FastQCFastQC Report
Mon 6 Jun 2016
ERR1211256_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211256_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7627110
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218340.2862683244374344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179820.23576426719950283No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106260.13931882456133451No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA160100.043.7872471
GTATCAA275050.038.1714251
GGGCACC77700.037.7512194
GTACTAG83900.037.3787841
CAACGCA300350.032.89795
TAGGCAT100850.032.8450365
ATCAACG306200.032.296583
TATCAAC343600.030.3919432
CTGTGCG110300.029.4774539
GCATCGT98600.029.3060688
AACGCAG346550.029.2713856
CTAGGCA115550.029.2465694
TCAACGC363300.027.6474534
TAGTACT118000.027.4443684
GTGGTAT53450.027.3573471
ACTGTGC125000.026.9881928
ACGCAGA368700.026.7737647
CGCAGAG369450.026.7195878
CGACGAG54100.026.703824-25
TACCTGG38650.026.0214732
Produced by FastQC (version 0.11.5)