##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211255_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4825782 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.441460264885567 31.0 31.0 34.0 29.0 34.0 2 31.44667496376753 31.0 31.0 34.0 28.0 34.0 3 31.509161209520034 33.0 31.0 34.0 28.0 34.0 4 35.101745375153705 37.0 35.0 37.0 32.0 37.0 5 35.160873615923805 37.0 35.0 37.0 32.0 37.0 6 35.225577740561015 37.0 35.0 37.0 32.0 37.0 7 35.14272443305562 37.0 35.0 37.0 32.0 37.0 8 35.14728887463213 37.0 35.0 37.0 32.0 37.0 9 36.71283949420011 39.0 37.0 39.0 32.0 39.0 10-11 36.6615485324451 39.0 37.0 39.0 32.0 39.0 12-13 36.554720457741354 39.0 37.0 39.0 32.0 39.0 14-15 37.75194735278137 40.0 37.0 41.0 32.0 41.0 16-17 37.7237851813447 40.0 37.0 41.0 32.0 41.0 18-19 37.730798759662164 40.0 37.0 41.0 32.0 41.0 20-21 37.69473631423881 40.0 37.0 41.0 32.0 41.0 22-23 37.58568766678644 40.0 37.0 41.0 32.0 41.0 24-25 37.49049221452606 40.0 37.0 41.0 31.5 41.0 26-27 37.34309050843987 40.0 37.0 41.0 31.0 41.0 28-29 37.21592448643557 40.0 36.0 41.0 31.0 41.0 30-31 37.05485173180222 39.5 36.0 41.0 30.5 41.0 32-33 36.85941273352174 39.0 36.0 41.0 30.0 41.0 34-35 36.7056897721447 39.0 35.0 41.0 30.0 41.0 36-37 36.59192282204211 39.0 35.0 41.0 30.0 41.0 38-39 36.44965385920872 39.0 35.0 41.0 29.5 41.0 40-41 36.278352296063105 39.0 35.0 40.0 29.0 41.0 42-43 36.06931280774805 39.0 35.0 40.0 28.0 41.0 44-45 35.85147443460977 38.0 35.0 40.0 27.5 41.0 46-47 35.614253814200474 38.0 34.0 40.0 26.5 41.0 48-49 35.41126671283535 38.0 34.0 40.0 26.0 41.0 50-51 34.724119945741435 37.5 33.5 39.5 25.0 40.5 52-53 34.82815324024168 38.0 33.5 39.5 25.5 40.5 54-55 35.31258467539561 38.0 34.0 40.0 26.0 41.0 56-57 35.27283857828638 38.0 34.0 40.0 26.0 41.0 58-59 35.05567646860136 38.0 34.0 40.0 26.0 41.0 60-61 34.82598633755109 37.0 34.0 40.0 26.0 41.0 62-63 34.514689432717844 37.0 33.0 40.0 26.0 41.0 64-65 34.262441403279304 36.0 33.0 39.5 25.0 41.0 66-67 34.08201613748818 36.0 33.0 39.0 25.0 41.0 68-69 33.78114780153766 35.0 33.0 39.0 25.0 41.0 70-71 33.46631737612681 35.0 33.0 38.5 24.0 40.5 72-73 33.12561622137096 35.0 32.0 37.5 24.0 40.0 74-75 32.795609395534235 35.0 32.0 37.0 23.5 39.5 76-77 32.47150606886096 35.0 32.0 36.5 23.0 39.0 78-79 32.14473177611421 35.0 31.0 36.0 22.5 39.0 80-81 31.855570143864767 35.0 31.0 36.0 21.5 37.5 82-83 31.57308877607816 35.0 31.0 35.0 20.5 37.0 84-85 31.3365005920284 34.5 31.0 35.0 20.0 37.0 86-87 31.119620405563285 34.0 31.0 35.0 20.0 36.0 88-89 30.91006328093561 34.0 30.0 35.0 19.5 36.0 90-91 30.691882890690046 34.0 30.0 35.0 19.0 36.0 92-93 30.503650910878278 34.0 30.0 35.0 18.5 35.0 94-95 30.32850261366966 34.0 29.0 35.0 18.0 35.0 96-97 30.151661948260404 34.0 29.0 35.0 18.0 35.0 98-99 29.9218205463902 34.0 29.0 35.0 17.0 35.0 100 28.78876356205067 33.0 26.0 35.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.44244170726354426 1101 2 -0.3462996441245245 1101 3 -0.3952901788562535 1101 4 -0.33482248177232066 1101 5 -0.2701659558145053 1101 6 -0.24755233348872707 1101 7 -0.24943959235060476 1101 8 -0.20275118990380037 1101 9 -0.4687800989851638 1101 10-11 -0.48100551156139204 1101 12-13 -0.4868978181270194 1101 14-15 -0.6209138890092092 1101 16-17 -0.5898061953762195 1101 18-19 -0.5558935126582085 1101 20-21 -0.6448114486024608 1101 22-23 -0.6764855605344806 1101 24-25 -0.8276216052696981 1101 26-27 -0.7635696991351537 1101 28-29 -0.8507615298087714 1101 30-31 -0.923663951884798 1101 32-33 -0.9168977650963157 1101 34-35 -0.8471342267615043 1101 36-37 -0.820849422376142 1101 38-39 -0.9205161970475046 1101 40-41 -0.9065927658256001 1101 42-43 -0.9157307822558707 1101 44-45 -0.9171077556654836 1101 46-47 -1.0900122035924795 1101 48-49 -1.130889668646752 1101 50-51 -1.1036526759496184 1101 52-53 -0.9781577077181325 1101 54-55 -0.9423619868906314 1101 56-57 -0.789734460561796 1101 58-59 -0.7940591807165589 1101 60-61 -0.8362489763251872 1101 62-63 -1.001661930451128 1101 64-65 -0.8009391292664461 1101 66-67 -0.8913467466344684 1101 68-69 -0.8844231233274797 1101 70-71 -0.9495325401585504 1101 72-73 -0.8943661137108521 1101 74-75 -0.9887242227985666 1101 76-77 -0.9350609330817399 1101 78-79 -1.0335622409981795 1101 80-81 -1.1462643070963416 1101 82-83 -1.0391946066643918 1101 84-85 -1.1277224908705392 1101 86-87 -1.2033049068178236 1101 88-89 -1.2229790735093715 1101 90-91 -1.219488020953996 1101 92-93 -1.3234917846595344 1101 94-95 -1.3988684344803453 1101 96-97 -1.4118629963069118 1101 98-99 -1.5579489117498646 1101 100 -1.8605142030665505 1102 1 -0.501790609738272 1102 2 -0.43343325555348144 1102 3 -0.4937607077865813 1102 4 -0.26003885517890524 1102 5 -0.3359435701701372 1102 6 -0.32900146582247913 1102 7 -0.32672414834346597 1102 8 -0.37531422118121327 1102 9 -0.5122070833023429 1102 10-11 -0.6252451223679643 1102 12-13 -0.6104154150490047 1102 14-15 -0.7224656184822038 1102 16-17 -0.7982724646505588 1102 18-19 -0.704419251426522 1102 20-21 -0.8396705200457362 1102 22-23 -0.8505950762680001 1102 24-25 -0.8868777598252109 1102 26-27 -0.8858874710080826 1102 28-29 -0.9822630739066298 1102 30-31 -0.9915570198158932 1102 32-33 -1.0252017954638646 1102 34-35 -1.0786767324662776 1102 36-37 -1.0644528609126667 1102 38-39 -1.0851164312956598 1102 40-41 -1.1330181704929174 1102 42-43 -1.0506316843548902 1102 44-45 -1.2228477189196383 1102 46-47 -1.2536118795426106 1102 48-49 -1.182618181524255 1102 50-51 -1.242390752314428 1102 52-53 -1.0648396222414718 1102 54-55 -0.9141351822646584 1102 56-57 -0.9206321693452892 1102 58-59 -1.003736631928028 1102 60-61 -0.9736804619202601 1102 62-63 -0.9308876145308105 1102 64-65 -0.9645244307978587 1102 66-67 -0.9723870309977798 1102 68-69 -0.9698807708035275 1102 70-71 -1.0840572659143888 1102 72-73 -1.090432279708832 1102 74-75 -1.1740740950527808 1102 76-77 -1.3274975418952124 1102 78-79 -1.2426178614397863 1102 80-81 -1.265031021472911 1102 82-83 -1.2537962289766504 1102 84-85 -1.3114999553371973 1102 86-87 -1.2790252572200664 1102 88-89 -1.3898950979357885 1102 90-91 -1.3653613777165354 1102 92-93 -1.3612938449636225 1102 94-95 -1.4813317862963302 1102 96-97 -1.4786468748367092 1102 98-99 -1.4299728584341587 1102 100 -1.6142978573115272 1103 1 -0.47623079789858025 1103 2 -0.4350509900927868 1103 3 -0.52314026376947 1103 4 -0.4353754533982155 1103 5 -0.4237316837254923 1103 6 -0.4011310928104592 1103 7 -0.4086090983716346 1103 8 -0.45691131846735544 1103 9 -0.5481477975603823 1103 10-11 -0.6529059185703545 1103 12-13 -0.6318141100456103 1103 14-15 -0.7692845635785517 1103 16-17 -0.7778952796204379 1103 18-19 -0.7505568350305012 1103 20-21 -0.7332903023422048 1103 22-23 -0.7604784054212175 1103 24-25 -0.8088462201494337 1103 26-27 -0.9124955660999774 1103 28-29 -0.852420808479998 1103 30-31 -0.9261228433493827 1103 32-33 -0.9743601508793986 1103 34-35 -0.9601193271351249 1103 36-37 -0.9048639048344853 1103 38-39 -0.9711130926722547 1103 40-41 -1.007397488017915 1103 42-43 -1.0872736670811705 1103 44-45 -1.2268610770327157 1103 46-47 -1.138946128312952 1103 48-49 -1.133007788720228 1103 50-51 -1.11308581555474 1103 52-53 -1.0014006676749432 1103 54-55 -0.8355485626480075 1103 56-57 -0.7642879704935481 1103 58-59 -0.7700458743430474 1103 60-61 -0.7891202006300233 1103 62-63 -0.7844300236283175 1103 64-65 -0.8428240652759698 1103 66-67 -0.8668842638216745 1103 68-69 -0.7708172662730348 1103 70-71 -0.7608008987692827 1103 72-73 -0.8126635121145824 1103 74-75 -0.8460524556377322 1103 76-77 -0.8517244912139645 1103 78-79 -0.9582155199536047 1103 80-81 -0.9473237770795429 1103 82-83 -0.7859566404527776 1103 84-85 -0.9886786141648471 1103 86-87 -1.0893052568197952 1103 88-89 -1.0589534937188319 1103 90-91 -1.1125830239020793 1103 92-93 -1.0929846168134958 1103 94-95 -1.141110550599013 1103 96-97 -1.3101979838149482 1103 98-99 -1.3599085535793698 1103 100 -1.4239779064338514 1104 1 -0.5061124524213447 1104 2 -0.45614220289263585 1104 3 -0.5041886961729212 1104 4 -0.343708364597731 1104 5 -0.3767162949152265 1104 6 -0.4409992869970196 1104 7 -0.4779709487728283 1104 8 -0.506895141882417 1104 9 -0.6496811960586726 1104 10-11 -0.6112738043386514 1104 12-13 -0.6069440178203109 1104 14-15 -0.7436665127611448 1104 16-17 -0.8140834831377219 1104 18-19 -0.9005892663732951 1104 20-21 -0.7479439483172214 1104 22-23 -0.8350474589054713 1104 24-25 -0.8385253807264306 1104 26-27 -0.8148368870390428 1104 28-29 -0.8624477896061364 1104 30-31 -0.7970146995437162 1104 32-33 -0.8502317269311277 1104 34-35 -0.9516229198587425 1104 36-37 -1.0000100538170784 1104 38-39 -0.9927228786306017 1104 40-41 -0.9516916146042718 1104 42-43 -0.9758421946179467 1104 44-45 -1.0122474086939803 1104 46-47 -1.0292270859917991 1104 48-49 -1.0364806888754785 1104 50-51 -1.0322302514036394 1104 52-53 -1.0332793018041642 1104 54-55 -0.7099572886648176 1104 56-57 -0.8915998046035085 1104 58-59 -0.9117846789893065 1104 60-61 -0.8513529143542939 1104 62-63 -0.9048297551720523 1104 64-65 -0.8365530849644998 1104 66-67 -0.9082829276274254 1104 68-69 -0.9618910069019293 1104 70-71 -0.8211706212887364 1104 72-73 -0.9017228040929695 1104 74-75 -0.8668937235234395 1104 76-77 -0.8045019180821491 1104 78-79 -0.9543194853640671 1104 80-81 -0.9650736964571962 1104 82-83 -0.9366290475140211 1104 84-85 -0.9149917421931306 1104 86-87 -1.0219325947897246 1104 88-89 -1.1283199823805248 1104 90-91 -1.2015926500124259 1104 92-93 -1.1655670095180248 1104 94-95 -1.320298175428153 1104 96-97 -1.4792764677687593 1104 98-99 -1.3145608278800474 1104 100 -1.2254866231324435 1105 1 -0.5434432765315442 1105 2 -0.4960276633953562 1105 3 -0.4883858520461466 1105 4 -0.2720134947125956 1105 5 -0.3944139805246891 1105 6 -0.4226135062005483 1105 7 -0.467505414321586 1105 8 -0.5087473796987396 1105 9 -0.659908425829336 1105 10-11 -0.6265094935423505 1105 12-13 -0.6070315239120276 1105 14-15 -0.7831139830535179 1105 16-17 -0.6846507095777454 1105 18-19 -0.8055833713336042 1105 20-21 -0.7177291880369197 1105 22-23 -0.7403093692865639 1105 24-25 -0.7537004337766788 1105 26-27 -0.7753282186743604 1105 28-29 -0.8214200502261448 1105 30-31 -0.8099554099521882 1105 32-33 -0.9321720889499048 1105 34-35 -0.8726636651061952 1105 36-37 -0.9298433478160888 1105 38-39 -0.9631453343386198 1105 40-41 -1.0372736450521103 1105 42-43 -1.0369350603939225 1105 44-45 -1.1545495800506274 1105 46-47 -1.0757034971488437 1105 48-49 -1.0745927845365415 1105 50-51 -1.0009146665329425 1105 52-53 -0.9305255673424924 1105 54-55 -0.7768325804794927 1105 56-57 -0.7179100461366659 1105 58-59 -0.6627924804664431 1105 60-61 -0.7085702768297608 1105 62-63 -0.7942577370966575 1105 64-65 -0.7252315948374175 1105 66-67 -0.6984695255560922 1105 68-69 -0.6862111361636991 1105 70-71 -0.7059049205095107 1105 72-73 -0.7815197777001543 1105 74-75 -0.7436738569331922 1105 76-77 -0.6426959261880825 1105 78-79 -0.6656687138494704 1105 80-81 -0.814808240878925 1105 82-83 -0.879199790544444 1105 84-85 -0.8935155353702129 1105 86-87 -0.9832290539857276 1105 88-89 -0.9422941892831354 1105 90-91 -1.0723267408123824 1105 92-93 -1.0054703237154676 1105 94-95 -1.1340814831686359 1105 96-97 -1.1242190192277555 1105 98-99 -1.0865361208185682 1105 100 -1.160800567200063 1106 1 -0.6031865394534535 1106 2 -0.5247634575240809 1106 3 -0.640380940809365 1106 4 -0.4673302145952931 1106 5 -0.43859219447461584 1106 6 -0.42227926082133393 1106 7 -0.4450776859684211 1106 8 -0.5083377558398823 1106 9 -0.6902554476078961 1106 10-11 -0.614238661194463 1106 12-13 -0.7068180105742101 1106 14-15 -0.874583997469017 1106 16-17 -0.8707145187254994 1106 18-19 -0.9406603927099795 1106 20-21 -0.8338248205622776 1106 22-23 -0.9408997750226149 1106 24-25 -0.911287416874444 1106 26-27 -1.0307704057834783 1106 28-29 -1.142670483083272 1106 30-31 -1.1195556434737028 1106 32-33 -1.1359391158228433 1106 34-35 -1.1269870888675655 1106 36-37 -1.2232144429009821 1106 38-39 -1.15659436267714 1106 40-41 -1.2501785166519497 1106 42-43 -1.143603880490538 1106 44-45 -1.1516668338051943 1106 46-47 -1.1345887101423742 1106 48-49 -1.2336733997969418 1106 50-51 -1.0010719242537434 1106 52-53 -0.9621387958103682 1106 54-55 -1.0441766248081095 1106 56-57 -1.0647574171098313 1106 58-59 -1.0078805733731997 1106 60-61 -0.96864624214858 1106 62-63 -1.014461446173165 1106 64-65 -0.9688542086848457 1106 66-67 -0.9262825403841504 1106 68-69 -0.917832408375375 1106 70-71 -0.9826891582117483 1106 72-73 -1.0081935656938796 1106 74-75 -0.9620050937498661 1106 76-77 -0.891848892141546 1106 78-79 -0.8183041655625978 1106 80-81 -0.7782418621597351 1106 82-83 -0.8400046252897724 1106 84-85 -0.9840662358715306 1106 86-87 -0.9304014377390892 1106 88-89 -0.9596639963572322 1106 90-91 -1.0458053033498338 1106 92-93 -1.0959306965151647 1106 94-95 -1.1825839176658768 1106 96-97 -1.1774785693396446 1106 98-99 -0.999388075713906 1106 100 -1.305624127173889 1107 1 -0.5596534097552279 1107 2 -0.6000834833071487 1107 3 -0.5812759103846368 1107 4 -0.4680108041438231 1107 5 -0.4172913273589671 1107 6 -0.48082488092106246 1107 7 -0.4957607356577327 1107 8 -0.514688624081856 1107 9 -0.7830403487062867 1107 10-11 -0.6955650861099727 1107 12-13 -0.7740245118272 1107 14-15 -0.8618799638656682 1107 16-17 -0.8528247257211561 1107 18-19 -0.8618843384359209 1107 20-21 -0.8961308498239191 1107 22-23 -0.8825800383307936 1107 24-25 -0.8934144694123844 1107 26-27 -1.0358511151971825 1107 28-29 -1.0326757857240594 1107 30-31 -0.9648619912692382 1107 32-33 -1.1264559894245636 1107 34-35 -1.0922331296217251 1107 36-37 -1.1024020915715411 1107 38-39 -1.1079341859738179 1107 40-41 -1.147753889832579 1107 42-43 -1.0689669142768636 1107 44-45 -1.154473320909915 1107 46-47 -0.9449139555735329 1107 48-49 -1.1287634873858607 1107 50-51 -1.1775763299805462 1107 52-53 -0.9724292238274401 1107 54-55 -0.8762448463891346 1107 56-57 -0.9036017951249846 1107 58-59 -0.856874440014991 1107 60-61 -0.9377208152400556 1107 62-63 -0.9322531991094181 1107 64-65 -0.8573938009456583 1107 66-67 -0.8563714403870364 1107 68-69 -0.8104552415248705 1107 70-71 -0.7438516517946354 1107 72-73 -0.7058894907127424 1107 74-75 -0.6988950875175064 1107 76-77 -0.7605239838188425 1107 78-79 -0.6637326805900088 1107 80-81 -0.7555108529645409 1107 82-83 -0.8434247149772212 1107 84-85 -0.7514515271146536 1107 86-87 -0.7578433102441196 1107 88-89 -0.7322280568423096 1107 90-91 -0.7913876957369546 1107 92-93 -0.963639120827601 1107 94-95 -0.8427111958649185 1107 96-97 -0.8085817685897077 1107 98-99 -0.8912848956312054 1107 100 -0.9440002287537936 1108 1 -0.6116043816399568 1108 2 -0.6164520580777761 1108 3 -0.6239123530481194 1108 4 -0.3850605571236798 1108 5 -0.41636675910981324 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2311 48-49 1.3963030195838328 2311 50-51 1.4385359852285902 2311 52-53 1.2944039768826414 2311 54-55 1.253764162699781 2311 56-57 1.2316145243770933 2311 58-59 1.1527947293875584 2311 60-61 1.1372504690809677 2311 62-63 1.167420221417359 2311 64-65 1.1502722399842398 2311 66-67 1.125099502450233 2311 68-69 1.1696074768124873 2311 70-71 1.1272300755936229 2311 72-73 1.12919365646232 2311 74-75 1.0695319680055917 2311 76-77 1.1118090059189072 2311 78-79 1.1486653969641836 2311 80-81 1.1273408974722905 2311 82-83 1.1781535418211 2311 84-85 1.239424394543022 2311 86-87 1.1721984534085799 2311 88-89 1.282911663229715 2311 90-91 1.2882300483669091 2311 92-93 1.3452562932800483 2311 94-95 1.3693495001790659 2311 96-97 1.424241963073758 2311 98-99 1.4779676921060414 2311 100 1.5156144077845894 2312 1 0.6084026075978031 2312 2 0.634841351586676 2312 3 0.6475136152104284 2312 4 0.2684105262116532 2312 5 0.43865533573129056 2312 6 0.5298197448894939 2312 7 0.439852588821509 2312 8 0.5519364497669343 2312 9 0.694172274337518 2312 10-11 0.6470432360548415 2312 12-13 0.7731472852153729 2312 14-15 1.0209966517657776 2312 16-17 1.0505795249577332 2312 18-19 0.9713979837394575 2312 20-21 1.008894212972777 2312 22-23 1.0504475889553433 2312 24-25 0.9820524699243549 2312 26-27 1.1392708109779335 2312 28-29 1.181457471641643 2312 30-31 1.171464658962094 2312 32-33 1.1170423872214954 2312 34-35 1.133948346230234 2312 36-37 1.2156740547184484 2312 38-39 1.317487054816226 2312 40-41 1.2917173256711436 2312 42-43 1.306626902945716 2312 44-45 1.2930392050555213 2312 46-47 1.289439472857488 2312 48-49 1.2352730331106514 2312 50-51 1.3105692318430258 2312 52-53 1.1814531618664361 2312 54-55 1.10456455807374 2312 56-57 1.0334417821026065 2312 58-59 1.0136951565121635 2312 60-61 0.9880993564772069 2312 62-63 0.9703591659176496 2312 64-65 1.0387718713429805 2312 66-67 0.9667200205042263 2312 68-69 0.9972305004043847 2312 70-71 0.9338973975234737 2312 72-73 1.0007363949682428 2312 74-75 1.0063247024678645 2312 76-77 0.9174860780884089 2312 78-79 0.9872069104017882 2312 80-81 1.0311195435852163 2312 82-83 1.1332445005010534 2312 84-85 1.1361946220842754 2312 86-87 1.0967993764827177 2312 88-89 1.0768601135673244 2312 90-91 1.165445048047875 2312 92-93 1.273705629846777 2312 94-95 1.3286957054538497 2312 96-97 1.3156514341674814 2312 98-99 0.958560646695986 2312 100 1.2492920503960292 2313 1 0.6441304729716855 2313 2 0.5939368143260708 2313 3 0.6318821386211155 2313 4 0.5285641347515195 2313 5 0.43421089609684316 2313 6 0.4797029460740987 2313 7 0.5518419757978776 2313 8 0.5829566482780209 2313 9 0.7447003581815821 2313 10-11 0.7540953538435602 2313 12-13 0.8290666420847543 2313 14-15 0.968945451484494 2313 16-17 1.0292055251219878 2313 18-19 1.0128447582679598 2313 20-21 1.0291832873352504 2313 22-23 1.0295987659201558 2313 24-25 1.0505875087873449 2313 26-27 1.0512151703455075 2313 28-29 1.1222860590084949 2313 30-31 1.0778770227534196 2313 32-33 1.140073747577162 2313 34-35 1.1274609193888168 2313 36-37 1.2275321768056173 2313 38-39 1.2079095218914802 2313 40-41 1.2517770279874867 2313 42-43 1.3681633322593498 2313 44-45 1.3557302296804323 2313 46-47 1.3537299726633378 2313 48-49 1.2753501301893166 2313 50-51 1.3223295305632448 2313 52-53 1.2145780207981645 2313 54-55 1.0918877139289052 2313 56-57 1.0279885051761397 2313 58-59 0.9778424716485503 2313 60-61 1.0338789034930898 2313 62-63 1.0199973096474153 2313 64-65 1.0547844051197046 2313 66-67 0.9458134474112967 2313 68-69 1.0023244102354099 2313 70-71 1.0519701864057325 2313 72-73 1.0866100995301338 2313 74-75 1.0660518504303127 2313 76-77 0.8331791707726524 2313 78-79 1.055854367974284 2313 80-81 1.111024970575496 2313 82-83 1.1518662185631676 2313 84-85 1.1585097284051074 2313 86-87 1.1112935737593226 2313 88-89 1.2606504690130684 2313 90-91 1.3180179799675003 2313 92-93 1.3281507969936648 2313 94-95 1.3147479241323055 2313 96-97 1.3561665418351119 2313 98-99 1.4448855322270155 2313 100 1.5222779562554365 2314 1 0.5274462186829361 2314 2 0.4870967211430788 2314 3 0.5562436254975296 2314 4 0.44869929248658025 2314 5 0.465465710119517 2314 6 0.4499928591802558 2314 7 0.524597021835838 2314 8 0.4729855265253562 2314 9 0.6463606218545834 2314 10-11 0.6543990347601252 2314 12-13 0.7262793965654737 2314 14-15 0.8112501405486867 2314 16-17 0.7651859133530436 2314 18-19 0.8010921242467433 2314 20-21 0.8495142447692743 2314 22-23 0.871577455846456 2314 24-25 0.829237804047402 2314 26-27 0.8467196092571854 2314 28-29 0.8740037376308223 2314 30-31 0.8872644552982223 2314 32-33 0.9168436590815858 2314 34-35 0.9091863004474234 2314 36-37 0.8813260388825412 2314 38-39 0.8385802933835151 2314 40-41 0.9198689201722203 2314 42-43 1.0807912056567304 2314 44-45 1.097797250298001 2314 46-47 1.0916413286925533 2314 48-49 1.0032577998843522 2314 50-51 1.0261528950910517 2314 52-53 0.9823052250022357 2314 54-55 0.9206225711040688 2314 56-57 0.8570124981473768 2314 58-59 0.785217024400815 2314 60-61 0.8257561308986823 2314 62-63 0.891359145135624 2314 64-65 0.8543674842569331 2314 66-67 0.856783353733654 2314 68-69 0.8127136305933931 2314 70-71 0.8293944516710425 2314 72-73 0.8734994694614215 2314 74-75 0.818360873587082 2314 76-77 0.8793520963558095 2314 78-79 0.7650618414574168 2314 80-81 0.798839339505701 2314 82-83 0.7986722985413088 2314 84-85 0.8738358099849748 2314 86-87 0.8688545631572815 2314 88-89 0.993811753788858 2314 90-91 1.06141125647083 2314 92-93 1.0667039049384819 2314 94-95 1.1631429554720363 2314 96-97 1.1074965664970406 2314 98-99 1.2076438994989616 2314 100 1.2387197133531025 2315 1 0.4129241102325132 2315 2 0.467829722865833 2315 3 0.5078689761207293 2315 4 0.22376877013815744 2315 5 0.2960310244055364 2315 6 0.3641812984519319 2315 7 0.4133821672345448 2315 8 0.40468598065186967 2315 9 0.6048620984568629 2315 10-11 0.4507764920633548 2315 12-13 0.5070915513460932 2315 14-15 0.7269856596415778 2315 16-17 0.6927722170949338 2315 18-19 0.652461400096989 2315 20-21 0.7129417677417464 2315 22-23 0.500493083950893 2315 24-25 0.6838286078825035 2315 26-27 0.76885655654592 2315 28-29 0.8048955601185881 2315 30-31 0.8025519060315816 2315 32-33 0.8398378797173152 2315 34-35 0.8303050803228089 2315 36-37 0.8836555767510248 2315 38-39 0.9330693407647743 2315 40-41 0.9452954320794476 2315 42-43 1.0051117362807176 2315 44-45 1.076756329505173 2315 46-47 1.1045331458867835 2315 48-49 1.1233086825225556 2315 50-51 0.9997117594983251 2315 52-53 1.033877728497437 2315 54-55 0.9582556520534027 2315 56-57 0.9333977869420735 2315 58-59 0.8841644048948467 2315 60-61 0.9357317835768839 2315 62-63 0.8750779801814161 2315 64-65 0.8990689053359091 2315 66-67 0.8652328507595684 2315 68-69 0.9227070313193124 2315 70-71 0.8172152325616153 2315 72-73 0.6245344438495337 2315 74-75 0.8511301072066715 2315 76-77 0.8464338938286815 2315 78-79 0.8712979937159631 2315 80-81 0.6568190499728495 2315 82-83 0.580403646328346 2315 84-85 0.8469781025680874 2315 86-87 1.01654153817827 2315 88-89 1.0805513741953199 2315 90-91 1.1401428312917155 2315 92-93 1.1880767156940024 2315 94-95 1.164517098433958 2315 96-97 1.0425814718633326 2315 98-99 0.5102766246050834 2315 100 0.9850711146989539 2316 1 0.33029314790531217 2316 2 0.25832174442892253 2316 3 0.30600936484765384 2316 4 0.2225152349799444 2316 5 0.1422698781212759 2316 6 0.21668387081511753 2316 7 0.17180224335416483 2316 8 0.18710041687454293 2316 9 0.23553555417497307 2316 10-11 0.2349840419591942 2316 12-13 0.15773952481811193 2316 14-15 0.0803343132762322 2316 16-17 0.23650137998581755 2316 18-19 0.3458311796023068 2316 20-21 0.37035201598743583 2316 22-23 0.37940321449354286 2316 24-25 0.3806562713765089 2316 26-27 0.48642347778002915 2316 28-29 0.5079200225648108 2316 30-31 0.3812248893546837 2316 32-33 0.5526731124356203 2316 34-35 0.5495760794989408 2316 36-37 0.5566038131796276 2316 38-39 0.6727711603209627 2316 40-41 0.6285391449037263 2316 42-43 0.6050417476007866 2316 44-45 0.5884838082357433 2316 46-47 0.40421699649353826 2316 48-49 0.32985232071511206 2316 50-51 0.41270094269024327 2316 52-53 0.6599406722306398 2316 54-55 0.2836848548410913 2316 56-57 0.22427220048240315 2316 58-59 0.1756677404591258 2316 60-61 0.3039935044823636 2316 62-63 0.40078099092576025 2316 64-65 0.4018816869801114 2316 66-67 0.3807353565548084 2316 68-69 0.28871507100401317 2316 70-71 0.061833495271805816 2316 72-73 0.033549555653351604 2316 74-75 0.2908717795958182 2316 76-77 0.31951252253596607 2316 78-79 0.392066930033117 2316 80-81 0.49583747962173774 2316 82-83 0.6032470492291431 2316 84-85 0.7032840175568573 2316 86-87 0.7227245926516908 2316 88-89 0.6636945667783323 2316 90-91 0.5829489156902063 2316 92-93 0.5802828927248243 2316 94-95 0.7361804855079406 2316 96-97 0.6854527348138539 2316 98-99 0.46106870845361314 2316 100 0.3771264366218219 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 18.0 1 278.0 2 118.0 3 87.0 4 85.0 5 62.0 6 101.0 7 666.0 8 2098.0 9 3340.0 10 4491.0 11 5858.0 12 7910.0 13 9823.0 14 11430.0 15 13485.0 16 16160.0 17 20356.0 18 24950.0 19 30157.0 20 36355.0 21 44369.0 22 48837.0 23 50299.0 24 50324.0 25 51731.0 26 56327.0 27 64485.0 28 76478.0 29 90205.0 30 110057.0 31 136941.0 32 173408.0 33 228142.0 34 323075.0 35 449490.0 36 674478.0 37 960057.0 38 832577.0 39 216454.0 40 220.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.862869696977235 19.05996760525183 12.663850164752091 23.41331253301884 2 16.178099399394043 19.542808269629873 40.73428466971237 23.544807661263718 3 19.711725588426578 25.52471654840035 28.64412456326298 26.119433299910096 4 12.894723697815685 15.753921926039624 37.72087356054076 33.63048081560393 5 13.572549109827065 37.317292209559305 34.032505473228284 15.07765320738535 6 31.119925295742362 36.1583990665285 18.16798755039614 14.553688087332992 7 28.129190155380577 31.78469026205557 21.99343943607597 18.092680146487883 8 26.725112870057988 35.020992161862 19.764787444383884 18.48910752369613 9 26.851514408994287 15.819047062291583 20.775760168926887 36.55367835978724 10-11 25.983858750384027 25.754480697624377 27.226096899737435 21.035563652254165 12-13 25.982964736241147 23.38758293123621 27.78629674560457 22.84315558691807 14-15 23.366584499901396 24.731906187087993 25.749804116877222 26.151705196133385 16-17 22.464246002837314 27.471225299146006 26.642663137812868 23.421865560203813 18-19 22.043328933655502 26.963624367341215 28.897147539328966 22.095899159674314 20-21 23.183952223690095 26.305012955929115 28.52681697009089 21.984217850289898 22-23 23.22451100944481 26.02548497468447 28.295908009097115 22.454096006773607 24-25 22.712900538670194 25.935014267329564 28.84447377524505 22.50761141875519 26-27 22.655349802021536 26.187945910101433 28.274634455662078 22.88206983221495 28-29 22.253166776443553 26.71202199903916 28.51808665727933 22.51672456723796 30-31 23.104525168732305 26.28473699978104 28.500549582701602 22.11018824878506 32-33 23.10043980831616 26.409111583885103 28.001547448756302 22.488901159042435 34-35 23.165763523748655 26.424891944629803 28.311869763367888 22.097474768253655 36-37 22.91243500162496 26.267147052191042 28.078220238371593 22.742197707812405 38-39 22.913297153043167 26.478705882925958 28.27089765138315 22.33709931264772 40-41 22.857043071796827 25.82495240061335 28.632787676266965 22.685216851322853 42-43 22.709331403109914 26.083968035234868 28.109293097129708 23.097407464525514 44-45 22.591808685521436 26.631909102404904 28.212998223425494 22.563283988648166 46-47 22.70447473676769 26.33956734723155 27.691309234136973 23.264648681863783 48-49 23.0393781209444 26.358207120477044 27.690162471272306 22.912252287306252 50-51 22.90177077481075 26.416281473425425 27.629322794682547 23.05262495708128 52-53 22.70375455360143 26.79490100289619 27.368271796703475 23.133072646798905 54-55 22.535054921432575 26.649866202273476 27.574282147057062 23.240796729236894 56-57 23.06117727685154 26.432157814360167 27.211656640748327 23.295008268039968 58-59 22.5486171878061 26.644770870506125 27.72772343217123 23.078888509516545 60-61 22.802302602568506 26.92373818181366 27.359909068194604 22.914050147423232 62-63 22.94151115274961 26.62070080493803 27.150061300482214 23.28772674183014 64-65 22.51649143374015 26.50895634190091 27.344917512777155 23.62963471158179 66-67 22.650521144851083 26.347210250209844 27.289206243228982 23.713062361710087 68-69 22.323641190644224 26.47994861518781 27.48209548789815 23.714314706269814 70-71 22.415956888200743 26.196282569186945 27.58484121740749 23.80291932520482 72-73 22.662234377502298 26.406285226514747 27.470542535619757 23.460937860363195 74-75 22.821094618439332 26.544340663110283 27.21824569956226 23.41631901888812 76-77 22.64313818798022 26.306673287946065 27.165860976345783 23.884327547727928 78-79 22.50239616389937 26.371989717091516 27.248785181950424 23.87682893705869 80-81 22.34975546503925 26.655056886815835 27.39023700698162 23.60495064116329 82-83 22.900352239294733 26.21895625662586 27.87227361707993 23.00841788699948 84-85 22.89187263773357 26.436182862412792 27.252364533168226 23.419579966685404 86-87 22.21531624251885 26.75271070641471 27.567052135011604 23.464920916054837 88-89 22.637048517546752 26.015422624747806 27.55722913384448 23.790299723860965 90-91 22.727371647344405 25.99896742518703 27.56234828487292 23.71131264259564 92-93 22.816734725389402 26.09364693715022 27.48982746194074 23.599790875519638 94-95 22.218918627345623 26.094031963161314 27.788019612782982 23.899029796710085 96-97 22.30533551185749 25.965127584812485 27.59802581439198 24.131511088938048 98-99 22.02771184703432 26.169233457428913 27.489098886165987 24.313955809370782 100 22.430980911576388 25.96224600415804 27.111697022836047 24.495076061429526 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1110.0 1 821.5 2 1403.0 3 2029.5 4 1325.0 5 961.0 6 1380.0 7 2409.5 8 3745.0 9 4423.0 10 3928.5 11 3347.0 12 3470.0 13 3832.5 14 4354.5 15 4957.0 16 5406.0 17 5758.5 18 6290.0 19 6921.5 20 7303.0 21 7797.0 22 8656.5 23 9893.5 24 11509.0 25 13548.5 26 16436.0 27 19853.5 28 24082.0 29 30628.5 30 39881.0 31 49309.0 32 60985.0 33 73936.0 34 85016.0 35 96211.0 36 107603.5 37 123350.5 38 132178.5 39 131500.5 40 129778.5 41 128048.5 42 130692.5 43 138709.5 44 149716.5 45 166062.0 46 182984.5 47 195839.0 48 207485.0 49 212746.0 50 211274.0 51 213528.0 52 211690.0 53 202988.5 54 193258.5 55 174216.5 56 155179.0 57 133988.5 58 113222.0 59 100920.5 60 84477.5 61 65838.5 62 51822.0 63 41551.0 64 31044.0 65 20661.5 66 14460.0 67 10557.5 68 7963.5 69 6251.5 70 5074.0 71 3926.0 72 3089.5 73 2529.5 74 2003.5 75 1533.0 76 1094.5 77 714.0 78 472.0 79 321.0 80 232.0 81 177.5 82 131.5 83 108.5 84 92.0 85 67.0 86 57.5 87 49.0 88 37.0 89 25.5 90 17.5 91 14.5 92 14.5 93 12.0 94 8.5 95 10.5 96 8.5 97 5.5 98 3.5 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.057565799698370126 2 0.1308803422947825 3 0.016494735982686328 4 0.016432569892299322 5 0.01933365411035973 6 0.05172218720199131 7 0.05638464398101696 8 0.047971499748641774 9 0.03978629784768562 10-11 0.03870875228097747 12-13 0.03949618942587958 14-15 0.017375422263168953 16-17 0.016515458012815332 18-19 0.016733039329169863 20-21 0.01997603704435882 22-23 0.024338024386513935 24-25 0.023270839834870286 26-27 0.0184115237696191 28-29 0.01748939342887847 30-31 0.015696937822719716 32-33 0.02210004513258162 34-35 0.021892824831291593 36-37 0.02167524351493706 38-39 0.01996567602929432 40-41 0.023623114347063337 42-43 0.030461384289634304 44-45 0.03920608100407354 46-47 0.04540196801264541 48-49 0.03774517787997883 50-51 0.026016508826963176 52-53 0.03839792182904242 54-55 0.035413949490466 56-57 0.03698882378027023 58-59 0.04196211101123093 60-61 0.022120767162710622 62-63 0.024597049763126475 64-65 0.023954666829127384 66-67 0.030078026732247746 68-69 0.03706135088572173 70-71 0.03852225400981644 72-73 0.04329868195455162 74-75 0.025177266606738552 76-77 0.019354376140488735 78-79 0.018287191588845085 80-81 0.02308434156370926 82-83 0.019416542230875744 84-85 0.011801196158467166 86-87 0.013593651764625918 88-89 0.01776914083562001 90-91 0.019685928622552782 92-93 0.018743076251683147 94-95 0.022203655283226636 96-97 0.022980731413064247 98-99 0.02642058841447873 100 0.028845065939572076 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4825782.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.20146138382086 #Duplication Level Percentage of deduplicated Percentage of total 1 80.49222589958762 54.8968743638851 2 12.528779102407286 17.08962088278504 3 3.4638996229697896 7.087290491202173 4 1.3327102795051362 3.6357115465396252 5 0.6623272886624146 2.258584450058022 6 0.3810832412050044 1.5594260375438636 7 0.2440782217537591 1.1652543990899464 8 0.17444508688870647 0.9517927885629909 9 0.1118802581073561 0.6867357392628682 >10 0.5708820061138703 6.6325320627647315 >50 0.02552511515036608 1.1826143692220643 >100 0.011208702087529498 1.3345171733274823 >500 6.014069415193614E-4 0.27278603479109326 >1k 2.47638033746219E-4 0.3476054234124846 >5k 3.5376861963737496E-5 0.1954813035668509 >10k+ 7.075372392747499E-5 0.7031729339857199 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16270 0.3371474301988776 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12874 0.2667754158807837 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8102 0.1678898881051817 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.0722030129002926E-5 6.216609038700878E-5 2 0.0 0.0 0.0 2.0722030129002926E-5 6.216609038700878E-5 3 0.0 0.0 0.0 2.0722030129002926E-5 6.216609038700878E-5 4 0.0 0.0 0.0 2.0722030129002926E-5 6.216609038700878E-5 5 0.0 0.0 0.0 2.0722030129002926E-5 6.216609038700878E-5 6 0.0 0.0 0.0 4.144406025800585E-5 8.28881205160117E-5 7 0.0 0.0 0.0 4.144406025800585E-5 8.28881205160117E-5 8 0.0 0.0 0.0 4.144406025800585E-5 1.0361015064501463E-4 9 0.0 0.0 0.0 4.144406025800585E-5 1.2433218077401756E-4 10-11 0.0 0.0 0.0 5.1805075322507315E-5 1.2433218077401756E-4 12-13 0.0 0.0 0.0 6.216609038700878E-5 1.2433218077401756E-4 14-15 0.0 0.0 0.0 8.28881205160117E-5 1.7613725609652488E-4 16-17 0.0 0.0 0.0 1.3469319583851903E-4 2.2794233141903219E-4 18-19 0.0 0.0 0.0 2.1758131635453074E-4 3.1083045193504386E-4 20-21 0.0 1.0361015064501463E-5 0.0 3.0046943687054244E-4 3.315524820640468E-4 22-23 0.0 2.0722030129002926E-5 0.0 6.216609038700877E-4 3.419134971285483E-4 24-25 0.0 2.0722030129002926E-5 0.0 0.0013262099282561873 3.7299654232205267E-4 26-27 0.0 2.0722030129002926E-5 0.0 0.002486643615480351 3.7299654232205267E-4 28-29 0.0 3.108304519350439E-5 0.0 0.006641410656345438 3.8335755738655414E-4 30-31 0.0 4.144406025800585E-5 0.0 0.021623438439614555 3.937185724510556E-4 32-33 0.0 4.144406025800585E-5 0.0 0.05474760360082573 3.937185724510556E-4 34-35 0.0 4.144406025800585E-5 0.0 0.10213888650585543 3.937185724510556E-4 36-37 0.0 4.144406025800585E-5 0.0 0.17906942335977877 3.937185724510556E-4 38-39 0.0 4.144406025800585E-5 0.0 0.29340322459655244 3.937185724510556E-4 40-41 0.0 4.144406025800585E-5 0.0 0.42076082176940444 3.937185724510556E-4 42-43 0.0 4.144406025800585E-5 0.0 0.5398814119659778 3.937185724510556E-4 44-45 0.0 4.144406025800585E-5 0.0 0.675880095702624 3.937185724510556E-4 46-47 0.0 4.144406025800585E-5 0.0 0.8367141325488802 3.937185724510556E-4 48-49 0.0 4.144406025800585E-5 0.0 1.0109553228886012 3.937185724510556E-4 50-51 0.0 4.144406025800585E-5 0.0 1.1923248915926994 3.937185724510556E-4 52-53 0.0 4.144406025800585E-5 0.0 1.3683481765235148 4.2480161764456E-4 54-55 0.0 4.144406025800585E-5 0.0 1.5637983647002702 4.5588466283806437E-4 56-57 0.0 4.144406025800585E-5 0.0 1.7821153131243808 4.5588466283806437E-4 58-59 0.0 4.144406025800585E-5 0.0 2.043368307975785 4.5588466283806437E-4 60-61 0.0 4.144406025800585E-5 0.0 2.2934832116328505 4.5588466283806437E-4 62-63 0.0 4.144406025800585E-5 0.0 2.5478150484211675 4.5588466283806437E-4 64-65 0.0 4.144406025800585E-5 0.0 2.8083531332331217 4.7660669296706727E-4 66-67 0.0 4.144406025800585E-5 0.0 3.1006684512479015 4.973287230960702E-4 68-69 0.0 4.144406025800585E-5 0.0 3.4205129862890615 4.973287230960702E-4 70-71 0.0 4.144406025800585E-5 0.0 3.760323197359516 4.973287230960702E-4 72-73 0.0 4.144406025800585E-5 0.0 4.083690477522607 5.180507532250732E-4 74-75 0.0 4.144406025800585E-5 0.0 4.403897648091025 5.180507532250732E-4 76-77 0.0 4.144406025800585E-5 0.0 4.759218298713037 5.180507532250732E-4 78-79 0.0 4.144406025800585E-5 0.0 5.150605642774581 5.180507532250732E-4 80-81 0.0 4.144406025800585E-5 0.0 5.559057578647357 5.180507532250732E-4 82-83 0.0 4.144406025800585E-5 0.0 5.971291285018677 5.180507532250732E-4 84-85 0.0 4.144406025800585E-5 0.0 6.38148387142229 5.180507532250732E-4 86-87 0.0 5.1805075322507315E-5 0.0 6.808212637868847 5.180507532250732E-4 88 0.0 6.216609038700878E-5 0.0 7.154964729032518 5.180507532250732E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8285 0.0 48.40075 1 GTATCAA 14455 0.0 40.86304 1 TACCTGG 3760 0.0 39.80437 2 GTACTAG 3005 0.0 34.45746 1 CAACGCA 17225 0.0 33.052082 5 ATCAACG 17275 0.0 32.928864 3 TATCAAC 18435 0.0 31.75914 2 GCATCGT 2960 0.0 31.12639 8 AACGCAG 18975 0.0 30.622883 6 GTACCTG 4995 0.0 30.529139 1 TCAACGC 18790 0.0 30.449013 4 ACCTGGG 4765 0.0 29.993013 3 GTGGTAT 3260 0.0 29.307817 1 GTACATG 19275 0.0 27.592375 1 ACGCAGA 20860 0.0 27.202095 7 TAGGCAT 3890 0.0 26.950645 5 TACATGG 19635 0.0 26.726091 2 CGCAGAG 21315 0.0 26.596615 8 CTGTGCG 4045 0.0 26.496021 9 TAGTACT 4065 0.0 26.252745 4 >>END_MODULE