##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211255_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4825782 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15714261439908 33.0 31.0 34.0 30.0 34.0 2 32.29143318119219 34.0 31.0 34.0 30.0 34.0 3 32.371350177028305 34.0 31.0 34.0 30.0 34.0 4 35.984446458625776 37.0 35.0 37.0 35.0 37.0 5 35.8723775752821 37.0 35.0 37.0 35.0 37.0 6 35.90196055271456 37.0 35.0 37.0 35.0 37.0 7 35.84020765960833 37.0 35.0 37.0 35.0 37.0 8 35.824096902843934 37.0 35.0 37.0 35.0 37.0 9 37.43335525724121 39.0 37.0 39.0 34.0 39.0 10-11 37.408741008193076 39.0 37.0 39.0 34.0 39.0 12-13 37.30921983214327 39.0 37.0 39.0 34.0 39.0 14-15 38.536344679473714 40.0 38.0 41.0 34.0 41.0 16-17 38.48457566048363 40.0 38.0 41.0 34.0 41.0 18-19 38.50284710332957 40.0 38.0 41.0 34.0 41.0 20-21 38.460817334889974 40.0 38.0 41.0 34.0 41.0 22-23 38.33282895911999 40.0 38.0 41.0 34.0 41.0 24-25 38.266588813999476 40.0 38.0 41.0 34.0 41.0 26-27 38.12963546633478 40.0 37.5 41.0 33.0 41.0 28-29 38.09771255726015 40.0 37.5 41.0 33.0 41.0 30-31 38.109900322890674 40.0 37.5 41.0 33.5 41.0 32-33 37.97332981473262 40.0 37.0 41.0 33.0 41.0 34-35 37.82760037647785 40.0 37.0 41.0 33.0 41.0 36-37 37.68140800806999 40.0 37.0 41.0 33.0 41.0 38-39 37.591209466154915 40.0 37.0 41.0 33.0 41.0 40-41 37.48719482148178 40.0 36.0 41.0 32.0 41.0 42-43 37.340809012922676 40.0 36.0 41.0 32.0 41.0 44-45 37.200091715705355 39.5 36.0 41.0 31.0 41.0 46-47 36.95895421716108 39.0 36.0 41.0 31.0 41.0 48-49 36.7280328866907 39.0 35.0 41.0 30.5 41.0 50-51 36.53049858033371 39.0 35.0 41.0 30.0 41.0 52-53 36.31981510975838 39.0 35.0 40.0 30.0 41.0 54-55 36.0770552834753 38.0 35.0 40.0 29.0 41.0 56-57 35.81708197759451 38.0 35.0 40.0 28.5 41.0 58-59 35.53919199002358 38.0 34.0 40.0 28.0 41.0 60-61 35.26362131567485 37.0 34.0 40.0 28.0 41.0 62-63 34.948487726963215 37.0 34.0 40.0 27.5 41.0 64-65 34.56769887243145 36.0 33.5 39.0 26.5 41.0 66-67 34.25628696033099 36.0 33.0 39.0 26.0 40.0 68-69 33.922720814989155 35.0 33.0 38.5 26.0 40.0 70-71 33.59765743666001 35.0 33.0 38.0 26.0 40.0 72-73 33.25410399806705 35.0 32.0 37.0 26.0 39.5 74-75 32.91510546891675 35.0 32.0 36.5 25.0 39.0 76-77 31.933844089931952 34.0 30.5 35.5 24.5 38.0 78-79 32.4283826952813 35.0 32.0 36.0 25.0 38.0 80-81 32.36294190247301 35.0 32.0 35.5 25.0 37.0 82-83 32.120636717530964 34.5 32.0 35.0 25.0 37.0 84-85 31.87610090965568 34.0 31.5 35.0 24.5 36.5 86-87 31.646317736690136 34.0 31.0 35.0 24.0 36.0 88-89 31.439368997604948 34.0 31.0 35.0 24.0 36.0 90-91 31.233831843212144 34.0 31.0 35.0 23.0 35.0 92-93 31.05351754389237 34.0 31.0 35.0 23.0 35.0 94-95 30.863506059743273 34.0 30.0 35.0 20.5 35.0 96-97 30.6971444213601 34.0 30.0 35.0 20.0 35.0 98-99 30.52358726523494 34.0 30.0 35.0 20.0 35.0 100 28.740461338701166 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.27167006054216003 1101 2 -0.28602213013003563 1101 3 -0.3551592174442675 1101 4 -0.11217516890091872 1101 5 -0.18498272501651059 1101 6 -0.31436688418205705 1101 7 -0.2647655865363774 1101 8 -0.36408588359180527 1101 9 -0.4995848871223316 1101 10-11 -0.4442964170951811 1101 12-13 -0.4595179462724488 1101 14-15 -0.7372208478041529 1101 16-17 -0.6075902356818048 1101 18-19 -0.6524612680965873 1101 20-21 -0.6835033088918081 1101 22-23 -0.7438932750552425 1101 24-25 -0.7598399164366398 1101 26-27 -0.8415388278302061 1101 28-29 -0.8054524574870072 1101 30-31 -0.59977742948508 1101 32-33 -0.6617320060874974 1101 34-35 -0.6673780324344847 1101 36-37 -0.5785973730752048 1101 38-39 -0.6039056714549176 1101 40-41 -0.7089457886855186 1101 42-43 -0.7645020068528936 1101 44-45 -0.6269725676642395 1101 46-47 -0.6876940625454111 1101 48-49 -0.6169000829139506 1101 50-51 -0.7103272390021402 1101 52-53 -0.6927669360401438 1101 54-55 -0.5502441123360029 1101 56-57 -0.5592088503092825 1101 58-59 -0.7077072357154535 1101 60-61 -0.7578309698926233 1101 62-63 -0.9221324378082372 1101 64-65 -0.7751743090526944 1101 66-67 -0.7668342501924812 1101 68-69 -0.8736463084051991 1101 70-71 -0.8414346123603238 1101 72-73 -0.8324238854760395 1101 74-75 -0.8959339392177981 1101 76-77 -0.876644232231012 1101 78-79 -0.6824530388077861 1101 80-81 -0.6547285772772931 1101 82-83 -0.5498294689035852 1101 84-85 -0.62170752415431 1101 86-87 -0.789363885736595 1101 88-89 -0.7380236954961177 1101 90-91 -0.7453017137631477 1101 92-93 -0.79943003483789 1101 94-95 -0.8490943490783032 1101 96-97 -1.0126163358925133 1101 98-99 -1.2572995507603437 1101 100 -1.200791950910947 1102 1 -0.4481238958345841 1102 2 -0.3955222822135802 1102 3 -0.45527693405798075 1102 4 -0.2730536631465412 1102 5 -0.28070669755280875 1102 6 -0.31775096250530765 1102 7 -0.3752726063002143 1102 8 -0.42917237370110684 1102 9 -0.3917056976376472 1102 10-11 -0.5351697639821609 1102 12-13 -0.5486486692113388 1102 14-15 -0.6701922475389566 1102 16-17 -0.746961252815673 1102 18-19 -0.7018616737843857 1102 20-21 -0.6906720290155093 1102 22-23 -0.7498699608453734 1102 24-25 -0.8239620860477643 1102 26-27 -0.8061944913731196 1102 28-29 -0.8437561685449566 1102 30-31 -0.713821077462363 1102 32-33 -0.6684237369123522 1102 34-35 -0.6820542865092563 1102 36-37 -0.7805937000271754 1102 38-39 -0.835900861620793 1102 40-41 -0.7624488016075759 1102 42-43 -0.7337835723082975 1102 44-45 -0.7081621923624368 1102 46-47 -0.7793014923422845 1102 48-49 -0.8953087349094844 1102 50-51 -0.8290709652816091 1102 52-53 -0.8565365263977469 1102 54-55 -0.8232120468483473 1102 56-57 -0.7798588384805569 1102 58-59 -0.7856236077614014 1102 60-61 -0.9233872675778372 1102 62-63 -0.9991665661895723 1102 64-65 -0.9139478276865489 1102 66-67 -1.0586921460058178 1102 68-69 -1.0312614255534172 1102 70-71 -0.9009461323047745 1102 72-73 -1.0248887109442606 1102 74-75 -1.1488536744388114 1102 76-77 -0.9777856324003444 1102 78-79 -0.6692464867502252 1102 80-81 -0.7468011457072095 1102 82-83 -0.6923904822759646 1102 84-85 -0.7534372484332081 1102 86-87 -0.7495869064888687 1102 88-89 -0.9789445218562101 1102 90-91 -0.9804457245902682 1102 92-93 -0.9734985670188081 1102 94-95 -0.9379936822808403 1102 96-97 -1.0551418192131372 1102 98-99 -1.2180339606608257 1102 100 -1.232884322022887 1103 1 -0.36644104951601264 1103 2 -0.3697181574881796 1103 3 -0.314189276510767 1103 4 -0.2231853046779051 1103 5 -0.24655782403215198 1103 6 -0.3555095456055355 1103 7 -0.395497242151599 1103 8 -0.3382717991453319 1103 9 -0.5006035090625929 1103 10-11 -0.5115511750397133 1103 12-13 -0.583620116007765 1103 14-15 -0.655058128813323 1103 16-17 -0.6938768531994199 1103 18-19 -0.6397687554728222 1103 20-21 -0.6232243737166172 1103 22-23 -0.7576447526950503 1103 24-25 -0.8158534265345452 1103 26-27 -0.7964184097217313 1103 28-29 -0.8500973919957531 1103 30-31 -0.7639846074141659 1103 32-33 -0.7284295897761695 1103 34-35 -0.8568686992023657 1103 36-37 -0.8383647819404487 1103 38-39 -0.8630956802288594 1103 40-41 -0.8478072527895009 1103 42-43 -0.7755807964455101 1103 44-45 -0.828964375359277 1103 46-47 -0.9606934572906809 1103 48-49 -0.9837330466710412 1103 50-51 -0.9444548313909138 1103 52-53 -0.9310249969586266 1103 54-55 -1.0011004904975493 1103 56-57 -0.966153880940972 1103 58-59 -1.0066023241170114 1103 60-61 -1.000393278632913 1103 62-63 -0.9725914719667657 1103 64-65 -0.9391251754216015 1103 66-67 -0.9688590164972126 1103 68-69 -0.9731014922705299 1103 70-71 -1.0822702591762905 1103 72-73 -1.0162473003040233 1103 74-75 -0.9769912959405609 1103 76-77 -0.9176892666689582 1103 78-79 -0.8498224785966677 1103 80-81 -0.7788012634457928 1103 82-83 -0.8362758949550262 1103 84-85 -0.8897141510610567 1103 86-87 -0.9701955872929666 1103 88-89 -0.9927404553400834 1103 90-91 -1.002507364465199 1103 92-93 -1.1508816715532149 1103 94-95 -1.1120745708175335 1103 96-97 -1.182979335069522 1103 98-99 -1.115733661694243 1103 100 -1.2533907427145614 1104 1 -0.4368963307436502 1104 2 -0.356661506295584 1104 3 -0.42514704707717854 1104 4 -0.2995778720374318 1104 5 -0.33487275337320455 1104 6 -0.3438903382456928 1104 7 -0.389143450649847 1104 8 -0.4724514638856121 1104 9 -0.6171625263934288 1104 10-11 -0.5528387029969579 1104 12-13 -0.606921928973513 1104 14-15 -0.7453970074216087 1104 16-17 -0.741112715729308 1104 18-19 -0.8189972386865847 1104 20-21 -0.8460182398307765 1104 22-23 -0.7070098269478677 1104 24-25 -0.7853744005400642 1104 26-27 -0.7526740368697915 1104 28-29 -0.8726154952828296 1104 30-31 -0.7813165361362948 1104 32-33 -0.7293531247685578 1104 34-35 -0.7220068974768452 1104 36-37 -0.7808318247544079 1104 38-39 -0.8584504623379701 1104 40-41 -0.8644188440213654 1104 42-43 -0.7899252945409074 1104 44-45 -0.894575201955675 1104 46-47 -0.9693150194276328 1104 48-49 -0.8678355026143478 1104 50-51 -0.9261793032910788 1104 52-53 -0.983654583182016 1104 54-55 -0.8998318637124214 1104 56-57 -0.8726721899791912 1104 58-59 -0.9765661477697236 1104 60-61 -0.9977560106162926 1104 62-63 -0.9661613836855594 1104 64-65 -1.0665398590495059 1104 66-67 -1.139050552682832 1104 68-69 -1.0469917702055227 1104 70-71 -1.1995972994845516 1104 72-73 -1.0957240242620614 1104 74-75 -1.0155001410512199 1104 76-77 -0.9482757543842375 1104 78-79 -0.861091288827609 1104 80-81 -0.8079756092999268 1104 82-83 -0.8056970942445076 1104 84-85 -0.7627685571271066 1104 86-87 -0.8632319062276785 1104 88-89 -1.013448594726377 1104 90-91 -0.9033707219362483 1104 92-93 -0.9680916296587796 1104 94-95 -1.0225999981785137 1104 96-97 -0.9617186746270896 1104 98-99 -0.9769957073609916 1104 100 -1.1943984793330742 1105 1 -0.3768045234184747 1105 2 -0.35930180977263504 1105 3 -0.406030005508466 1105 4 -0.2568118622028308 1105 5 -0.30647036021186125 1105 6 -0.39321843653858224 1105 7 -0.36053840911382906 1105 8 -0.41727808126688615 1105 9 -0.5258849885379746 1105 10-11 -0.5556770828724993 1105 12-13 -0.5870553131860348 1105 14-15 -0.7868629924296116 1105 16-17 -0.7749282520706444 1105 18-19 -0.7218708406580063 1105 20-21 -0.7771755423750406 1105 22-23 -0.741125274710356 1105 24-25 -0.691182112927514 1105 26-27 -0.7459542535457047 1105 28-29 -0.7483786832630841 1105 30-31 -0.7322828322430155 1105 32-33 -0.692022045240698 1105 34-35 -0.7885516277506923 1105 36-37 -0.7367229054674098 1105 38-39 -0.7351428597801188 1105 40-41 -0.7565900371998779 1105 42-43 -0.8049431635606794 1105 44-45 -0.7972988993000456 1105 46-47 -0.8456241882065996 1105 48-49 -0.9183269006582506 1105 50-51 -0.9134292110853011 1105 52-53 -0.8882358310301157 1105 54-55 -0.8042842788907762 1105 56-57 -0.9490798733512023 1105 58-59 -0.8576394434442776 1105 60-61 -0.7805427246067467 1105 62-63 -0.7841871277427543 1105 64-65 -0.8065177296630921 1105 66-67 -0.8385767018324444 1105 68-69 -0.8424446461573325 1105 70-71 -1.0037327158000195 1105 72-73 -0.9125800831266417 1105 74-75 -0.8813097026625556 1105 76-77 -0.892355300639867 1105 78-79 -0.8233440062161357 1105 80-81 -0.6897055837888111 1105 82-83 -0.7129847221193586 1105 84-85 -0.7381835342059269 1105 86-87 -0.8163962501261075 1105 88-89 -0.8892006464964766 1105 90-91 -0.8573976112862489 1105 92-93 -0.8245903745363776 1105 94-95 -0.8536845577720555 1105 96-97 -0.9939215293792394 1105 98-99 -1.0680723081401169 1105 100 -1.1868154864714882 1106 1 -0.44594793805854493 1106 2 -0.468541010730398 1106 3 -0.49391944096159435 1106 4 -0.27579049392154076 1106 5 -0.34471391010318087 1106 6 -0.4307580323733404 1106 7 -0.47182223868460227 1106 8 -0.43858064668996377 1106 9 -0.5631478593501384 1106 10-11 -0.599321197292987 1106 12-13 -0.654250598843916 1106 14-15 -0.7055378589932673 1106 16-17 -0.7762245342674348 1106 18-19 -0.8370951940788629 1106 20-21 -0.8220845987452421 1106 22-23 -0.7587803058571012 1106 24-25 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2311 30-31 0.9289781294590469 2311 32-33 0.9681714759909212 2311 34-35 1.0028344999026046 2311 36-37 1.0192867997902297 2311 38-39 1.078002241404512 2311 40-41 1.041401354827812 2311 42-43 0.9813807116001954 2311 44-45 1.0209526134209241 2311 46-47 0.7981941336199654 2311 48-49 1.0504967535046532 2311 50-51 1.110393924036508 2311 52-53 1.0043194975350644 2311 54-55 1.123631272310604 2311 56-57 1.2205467130695524 2311 58-59 1.2048431301561564 2311 60-61 1.187093886606327 2311 62-63 1.1611978466660844 2311 64-65 1.181474497696378 2311 66-67 1.1613392581138058 2311 68-69 1.22770836148311 2311 70-71 1.2252430174063633 2311 72-73 1.1994871359403376 2311 74-75 1.251986338108118 2311 76-77 1.1663446798149941 2311 78-79 0.883492353099804 2311 80-81 0.8538429380388948 2311 82-83 0.9664547215983319 2311 84-85 1.0831166150994953 2311 86-87 1.1102591260448769 2311 88-89 1.1293484430324412 2311 90-91 1.2134740452577688 2311 92-93 1.2784171645356537 2311 94-95 1.3041317957533352 2311 96-97 1.3599437453922043 2311 98-99 1.3352600776728636 2311 100 1.4520622331214206 2312 1 0.18717621110418037 2312 2 0.28928857906787186 2312 3 0.42662390162436736 2312 4 0.13164089280851243 2312 5 0.2981944779870176 2312 6 0.4008712529656506 2312 7 0.28589374394176303 2312 8 0.4212018464210061 2312 9 0.6123492822576679 2312 10-11 0.6416539343751495 2312 12-13 0.6824199253026322 2312 14-15 0.8838566652284356 2312 16-17 0.8563459586848126 2312 18-19 0.8280600205280635 2312 20-21 0.8297792718979693 2312 22-23 0.9256739667993941 2312 24-25 0.8867325316846788 2312 26-27 0.9774257134655713 2312 28-29 0.9490910686300111 2312 30-31 0.9904604099073637 2312 32-33 0.8803054599260847 2312 34-35 0.9660763211801822 2312 36-37 1.0531031079343336 2312 38-39 0.7604657972458639 2312 40-41 0.9285824839615273 2312 42-43 0.9934198192858616 2312 44-45 0.736254517810579 2312 46-47 0.7718981644246057 2312 48-49 1.0022884079607621 2312 50-51 0.8932890270363671 2312 52-53 1.0591991577203004 2312 54-55 0.9076408799079445 2312 56-57 0.9713004090022181 2312 58-59 1.099824756678565 2312 60-61 1.0690679659488254 2312 62-63 1.0994009730292262 2312 64-65 1.132974770181427 2312 66-67 1.1207267755349548 2312 68-69 1.170182829462263 2312 70-71 1.0888479016069041 2312 72-73 1.0695555163781094 2312 74-75 1.1666014388104173 2312 76-77 1.1946317989456539 2312 78-79 1.0430984635148945 2312 80-81 0.9732653206827919 2312 82-83 0.9590986760409237 2312 84-85 0.9891497740907873 2312 86-87 0.9786490234277316 2312 88-89 0.9648114612905303 2312 90-91 1.0965691722775688 2312 92-93 1.0980859840825445 2312 94-95 1.1546966084136514 2312 96-97 0.8008734574954168 2312 98-99 0.5796834473506998 2312 100 0.7413275576511786 2313 1 -0.023075335040132927 2313 2 0.178920973645603 2313 3 0.2662622726266122 2313 4 0.18664435059697837 2313 5 0.30297194996906995 2313 6 0.2375582983981417 2313 7 0.2577290317583092 2313 8 0.3937992185498729 2313 9 0.5602170635455437 2313 10-11 0.35219610971430626 2313 12-13 0.4121575915317095 2313 14-15 0.510044314255758 2313 16-17 0.7416140151527628 2313 18-19 0.7406663260287019 2313 20-21 0.6816118186159983 2313 22-23 0.6646726709855031 2313 24-25 0.6019199491105098 2313 26-27 0.7640011158009941 2313 28-29 0.5656822122903975 2313 30-31 0.7947306584123837 2313 32-33 0.9116202709129411 2313 34-35 0.8648020651037314 2313 36-37 0.9800720722536411 2313 38-39 0.8972095014674437 2313 40-41 0.943934259073842 2313 42-43 0.9975441233841309 2313 44-45 0.8315979842498251 2313 46-47 0.885277686380384 2313 48-49 1.0541238647285454 2313 50-51 0.9995867865746249 2313 52-53 1.0180253055755912 2313 54-55 0.9611016956670397 2313 56-57 0.6168821417781274 2313 58-59 0.7984495567479186 2313 60-61 0.6033589792221434 2313 62-63 1.0080716917598096 2313 64-65 1.0449935943553825 2313 66-67 0.9818501972388063 2313 68-69 1.1883268243761762 2313 70-71 1.0933670766102068 2313 72-73 1.1284812282189733 2313 74-75 1.1386569344213555 2313 76-77 1.0635427452902313 2313 78-79 1.0250971752767484 2313 80-81 0.9133401510491339 2313 82-83 0.9260507320091236 2313 84-85 0.9535715297670997 2313 86-87 0.9808634269956009 2313 88-89 1.0544538907299454 2313 90-91 0.8918230464616883 2313 92-93 1.1046679725099295 2313 94-95 1.2496670293210812 2313 96-97 1.2832523245697551 2313 98-99 1.2325364865910373 2313 100 1.2826633414388091 2314 1 -0.37393438045624094 2314 2 0.052352161681625375 2314 3 0.2882462506169574 2314 4 0.21755691263457066 2314 5 0.33615908619384527 2314 6 0.36655596882337704 2314 7 0.40401878940244984 2314 8 0.40279988044118653 2314 9 0.3801563626945068 2314 10-11 0.5577763716913537 2314 12-13 0.4515453656218469 2314 14-15 0.744934525095232 2314 16-17 0.7866464148503169 2314 18-19 0.764322580666061 2314 20-21 0.8065514593102989 2314 22-23 0.8851794064951406 2314 24-25 0.7568303111250145 2314 26-27 0.689693863969012 2314 28-29 0.7505577602351181 2314 30-31 0.7658069502193001 2314 32-33 0.8291637472791891 2314 34-35 0.8744364113741767 2314 36-37 0.8077252906707102 2314 38-39 0.5765957680471132 2314 40-41 0.5827601910804106 2314 42-43 0.8442586338789937 2314 44-45 0.9031144573348797 2314 46-47 0.8523121424164302 2314 48-49 0.877326335161321 2314 50-51 0.8856830646726195 2314 52-53 0.9462703985112597 2314 54-55 0.9282046963195114 2314 56-57 0.8814300888550832 2314 58-59 1.0057310089515923 2314 60-61 1.0177303826908215 2314 62-63 0.9396082218004906 2314 64-65 0.9972969643479175 2314 66-67 0.7006562725059737 2314 68-69 0.7535553888728899 2314 70-71 0.8616398111635206 2314 72-73 1.0288622279339634 2314 74-75 1.1650326554942296 2314 76-77 0.9111106480369635 2314 78-79 0.8146702082700301 2314 80-81 0.9072696537355611 2314 82-83 0.8923556354224544 2314 84-85 0.923835238101514 2314 86-87 0.984727246980011 2314 88-89 0.9498065775140248 2314 90-91 1.0264999681253215 2314 92-93 1.0071999423314537 2314 94-95 0.9905757401405353 2314 96-97 1.016596100553695 2314 98-99 1.081160529969651 2314 100 1.1492086221066344 2315 1 0.4022758834735498 2315 2 0.427090087568601 2315 3 0.4385959180358938 2315 4 0.312600987198131 2315 5 0.36722355145560215 2315 6 0.4439665124871013 2315 7 0.3506399551918591 2315 8 0.24077502670451167 2315 9 0.4618691684035241 2315 10-11 0.5324776104374465 2315 12-13 0.3587655272864154 2315 14-15 0.6366305267703396 2315 16-17 0.7485761466974665 2315 18-19 0.7509251720280687 2315 20-21 0.46319459621229697 2315 22-23 0.8427480886459904 2315 24-25 0.9094167030924822 2315 26-27 0.8801514136385578 2315 28-29 0.844527570614801 2315 30-31 0.8581766277900584 2315 32-33 0.7651631255828661 2315 34-35 0.8962073401014266 2315 36-37 0.8984751170133407 2315 38-39 0.8988589073370932 2315 40-41 0.9188002266764954 2315 42-43 0.9754951243356373 2315 44-45 1.0062448188774553 2315 46-47 0.9941937119143773 2315 48-49 0.859440787865644 2315 50-51 1.0164938078438155 2315 52-53 1.0885342641919138 2315 54-55 0.942133752358636 2315 56-57 0.8589627661429304 2315 58-59 0.868881827067483 2315 60-61 0.9962876384515127 2315 62-63 1.1138727144710003 2315 64-65 1.1567338233522477 2315 66-67 1.1225949033742282 2315 68-69 1.1419092728868208 2315 70-71 1.0515236739386253 2315 72-73 1.0446832523271539 2315 74-75 0.44519579343140947 2315 76-77 0.5171713195983862 2315 78-79 0.8375110781272852 2315 80-81 0.9081824797952862 2315 82-83 0.7595156301759687 2315 84-85 0.8148289493815035 2315 86-87 0.8671046266447142 2315 88-89 0.8627460218222041 2315 90-91 0.9500052943041482 2315 92-93 0.902484166949101 2315 94-95 1.0014701299929456 2315 96-97 1.091483157095123 2315 98-99 1.1712417092752219 2315 100 1.0021155376400444 2316 1 0.3454790012806157 2316 2 0.1520598132215909 2316 3 0.307264653145495 2316 4 0.09886510859485753 2316 5 0.3473047854583058 2316 6 0.38994754510636653 2316 7 0.44318435368360554 2316 8 0.48065118042072186 2316 9 0.6346070166970179 2316 10-11 0.6782770277524506 2316 12-13 0.42158346728714946 2316 14-15 0.562764857374205 2316 16-17 0.8508500181442926 2316 18-19 0.7911266293297459 2316 20-21 0.8074841609871797 2316 22-23 0.8491364573170586 2316 24-25 0.550783987662733 2316 26-27 0.7762559487097889 2316 28-29 0.9239474867778767 2316 30-31 0.5657637647518143 2316 32-33 0.7850779784048214 2316 34-35 0.8917326460787649 2316 36-37 0.8834836543308242 2316 38-39 0.8101707013222139 2316 40-41 0.8125320584409081 2316 42-43 0.7287662091619325 2316 44-45 0.6107977412324104 2316 46-47 0.7133027320121812 2316 48-49 0.7585601387463541 2316 50-51 0.7884117447755585 2316 52-53 0.8764756868589103 2316 54-55 0.7688224260199519 2316 56-57 0.8737775643475132 2316 58-59 0.7700164632507764 2316 60-61 0.8075161917224492 2316 62-63 0.8637016999147846 2316 64-65 0.9139286798745658 2316 66-67 0.900972085815873 2316 68-69 0.9059193419706375 2316 70-71 0.8954557327727244 2316 72-73 0.9287603132141413 2316 74-75 0.8694641109832091 2316 76-77 0.7882942663794701 2316 78-79 0.5370404594349196 2316 80-81 0.5678958295885792 2316 82-83 0.44372378683383573 2316 84-85 0.5534074677724199 2316 86-87 0.6472150819545384 2316 88-89 0.6236884692135192 2316 90-91 0.5419958685639443 2316 92-93 0.3333143959032725 2316 94-95 0.3703888686419887 2316 96-97 0.64692849887318 2316 98-99 0.8122172805966237 2316 100 0.2873348412197032 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 0.0 12 15.0 13 105.0 14 453.0 15 1360.0 16 3247.0 17 6517.0 18 10717.0 19 15829.0 20 21519.0 21 29860.0 22 36879.0 23 38709.0 24 39162.0 25 41728.0 26 48738.0 27 59528.0 28 73896.0 29 91115.0 30 112670.0 31 140364.0 32 178069.0 33 234950.0 34 335774.0 35 469114.0 36 728804.0 37 1091250.0 38 883944.0 39 131459.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.211427031397385 19.7570926580494 13.388346998642106 23.643133311911104 2 15.889424760588025 19.937307570047714 40.29396686381606 23.879300805548198 3 19.20022495835908 25.414409519534864 29.299728002632524 26.085637519473526 4 12.817457602875603 16.111052377369127 37.66829808272337 33.4031919370319 5 13.431791987288278 37.4740093937107 34.074228798565706 15.01996982043532 6 31.31003431153749 36.33444279082644 17.84639256394093 14.509130333695138 7 28.17524416427136 31.878919470913004 21.816684546454674 18.12915181836097 8 26.838945480753175 35.21112225956332 19.508734542919676 18.44119771676383 9 26.743060667555323 15.783002241542818 20.62613154805722 36.84780554284464 10-11 26.031892830044956 25.874611757682292 27.11986755234491 20.97362785992784 12-13 25.914134322078198 23.394480251540607 27.822913242134977 22.86847218424622 14-15 23.253323690662995 24.83715301539805 25.676510859142244 26.23301243479671 16-17 22.369632341231664 27.58886647102931 26.644591412491963 23.396909775247057 18-19 22.061450351466352 26.97105878384063 28.862824719392627 22.10466614530039 20-21 23.112616773820285 26.32810599401299 28.537354153171446 22.02192307899528 22-23 23.099914169351205 26.05412967266238 28.340308368674755 22.50564778931166 24-25 22.595050392104365 25.93359465335587 28.90774005150696 22.56361490303281 26-27 22.628821811302068 26.19460581815695 28.30186157399205 22.874710796548932 28-29 22.179141041238367 26.78485913034036 28.596610325433875 22.439389502987396 30-31 23.08918444764677 26.25267787093253 28.539783764383003 22.1183539170377 32-33 23.10125031178262 26.36909487341807 28.07754447002032 22.45211034477899 34-35 23.081006339835668 26.41062742754644 28.440206003723024 22.068160228894868 36-37 22.920084256678578 26.211707963798315 28.217695975338213 22.65051180418489 38-39 22.8353895374432 26.347054809909242 28.491425873434217 22.32612977921334 40-41 22.766407275187575 25.818233247180338 28.80688726598687 22.60847221164521 42-43 22.705026203538722 26.066120509650304 28.253901778976847 22.974951507834124 44-45 22.509872823955615 26.58280970658354 28.329935057123055 22.57738241233779 46-47 22.59208580816771 26.315974915138174 27.820533971467192 23.271405305226924 48-49 22.98505767753877 26.365564895053943 27.825221287535573 22.82415613987171 50-51 22.81326557025211 26.370858421016308 27.745431680850064 23.070444327881518 52-53 22.58126287554415 26.77096662650666 27.44714479352137 23.200625704427814 54-55 22.50466734234372 26.63113991126117 27.59235804486928 23.27183470152583 56-57 23.07208206630401 26.36915061918271 27.244132603872572 23.314634710640707 58-59 22.42487512647961 26.63848928038347 27.800780559883076 23.13585503325384 60-61 22.783218258463172 26.964727780478675 27.331898057715236 22.920155903342913 62-63 22.876743199327354 26.684487850770378 27.08726733382134 23.351501616080924 64-65 22.409373495189687 26.582513718065893 27.258524002236268 23.74958878450816 66-67 22.52517893948066 26.410431668138628 27.276782710591146 23.787606681789562 68-69 22.232220562935378 26.506998464137375 27.457989258810215 23.80279171411703 70-71 22.3055485640857 26.255271764077076 27.525636810026583 23.913542861810637 72-73 22.54397897189892 26.506608442316544 27.32755477942438 23.621857806360154 74-75 22.761224002234616 26.707220062633542 27.017889893280444 23.513666041851394 76-77 22.606529979815672 26.48950751794954 26.940071461329783 23.963891040905004 78-79 22.441833004269487 26.514919063558185 27.0369756675136 24.00627226465873 80-81 22.275945915646595 26.87162187784996 27.085237625631486 23.767194580871955 82-83 22.798697256390117 26.396209099843638 27.55008164432098 23.255011999445273 84-85 22.913065900945146 26.63473165624425 26.881386591753113 23.57081585105749 86-87 22.154897450381817 26.998953256913587 27.17858005158763 23.667569241116965 88-89 22.460754154756312 26.360672972544236 27.146408957207747 24.032163915491704 90-91 22.6138700689129 26.254612852131654 27.138196903480477 23.99332017547497 92-93 22.6451117590155 26.436544405664385 27.034697964532434 23.883645870787678 94-95 22.06826502095634 26.403010641844226 27.304340040792507 24.22438429640692 96-97 22.140387949162452 26.33906225051711 27.138640500422074 24.381909299898368 98-99 21.81157423494373 26.572920370435043 27.0448402661314 24.570665128489825 100 22.032402695526184 26.41366916167975 26.77884206795197 24.7750860748421 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1048.0 1 789.5 2 1205.5 3 1751.0 4 1200.5 5 880.0 6 1276.5 7 2276.5 8 3465.0 9 3879.5 10 3390.5 11 2930.5 12 3075.0 13 3448.0 14 3941.5 15 4554.5 16 5154.5 17 5751.0 18 6342.0 19 6888.5 20 7339.5 21 8004.5 22 8917.5 23 10173.5 24 11837.0 25 13708.0 26 16508.5 27 20042.5 28 24302.0 29 31082.5 30 40390.5 31 49388.0 32 60913.0 33 73938.5 34 85141.5 35 96545.5 36 107593.0 37 122840.5 38 131754.0 39 131228.0 40 129299.5 41 127444.0 42 130263.0 43 137974.0 44 149059.5 45 166960.0 46 184866.5 47 197046.0 48 208562.5 49 214243.0 50 212786.5 51 214102.0 52 213063.0 53 205485.5 54 195857.0 55 176568.5 56 157014.5 57 136204.5 58 114730.0 59 102001.0 60 84232.0 61 64077.0 62 50242.0 63 39898.0 64 29082.0 65 18828.0 66 12834.5 67 9300.0 68 7061.0 69 5313.0 70 4169.5 71 3216.5 72 2513.0 73 2151.0 74 1695.5 75 1182.0 76 798.0 77 483.5 78 289.5 79 177.5 80 113.0 81 76.5 82 46.5 83 31.0 84 23.0 85 16.0 86 9.5 87 6.5 88 4.5 89 2.0 90 1.5 91 2.0 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04417936823503424 2 0.0 3 0.0 4 0.03630499678601313 5 0.0 6 0.0 7 7.252710545151024E-4 8 0.0 9 0.011065564088887562 10-11 5.491337984185775E-4 12-13 1.0361015064501463E-5 14-15 1.968592862255278E-4 16-17 3.419134971285483E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.387727833540761E-4 26-27 5.59494813483079E-4 28-29 1.8649827116102633E-4 30-31 0.0024866436154803513 32-33 0.014619392256011565 34-35 0.025052934425964537 36-37 0.03058571647040832 38-39 0.02142657915338903 40-41 0.0013365709433206887 42-43 5.180507532250731E-5 44-45 0.011220979314855085 46-47 0.018235386513522574 48-49 0.0033155248206404677 50-51 0.011169174239532577 52-53 0.010371376079565965 54-55 0.010329932019307958 56-57 5.284117682895747E-4 58-59 0.008278451036536669 60-61 0.004724622869412667 62-63 0.0013365709433206887 64-65 0.03458506828530589 66-67 0.05910959094298085 68-69 0.05718244214098357 70-71 0.05064464163528315 72-73 0.04437622752125977 74-75 0.03500986990295044 76-77 0.030969074027794873 78-79 0.01513744300923664 80-81 0.0012640438378691786 82-83 0.037486152503366295 84-85 0.050655002650347655 86-87 0.05649861514672648 88-89 0.06832053333532265 90-91 0.04770211335696474 92-93 0.038408282844106925 94-95 0.013085962026465349 96-97 0.0036367162876400137 98-99 0.021188275806905493 100 0.030917268952472363 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4825782.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.15687073033158 #Duplication Level Percentage of deduplicated Percentage of total 1 79.53738717337883 53.41482028630935 2 13.0996125240689 17.594579697926555 3 3.6265874401033398 7.306507917217922 4 1.440541144671508 3.8696894173771335 5 0.7012032598030128 2.354530833713901 6 0.4098674470518776 1.6515249094940379 7 0.25396013862349676 1.1938617740136679 8 0.16321298399826278 0.8768698614306408 9 0.1265200794424815 0.764702335791901 >10 0.6022574358270788 6.8891263892179015 >50 0.026347765540095186 1.2105053506847814 >100 0.011560699560626097 1.3837582268387545 >500 5.434084734069253E-4 0.25613648894324775 >1k 2.8981778701455503E-4 0.37011813540428157 >5k 3.622722337681556E-5 0.19531377052687784 >10k+ 7.245444675363111E-5 0.6679546051090473 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14991 0.31064395366388287 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12464 0.2582793835278925 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8028 0.1663564578756355 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 4.144406025800585E-5 5 0.0 0.0 0.0 0.0 4.144406025800585E-5 6 0.0 0.0 0.0 2.0722030129002926E-5 4.144406025800585E-5 7 0.0 0.0 0.0 2.0722030129002926E-5 4.144406025800585E-5 8 0.0 0.0 0.0 2.0722030129002926E-5 4.144406025800585E-5 9 0.0 0.0 0.0 4.144406025800585E-5 4.144406025800585E-5 10-11 0.0 0.0 0.0 5.1805075322507315E-5 4.144406025800585E-5 12-13 0.0 0.0 0.0 8.28881205160117E-5 4.144406025800585E-5 14-15 0.0 0.0 0.0 8.28881205160117E-5 5.1805075322507315E-5 16-17 0.0 0.0 0.0 1.4505421090302048E-4 6.216609038700878E-5 18-19 0.0 0.0 0.0 2.0722030129002926E-4 8.28881205160117E-5 20-21 0.0 0.0 0.0 2.6938639167703806E-4 8.28881205160117E-5 22-23 0.0 0.0 0.0 5.698558285475805E-4 1.0361015064501463E-4 24-25 0.0 0.0 0.0 0.0012847658679981814 1.4505421090302048E-4 26-27 0.0 0.0 0.0 0.002528087675738357 1.4505421090302048E-4 28-29 0.0 0.0 0.0 0.006651771671409939 1.5541522596752193E-4 30-31 0.0 0.0 0.0 0.021571633364292048 1.657762410320234E-4 32-33 0.0 0.0 0.0 0.05477868664601924 1.657762410320234E-4 34-35 0.0 0.0 0.0 0.10251188304817747 1.7613725609652488E-4 36-37 0.0 0.0 0.0 0.17971180629377786 1.8649827116102633E-4 38-39 0.0 0.0 0.0 0.29481232264532464 1.8649827116102633E-4 40-41 0.0 0.0 0.0 0.42305060609865924 1.8649827116102633E-4 42-43 0.0 0.0 0.0 0.5423991386266516 1.8649827116102633E-4 44-45 0.0 0.0 0.0 0.6793199527040384 1.9685928622552778E-4 46-47 0.0 0.0 0.0 0.8408481775596162 2.486643615480351E-4 48-49 0.0 0.0 0.0 1.0158249999689168 2.486643615480351E-4 50-51 0.0 0.0 0.0 1.197318900853789 2.486643615480351E-4 52-53 0.0 0.0 0.0 1.3737669874022491 2.7974740674153954E-4 54-55 0.0 0.0 0.0 1.5687923739613598 3.108304519350439E-4 56-57 0.0 0.0 0.0 1.786498022496665 3.2119146699954534E-4 58-59 0.0 0.0 0.0 2.0467252768566837 3.315524820640468E-4 60-61 0.0 0.0 0.0 2.29619779757975 3.419134971285483E-4 62-63 0.0 0.0 0.0 2.550270608991455 3.937185724510556E-4 64-65 0.0 0.0 0.0 2.8098762024476036 3.937185724510556E-4 66-67 0.0 0.0 0.0 3.101238307076449 3.937185724510556E-4 68-69 0.0 0.0 0.0 3.420803094710868 4.144406025800585E-4 70-71 0.0 0.0 0.0 3.761297132775579 4.3516263270906147E-4 72-73 0.0 0.0 0.0 4.085368961963056 4.5588466283806437E-4 74-75 0.0 0.0 0.0 4.40646717982702 4.5588466283806437E-4 76-77 0.0 0.0 0.0 4.76300006921158 4.5588466283806437E-4 78-79 0.0 0.0 0.0 5.15590012147254 4.5588466283806437E-4 80-81 0.0 0.0 0.0 5.565004801294381 4.5588466283806437E-4 82-83 0.0 1.0361015064501463E-5 0.0 5.9770105653342815 4.5588466283806437E-4 84-85 0.0 2.0722030129002926E-5 0.0 6.3873067618885395 4.5588466283806437E-4 86-87 0.0 2.0722030129002926E-5 0.0 6.813517477581872 4.6624567790256585E-4 88 0.0 2.0722030129002926E-5 0.0 7.16010379250451 4.766066929670673E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7910 0.0 48.094955 1 GTATCAA 14020 0.0 41.926586 1 TACCTGG 3980 0.0 38.96904 2 ATCAACG 17235 0.0 33.436253 3 CAACGCA 17390 0.0 33.07251 5 ACCTGGG 4790 0.0 31.99046 3 TATCAAC 18410 0.0 31.936872 2 GTGGTAT 3125 0.0 31.600704 1 GTACTAG 2880 0.0 31.513187 1 GTACCTG 5075 0.0 30.855719 1 TCAACGC 18890 0.0 30.705881 4 AACGCAG 19235 0.0 30.437654 6 GTACATG 19345 0.0 27.857578 1 ACGCAGA 21100 0.0 27.279661 7 TACATGG 19410 0.0 27.095264 2 CGCAGAG 21570 0.0 26.68525 8 TAGGCAT 3835 0.0 26.220064 5 ACATGGG 19470 0.0 26.121595 3 TGGTATC 3690 0.0 25.091621 2 TGGGGGG 10925 0.0 24.902472 6 >>END_MODULE