FastQCFastQC Report
Mon 6 Jun 2016
ERR1211254_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211254_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4686733
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170970.3647956903028186No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144610.30855182063923847No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92030.19636279685657365No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82400.050.7928281
TACCTGG45550.046.228172
GTATCAA148400.041.7658271
GTACCTG55350.039.0822681
ACCTGGG55550.037.1491173
TAACGCA6300.035.0691574
ATCAACG181900.033.1301463
CAACGCA184500.032.6754955
TATCAAC190000.032.3077432
ATAACGC7050.031.3383963
TCAACGC198100.030.6818874
AACGCAG202150.030.3804176
GTGGTAT31150.028.9856321
CCTGGGG50800.028.5932034
GTACATG171200.028.3750151
TACATGG175850.027.2096182
ACGCAGA221550.027.1687267
ACATGGG172500.026.8692683
CTGGGGG33250.026.7092725
CGCAGAG225450.026.6779028
Produced by FastQC (version 0.11.5)