419 39.0 37.0 39.0 34.0 39.0 14-15 38.51205370106274 40.0 38.0 41.0 34.0 41.0 16-17 38.46598970949211 40.0 38.0 41.0 34.0 41.0 18-19 38.48748840325132 40.0 38.0 41.0 34.0 41.0 20-21 38.447529713696106 40.0 38.0 41.0 34.0 41.0 22-23 38.31798713301332 40.0 38.0 41.0 34.0 41.0 24-25 38.255629287023794 40.0 38.0 41.0 34.0 41.0 26-27 38.11324070817636 40.0 37.5 41.0 33.0 41.0 28-29 38.09172086867646 40.0 37.5 41.0 33.0 41.0 30-31 38.10467974893639 40.0 37.5 41.0 33.5 41.0 32-33 37.97291089121987 40.0 37.0 41.0 33.0 41.0 34-35 37.83326960787936 40.0 37.0 41.0 33.0 41.0 36-37 37.689431248751106 40.0 37.0 41.0 33.0 41.0 38-39 37.59886883650789 40.0 37.0 41.0 33.0 41.0 40-41 37.4950243050244 40.0 36.0 41.0 32.0 41.0 42-43 37.34923935862217 40.0 36.0 41.0 32.0 41.0 44-45 37.20138723919794 39.5 36.0 41.0 31.0 41.0 46-47 36.963887319771864 39.0 36.0 41.0 31.0 41.0 48-49 36.72547208062612 39.0 35.0 41.0 30.5 41.0 50-51 36.52312825214024 39.0 35.0 40.5 30.0 41.0 52-53 36.31321668535719 39.0 35.0 40.0 30.0 41.0 54-55 36.070003328292415 38.0 35.0 40.0 29.0 41.0 56-57 35.81114463859571 38.0 35.0 40.0 28.5 41.0 58-59 35.535248298947 38.0 34.0 40.0 28.0 41.0 60-61 35.27080359631665 37.0 34.0 40.0 28.0 41.0 62-63 34.96006552192483 37.0 34.0 40.0 27.5 41.0 64-65 34.58412860446444 36.0 33.5 39.0 26.5 41.0 66-67 34.27636500472674 36.0 33.0 39.0 26.0 40.0 68-69 33.946786153989095 35.0 33.0 38.5 26.0 40.0 70-71 33.62972998382705 35.0 33.0 38.0 26.0 40.0 72-73 33.29384883766816 35.0 32.0 37.0 26.0 39.5 74-75 32.96298418440092 35.0 32.0 37.0 25.0 39.0 76-77 31.976069074409267 34.0 30.5 35.5 24.5 38.0 78-79 32.47628627025137 35.0 32.0 36.0 25.0 38.0 80-81 32.41049778331323 35.0 32.0 35.5 25.0 37.0 82-83 32.16699994135791 34.5 32.0 35.0 25.0 37.0 84-85 31.917276133030448 34.0 31.5 35.0 25.0 36.5 86-87 31.686597719498685 34.0 31.0 35.0 24.0 36.0 88-89 31.477002238304166 34.0 31.0 35.0 24.0 36.0 90-91 31.266770262454017 34.0 31.0 35.0 23.5 35.0 92-93 31.08695568780334 34.0 31.0 35.0 23.0 35.0 94-95 30.901961821640313 34.0 30.5 35.0 21.5 35.0 96-97 30.73532725352674 34.0 30.0 35.0 20.0 35.0 98-99 30.561497237926055 34.0 30.0 35.0 20.0 35.0 100 28.76753881972808 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.36405073495659934 1101 2 -0.3440751470778096 1101 3 -0.44053356485103023 1101 4 -0.23352083874488727 1101 5 -0.24585979316243112 1101 6 -0.2902470805896229 1101 7 -0.3122260843867153 1101 8 -0.2790598617341118 1101 9 -0.46559643850486765 1101 10-11 -0.466226133226435 1101 12-13 -0.4454370307347233 1101 14-15 -0.6607408854233157 1101 16-17 -0.6060629284741594 1101 18-19 -0.749560742804718 1101 20-21 -0.7710858562299947 1101 22-23 -0.7522294095012612 1101 24-25 -0.7486658024030319 1101 26-27 -0.7790532383577471 1101 28-29 -0.7804801158976886 1101 30-31 -0.718962461942553 1101 32-33 -0.6758951696179949 1101 34-35 -0.7594593575897974 1101 36-37 -0.6609049938297673 1101 38-39 -0.7006959071112959 1101 40-41 -0.794963020786156 1101 42-43 -0.8092068750392585 1101 44-45 -0.8154163879313145 1101 46-47 -0.7626214469563593 1101 48-49 -0.740928857439819 1101 50-51 -0.9386058710157954 1101 52-53 -0.8762151630598183 1101 54-55 -0.7780980338610917 1101 56-57 -0.7288104213118487 1101 58-59 -0.8374705117206389 1101 60-61 -0.8565044596759392 1101 62-63 -0.9609305476219205 1101 64-65 -0.8680360605519652 1101 66-67 -0.9358157948110986 1101 68-69 -0.9195756290875536 1101 70-71 -0.9890290309363436 1101 72-73 -1.1089526582071585 1101 74-75 -1.081427229498388 1101 76-77 -1.044574147232435 1101 78-79 -1.0075520280273835 1101 80-81 -0.8528772403716047 1101 82-83 -0.8846222171806843 1101 84-85 -0.8285349720641264 1101 86-87 -1.002661483414336 1101 88-89 -0.9027659881264611 1101 90-91 -0.893848730313394 1101 92-93 -1.0126228066046643 1101 94-95 -0.9731948200114786 1101 96-97 -1.1300360001718417 1101 98-99 -1.1762058551698509 1101 100 -1.0496417176978525 1102 1 -0.38244677080108147 1102 2 -0.34471575946401245 1102 3 -0.3263732090868352 1102 4 -0.22082170784442212 1102 5 -0.26568331029734793 1102 6 -0.37206123878026176 1102 7 -0.39084690162336244 1102 8 -0.3732599586198475 1102 9 -0.47074793772254964 1102 10-11 -0.45440847401118134 1102 12-13 -0.5099881034960916 1102 14-15 -0.5628267714336701 1102 16-17 -0.6529198199325279 1102 18-19 -0.6003553663974444 1102 20-21 -0.6708443194083316 1102 22-23 -0.6440119288574166 1102 24-25 -0.7576622426106994 1102 26-27 -0.8327099090615704 1102 28-29 -0.7649675575000217 1102 30-31 -0.7056659770036617 1102 32-33 -0.6944866412870141 1102 34-35 -0.770468820666359 1102 36-37 -0.7136475981178094 1102 38-39 -0.8085075516929336 1102 40-41 -0.7605422783421147 1102 42-43 -0.7954282590972426 1102 44-45 -0.7620489310228535 1102 46-47 -0.6601852228253904 1102 48-49 -0.7377210233928011 1102 50-51 -0.702013697963551 1102 52-53 -0.8579854442950534 1102 54-55 -0.8109213546617298 1102 56-57 -0.8901318909291618 1102 58-59 -0.8489011729488638 1102 60-61 -0.9227148590298739 1102 62-63 -0.9641171280782501 1102 64-65 -0.8224415999770827 1102 66-67 -1.001023519570765 1102 68-69 -1.0848990334738673 1102 70-71 -1.1044808774138346 1102 72-73 -1.033174195738745 1102 74-75 -1.1133435684134767 1102 76-77 -1.0002177119971911 1102 78-79 -0.7472699517857606 1102 80-81 -0.7104389011916936 1102 82-83 -0.7124460542241202 1102 84-85 -0.686779133844162 1102 86-87 -0.7970888744175149 1102 88-89 -0.9827701003943226 1102 90-91 -1.0311586502316885 1102 92-93 -1.0394841343634589 1102 94-95 -1.0402301620963428 1102 96-97 -1.1598570855896249 1102 98-99 -1.1159965242396623 1102 100 -1.054806207675746 1103 1 -0.34271844580784006 1103 2 -0.2969144536238062 1103 3 -0.35398539550005026 1103 4 -0.1713624742754689 1103 5 -0.22476237030193857 1103 6 -0.3197937284608159 1103 7 -0.3945173746094426 1103 8 -0.2922162805985167 1103 9 -0.4914029651716305 1103 10-11 -0.47206830192316573 1103 12-13 -0.5383363132639403 1103 14-15 -0.6608920610627891 1103 16-17 -0.6636730592329769 1103 18-19 -0.7040242469680749 1103 20-21 -0.6705124421677837 1103 22-23 -0.6674106732601501 1103 24-25 -0.7288891406806286 1103 26-27 -0.7166342666484269 1103 28-29 -0.8515213948343359 1103 30-31 -0.7285288066336619 1103 32-33 -0.6395108192136902 1103 34-35 -0.7850713150735587 1103 36-37 -0.7598020338431581 1103 38-39 -0.7400986336901312 1103 40-41 -0.8645004725251226 1103 42-43 -0.7106875281458045 1103 44-45 -0.8151907964884586 1103 46-47 -0.849863319760928 1103 48-49 -0.8447112956690503 1103 50-51 -0.9514166463430982 1103 52-53 -1.005643908980339 1103 54-55 -0.9778356642558705 1103 56-57 -0.987261614152473 1103 58-59 -1.0674311591737222 1103 60-61 -1.0750502032714095 1103 62-63 -0.9939345569017419 1103 64-65 -0.998512343108267 1103 66-67 -1.0816106059445687 1103 68-69 -1.0418951842931747 1103 70-71 -1.1553147971132347 1103 72-73 -0.9698315251955165 1103 74-75 -1.0606450790353357 1103 76-77 -1.0349528769357157 1103 78-79 -0.8094630918385519 1103 80-81 -0.805200628215367 1103 82-83 -0.8656384751599475 1103 84-85 -0.9417015281464707 1103 86-87 -1.016893581776202 1103 88-89 -1.063180473299326 1103 90-91 -0.9671149448267613 1103 92-93 -1.1059864101781507 1103 94-95 -1.0826607417117557 1103 96-97 -1.0434795688290812 1103 98-99 -1.098850287519216 1103 100 -1.1954929881867251 1104 1 -0.38449777778102856 1104 2 -0.3337285878787988 1104 3 -0.44681517454110065 1104 4 -0.2771933275598215 1104 5 -0.28607720738131803 1104 6 -0.41904931363154674 1104 7 -0.394343433812395 1104 8 -0.36168162095287215 1104 9 -0.5201385026651693 1104 10-11 -0.4760658804036453 1104 12-13 -0.607569575640099 1104 14-15 -0.7368391086825525 1104 16-17 -0.6711901732185765 1104 18-19 -0.7094068202185682 1104 20-21 -0.7628993665273143 1104 22-23 -0.7016897204317658 1104 24-25 -0.7452093301466434 1104 26-27 -0.8123138855886296 1104 28-29 -0.892466034586235 1104 30-31 -0.8253789953558979 1104 32-33 -0.7799711315256914 1104 34-35 -0.8028081570053942 1104 36-37 -0.7455276494146901 1104 38-39 -0.7854990647055331 1104 40-41 -0.7949320975511398 1104 42-43 -0.6675604492119902 1104 44-45 -0.825838668291027 1104 46-47 -0.8364482828967752 1104 48-49 -0.7842217358828449 1104 50-51 -0.787344459264709 1104 52-53 -0.7756381564592587 1104 54-55 -0.8947923499375534 1104 56-57 -0.880286140981724 1104 58-59 -0.881184639166527 1104 60-61 -0.8686835699649649 1104 62-63 -0.9509095122514424 1104 64-65 -0.9405272718890458 1104 66-67 -0.8862673235428531 1104 68-69 -0.9673357793919521 1104 70-71 -0.9826857349248144 1104 72-73 -0.89015210585179 1104 74-75 -1.0235500857058142 1104 76-77 -0.9987172900138042 1104 78-79 -0.807699440428145 1104 80-81 -0.7489350170876747 1104 82-83 -0.7016552591523109 1104 84-85 -0.666694596405641 1104 86-87 -0.8426173779128803 1104 88-89 -0.9304604676127077 1104 90-91 -0.7892564784997234 1104 92-93 -0.8791540768670991 1104 94-95 -0.8808962429578244 1104 96-97 -0.9225549969964781 1104 98-99 -1.0474687880412752 1104 100 -1.2241901598481668 1105 1 -0.3792192638369549 1105 2 -0.3389867084874396 1105 3 -0.4849518220427065 1105 4 -0.32126697753562183 1105 5 -0.3532465726881284 1105 6 -0.4112834555343383 1105 7 -0.383302721585963 1105 8 -0.48064652302922184 1105 9 -0.5575080919597539 1105 10-11 -0.6191405695630579 1105 12-13 -0.6140035999276847 1105 14-15 -0.8177095085099637 1105 16-17 -0.7782875564790004 1105 18-19 -0.7369310593630871 1105 20-21 -0.8117240939315238 1105 22-23 -0.833254606824525 1105 24-25 -0.8683677798184064 1105 26-27 -0.954279840522041 1105 28-29 -0.8928471240858613 1105 30-31 -0.8395119712390553 1105 32-33 -0.8387661693889257 1105 34-35 -0.8660294689875414 1105 36-37 -0.8150225561645641 1105 38-39 -0.9192529915912786 1105 40-41 -0.9728526130946591 1105 42-43 -0.8742898087315325 1105 44-45 -0.9894407980564495 1105 46-47 -0.8726468331117729 1105 48-49 -0.9545095927793668 1105 50-51 -1.0109395308324594 1105 52-53 -1.004179459157477 1105 54-55 -0.9355820155300947 1105 56-57 -0.931387194107586 1105 58-59 -1.0261515406078203 1105 60-61 -0.9324869392945843 1105 62-63 -0.9298226719363996 1105 64-65 -0.9439208193208444 1105 66-67 -1.004919795629995 1105 68-69 -1.0114000507707956 1105 70-71 -0.9545573797390574 1105 72-73 -0.9949275997707829 1105 74-75 -1.0120183557210538 1105 76-77 -0.9029867370458824 1105 78-79 -0.8285158645309885 1105 80-81 -0.8914897784523461 1105 82-83 -0.8098890011353603 1105 84-85 -0.9393805897603364 1105 86-87 -0.9404451429307024 1105 88-89 -0.934638632556112 1105 90-91 -0.9089191072859748 1105 92-93 -1.0421967192730577 1105 94-95 -1.0555521302566575 1105 96-97 -1.0413303549988484 1105 98-99 -1.093759818818615 1105 100 -1.0439566660911588 1106 1 -0.48446019302140186 1106 2 -0.436525726077889 1106 3 -0.49754084951543476 1106 4 -0.3385760095180075 1106 5 -0.3777351301264176 1106 6 -0.4241739098128079 1106 7 -0.46591654521935766 1106 8 -0.4539029625148956 1106 9 -0.6157005158731863 1106 10-11 -0.6022677582783373 1106 12-13 -0.6553242564587549 1106 14-15 -0.7878120579978898 1106 16-17 -0.7585068681854636 1106 18-19 -0.7778314147074497 1106 20-21 -0.7915297158328727 1106 22-23 -0.6939843746049661 1106 24-25 -0.7493106289480735 1106 26-27 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1.1757586860497433 2310 78-79 1.0219445457139074 2310 80-81 0.947465953435028 2310 82-83 1.0008263860703153 2310 84-85 1.068024955194204 2310 86-87 1.0644997730430674 2310 88-89 1.024693486217469 2310 90-91 1.1223525828228418 2310 92-93 1.1999581514856956 2310 94-95 1.265660944392721 2310 96-97 1.2305867786401414 2310 98-99 1.2167960293441453 2310 100 1.449920260439491 2311 1 0.3913727080651057 2311 2 0.33199928532020806 2311 3 0.40372523447150144 2311 4 0.2853598235785526 2311 5 0.3824267999960611 2311 6 0.4278333122882785 2311 7 0.4923880863741914 2311 8 0.46137184184523505 2311 9 0.6234717700021974 2311 10-11 0.653786760160159 2311 12-13 0.7187790034531929 2311 14-15 0.874000022557496 2311 16-17 0.6733277731763181 2311 18-19 0.7701954060703571 2311 20-21 0.7091889289274107 2311 22-23 0.8676915344348828 2311 24-25 0.9230113817209897 2311 26-27 0.3320653419409183 2311 28-29 0.6682755163187863 2311 30-31 0.8842703502233888 2311 32-33 0.9425583120204379 2311 34-35 0.9950091776561365 2311 36-37 1.032329118224915 2311 38-39 1.0344634140698403 2311 40-41 1.0217495626257431 2311 42-43 1.011808778945408 2311 44-45 1.04663411846483 2311 46-47 0.7641298063835436 2311 48-49 0.9975223177316863 2311 50-51 1.1157301719841684 2311 52-53 0.9962649439471463 2311 54-55 1.0426472287129087 2311 56-57 1.1700278119335792 2311 58-59 1.1569787450633626 2311 60-61 1.1557144149405474 2311 62-63 1.157099645949181 2311 64-65 1.2206319381993964 2311 66-67 1.2771266269387098 2311 68-69 1.2917885089942374 2311 70-71 1.3040016577384392 2311 72-73 1.28156101203178 2311 74-75 1.2979309078585857 2311 76-77 1.2297516039774088 2311 78-79 0.9746282412348961 2311 80-81 0.92212671761569 2311 82-83 0.9989905945064024 2311 84-85 1.0977510588930528 2311 86-87 1.1943533449352088 2311 88-89 1.1737739599648442 2311 90-91 1.1702293046659165 2311 92-93 1.2713125944044208 2311 94-95 1.231933319528494 2311 96-97 1.2675557427675095 2311 98-99 1.3288868347192349 2311 100 1.4353562769901345 2312 1 0.25002144994258657 2312 2 0.3115048202829769 2312 3 0.42478832004047007 2312 4 0.22039543715205667 2312 5 0.36252540306387004 2312 6 0.44939237263936604 2312 7 0.29942355111774077 2312 8 0.39985375905824583 2312 9 0.5830120548314355 2312 10-11 0.6142713593148486 2312 12-13 0.7121137693285391 2312 14-15 0.8799657397683447 2312 16-17 0.8521534350648778 2312 18-19 0.7996390768777673 2312 20-21 0.8320007236806006 2312 22-23 0.9271616236331397 2312 24-25 0.902988470699114 2312 26-27 0.9108198753305672 2312 28-29 0.9662208800670555 2312 30-31 0.973914240222598 2312 32-33 0.8332597525722534 2312 34-35 0.939709571036893 2312 36-37 0.9800048509739128 2312 38-39 0.7713346651068562 2312 40-41 0.8782560590858282 2312 42-43 0.9531926660001915 2312 44-45 0.7446532431151738 2312 46-47 0.7691034399974086 2312 48-49 0.9972269899739032 2312 50-51 0.8142302061357185 2312 52-53 0.9614312801962868 2312 54-55 0.8619683228190169 2312 56-57 0.9452994185755514 2312 58-59 1.0189867229070018 2312 60-61 1.0999897100565335 2312 62-63 1.134809034857426 2312 64-65 1.0860941544212395 2312 66-67 1.0840706009282002 2312 68-69 1.1463176739305396 2312 70-71 1.1043154873768088 2312 72-73 1.0883228623227765 2312 74-75 1.1304648162674624 2312 76-77 1.1001153441944886 2312 78-79 0.9754824051337891 2312 80-81 0.910798484174812 2312 82-83 0.9128426002635948 2312 84-85 0.9350968233885055 2312 86-87 0.9944502827801429 2312 88-89 0.9535028792673721 2312 90-91 1.1240906487527482 2312 92-93 1.1346150337253817 2312 94-95 1.1070162723964714 2312 96-97 0.7920605748625356 2312 98-99 0.5825481000805937 2312 100 0.7831652543749499 2313 1 0.05873970616330837 2313 2 0.22929186399837675 2313 3 0.2991174524044382 2313 4 0.22766127898904642 2313 5 0.3648191494095343 2313 6 0.24089213150685396 2313 7 0.23808118278391532 2313 8 0.36596678120972115 2313 9 0.5753300282371541 2313 10-11 0.35294568887399436 2313 12-13 0.4317700167945162 2313 14-15 0.4617450487494281 2313 16-17 0.7429119426324604 2313 18-19 0.670532711121723 2313 20-21 0.6641614168049799 2313 22-23 0.6481533113188789 2313 24-25 0.6184370625279243 2313 26-27 0.7932245817535133 2313 28-29 0.5789800975173591 2313 30-31 0.8106640798232476 2313 32-33 0.9172327450196107 2313 34-35 0.9574762536368056 2313 36-37 1.0006288827095133 2313 38-39 0.9907370832605054 2313 40-41 1.022261124291191 2313 42-43 1.0499174961049107 2313 44-45 0.9266727776938595 2313 46-47 0.9054765011568691 2313 48-49 1.0251913905639967 2313 50-51 1.011377160397359 2313 52-53 1.0480589886692115 2313 54-55 1.0260149140304122 2313 56-57 0.7089577615470688 2313 58-59 0.7766771606869938 2313 60-61 0.7060420116272397 2313 62-63 1.043259200093658 2313 64-65 1.0965520145586893 2313 66-67 1.0670043051361375 2313 68-69 1.192055816826091 2313 70-71 1.1327184077120336 2313 72-73 1.1771977622065535 2313 74-75 1.2451004750002284 2313 76-77 1.0976332441934993 2313 78-79 0.9813993630799942 2313 80-81 0.8595658809252882 2313 82-83 1.002027238059803 2313 84-85 0.9675774794396546 2313 86-87 1.0434456173283877 2313 88-89 1.0180298861087032 2313 90-91 0.8850007746111146 2313 92-93 1.1092526770000575 2313 94-95 1.1921558233920848 2313 96-97 1.2469771529716915 2313 98-99 1.2781737144705865 2313 100 1.2729189008817983 2314 1 -0.3128939389780676 2314 2 0.07249523938086355 2314 3 0.2972152098220491 2314 4 0.22129076543845372 2314 5 0.32213057757672203 2314 6 0.38176859625264825 2314 7 0.44151022070276724 2314 8 0.45756522740188643 2314 9 0.3999289235230066 2314 10-11 0.5740670029446377 2314 12-13 0.4529454350393962 2314 14-15 0.734002550479282 2314 16-17 0.7970017571455017 2314 18-19 0.8337679700439367 2314 20-21 0.8615175801230279 2314 22-23 0.967565702503407 2314 24-25 0.8324537655749324 2314 26-27 0.7507425279030926 2314 28-29 0.756488933329976 2314 30-31 0.8528442360768764 2314 32-33 0.914684481526912 2314 34-35 0.9117546716585494 2314 36-37 0.8363568984123049 2314 38-39 0.5892708416607704 2314 40-41 0.6651197340435644 2314 42-43 0.7935672023286529 2314 44-45 0.8983826121415959 2314 46-47 0.8955849136388565 2314 48-49 0.9316359201031759 2314 50-51 0.8764151468245913 2314 52-53 0.8338807737954781 2314 54-55 0.8195609609149486 2314 56-57 0.7735401783327518 2314 58-59 0.8933487165854288 2314 60-61 1.0136539471717398 2314 62-63 0.8978825069295269 2314 64-65 0.9737202993938965 2314 66-67 0.6856232065600807 2314 68-69 0.6371361292770388 2314 70-71 0.8158018300311483 2314 72-73 0.9662405450669382 2314 74-75 1.013583486870246 2314 76-77 0.8745232563640748 2314 78-79 0.7620240150851885 2314 80-81 0.8863423966340562 2314 82-83 0.8732598488778933 2314 84-85 0.9182169979943282 2314 86-87 0.9133333491870843 2314 88-89 0.8522967283536467 2314 90-91 1.0348275339074604 2314 92-93 0.8979430920175275 2314 94-95 0.8959490108239407 2314 96-97 0.9275908591982542 2314 98-99 1.074223872181303 2314 100 1.1049916512427274 2315 1 0.4586633869537522 2315 2 0.46761971396622215 2315 3 0.47379067251520013 2315 4 0.3345756384080829 2315 5 0.4062151295812413 2315 6 0.45149171845230995 2315 7 0.3995118302829468 2315 8 0.2791729963438101 2315 9 0.5012122482058388 2315 10-11 0.5907867982984598 2315 12-13 0.39630179267008714 2315 14-15 0.6635648297367354 2315 16-17 0.790663972737434 2315 18-19 0.7948209583833616 2315 20-21 0.5167153721416184 2315 22-23 0.8778098153881899 2315 24-25 0.8982259966974411 2315 26-27 0.8218706261369277 2315 28-29 0.8198154411847582 2315 30-31 0.8915768414728547 2315 32-33 0.8324228134444454 2315 34-35 0.8969225334515087 2315 36-37 0.8794843412651332 2315 38-39 0.8542392146695121 2315 40-41 0.8970211002021813 2315 42-43 0.9956584747964214 2315 44-45 1.045682480836284 2315 46-47 0.9966064838634452 2315 48-49 0.9215346821369721 2315 50-51 1.0188152914480426 2315 52-53 1.0469465513324892 2315 54-55 0.8561051271153204 2315 56-57 0.814940613694958 2315 58-59 0.8759632471457053 2315 60-61 0.910372549388974 2315 62-63 1.0264239033165623 2315 64-65 1.083811972973301 2315 66-67 1.1122106032030743 2315 68-69 1.1142024196939602 2315 70-71 0.9816020686825766 2315 72-73 1.0604436136398832 2315 74-75 0.4131610345204706 2315 76-77 0.4251163129465745 2315 78-79 0.7394329834239386 2315 80-81 0.7648701622194878 2315 82-83 0.7619687336643608 2315 84-85 0.8569209067845378 2315 86-87 0.8613812177026716 2315 88-89 0.8728061966400595 2315 90-91 0.9372600734121903 2315 92-93 0.9407108970443332 2315 94-95 1.030272072140221 2315 96-97 1.1342538379255735 2315 98-99 1.1793445022345956 2315 100 1.148538535179462 2316 1 0.2834861733055334 2316 2 0.1284924454951195 2316 3 0.22103379667618128 2316 4 0.018891950988894735 2316 5 0.2803605845401407 2316 6 0.35319215480578947 2316 7 0.4231772943732466 2316 8 0.43762903456538993 2316 9 0.6498770592972818 2316 10-11 0.6186000087334804 2316 12-13 0.3976965470252338 2316 14-15 0.5287980196425011 2316 16-17 0.7999087379827188 2316 18-19 0.7663406818683001 2316 20-21 0.7547897520752329 2316 22-23 0.8083401771379499 2316 24-25 0.4780080063858634 2316 26-27 0.7384651641710533 2316 28-29 0.9006958547117847 2316 30-31 0.5524340802836463 2316 32-33 0.7223068726001074 2316 34-35 0.8423575302230475 2316 36-37 0.8703352638270516 2316 38-39 0.7407647769032408 2316 40-41 0.7245309043185699 2316 42-43 0.737485387383991 2316 44-45 0.572131700418133 2316 46-47 0.6825337830502463 2316 48-49 0.7512580841104821 2316 50-51 0.789168881881622 2316 52-53 0.7784588304596838 2316 54-55 0.6722476892124476 2316 56-57 0.837100083153274 2316 58-59 0.7426104399351772 2316 60-61 0.8568445168904262 2316 62-63 0.8764204189487756 2316 64-65 0.9558359071826956 2316 66-67 0.938324765227307 2316 68-69 0.8967778270636728 2316 70-71 0.8617194415150209 2316 72-73 0.8880294643820008 2316 74-75 0.9067625504748236 2316 76-77 0.8141389342953538 2316 78-79 0.5522454561836483 2316 80-81 0.5982960506726656 2316 82-83 0.46294777613960747 2316 84-85 0.555339365732479 2316 86-87 0.634148488259271 2316 88-89 0.6442460869594662 2316 90-91 0.5089446667328694 2316 92-93 0.36392608384827696 2316 94-95 0.4389162252498622 2316 96-97 0.6144792910325201 2316 98-99 0.8195845695414512 2316 100 0.4260556767837258 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 3.0 11 6.0 12 24.0 13 154.0 14 564.0 15 1557.0 16 3706.0 17 7835.0 18 13278.0 19 19656.0 20 28134.0 21 39167.0 22 48510.0 23 50435.0 24 51338.0 25 54975.0 26 64193.0 27 78928.0 28 97199.0 29 120350.0 30 148091.0 31 186366.0 32 236717.0 33 311644.0 34 445153.0 35 619323.0 36 964609.0 37 1422727.0 38 1167401.0 39 178569.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.36008352396124 20.283343525320166 13.68824725508404 23.66832569563456 2 15.564837321650613 19.933012808973466 40.68251847815441 23.819631391221517 3 19.077828745912935 25.522641742886975 29.31241440495021 26.087115106249883 4 12.66428565474656 16.358429475772137 38.228153450236626 32.74913141924468 5 13.313295451276048 37.779766403238426 34.391369770772314 14.515568374713217 6 31.178522090381456 37.022481616962125 17.508437464969997 14.290558827686425 7 27.871015637946 31.88238005683153 22.08988399438162 18.15672031084085 8 26.479734126505612 35.610464956319504 19.451455903898662 18.458345013276222 9 26.311461348822956 15.813481122494466 20.85936963186832 37.015687896814256 10-11 25.919098876260172 26.155892891407976 27.047791074147742 20.87721715818411 12-13 25.91199267807054 23.60829375319397 27.814724307078 22.664989261657496 14-15 23.135273189206696 25.023433287058943 25.660832844648546 26.180460679085808 16-17 22.206274218426845 27.77104710702652 26.688665111991778 23.33401356255486 18-19 21.81051089524463 27.24817348751749 28.982218554514898 21.959097062722986 20-21 22.91028773381079 26.490615653179013 28.6907554666264 21.9083411463838 22-23 22.944787291928662 26.278574459498362 28.39850178103735 22.37813646753563 24-25 22.505060462205915 26.196977164836255 29.017551058589735 22.280411314368095 26-27 22.47022505221803 26.505416438929686 28.393401524591148 22.63095698426113 28-29 22.00298540994675 26.831672575568927 28.713884797512435 22.45145721697189 30-31 22.7879086789751 26.477364933967856 28.64065084570101 22.09407554135603 32-33 22.882020875647576 26.603226682436905 28.139283882988064 22.37546855892745 34-35 22.80775017255123 26.61858087011111 28.59056348808087 21.983105469256788 36-37 22.635885097371634 26.44940473639584 28.34464875307327 22.570061413159248 38-39 22.707698428423612 26.535549957384713 28.633904787065077 22.122846827126597 40-41 22.565533060924167 25.966122319995343 28.966666299820925 22.501678319259558 42-43 22.50105178492146 26.132594704663823 28.493466670523738 22.87288683989098 44-45 22.47465025460793 26.753011634738506 28.344504802884146 22.427833307769422 46-47 22.464910150281682 26.564243352523487 27.86681404897566 23.104032448219176 48-49 22.96661129015737 26.52976067458666 27.889769799801066 22.613858235454902 50-51 22.71495939035971 26.59458356260254 27.747081451124522 22.943375595913228 52-53 22.439724356716116 26.916683182946738 27.554847087286856 23.08874537305029 54-55 22.404315006207995 26.778016694957007 27.65600391638638 23.16166438244862 56-57 22.96107145414745 26.599892981617508 27.300276177231687 23.138759387003358 58-59 22.34403238890841 26.888795476345145 27.79576541390173 22.97140672084472 60-61 22.548241884427643 27.2022698234373 27.41197630275905 22.837511989376004 62-63 22.732522084084483 26.80374614054717 27.181883492378255 23.28184828299009 64-65 22.208051712138904 26.840212164462844 27.375097805615518 23.576638317782734 66-67 22.448328502731606 26.637312843024112 27.364388669837286 23.549969984406992 68-69 22.20090199882928 26.6944963095257 27.501313166700513 23.603288524944503 70-71 22.21503705738714 26.366606422965834 27.611353647564833 23.80700287208219 72-73 22.536357938865986 26.58863003822714 27.488002456251365 23.387009566655507 74-75 22.66934777910972 26.821224080753588 27.18467780569227 23.324750334444424 76-77 22.5305098344131 26.639275264094593 27.031895327947055 23.798319573545253 78-79 22.31936820708736 26.656470033447775 27.187697117852487 23.836464641612373 80-81 22.087024249007435 26.98188473982356 27.330714691451945 23.600376319717057 82-83 22.72731883872769 26.415284410271127 27.84302731353055 23.014369437470634 84-85 22.855979501754458 26.643456337173426 27.110672625029746 23.389891536042366 86-87 22.052695707807107 27.019711239306922 27.413734668028873 23.5138583848571 88-89 22.47413508283381 26.268875568351664 27.381790720724975 23.875198628089553 90-91 22.462443927099507 26.293399699455467 27.418856947675884 23.825299425769142 92-93 22.610670073789095 26.42998364200616 27.319328156015043 23.640018128189705 94-95 21.88713268166823 26.38388291255353 27.65440265469158 24.074581751086658 96-97 22.062806707924633 26.3121311398087 27.437982355634777 24.18707979663189 98-99 21.71929887186432 26.60880674065019 27.321143818448473 24.35075056903701 100 22.0218076408994 26.278595734247666 27.035256362370504 24.664340262482433 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 995.0 1 723.0 2 889.5 3 1257.0 4 926.0 5 707.5 6 1090.5 7 2148.5 8 3331.0 9 3853.0 10 3494.5 11 3031.0 12 3240.5 13 3878.0 14 4600.0 15 5414.0 16 6259.5 17 7062.0 18 7779.0 19 8451.0 20 9317.5 21 10338.5 22 11565.5 23 13145.0 24 15363.5 25 18292.0 26 22249.5 27 26817.0 28 32843.0 29 42442.5 30 55286.0 31 68687.0 32 86162.0 33 104596.5 34 119625.0 35 135483.0 36 152127.5 37 175109.0 38 187974.5 39 185259.5 40 180965.5 41 176350.5 42 177362.5 43 186903.0 44 202365.0 45 226981.0 46 247931.5 47 262303.0 48 274491.0 49 276360.5 50 272034.5 51 274067.5 52 272316.0 53 260833.5 54 250005.0 55 225499.0 56 198731.0 57 170699.0 58 143250.5 59 128705.5 60 106185.5 61 79529.5 62 61073.0 63 48172.0 64 34884.0 65 21957.0 66 14912.0 67 10684.5 68 8154.5 69 6347.5 70 4979.0 71 3766.0 72 2871.0 73 2364.5 74 1789.5 75 1251.5 76 843.0 77 490.0 78 313.5 79 196.0 80 129.0 81 86.5 82 55.0 83 33.5 84 24.5 85 19.0 86 10.5 87 6.5 88 5.0 89 5.0 90 4.0 91 2.0 92 1.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04218142919737843 2 0.0 3 0.0 4 0.03549968957423798 5 0.0 6 0.0 7 0.0010219131188332457 8 0.0 9 0.011759861736727197 10-11 4.323478579679116E-4 12-13 7.86087014487112E-6 14-15 1.4149566260768018E-4 16-17 2.8299132521536035E-4 18-19 0.0 20-21 7.86087014487112E-6 22-23 0.0 24-25 5.345391698512362E-4 26-27 4.480695982576538E-4 28-29 2.9085219536023144E-4 30-31 0.0024525914851997897 32-33 0.015092870678152553 34-35 0.02575221059459779 36-37 0.03090108053948837 38-39 0.021994714665349396 40-41 0.0013992348857870594 42-43 4.7165220869226717E-5 44-45 0.011673392165133613 46-47 0.018779618776097105 48-49 0.003466643733888164 50-51 0.011594783463684902 52-53 0.011516174762236191 54-55 0.01060431382543114 56-57 4.6379133854739614E-4 58-59 0.008497600626605682 60-61 0.004724382957067543 62-63 0.001564313158829353 64-65 0.03685175923915581 66-67 0.06167638715665881 68-69 0.060497256634928144 70-71 0.05321023001063261 72-73 0.04659137734865113 74-75 0.0363643852901738 76-77 0.032300315425275436 78-79 0.01577676638075634 80-81 0.0011005218202819569 82-83 0.039406542036238924 84-85 0.05332814306280568 86-87 0.059168769580444924 88-89 0.07104654436934518 90-91 0.04965711670515087 92-93 0.04033412471333372 94-95 0.013512835779033457 96-97 0.0036395828770753283 98-99 0.02217551467868143 100 0.03178935886585881 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6360619.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.62107020664008 #Duplication Level Percentage of deduplicated Percentage of total 1 78.39263945337198 52.22601536706919 2 13.568079942706094 18.078400128646557 3 4.055996065746878 8.106443959619362 4 1.6114190392850138 4.294178437940937 5 0.7780807966083124 2.5918287688640413 6 0.44126009980648384 1.7638332053158063 7 0.26752186490193275 1.2475815060410063 8 0.17950605021090138 0.9567108138893698 9 0.12519611435375633 0.7506629211564128 >10 0.5439437453089018 6.133052638737418 >50 0.024863970494503746 1.1465737213671936 >100 0.01060437689312986 1.2972063667262834 >500 5.158933946488139E-4 0.2248708904607624 >1k 2.8660532107925747E-4 0.3283969013557246 >5k 2.866053210205252E-5 0.188228190776761 >10k+ 5.732106420410504E-5 0.6660161820332928 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19045 0.299420543818141 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15836 0.24896947922835813 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9858 0.15498491577627901 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.572174028974224E-5 0.0 3 0.0 0.0 0.0 1.572174028974224E-5 0.0 4 0.0 0.0 0.0 1.572174028974224E-5 3.144348057948448E-5 5 0.0 0.0 0.0 1.572174028974224E-5 3.144348057948448E-5 6 0.0 0.0 0.0 1.572174028974224E-5 4.716522086922672E-5 7 0.0 0.0 0.0 1.572174028974224E-5 4.716522086922672E-5 8 0.0 0.0 0.0 1.572174028974224E-5 4.716522086922672E-5 9 0.0 0.0 0.0 3.144348057948448E-5 4.716522086922672E-5 10-11 0.0 0.0 0.0 6.288696115896896E-5 4.716522086922672E-5 12-13 0.0 0.0 0.0 9.433044173845345E-5 4.716522086922672E-5 14-15 0.0 0.0 0.0 9.433044173845345E-5 7.86087014487112E-5 16-17 0.0 0.0 0.0 9.433044173845345E-5 7.86087014487112E-5 18-19 0.0 0.0 0.0 1.179130521730668E-4 1.1005218202819568E-4 20-21 0.0 0.0 0.0 1.493565327525513E-4 1.1005218202819568E-4 22-23 0.0 0.0 0.0 2.987130655051026E-4 1.2577392231793793E-4 24-25 0.0 0.0 0.0 6.445913518794319E-4 1.4149566260768018E-4 26-27 0.0 0.0 0.0 0.0015564522886844818 1.4149566260768018E-4 28-29 0.0 0.0 0.0 0.004441391631852183 1.493565327525513E-4 30-31 0.0 0.0 0.0 0.015006401106558969 1.572174028974224E-4 32-33 0.0 0.0 0.0 0.04141106392318106 1.572174028974224E-4 34-35 0.0 0.0 0.0 0.08219325823477244 1.572174028974224E-4 36-37 0.0 0.0 0.0 0.14809093265922701 1.7293914318716465E-4 38-39 0.0 0.0 0.0 0.24550283549446994 1.96521753621778E-4 40-41 0.0 0.0 0.0 0.35152239113834677 2.358261043461336E-4 42-43 0.0 0.0 0.0 0.45386305955442385 2.5154784463587586E-4 44-45 0.0 0.0 0.0 0.5726879726642957 2.5154784463587586E-4 46-47 0.0 0.0 0.0 0.7142465222331349 2.59408714780747E-4 48-49 0.0 0.0 0.0 0.8693257684511524 2.672695849256181E-4 50-51 0.0 0.0 0.0 1.025379762567134 2.672695849256181E-4 52-53 0.0 0.0 0.0 1.1779278085985028 3.065739356499737E-4 54-55 0.0 0.0 0.0 1.3451835426709255 3.458782863743293E-4 56-57 0.0 0.0 0.0 1.5400545135622807 3.5373915651920043E-4 58-59 0.0 0.0 0.0 1.7605440602557707 3.616000266640715E-4 60-61 0.0 0.0 0.0 1.985034789853 3.616000266640715E-4 62-63 0.0 0.0 0.0 2.212779919690206 4.0876524753329823E-4 64-65 0.0 0.0 0.0 2.4414526322045074 4.323478579679116E-4 66-67 0.0 0.0 0.0 2.696726529289052 4.6379133854739614E-4 68-69 0.0 0.0 0.0 2.9773202891102266 4.716522086922672E-4 70-71 0.0 0.0 0.0 3.2802860853637044 4.8737394898200944E-4 72-73 0.0 0.0 0.0 3.5819076728224095 5.659826504307207E-4 74-75 0.0 0.0 0.0 3.877264146775652 5.659826504307207E-4 76-77 0.0 0.0 0.0 4.206603162365172 5.659826504307207E-4 78-79 0.0 0.0 0.0 4.5571586664757 5.659826504307207E-4 80-81 0.0 0.0 0.0 4.926147911075951 5.895652608653341E-4 82-83 0.0 0.0 0.0 5.30468811290222 6.445913518794319E-4 84-85 0.0 0.0 0.0 5.679690923163296 6.445913518794319E-4 86-87 0.0 0.0 0.0 6.073292552187137 6.445913518794319E-4 88 0.0 0.0 0.0 6.394471984566282 6.445913518794319E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10525 0.0 48.379383 1 GTATCAA 18605 0.0 39.852543 1 GTACTAG 4025 0.0 33.29147 1 ATCAACG 22125 0.0 32.294014 3 CAACGCA 22355 0.0 31.927921 5 TACCTGG 4325 0.0 30.645256 2 TATCAAC 24120 0.0 30.554066 2 AACGCAG 24985 0.0 29.338156 6 TCAACGC 25020 0.0 28.764023 4 GTGGTAT 4055 0.0 26.668032 1 ACGCAGA 27375 0.0 26.12471 7 TAGGCAT 5565 0.0 26.005972 5 GTACATG 25200 0.0 25.933924 1 CGCAGAG 27890 0.0 25.60821 8 TACATGG 25365 0.0 25.218746 2 CTGTGCG 5560 0.0 24.50797 9 GCATCGT 5120 0.0 24.411575 8 ACATGGG 25225 0.0 24.39328 3 CTAGGCA 6100 0.0 24.351124 4 GGGCACC 5105 0.0 24.128984 94 >>END_MODULE