FastQCFastQC Report
Mon 6 Jun 2016
ERR1211254_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211254_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4686733
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187220.39946803028890276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148160.3161263933746599No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91630.1955093238723008No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51920.11078079335861463No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84500.050.740671
TACCTGG45300.046.441742
GTATCAA152050.041.1060871
GTACCTG56550.037.951311
ACCTGGG56050.037.1561663
GTGGTAT34600.035.7745481
ATCAACG184850.032.9855163
CAACGCA187450.032.477495
TATCAAC192000.032.1856772
TCAACGC199850.030.7681714
AACGCAG202100.030.4718276
CCTGGGG51950.029.7720324
GTACTAG24200.028.5888371
GTACATG176150.028.4017491
ACGCAGA221200.027.4581267
TACATGG178500.027.368962
TGGTATC43300.026.9564632
CGCAGAG226450.026.717748
ACATGGG178950.026.50713
CTGGGGG41600.025.8785725
Produced by FastQC (version 0.11.5)