##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211256_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7627110 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.625337119826515 31.0 31.0 34.0 30.0 34.0 2 31.64801661441883 33.0 31.0 34.0 30.0 34.0 3 31.71691702361707 33.0 31.0 34.0 30.0 34.0 4 35.305835106613124 37.0 35.0 37.0 33.0 37.0 5 35.37244788655205 37.0 35.0 37.0 33.0 37.0 6 35.43283379943386 37.0 35.0 37.0 33.0 37.0 7 35.356522195169596 37.0 35.0 37.0 32.0 37.0 8 35.36508113820307 37.0 35.0 37.0 33.0 37.0 9 36.94595528319377 39.0 37.0 39.0 33.0 39.0 10-11 36.90639881947422 39.0 37.0 39.0 33.0 39.0 12-13 36.80014173127174 39.0 37.0 39.0 32.0 39.0 14-15 38.01845725314044 40.0 38.0 41.0 33.0 41.0 16-17 38.00600856680971 40.0 38.0 41.0 33.0 41.0 18-19 38.02948357897028 40.0 38.0 41.0 33.0 41.0 20-21 37.998022776648035 40.0 38.0 41.0 33.0 41.0 22-23 37.888016103084915 40.0 38.0 41.0 32.0 41.0 24-25 37.79452715379744 40.0 37.0 41.0 32.0 41.0 26-27 37.65002169891348 40.0 37.0 41.0 32.0 41.0 28-29 37.54003495426184 40.0 37.0 41.0 32.0 41.0 30-31 37.39406492105135 40.0 36.5 41.0 31.5 41.0 32-33 37.22705257954848 40.0 36.0 41.0 31.0 41.0 34-35 37.091807644048664 39.5 36.0 41.0 31.0 41.0 36-37 36.99408347329461 39.0 36.0 41.0 30.5 41.0 38-39 36.85545173728974 39.0 35.0 41.0 30.0 41.0 40-41 36.68786539069189 39.0 35.0 41.0 30.0 41.0 42-43 36.47382330922197 39.0 35.0 40.5 30.0 41.0 44-45 36.224974269415284 39.0 35.0 40.0 29.0 41.0 46-47 35.96929223519787 38.0 35.0 40.0 28.0 41.0 48-49 35.74339114028774 38.0 35.0 40.0 27.0 41.0 50-51 35.03792760298462 37.5 33.5 39.5 26.5 40.5 52-53 35.113947943585444 38.0 34.0 40.0 26.5 40.5 54-55 35.568035140439825 38.0 34.0 40.0 28.0 41.0 56-57 35.49548944488804 38.0 34.0 40.0 27.5 41.0 58-59 35.26080376446649 37.5 34.0 40.0 27.0 41.0 60-61 35.038864655157724 37.0 34.0 40.0 27.0 41.0 62-63 34.73656089921346 36.5 34.0 40.0 26.0 41.0 64-65 34.48850652475184 36.0 34.0 39.5 26.0 41.0 66-67 34.32568154910575 36.0 33.5 39.0 26.0 41.0 68-69 34.05004818338794 35.0 33.0 39.0 26.0 41.0 70-71 33.79064586717642 35.0 33.0 39.0 26.0 41.0 72-73 33.49089478190298 35.0 33.0 37.5 25.0 40.0 74-75 33.179099554090605 35.0 33.0 37.0 25.0 39.5 76-77 32.87898994507749 35.0 32.0 36.5 24.5 39.0 78-79 32.56312974376927 35.0 32.0 36.0 24.0 39.0 80-81 32.2894085702186 35.0 32.0 36.0 24.0 38.5 82-83 32.00472721646862 35.0 31.5 35.5 23.0 37.0 84-85 31.774011453879645 35.0 31.0 35.0 23.0 37.0 86-87 31.567388958596375 35.0 31.0 35.0 23.0 36.5 88-89 31.38041637002744 34.5 31.0 35.0 21.0 36.0 90-91 31.16185534232494 34.0 31.0 35.0 20.0 36.0 92-93 30.970399338674806 34.0 31.0 35.0 20.0 36.0 94-95 30.819955330393817 34.0 30.5 35.0 19.5 35.0 96-97 30.644156305599367 34.0 30.0 35.0 19.0 35.0 98-99 30.42648041001113 34.0 30.0 35.0 18.5 35.0 100 29.275485341105608 33.0 27.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4863880030813199 1101 2 -0.3174247443747191 1101 3 -0.42473616116598834 1101 4 -0.36683908768037554 1101 5 -0.3083017236159904 1101 6 -0.27009702670513747 1101 7 -0.32061971678812284 1101 8 -0.33359105523478405 1101 9 -0.46994868277636925 1101 10-11 -0.4930359595842475 1101 12-13 -0.469010303958207 1101 14-15 -0.6132295890621435 1101 16-17 -0.6342941018278836 1101 18-19 -0.5305965518741473 1101 20-21 -0.5944176256390605 1101 22-23 -0.6712181305970617 1101 24-25 -0.7796301549462115 1101 26-27 -0.7673296500090672 1101 28-29 -0.7916624942182651 1101 30-31 -0.8439711578266369 1101 32-33 -0.8151726278909166 1101 34-35 -0.8239289150838971 1101 36-37 -0.870857081344333 1101 38-39 -0.8126265069654437 1101 40-41 -0.8235416014245232 1101 42-43 -0.9165833430626904 1101 44-45 -0.8909456021636757 1101 46-47 -1.0406544926520525 1101 48-49 -1.143121849529038 1101 50-51 -1.0663418302946965 1101 52-53 -0.9620705083324879 1101 54-55 -0.8626760113569176 1101 56-57 -0.7711878195042559 1101 58-59 -0.788352569110053 1101 60-61 -0.7520167667039601 1101 62-63 -0.7954513112034292 1101 64-65 -0.7814632731322178 1101 66-67 -0.821989009071423 1101 68-69 -0.7745543990712704 1101 70-71 -0.9186872230704708 1101 72-73 -0.8602740698979474 1101 74-75 -0.9480105093791167 1101 76-77 -0.9799323372245681 1101 78-79 -0.9247808102605894 1101 80-81 -0.9986214855567113 1101 82-83 -1.0350320263521589 1101 84-85 -1.0276111819235325 1101 86-87 -1.0931570418807333 1101 88-89 -1.1601307594598786 1101 90-91 -1.2302063595647716 1101 92-93 -1.3710431488590622 1101 94-95 -1.4216331470600885 1101 96-97 -1.4638613676356975 1101 98-99 -1.6139162170091836 1101 100 -1.617068272000413 1102 1 -0.4671742286960452 1102 2 -0.3899736588695326 1102 3 -0.40512380400802783 1102 4 -0.2535371446017649 1102 5 -0.3045655685350326 1102 6 -0.2791159174438249 1102 7 -0.29286008950024467 1102 8 -0.4313830158748573 1102 9 -0.3900620728742439 1102 10-11 -0.4722559484854827 1102 12-13 -0.4757569254356113 1102 14-15 -0.5539779517952965 1102 16-17 -0.6264402342844377 1102 18-19 -0.62297979888924 1102 20-21 -0.77025755320917 1102 22-23 -0.7686364757290391 1102 24-25 -0.7696202176770797 1102 26-27 -0.7621708572135404 1102 28-29 -0.8468435242593912 1102 30-31 -0.828415162158926 1102 32-33 -0.8991275617301469 1102 34-35 -0.8755117800948824 1102 36-37 -0.8539715167912973 1102 38-39 -0.9974874100160136 1102 40-41 -1.0362593204390578 1102 42-43 -0.9882822289171287 1102 44-45 -1.0711262102927819 1102 46-47 -1.084156203629405 1102 48-49 -0.9710292452993983 1102 50-51 -1.021851951716016 1102 52-53 -0.9417935652786937 1102 54-55 -0.8527148173359507 1102 56-57 -0.9045912398706051 1102 58-59 -0.8468643215936282 1102 60-61 -0.784152217340754 1102 62-63 -0.8367304889018499 1102 64-65 -0.8809659133087635 1102 66-67 -0.8910065695463132 1102 68-69 -0.890381609716556 1102 70-71 -0.9875828757882275 1102 72-73 -0.9427081349756463 1102 74-75 -0.9996603839471518 1102 76-77 -1.1145440639963269 1102 78-79 -1.0397531278680177 1102 80-81 -1.1222716897124947 1102 82-83 -1.0748997842322332 1102 84-85 -1.199481711517432 1102 86-87 -1.2479204286529466 1102 88-89 -1.2502897855479382 1102 90-91 -1.2919788963679117 1102 92-93 -1.3084295676598607 1102 94-95 -1.2788520121862277 1102 96-97 -1.379317146788079 1102 98-99 -1.5102907517891282 1102 100 -1.6894628163304617 1103 1 -0.5907842480613574 1103 2 -0.5024602191022751 1103 3 -0.5529790129154684 1103 4 -0.5181642795295005 1103 5 -0.3936411836020284 1103 6 -0.47377223586617134 1103 7 -0.4603760591060464 1103 8 -0.4382153869660286 1103 9 -0.6963914879695494 1103 10-11 -0.6553728902720906 1103 12-13 -0.6936317405917549 1103 14-15 -0.867884534495424 1103 16-17 -0.9070874211943263 1103 18-19 -0.7796474293039211 1103 20-21 -0.7854281516665722 1103 22-23 -0.8571330890582729 1103 24-25 -0.9011171592829825 1103 26-27 -0.9602775441189166 1103 28-29 -0.9546977694188215 1103 30-31 -0.9356273102026691 1103 32-33 -0.9599712327668115 1103 34-35 -0.9831523442488788 1103 36-37 -1.0366284194858792 1103 38-39 -1.0939820777789535 1103 40-41 -1.0066407839506581 1103 42-43 -1.0915463229789353 1103 44-45 -1.1366012928889901 1103 46-47 -1.1312508045323213 1103 48-49 -1.1776387496618597 1103 50-51 -1.3083784532806035 1103 52-53 -1.1507497960602038 1103 54-55 -0.9084967030896465 1103 56-57 -0.8534201354454893 1103 58-59 -0.9230752559172117 1103 60-61 -0.9220142628356882 1103 62-63 -0.9777224566644804 1103 64-65 -0.9643319237232646 1103 66-67 -0.9762235784941069 1103 68-69 -1.0008170431170527 1103 70-71 -0.9470041149874717 1103 72-73 -1.061953128756464 1103 74-75 -1.0848825128062884 1103 76-77 -1.091550090433138 1103 78-79 -1.0921871939510162 1103 80-81 -1.126649063318446 1103 82-83 -1.114364060803819 1103 84-85 -1.1168716409530113 1103 86-87 -1.232294459257968 1103 88-89 -1.2641792014865239 1103 90-91 -1.277763914302657 1103 92-93 -1.420242106623892 1103 94-95 -1.4907899889779728 1103 96-97 -1.581711307679786 1103 98-99 -1.5027391837409922 1103 100 -1.5216601316898952 1104 1 -0.524030199967985 1104 2 -0.551593269256152 1104 3 -0.5790501424574046 1104 4 -0.4232378820306266 1104 5 -0.4598433086853291 1104 6 -0.3997468510608755 1104 7 -0.4658846243124444 1104 8 -0.4411624331143855 1104 9 -0.5654211063254238 1104 10-11 -0.594427615321095 1104 12-13 -0.5748678902231674 1104 14-15 -0.8349763828040935 1104 16-17 -0.9099735713854997 1104 18-19 -0.9039248237936519 1104 20-21 -0.8566356474963683 1104 22-23 -0.8832831447101697 1104 24-25 -0.8883508389013102 1104 26-27 -0.9290230697243871 1104 28-29 -0.9461088763464645 1104 30-31 -0.9951774873518673 1104 32-33 -1.0011409359822423 1104 34-35 -1.1075359163049683 1104 36-37 -1.0735913227411444 1104 38-39 -1.0218495644051089 1104 40-41 -1.0254385439693223 1104 42-43 -1.0817699734720847 1104 44-45 -1.034287570587523 1104 46-47 -1.103366672421977 1104 48-49 -1.0659129046162974 1104 50-51 -1.1157178449809564 1104 52-53 -0.9685906911571251 1104 54-55 -0.9102414149813569 1104 56-57 -0.9825453136110909 1104 58-59 -0.8958148138956403 1104 60-61 -0.9472274281468458 1104 62-63 -1.061655380960822 1104 64-65 -1.00455831409257 1104 66-67 -1.0607870573001392 1104 68-69 -1.1602688498738871 1104 70-71 -0.991964628363668 1104 72-73 -1.024754772917838 1104 74-75 -1.043227026602004 1104 76-77 -0.9394953600811462 1104 78-79 -1.0080234129926247 1104 80-81 -1.1097451174843265 1104 82-83 -1.1052875286974775 1104 84-85 -1.0327049433522575 1104 86-87 -1.0758405778551285 1104 88-89 -1.1803701608379562 1104 90-91 -1.172893121559266 1104 92-93 -1.2425342693407195 1104 94-95 -1.1637352438954593 1104 96-97 -1.355651933090403 1104 98-99 -1.4064267035167077 1104 100 -1.3247292054408781 1105 1 -0.5116204236072974 1105 2 -0.5286650789794933 1105 3 -0.5779427133138739 1105 4 -0.40071267591861925 1105 5 -0.31413026396219834 1105 6 -0.3530811225727035 1105 7 -0.3662092421838281 1105 8 -0.37861242340139967 1105 9 -0.6009707714538308 1105 10-11 -0.5813711852362857 1105 12-13 -0.5252604854803877 1105 14-15 -0.750213645307376 1105 16-17 -0.7205235643356431 1105 18-19 -0.8074335708211109 1105 20-21 -0.7122151667082761 1105 22-23 -0.7306461669318836 1105 24-25 -0.8197781693387256 1105 26-27 -0.7494738198819064 1105 28-29 -0.8770700915877327 1105 30-31 -0.8858581980369493 1105 32-33 -0.9720428026465342 1105 34-35 -0.8875305576024317 1105 36-37 -0.9271736302628071 1105 38-39 -0.9264749331031723 1105 40-41 -1.0041688613156339 1105 42-43 -1.0048457578819097 1105 44-45 -1.0424372865722304 1105 46-47 -1.1245325335934595 1105 48-49 -1.113742231292072 1105 50-51 -1.055546131278966 1105 52-53 -0.8996389650371128 1105 54-55 -0.7791455697737817 1105 56-57 -0.7843697057924217 1105 58-59 -0.7349306523088615 1105 60-61 -0.7377198677664794 1105 62-63 -0.8011414619202171 1105 64-65 -0.8037336362669905 1105 66-67 -0.760465846071142 1105 68-69 -0.7482564745695015 1105 70-71 -0.759633915846841 1105 72-73 -0.7172562258102673 1105 74-75 -0.7776441048032368 1105 76-77 -0.8438555649284964 1105 78-79 -0.8235065485581998 1105 80-81 -0.8894840578265857 1105 82-83 -0.9728162516270054 1105 84-85 -0.9105599158267204 1105 86-87 -0.9548999741521378 1105 88-89 -1.058865095417584 1105 90-91 -1.0587259232806439 1105 92-93 -1.0803995861118203 1105 94-95 -1.1427576267525836 1105 96-97 -1.1656143478212648 1105 98-99 -1.2239912705426015 1105 100 -1.224625018961273 1106 1 -0.5708098711076417 1106 2 -0.5056715515298258 1106 3 -0.6359194614819828 1106 4 -0.3969741484824283 1106 5 -0.4441450471680497 1106 6 -0.3795106091757674 1106 7 -0.4361869358443826 1106 8 -0.3875296673845128 1106 9 -0.559300787864693 1106 10-11 -0.534634310042982 1106 12-13 -0.7159282789741539 1106 14-15 -0.8730268047695944 1106 16-17 -0.8458522166675166 1106 18-19 -0.9025102568268935 1106 20-21 -0.8064614901874663 1106 22-23 -0.7802026029373295 1106 24-25 -0.7848633407438186 1106 26-27 -0.8829393391427018 1106 28-29 -0.9456152854754833 1106 30-31 -0.9489121252836625 1106 32-33 -0.9473077882156957 1106 34-35 -0.91440351363552 1106 36-37 -0.9811708738309832 1106 38-39 -0.9610439898434038 1106 40-41 -0.9995164871126789 1106 42-43 -0.8932343781279357 1106 44-45 -0.9663531410298916 1106 46-47 -0.8812067977080602 1106 48-49 -0.8989810102603641 1106 50-51 -0.9493986422126781 1106 52-53 -0.9422551383720901 1106 54-55 -0.802524452452424 1106 56-57 -0.7939190037998074 1106 58-59 -0.7732079758362147 1106 60-61 -0.8026868736294261 1106 62-63 -0.7827562836319757 1106 64-65 -0.8598742947176987 1106 66-67 -0.9146213104231933 1106 68-69 -0.8791913610841391 1106 70-71 -0.7534437122696573 1106 72-73 -0.7309460078111343 1106 74-75 -0.7856074888065407 1106 76-77 -0.657604479370697 1106 78-79 -0.7543442685871717 1106 80-81 -0.7160616917032598 1106 82-83 -0.7373752583548239 1106 84-85 -0.8570749349949516 1106 86-87 -0.8277110465083446 1106 88-89 -0.939502156203794 1106 90-91 -0.969847407197161 1106 92-93 -0.9517761145730965 1106 94-95 -1.0726043582844085 1106 96-97 -1.093925532900304 1106 98-99 -0.8733718852995302 1106 100 -1.0722109747265876 1107 1 -0.5533692781644071 1107 2 -0.5479418870911026 1107 3 -0.5057971260998784 1107 4 -0.43061920159881595 1107 5 -0.4045133481474892 1107 6 -0.43596962450786947 1107 7 -0.43938650990450867 1107 8 -0.4418133818843444 1107 9 -0.5707299298156627 1107 10-11 -0.6181682201793066 1107 12-13 -0.6242746735602438 1107 14-15 -0.8088026488295341 1107 16-17 -0.7510276251516075 1107 18-19 -0.7981242072234949 1107 20-21 -0.7976146181316608 1107 22-23 -0.8836529003537237 1107 24-25 -0.8123261867493881 1107 26-27 -0.8289288852472296 1107 28-29 -0.872304318125984 1107 30-31 -0.8565626317553807 1107 32-33 -0.949994303469694 1107 34-35 -0.9072279831962078 1107 36-37 -1.0331610116441396 1107 38-39 -0.945808189062511 1107 40-41 -0.9855302285292282 1107 42-43 -0.9427873313449382 1107 44-45 -0.9339091679699507 1107 46-47 -0.8594965113504429 1107 48-49 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58-59 1.104324439457777 2311 60-61 1.1162920343430471 2311 62-63 1.1055671792694781 2311 64-65 1.0507809797546273 2311 66-67 1.1043347202333678 2311 68-69 1.092550176647194 2311 70-71 1.0630505808797537 2311 72-73 1.063857057603606 2311 74-75 1.0491815435208167 2311 76-77 1.0765085849266072 2311 78-79 1.1079473060642115 2311 80-81 1.1335419694015911 2311 82-83 1.1874442413475883 2311 84-85 1.181336475645569 2311 86-87 1.1697066463893542 2311 88-89 1.2589596671407612 2311 90-91 1.2913941325202636 2311 92-93 1.2985206092468182 2311 94-95 1.3747499147878557 2311 96-97 1.3713183786769712 2311 98-99 1.5036168060171917 2311 100 1.4457732052043255 2312 1 0.6894899454844463 2312 2 0.6506959787886437 2312 3 0.7123255415132093 2312 4 0.36010490593987043 2312 5 0.4261246567176187 2312 6 0.5155659931285044 2312 7 0.4402015815317242 2312 8 0.5488526496274844 2312 9 0.7256725364299683 2312 10-11 0.6726056413273653 2312 12-13 0.7850131707116148 2312 14-15 1.0230841457305573 2312 16-17 0.9999252389551501 2312 18-19 1.0238922609908983 2312 20-21 1.027756105159959 2312 22-23 1.1257953898005653 2312 24-25 1.066957362316323 2312 26-27 1.1141903697788038 2312 28-29 1.1007803286138653 2312 30-31 1.1406371234070107 2312 32-33 1.1848527087017615 2312 34-35 1.1456055266092378 2312 36-37 1.1807174137013305 2312 38-39 1.1801392149049619 2312 40-41 1.2398976478395625 2312 42-43 1.2105392972889675 2312 44-45 1.2744233690930713 2312 46-47 1.303782438788872 2312 48-49 1.3319889465989618 2312 50-51 1.3527340269887063 2312 52-53 1.2263766467402917 2312 54-55 1.1391616200390544 2312 56-57 1.058940160375947 2312 58-59 1.0620781450477352 2312 60-61 1.0714621707490153 2312 62-63 1.0291578995664636 2312 64-65 1.0639616904107996 2312 66-67 1.0268286034076013 2312 68-69 1.0534624329773692 2312 70-71 1.0322152742782933 2312 72-73 1.0505378776356693 2312 74-75 1.0695031411060683 2312 76-77 1.0925775468177648 2312 78-79 1.1362628324953548 2312 80-81 1.1390340593486812 2312 82-83 1.1608312444087652 2312 84-85 1.175798798825408 2312 86-87 1.1742478750334548 2312 88-89 1.0923080221857226 2312 90-91 1.1435928878434147 2312 92-93 1.2180667956427165 2312 94-95 1.2675651588086438 2312 96-97 1.3286739654304434 2312 98-99 1.0478911814975724 2312 100 1.3403415807987358 2313 1 0.541689083826121 2313 2 0.5348716582477415 2313 3 0.5673796803758862 2313 4 0.41287567443080064 2313 5 0.2760473603055047 2313 6 0.41994400336245974 2313 7 0.48493586918440457 2313 8 0.4730324638213048 2313 9 0.6098232702026039 2313 10-11 0.6347866936621713 2313 12-13 0.6658976971161579 2313 14-15 0.8388116916065727 2313 16-17 0.867995308366261 2313 18-19 0.8583546448870081 2313 20-21 0.8724270950540998 2313 22-23 0.894440904206931 2313 24-25 0.8822016047257506 2313 26-27 0.9047489345607502 2313 28-29 0.9322346379319058 2313 30-31 0.9465193508514105 2313 32-33 1.009289968014464 2313 34-35 0.9629456868991042 2313 36-37 1.0465802124513814 2313 38-39 1.0729434830965445 2313 40-41 1.0570780622537512 2313 42-43 1.1022981452889624 2313 44-45 1.127979358130439 2313 46-47 1.1448424445235617 2313 48-49 1.1224201129405529 2313 50-51 1.1861698984478934 2313 52-53 1.0569818486868314 2313 54-55 0.9179645044621694 2313 56-57 0.895201892772171 2313 58-59 0.8515241232492201 2313 60-61 0.8563975257569325 2313 62-63 0.8495995591036021 2313 64-65 0.8455508082418177 2313 66-67 0.8370747188564778 2313 68-69 0.873460877585643 2313 70-71 0.8491849309369073 2313 72-73 0.8586554972644862 2313 74-75 0.8367705407960102 2313 76-77 0.6574782257848 2313 78-79 0.8408891355473074 2313 80-81 0.955745442353539 2313 82-83 0.9526870873333131 2313 84-85 0.9796255042661421 2313 86-87 0.9442715809910176 2313 88-89 1.0175802730433396 2313 90-91 1.0683129272575975 2313 92-93 1.1335360704261426 2313 94-95 1.1172748124038492 2313 96-97 1.1517550399487284 2313 98-99 1.1898821889361635 2313 100 1.4058431780461973 2314 1 0.552582264379275 2314 2 0.5220577597115614 2314 3 0.5736979417733821 2314 4 0.39203760050989445 2314 5 0.44382994321929914 2314 6 0.4635044463928395 2314 7 0.4738465610580107 2314 8 0.4874310681219427 2314 9 0.6705819333824792 2314 10-11 0.6529694604042433 2314 12-13 0.705708590671243 2314 14-15 0.8705626342474702 2314 16-17 0.863151032716793 2314 18-19 0.82625590100713 2314 20-21 0.8830326951479819 2314 22-23 0.911301660504769 2314 24-25 0.9043506038925884 2314 26-27 0.9087485545470813 2314 28-29 0.9441907281291648 2314 30-31 0.9550374648258213 2314 32-33 0.9802497711121774 2314 34-35 0.9726876407009968 2314 36-37 0.9849921514663507 2314 38-39 1.0112750921285425 2314 40-41 1.0733359760578907 2314 42-43 1.1219883670342838 2314 44-45 1.1067307296880742 2314 46-47 1.1520393398536157 2314 48-49 1.1311151690514478 2314 50-51 1.112252991762169 2314 52-53 1.065561245458241 2314 54-55 0.9189651258491196 2314 56-57 0.8721167913402823 2314 58-59 0.9373953291562884 2314 60-61 0.9390904595173737 2314 62-63 0.9880129836859695 2314 64-65 1.023984192967987 2314 66-67 1.04065500162681 2314 68-69 1.0218146018396226 2314 70-71 1.0495919500090665 2314 72-73 1.0128062083780662 2314 74-75 1.0142399405899596 2314 76-77 1.0202999713091359 2314 78-79 1.0304959854156053 2314 80-81 1.06722821971956 2314 82-83 1.0245846843828055 2314 84-85 1.0739414192715238 2314 86-87 1.1271213677938725 2314 88-89 1.150562893073701 2314 90-91 1.1720502930693577 2314 92-93 1.219660803113566 2314 94-95 1.2294247251569317 2314 96-97 1.2440579482962946 2314 98-99 1.3438340137451874 2314 100 1.4538698876932692 2315 1 0.4835061068742945 2315 2 0.49252711135778426 2315 3 0.5496010861030172 2315 4 0.23229904479186558 2315 5 0.34325574064091313 2315 6 0.3935121651398532 2315 7 0.44744370537625855 2315 8 0.4242686347079285 2315 9 0.5804684080346476 2315 10-11 0.48775303590522157 2315 12-13 0.5901649170629497 2315 14-15 0.8281205540593888 2315 16-17 0.7877032203142491 2315 18-19 0.6522865514579834 2315 20-21 0.7875298276926301 2315 22-23 0.5730864031797438 2315 24-25 0.7295972839925398 2315 26-27 0.8392701137130132 2315 28-29 0.8702155516974557 2315 30-31 0.8956858725290502 2315 32-33 0.9170350605254995 2315 34-35 0.9797287317570493 2315 36-37 0.9977050910701877 2315 38-39 1.0366501171811393 2315 40-41 0.9977058863358792 2315 42-43 1.1138871091466456 2315 44-45 1.1301645902269613 2315 46-47 1.1914043879051661 2315 48-49 1.2475848526352493 2315 50-51 1.0972099544674236 2315 52-53 1.0654752277894488 2315 54-55 0.9049183506616316 2315 56-57 0.8610698495848439 2315 58-59 0.8687108946919437 2315 60-61 0.8536119084368892 2315 62-63 0.8332477246683041 2315 64-65 0.9236913546710213 2315 66-67 0.8258004842050255 2315 68-69 0.8592811646709464 2315 70-71 0.9031653306824481 2315 72-73 0.6750838768581033 2315 74-75 0.8523355333696685 2315 76-77 0.8677652736464481 2315 78-79 0.9142021568481837 2315 80-81 0.6502997739873138 2315 82-83 0.6065375221345342 2315 84-85 0.8729445093167847 2315 86-87 1.012079944500666 2315 88-89 1.0145203701623622 2315 90-91 1.0918745796057756 2315 92-93 1.1761176630619694 2315 94-95 1.2002285421095351 2315 96-97 0.9743002664728415 2315 98-99 0.4848607868475767 2315 100 0.977896528679711 2316 1 0.4520730228433294 2316 2 0.39414246331264735 2316 3 0.4157780099677524 2316 4 0.2833355633804544 2316 5 0.24115185856842203 2316 6 0.2539385719444738 2316 7 0.24151050503560612 2316 8 0.3258965921970116 2316 9 0.3690826697519469 2316 10-11 0.3820779949415325 2316 12-13 0.1298656850014268 2316 14-15 0.1810007443359396 2316 16-17 0.3485870607214636 2316 18-19 0.4070422684789392 2316 20-21 0.44080532973165987 2316 22-23 0.398707678041859 2316 24-25 0.5085867170935572 2316 26-27 0.57087021309421 2316 28-29 0.47730729146149287 2316 30-31 0.40099765071799 2316 32-33 0.59999816674123 2316 34-35 0.5702718169575007 2316 36-37 0.5832248647469598 2316 38-39 0.761115698991226 2316 40-41 0.8160138647479371 2316 42-43 0.7817908058600906 2316 44-45 0.7077103938532119 2316 46-47 0.5269680416061249 2316 48-49 0.5380764674878904 2316 50-51 0.5348577963950589 2316 52-53 0.6664093359249534 2316 54-55 0.2803904935847683 2316 56-57 0.2135401574760607 2316 58-59 0.21298098958277478 2316 60-61 0.3079507910037762 2316 62-63 0.4759005049704612 2316 64-65 0.4054306918128674 2316 66-67 0.3595246762455204 2316 68-69 0.3829324977055748 2316 70-71 0.1729744858401503 2316 72-73 0.13297148733879993 2316 74-75 0.34682337382832884 2316 76-77 0.4133127080630601 2316 78-79 0.47290239837365533 2316 80-81 0.5726963433083299 2316 82-83 0.6481078920935879 2316 84-85 0.7220826725016742 2316 86-87 0.7101589519141349 2316 88-89 0.6814897108028326 2316 90-91 0.5554545338949488 2316 92-93 0.6237803196222167 2316 94-95 0.7994586753702819 2316 96-97 0.7176741557497586 2316 98-99 0.5200488717284095 2316 100 0.49057451710451616 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 20.0 1 497.0 2 154.0 3 158.0 4 149.0 5 115.0 6 115.0 7 488.0 8 1751.0 9 3379.0 10 5081.0 11 6865.0 12 8914.0 13 11072.0 14 13537.0 15 16209.0 16 19569.0 17 25160.0 18 31967.0 19 40278.0 20 52079.0 21 66038.0 22 73821.0 23 72515.0 24 69804.0 25 71205.0 26 78670.0 27 91614.0 28 109084.0 29 131065.0 30 161635.0 31 202638.0 32 260122.0 33 346256.0 34 507885.0 35 719318.0 36 1113147.0 37 1555996.0 38 1345013.0 39 413419.0 40 308.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18807014791229 20.995886886694766 13.1849203424564 22.631122622936537 2 15.367093466918144 19.605678684870906 42.00191313083625 23.025314717374698 3 19.26971295848984 26.61563461702883 28.448571139327615 25.66608128515371 4 12.299938091960387 16.405158417572284 39.96940921948531 31.32549427098202 5 12.984123703607148 38.56627599047522 34.562542242677345 13.887058063240287 6 29.60680929393481 38.38946198691249 17.930516268347617 14.073212450805084 7 27.260612548507197 32.10927271343823 22.863990932204057 17.766123805850512 8 26.115601592279237 35.60099935906904 19.57178308671615 18.711615961935575 9 26.058933345610402 15.782684845649294 21.892678209997968 36.265703598742334 10-11 25.88877888619916 26.40693483888366 26.77366876633645 20.930617508580735 12-13 25.940977153220707 24.00927837661332 27.675717996008114 22.374026474157862 14-15 23.277846179790913 25.121094293497396 25.699614027306982 25.90144549940471 16-17 21.55734716227998 28.045645647535878 26.904956874041204 23.492050316142944 18-19 20.88183702288227 28.07514118444589 29.45595591464002 21.587065878031826 20-21 22.650275619311536 27.094287448887535 28.91201302058557 21.343423911215364 22-23 23.030200061415318 26.861516588529238 28.512779481172362 21.595503868883082 24-25 22.36620117653485 26.781003512241043 29.378513068890033 21.474282242334077 26-27 22.26288346619489 27.08058519144771 28.323128899771522 22.333402442585882 28-29 21.36270673189523 27.46860680818989 29.0381418199357 22.130544639979185 30-31 22.299015802097983 27.10819489147085 28.965881790689817 21.62690751574135 32-33 22.54179128738941 27.323358342392535 28.064948496804092 22.069901873413958 34-35 22.574910852652692 27.213385917951786 28.79514725787074 21.41655597152478 36-37 22.158143220285027 26.781070329014184 28.411207440170134 22.649579010530655 38-39 22.347877955500284 27.01834753804178 28.91001590788338 21.723758598574552 40-41 22.157298440345116 26.0035746039061 29.516199649306664 22.32292730644212 42-43 22.082784646117975 26.23120427283208 28.874208265742695 22.811802815307246 44-45 22.35673769144653 27.053187589234597 28.623349180500966 21.96672553881791 46-47 22.27076455452319 26.993428978292304 27.930770913830337 22.805035553354166 48-49 22.954409699641946 26.894449841120853 27.95983071429115 22.191309744946047 50-51 22.601084426712013 26.69792413734358 28.023585202290825 22.67740623365358 52-53 22.090651317296135 27.27209351069894 27.75262702409462 22.884628147910306 54-55 22.0113142434958 27.18129513415916 27.73418643207588 23.07320419026916 56-57 22.948309629638956 26.756377909709805 27.35029501078681 22.94501744986443 58-59 21.964158398140228 27.215296012579653 28.129755499877128 22.690790089402988 60-61 22.008197688752734 27.806210100035145 27.460768859536387 22.72482335167573 62-63 22.435336578703662 27.077743119664305 27.110306548022827 23.376613753609206 64-65 21.739764490496604 27.206324580304443 27.559805224014678 23.49410570518428 66-67 22.222033505619933 26.88158194234807 27.482295304220163 23.414089247811837 68-69 21.805584748561056 26.92192848502456 27.74510456524683 23.52738220116755 70-71 21.7801370982863 26.556147790386515 27.83736743168384 23.826347679643344 72-73 22.415916824164363 26.614158179870774 27.978005042593406 22.991919953371458 74-75 22.638603032955587 26.850469399722975 27.552141116769175 22.95878645055226 76-77 22.279532677330284 26.828492496285666 27.197706491888724 23.69426833449533 78-79 22.184228081469357 26.875621837048435 27.280318338604836 23.659831742877373 80-81 21.74467153707777 27.284216644509957 27.843944096629752 23.12716772178252 82-83 22.69216013644683 26.359516175554642 28.824340116748537 22.12398357124999 84-85 22.746577108198966 26.681850379627793 27.663931255518836 22.907641256654404 86-87 21.558114141343545 27.30913620388982 28.083231303011893 23.04951835175474 88-89 22.571992753974783 26.08384176766507 28.032430605041007 23.311734873319136 90-91 22.247811829571905 26.34504280806031 27.95391615084746 23.45322921152033 92-93 22.479536697561713 26.462199312264133 27.98327266489911 23.07499132527504 94-95 21.476241181418896 26.366353452986246 28.698128881524816 23.45927648407005 96-97 21.832705055513557 26.257363814069635 28.224273706840446 23.685657423576362 98-99 21.306232934381466 26.673230618309816 28.074808065070332 23.94572838223838 100 22.047585292009597 26.11672252579892 27.335135776708615 24.50055640548287 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 449.0 1 334.0 2 370.0 3 546.5 4 484.0 5 458.5 6 654.0 7 1133.0 8 1952.5 9 2557.5 10 2646.5 11 2771.0 12 3208.0 13 3871.5 14 4686.0 15 5648.0 16 6559.0 17 7422.0 18 8307.0 19 9054.0 20 9965.5 21 10821.5 22 12052.5 23 13722.0 24 15810.5 25 18493.5 26 22151.5 27 26687.0 28 32738.5 29 44323.0 30 61788.5 31 80136.0 32 105648.0 33 135469.5 34 159751.5 35 183603.0 36 207578.0 37 242592.5 38 259796.0 39 251375.5 40 238253.5 41 223345.5 42 220036.5 43 229421.5 44 247463.0 45 283759.5 46 319791.5 47 345498.0 48 362204.0 49 354761.0 50 337076.0 51 343301.5 52 340992.5 53 316610.5 54 293508.5 55 248184.0 56 212340.0 57 171794.0 58 130985.0 59 123408.0 60 101210.5 61 67497.5 62 48395.5 63 41112.0 64 30285.5 65 13398.5 66 6737.0 67 4484.0 68 3354.5 69 2556.5 70 1945.5 71 1445.0 72 1115.0 73 825.0 74 623.0 75 476.0 76 348.5 77 253.5 78 190.5 79 137.0 80 115.0 81 96.0 82 74.0 83 62.0 84 43.0 85 31.5 86 31.5 87 28.5 88 25.5 89 24.0 90 19.5 91 11.5 92 8.5 93 5.5 94 5.0 95 5.0 96 3.0 97 2.0 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05791184341120031 2 0.13472993047169898 3 0.017228019525088793 4 0.01682157462000679 5 0.02003380048275166 6 0.05397850562008415 7 0.05939340064585407 8 0.050136945710760696 9 0.04118204667298623 10-11 0.040041378713562545 12-13 0.041260713428808556 14-15 0.01889313252332797 16-17 0.017916353638534124 18-19 0.017811464630771025 20-21 0.021587468910242543 22-23 0.025914140480470322 24-25 0.025193028552099025 26-27 0.019706022333491977 28-29 0.018512909870186743 30-31 0.016461018655821145 32-33 0.023685249065504496 34-35 0.023770471384312014 36-37 0.023606582309682173 38-39 0.021574357784272153 40-41 0.02529791755986212 42-43 0.032404147835812 44-45 0.0425456037739065 46-47 0.047698276280268674 48-49 0.04032982348491106 50-51 0.028313476533051184 52-53 0.040434712492674156 54-55 0.03705859755529945 56-57 0.03909737764369466 58-59 0.044204161209160486 60-61 0.023468915486993105 62-63 0.02611080737002613 64-65 0.02543558438255119 66-67 0.03288925949671632 68-69 0.03989060076490309 70-71 0.04147704700731994 72-73 0.045279273538732234 74-75 0.027605475730650274 76-77 0.02080080135201931 78-79 0.019915800349018173 80-81 0.024662027950298343 82-83 0.02065657896634505 84-85 0.012534236427690174 86-87 0.014540238701159417 88-89 0.018512909870186743 90-91 0.0212203573830717 92-93 0.019771577963343914 94-95 0.02365902681356372 96-97 0.024917694906720893 98-99 0.027926698316924757 100 0.029827811582630908 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7627110.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.72525959126132 #Duplication Level Percentage of deduplicated Percentage of total 1 78.07233657401927 53.655415979458 2 14.037834883653854 19.295076929567497 3 4.3201125018997795 8.907025594695474 4 1.6681827650110683 4.58585174284215 5 0.7866323050273597 2.703075468293878 6 0.38065539237324447 1.5696384393398797 7 0.21309049653090692 1.0251289783362145 8 0.12495262772497902 0.6869921421607535 9 0.0869646852070128 0.5379003509511849 >10 0.27932729986541593 3.130503188450376 >50 0.017243381723418633 0.8189644277957012 >100 0.011350504580149133 1.4503725473407267 >500 9.239376800545261E-4 0.41828645751217375 >1k 3.233544620137353E-4 0.36735577046354184 >5k 0.0 0.0 >10k+ 6.929024155118894E-5 0.8484119827924045 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23909 0.3134739108259878 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18541 0.24309338661694926 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10999 0.1442092745482889 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.3111125970387211E-5 0.0 0.0 0.0 0.0 7 1.3111125970387211E-5 0.0 0.0 0.0 0.0 8 1.3111125970387211E-5 0.0 0.0 1.3111125970387211E-5 0.0 9 1.3111125970387211E-5 0.0 0.0 1.3111125970387211E-5 0.0 10-11 1.3111125970387211E-5 0.0 0.0 2.6222251940774423E-5 0.0 12-13 1.3111125970387211E-5 0.0 0.0 3.933337791116163E-5 0.0 14-15 1.3111125970387211E-5 0.0 0.0 3.933337791116163E-5 0.0 16-17 5.2444503881548846E-5 0.0 0.0 4.588894089635524E-5 0.0 18-19 5.2444503881548846E-5 0.0 0.0 7.866675582232326E-5 0.0 20-21 5.2444503881548846E-5 0.0 0.0 1.4422238567425932E-4 0.0 22-23 5.2444503881548846E-5 0.0 0.0 3.2122258627448666E-4 0.0 24-25 5.900006686674245E-5 0.0 0.0 6.883341134453285E-4 0.0 26-27 6.555562985193606E-5 0.0 0.0 0.0014881127976389484 0.0 28-29 6.555562985193606E-5 0.0 0.0 0.004543005148739169 0.0 30-31 6.555562985193606E-5 0.0 0.0 0.017804909067785833 0.0 32-33 6.555562985193606E-5 0.0 0.0 0.04860294397222539 0.0 34-35 6.555562985193606E-5 0.0 0.0 0.09481966301784031 0.0 36-37 6.555562985193606E-5 0.0 0.0 0.17242441763656222 0.0 38-39 7.866675582232326E-5 0.0 0.0 0.2876515482273102 0.0 40-41 7.866675582232326E-5 0.0 0.0 0.4095784641889261 0.0 42-43 9.177788179271048E-5 0.0 0.0 0.5278408204418187 0.0 44-45 9.177788179271048E-5 0.0 0.0 0.6674349786485314 0.0 46-47 9.177788179271048E-5 0.0 0.0 0.8328711661428772 0.0 48-49 9.177788179271048E-5 0.0 0.0 1.0191737106190943 0.0 50-51 9.177788179271048E-5 0.0 0.0 1.2101765937556952 0.0 52-53 9.177788179271048E-5 0.0 0.0 1.3880290175439978 0.0 54-55 9.177788179271048E-5 0.0 0.0 1.5801201241361407 0.0 56-57 9.177788179271048E-5 0.0 0.0 1.8070016034907064 0.0 58-59 9.177788179271048E-5 0.0 0.0 2.068286677391568 0.0 60-61 9.177788179271048E-5 0.0 0.0 2.330246974279904 0.0 62-63 9.177788179271048E-5 0.0 0.0 2.5902996023395497 0.0 64-65 9.177788179271048E-5 0.0 0.0 2.8597004632160807 0.0 66-67 9.177788179271048E-5 0.0 0.0 3.155487465108016 0.0 68-69 9.177788179271048E-5 0.0 0.0 3.4792077208798613 0.0 70-71 9.177788179271048E-5 0.0 0.0 3.8306121191381797 0.0 72-73 9.177788179271048E-5 0.0 0.0 4.184697742657441 0.0 74-75 9.833344477790408E-5 0.0 0.0 4.520263900743532 0.0 76-77 1.0488900776309769E-4 0.0 0.0 4.900171886861472 0.0 78-79 1.0488900776309769E-4 0.0 0.0 5.306243124853319 0.0 80-81 1.0488900776309769E-4 0.0 0.0 5.734898277329158 0.0 82-83 1.0488900776309769E-4 0.0 0.0 6.1701942151089995 0.0 84-85 1.1800013373348489E-4 0.0 0.0 6.585010574123095 0.0 86-87 1.1800013373348489E-4 0.0 0.0 7.025826295936469 0.0 88 1.1800013373348489E-4 0.0 0.0 7.377053693994187 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15930 0.0 43.57576 1 GTATCAA 27400 0.0 38.645653 1 GTACTAG 8725 0.0 36.786434 1 GGGCACC 7815 0.0 35.961643 94 GCATCGT 7995 0.0 35.747093 8 CAACGCA 30240 0.0 32.92454 5 TACCCCG 540 0.0 32.20947 5 ATCAACG 30980 0.0 32.198788 3 TAGGCAT 10275 0.0 32.025196 5 TATCAAC 33750 0.0 31.151236 2 CTAGGCA 11320 0.0 30.023926 4 AACGCAG 35050 0.0 29.358273 6 CTGTGCG 10680 0.0 29.049 9 TACCTGG 3705 0.0 28.579865 2 TAGTACT 11535 0.0 27.834196 4 TCAACGC 36975 0.0 27.41045 4 GTGGTAT 5590 0.0 27.36152 1 ACGCAGA 36615 0.0 27.179243 7 CGCAGAG 36715 0.0 27.12958 8 ACTGTGC 12110 0.0 26.899532 8 >>END_MODULE