FastQCFastQC Report
Mon 6 Jun 2016
ERR1211256_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211256_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7627110
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239090.3134739108259878No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185410.24309338661694926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109990.1442092745482889No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA159300.043.575761
GTATCAA274000.038.6456531
GTACTAG87250.036.7864341
GGGCACC78150.035.96164394
GCATCGT79950.035.7470938
CAACGCA302400.032.924545
TACCCCG5400.032.209475
ATCAACG309800.032.1987883
TAGGCAT102750.032.0251965
TATCAAC337500.031.1512362
CTAGGCA113200.030.0239264
AACGCAG350500.029.3582736
CTGTGCG106800.029.0499
TACCTGG37050.028.5798652
TAGTACT115350.027.8341964
TCAACGC369750.027.410454
GTGGTAT55900.027.361521
ACGCAGA366150.027.1792437
CGCAGAG367150.027.129588
ACTGTGC121100.026.8995328
Produced by FastQC (version 0.11.5)