FastQCFastQC Report
Mon 6 Jun 2016
ERR1211255_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211255_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4825782
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149910.31064395366388287No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124640.2582793835278925No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80280.1663564578756355No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79100.048.0949551
GTATCAA140200.041.9265861
TACCTGG39800.038.969042
ATCAACG172350.033.4362533
CAACGCA173900.033.072515
ACCTGGG47900.031.990463
TATCAAC184100.031.9368722
GTGGTAT31250.031.6007041
GTACTAG28800.031.5131871
GTACCTG50750.030.8557191
TCAACGC188900.030.7058814
AACGCAG192350.030.4376546
GTACATG193450.027.8575781
ACGCAGA211000.027.2796617
TACATGG194100.027.0952642
CGCAGAG215700.026.685258
TAGGCAT38350.026.2200645
ACATGGG194700.026.1215953
TGGTATC36900.025.0916212
TGGGGGG109250.024.9024726
Produced by FastQC (version 0.11.5)