FastQCFastQC Report
Mon 6 Jun 2016
ERR1211253_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211253_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9676151
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT322920.3337277394699607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247140.25541147507929546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153180.1583067482101096No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA173050.048.295051
GTATCAA310300.041.1584851
CAACGCA358050.034.201215
ATCAACG366550.033.420763
GTACTAG75800.033.2756771
TATCAAC391800.032.4284442
AACGCAG402450.031.1289866
GTGGTAT56350.030.3140531
GCATCGT77800.029.7250468
TCAACGC419400.029.4109044
TACCTGG58700.028.5389962
TAGTACT107350.028.3318654
CTGTGCG106750.028.312779
ACGCAGA435500.028.0435477
TAGGCAT98150.027.922595
CGCAGAG443100.027.4642268
GTACATG423700.027.3217141
TCTAACG38100.026.9251632
TACATGG426500.026.8110622
CTAACGC39750.026.7267743
Produced by FastQC (version 0.11.5)