FastQCFastQC Report
Mon 6 Jun 2016
ERR1211255_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211255_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4825782
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162700.3371474301988776No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128740.2667754158807837No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81020.1678898881051817No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82850.048.400751
GTATCAA144550.040.863041
TACCTGG37600.039.804372
GTACTAG30050.034.457461
CAACGCA172250.033.0520825
ATCAACG172750.032.9288643
TATCAAC184350.031.759142
GCATCGT29600.031.126398
AACGCAG189750.030.6228836
GTACCTG49950.030.5291391
TCAACGC187900.030.4490134
ACCTGGG47650.029.9930133
GTGGTAT32600.029.3078171
GTACATG192750.027.5923751
ACGCAGA208600.027.2020957
TAGGCAT38900.026.9506455
TACATGG196350.026.7260912
CGCAGAG213150.026.5966158
CTGTGCG40450.026.4960219
TAGTACT40650.026.2527454
Produced by FastQC (version 0.11.5)