##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211253_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9676151 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.48836949733422 31.0 31.0 34.0 30.0 34.0 2 31.507033323477486 31.0 31.0 34.0 28.0 34.0 3 31.569085682933224 33.0 31.0 34.0 28.0 34.0 4 35.1652520718207 37.0 35.0 37.0 32.0 37.0 5 35.22074655511267 37.0 35.0 37.0 32.0 37.0 6 35.2870871899374 37.0 35.0 37.0 32.0 37.0 7 35.20283199383722 37.0 35.0 37.0 32.0 37.0 8 35.20877319917806 37.0 35.0 37.0 32.0 37.0 9 36.773612565574886 39.0 37.0 39.0 32.0 39.0 10-11 36.72472737351866 39.0 37.0 39.0 32.0 39.0 12-13 36.61402757150027 39.0 37.0 39.0 32.0 39.0 14-15 37.806952320194256 40.0 37.0 41.0 32.0 41.0 16-17 37.78578837804412 40.0 37.0 41.0 32.0 41.0 18-19 37.797178340850614 40.0 37.0 41.0 32.0 41.0 20-21 37.76438167407681 40.0 37.0 41.0 32.0 41.0 22-23 37.65415990304409 40.0 37.0 41.0 32.0 41.0 24-25 37.553219043398556 40.0 37.0 41.0 32.0 41.0 26-27 37.400974881437875 40.0 37.0 41.0 31.0 41.0 28-29 37.28216782685595 40.0 36.0 41.0 31.0 41.0 30-31 37.13105071427678 39.5 36.0 41.0 31.0 41.0 32-33 36.94816167089579 39.0 36.0 41.0 30.0 41.0 34-35 36.803182587787234 39.0 35.0 41.0 30.0 41.0 36-37 36.69839670753382 39.0 35.0 41.0 30.0 41.0 38-39 36.55567074139293 39.0 35.0 41.0 30.0 41.0 40-41 36.380286438274894 39.0 35.0 40.5 29.5 41.0 42-43 36.164605120362424 39.0 35.0 40.0 28.5 41.0 44-45 35.926515925598935 38.0 35.0 40.0 28.0 41.0 46-47 35.67157597065197 38.0 34.0 40.0 27.0 41.0 48-49 35.450452664494385 38.0 34.0 40.0 26.0 41.0 50-51 34.74761612339452 37.5 33.5 39.5 25.5 40.5 52-53 34.84102382238558 38.0 33.5 39.5 26.0 40.5 54-55 35.3088414494565 38.0 34.0 40.0 26.0 41.0 56-57 35.244647329294466 38.0 34.0 40.0 26.0 41.0 58-59 35.006708865953 37.0 34.0 40.0 26.0 41.0 60-61 34.76911635628671 37.0 34.0 40.0 26.0 41.0 62-63 34.4550323263868 36.0 33.0 40.0 26.0 41.0 64-65 34.197118616689636 36.0 33.0 39.0 26.0 41.0 66-67 34.017100084527414 35.5 33.0 39.0 26.0 41.0 68-69 33.7213939716319 35.0 33.0 39.0 25.0 41.0 70-71 33.433216420454784 35.0 33.0 38.0 25.0 40.5 72-73 33.114940692843675 35.0 32.0 37.0 24.0 40.0 74-75 32.79999195961287 35.0 32.0 37.0 24.0 39.0 76-77 32.49645933594877 35.0 32.0 36.0 23.5 39.0 78-79 32.183735661008186 35.0 31.5 36.0 23.0 39.0 80-81 31.914790343805095 35.0 31.0 35.5 22.5 37.5 82-83 31.64746953618231 35.0 31.0 35.0 21.5 37.0 84-85 31.42516750720405 34.5 31.0 35.0 20.5 37.0 86-87 31.218712223486385 34.0 31.0 35.0 20.0 36.0 88-89 31.028444471360565 34.0 31.0 35.0 20.0 36.0 90-91 30.814467963552865 34.0 30.0 35.0 19.5 36.0 92-93 30.63037239704093 34.0 30.0 35.0 19.0 35.0 94-95 30.47643825525253 34.0 30.0 35.0 19.0 35.0 96-97 30.301755160703877 34.0 29.0 35.0 18.0 35.0 98-99 30.079494522150387 34.0 29.0 35.0 18.0 35.0 100 28.929893921663687 33.0 27.0 35.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.5362410028373148 1101 2 -0.5151641134286429 1101 3 -0.5684962082335119 1101 4 -0.4996221259681093 1101 5 -0.45131983733800496 1101 6 -0.4336318359693294 1101 7 -0.44852644924505825 1101 8 -0.40304839844977636 1101 9 -0.6769416546637288 1101 10-11 -0.6750818220882309 1101 12-13 -0.6145453939172114 1101 14-15 -0.8245922121218641 1101 16-17 -0.7988608277941864 1101 18-19 -0.8611465993766814 1101 20-21 -0.8733756013787399 1101 22-23 -0.9810460977197337 1101 24-25 -1.018255873427698 1101 26-27 -1.0123591770078235 1101 28-29 -0.8939034754788082 1101 30-31 -0.9791097895336165 1101 32-33 -1.099720611472108 1101 34-35 -1.1317736207704314 1101 36-37 -1.1281969141098145 1101 38-39 -1.1402804807447708 1101 40-41 -1.1774445499806419 1101 42-43 -1.2191628622147377 1101 44-45 -1.1836445894064127 1101 46-47 -1.323318030867334 1101 48-49 -1.3282221687784457 1101 50-51 -1.374582106852941 1101 52-53 -1.2464354087182485 1101 54-55 -1.21702889955926 1101 56-57 -1.062557778008454 1101 58-59 -1.0215237553350747 1101 60-61 -0.964452469246659 1101 62-63 -0.9950771498587798 1101 64-65 -1.0713690377158684 1101 66-67 -1.1013634628738984 1101 68-69 -1.0405519823863187 1101 70-71 -1.0865015721148907 1101 72-73 -1.046727687845248 1101 74-75 -1.1208551382953047 1101 76-77 -1.1910766475988481 1101 78-79 -1.1627325280613547 1101 80-81 -1.1523589007962194 1101 82-83 -1.172830109812864 1101 84-85 -1.312401140223205 1101 86-87 -1.3911730648563392 1101 88-89 -1.3910671967137098 1101 90-91 -1.4406751135089415 1101 92-93 -1.5684269940813955 1101 94-95 -1.5775020996697755 1101 96-97 -1.6661445345962385 1101 98-99 -1.7535568446122092 1101 100 -1.6645841754223518 1102 1 -0.46314492762905246 1102 2 -0.40223111037445136 1102 3 -0.43699139610196625 1102 4 -0.25906205930347426 1102 5 -0.2942420340240517 1102 6 -0.3557509875933249 1102 7 -0.35700158445149555 1102 8 -0.4274947242846068 1102 9 -0.5775993591476123 1102 10-11 -0.551115834244122 1102 12-13 -0.5373289722963648 1102 14-15 -0.7171664298016935 1102 16-17 -0.7271619449595761 1102 18-19 -0.7782371432972113 1102 20-21 -0.8010477485990251 1102 22-23 -0.7360308521158387 1102 24-25 -0.7268960086848679 1102 26-27 -0.7854326184555731 1102 28-29 -0.8979491158956421 1102 30-31 -0.8380145983674367 1102 32-33 -0.8970278725397591 1102 34-35 -0.917426752841493 1102 36-37 -0.9400929751125204 1102 38-39 -1.0573679806179328 1102 40-41 -1.0925426761158192 1102 42-43 -1.0278320364154112 1102 44-45 -1.207160318252825 1102 46-47 -1.1590036644857875 1102 48-49 -1.1029155000316777 1102 50-51 -1.0389786878804088 1102 52-53 -1.035095818599224 1102 54-55 -0.8574143013227769 1102 56-57 -0.9055674466638948 1102 58-59 -0.8699235250021573 1102 60-61 -0.836835203663675 1102 62-63 -0.9668591922623548 1102 64-65 -0.9487291383723715 1102 66-67 -0.9243624456547295 1102 68-69 -0.9999894487749614 1102 70-71 -1.0314088618473178 1102 72-73 -0.9906071613265226 1102 74-75 -1.073732796763334 1102 76-77 -1.1244832663725006 1102 78-79 -1.0824745133383118 1102 80-81 -1.2174364098522616 1102 82-83 -1.1992458391666112 1102 84-85 -1.199721129213902 1102 86-87 -1.2366818076140405 1102 88-89 -1.4043081495512695 1102 90-91 -1.3914369688050776 1102 92-93 -1.384098219208994 1102 94-95 -1.3876293535331783 1102 96-97 -1.4078469624776844 1102 98-99 -1.5877985724134938 1102 100 -1.5995661140746513 1103 1 -0.5794628619776248 1103 2 -0.4180151990536167 1103 3 -0.5265668477806749 1103 4 -0.39947974706749534 1103 5 -0.39457417018253693 1103 6 -0.42964043678641417 1103 7 -0.41757406829267296 1103 8 -0.4421944494059531 1103 9 -0.6157413194415682 1103 10-11 -0.5631918449593698 1103 12-13 -0.6321160440249329 1103 14-15 -0.7451341684494466 1103 16-17 -0.8207175542637657 1103 18-19 -0.7363147573444664 1103 20-21 -0.8024591979716078 1103 22-23 -0.8751001285324094 1103 24-25 -0.8589430864069243 1103 26-27 -0.8829322115378133 1103 28-29 -0.8665911667002746 1103 30-31 -0.8811957064311002 1103 32-33 -0.9629139872890349 1103 34-35 -0.9267737829503631 1103 36-37 -0.8671984740545398 1103 38-39 -0.9391537639003857 1103 40-41 -0.9547493720805633 1103 42-43 -1.1322650212446135 1103 44-45 -1.10601401192514 1103 46-47 -1.0611099924751883 1103 48-49 -1.1766196989665474 1103 50-51 -1.293916935259027 1103 52-53 -1.1157697656710113 1103 54-55 -0.9438885377244972 1103 56-57 -0.8376252335485219 1103 58-59 -0.8475023415484273 1103 60-61 -0.8126207222594317 1103 62-63 -0.8297333246148284 1103 64-65 -0.7939719427734673 1103 66-67 -0.868475068319519 1103 68-69 -0.8583400856202559 1103 70-71 -0.818924868546631 1103 72-73 -0.8693915801034606 1103 74-75 -0.8499974591128705 1103 76-77 -0.8505116730346955 1103 78-79 -0.9196291922840061 1103 80-81 -0.9288529552041922 1103 82-83 -0.8394682064087426 1103 84-85 -0.9389270083007624 1103 86-87 -1.0389938002028138 1103 88-89 -1.0473049200694327 1103 90-91 -1.1704514824584997 1103 92-93 -1.2988844999198754 1103 94-95 -1.36964757432143 1103 96-97 -1.353927576307413 1103 98-99 -1.3359911730514789 1103 100 -1.3767090494500245 1104 1 -0.5123965458901232 1104 2 -0.4561638649468236 1104 3 -0.5234902978133604 1104 4 -0.341095162510328 1104 5 -0.3637834217221041 1104 6 -0.3617368994583856 1104 7 -0.3442750915207 1104 8 -0.3236614580878694 1104 9 -0.47388832915213897 1104 10-11 -0.5429420222361259 1104 12-13 -0.5180410878565596 1104 14-15 -0.7663843328680215 1104 16-17 -0.8126641877772514 1104 18-19 -0.7037288656334724 1104 20-21 -0.7456411087166472 1104 22-23 -0.6640419287241883 1104 24-25 -0.7732919648476724 1104 26-27 -0.7641728916653392 1104 28-29 -0.8173347570212286 1104 30-31 -0.7515813767353379 1104 32-33 -0.8623925173912639 1104 34-35 -1.0499506070245204 1104 36-37 -0.9654868228342934 1104 38-39 -0.9094520662639738 1104 40-41 -0.966346304968134 1104 42-43 -0.9834758433276534 1104 44-45 -0.9159216262186618 1104 46-47 -1.0789074098392462 1104 48-49 -1.135379514709463 1104 50-51 -1.0637755432805776 1104 52-53 -0.9548287721607593 1104 54-55 -0.8106878220468872 1104 56-57 -0.8571145719035513 1104 58-59 -0.8913606786253965 1104 60-61 -0.9568277989826441 1104 62-63 -0.8580129445984284 1104 64-65 -0.825687409612101 1104 66-67 -0.8668830350747925 1104 68-69 -1.0155927813158954 1104 70-71 -0.9274730411287351 1104 72-73 -0.8754934749560448 1104 74-75 -0.9118488547420505 1104 76-77 -0.921499881285019 1104 78-79 -0.9128597057141619 1104 80-81 -0.9904641981987936 1104 82-83 -0.9816179253191137 1104 84-85 -1.03502532008957 1104 86-87 -0.9914088430168597 1104 88-89 -1.12440033255427 1104 90-91 -1.1338225733106988 1104 92-93 -1.171766576393921 1104 94-95 -1.2122578067493102 1104 96-97 -1.3493747536172656 1104 98-99 -1.3845173304629022 1104 100 -1.4348902286938987 1105 1 -0.5524215350585244 1105 2 -0.5223965264419093 1105 3 -0.5815298006943301 1105 4 -0.3880071741079618 1105 5 -0.3614649882554559 1105 6 -0.37540817490250333 1105 7 -0.3650590739960293 1105 8 -0.4284890309213978 1105 9 -0.6359247905531191 1105 10-11 -0.6169169307187872 1105 12-13 -0.5907816627078475 1105 14-15 -0.7899186276894099 1105 16-17 -0.7825295489615343 1105 18-19 -0.8480890681517863 1105 20-21 -0.8466818450531974 1105 22-23 -0.8326594478497498 1105 24-25 -0.7819459781219109 1105 26-27 -0.7964105810210711 1105 28-29 -0.8209518199297392 1105 30-31 -0.8967815368033385 1105 32-33 -0.949923286689959 1105 34-35 -0.8168990457419341 1105 36-37 -0.894385735499263 1105 38-39 -0.9334213873553665 1105 40-41 -0.9172285228839598 1105 42-43 -0.9755088207094573 1105 44-45 -1.115774870010938 1105 46-47 -1.0566069539709488 1105 48-49 -1.0959964262711495 1105 50-51 -1.0720386741385468 1105 52-53 -0.9464577705185988 1105 54-55 -0.8602344654728995 1105 56-57 -0.8069074525777253 1105 58-59 -0.8087606955483366 1105 60-61 -0.8528398992587825 1105 62-63 -0.8292708066132164 1105 64-65 -0.8653394360852076 1105 66-67 -0.7937022521984218 1105 68-69 -0.8861656363345674 1105 70-71 -0.8545741259409141 1105 72-73 -0.7795136252308978 1105 74-75 -0.8333230544125598 1105 76-77 -0.820813730345364 1105 78-79 -0.8893336782036663 1105 80-81 -0.9439614401323659 1105 82-83 -1.0000746257699156 1105 84-85 -1.010534911072991 1105 86-87 -1.0239082171869889 1105 88-89 -1.0572327414616716 1105 90-91 -1.0996615562204788 1105 92-93 -1.0431919228733975 1105 94-95 -1.1356994780080392 1105 96-97 -1.167432975866678 1105 98-99 -1.1674293034261645 1105 100 -1.2569429472021412 1106 1 -0.5719846446805192 1106 2 -0.5634279438724512 1106 3 -0.6351344705911401 1106 4 -0.466334830659477 1106 5 -0.45248912301221367 1106 6 -0.4618475343847024 1106 7 -0.5060496335734896 1106 8 -0.47372929031502053 1106 9 -0.6076629437684957 1106 10-11 -0.5932547687622645 1106 12-13 -0.6758706478026397 1106 14-15 -0.9094213804917857 1106 16-17 -0.8923557901622416 1106 18-19 -0.9185019377837094 1106 20-21 -0.7911057263777366 1106 22-23 -0.8425864628979483 1106 24-25 -0.9071178274838516 1106 26-27 -0.9532894106144738 1106 28-29 -0.951150531850594 1106 30-31 -1.046683715880782 1106 32-33 -0.9422722702436772 1106 34-35 -1.0335879147004192 1106 36-37 -0.9926524531003622 1106 38-39 -0.9253361822460491 1106 40-41 -1.0559351490059825 1106 42-43 -1.0061576211938998 1106 44-45 -1.0669315590924526 1106 46-47 -1.0540774929588537 1106 48-49 -1.017701227739181 1106 50-51 -1.0709049260833083 1106 52-53 -1.0143377129238829 1106 54-55 -0.8743453886864714 1106 56-57 -0.798277248636289 1106 58-59 -0.8487334803292654 1106 60-61 -0.8908123944235982 1106 62-63 -0.938024991131293 1106 64-65 -0.9501687026752705 1106 66-67 -1.0238143389489522 1106 68-69 -0.9976679130457384 1106 70-71 -0.9471307200457346 1106 72-73 -0.9834486483216835 1106 74-75 -0.9308612449712683 1106 76-77 -0.8577247788257516 1106 78-79 -0.8847874721143825 1106 80-81 -0.8491260696230185 1106 82-83 -0.9484571837782845 1106 84-85 -0.9461947285641124 1106 86-87 -1.0282481774716565 1106 88-89 -1.1310427549811166 1106 90-91 -1.1763988422783491 1106 92-93 -1.2737588385680567 1106 94-95 -1.3993932054640545 1106 96-97 -1.3758924476059171 1106 98-99 -1.2474317333995693 1106 100 -1.3386551992198505 1107 1 -0.6551353862316986 1107 2 -0.6093243698389017 1107 3 -0.5783530458403483 1107 4 -0.5160168146127972 1107 5 -0.455459947228114 1107 6 -0.4787383683258639 1107 7 -0.5163518460704495 1107 8 -0.49234052299189557 1107 9 -0.6459089928810684 1107 10-11 -0.6198779148843201 1107 12-13 -0.7290325734043961 1107 14-15 -0.9617724624271133 1107 16-17 -0.8795152844486438 1107 18-19 -0.9274178142728928 1107 20-21 -0.8840494414275852 1107 22-23 -0.9455473187209478 1107 24-25 -0.8610040419258667 1107 26-27 -0.9084106116842605 1107 28-29 -1.01935585643119 1107 30-31 -1.0051200154188322 1107 32-33 -1.0529135296650267 1107 34-35 -0.9919578393296433 1107 36-37 -1.0105375734504705 1107 38-39 -0.9771402182295148 1107 40-41 -1.0295255939366896 1107 42-43 -0.9995833445102136 1107 44-45 -0.9624741071109355 1107 46-47 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68-69 1.1834608778974243 2311 70-71 1.1466834207875252 2311 72-73 1.1473542055768462 2311 74-75 1.1908580144576604 2311 76-77 1.1686180545067728 2311 78-79 1.1898803040768087 2311 80-81 1.1869457505657834 2311 82-83 1.240983294210725 2311 84-85 1.2456987327138016 2311 86-87 1.2552875425577774 2311 88-89 1.3233886096365843 2311 90-91 1.3369597761930443 2311 92-93 1.3807525726399277 2311 94-95 1.4162826947859521 2311 96-97 1.4542011715721266 2311 98-99 1.5486758336226956 2311 100 1.5995985423795211 2312 1 0.6154551684218212 2312 2 0.6199874348396754 2312 3 0.6591776134496463 2312 4 0.3659776528837426 2312 5 0.47890634058233417 2312 6 0.5231013494362813 2312 7 0.38373088089248597 2312 8 0.5065760685048843 2312 9 0.7217056963165192 2312 10-11 0.7189322761474131 2312 12-13 0.7890000803931869 2312 14-15 0.996231727041085 2312 16-17 1.0005183515687577 2312 18-19 0.9793896505977173 2312 20-21 0.9939257324654207 2312 22-23 1.0679090326331035 2312 24-25 1.0545469232554439 2312 26-27 1.091073953432911 2312 28-29 1.1272128187747725 2312 30-31 1.123790853144115 2312 32-33 1.114747534339628 2312 34-35 1.1580526744314596 2312 36-37 1.24287240309652 2312 38-39 1.231554030773232 2312 40-41 1.2614033554890156 2312 42-43 1.3309213962037987 2312 44-45 1.3374814624763331 2312 46-47 1.3852530582005542 2312 48-49 1.4048559648640193 2312 50-51 1.4054935495701315 2312 52-53 1.2855774520228564 2312 54-55 1.181751010620168 2312 56-57 1.1256037892489346 2312 58-59 1.1131689881773426 2312 60-61 1.0515116419248116 2312 62-63 1.0784591623992341 2312 64-65 1.0603368593697908 2312 66-67 1.0684333892757039 2312 68-69 1.070558615889965 2312 70-71 1.0515095603797135 2312 72-73 1.0439442706160378 2312 74-75 1.0476773419507097 2312 76-77 1.0347388465167597 2312 78-79 1.0529270087831222 2312 80-81 1.0889741561432373 2312 82-83 1.153584857931822 2312 84-85 1.1458406159534427 2312 86-87 1.130803108851186 2312 88-89 1.1592716893611303 2312 90-91 1.1506822022389365 2312 92-93 1.2444681640859514 2312 94-95 1.2961535365185597 2312 96-97 1.3130799034304026 2312 98-99 0.9787322220851209 2312 100 1.2713488629146212 2313 1 0.6555280124611151 2313 2 0.6621616245588591 2313 3 0.6829784200793547 2313 4 0.5628711405692783 2313 5 0.4232088202788873 2313 6 0.5373671405443545 2313 7 0.5978671081779154 2313 8 0.5946958915739273 2313 9 0.7624437148686951 2313 10-11 0.747644960932675 2313 12-13 0.8111407459233391 2313 14-15 1.0299548951825912 2313 16-17 1.0235171618632108 2313 18-19 0.985508889742043 2313 20-21 1.0433887004958535 2313 22-23 1.0881604310456154 2313 24-25 1.081628311015308 2313 26-27 1.0792394816864288 2313 28-29 1.1513083592550828 2313 30-31 1.1404327390162692 2313 32-33 1.172008272389128 2313 34-35 1.1544524081026566 2313 36-37 1.190885045597149 2313 38-39 1.2263430917611515 2313 40-41 1.1977170531221333 2313 42-43 1.2478734715495463 2313 44-45 1.2634909955968112 2313 46-47 1.2194596571309475 2313 48-49 1.258424028161869 2313 50-51 1.3028210659546389 2313 52-53 1.2220923870154294 2313 54-55 1.0632716822119122 2313 56-57 0.9994708796084453 2313 58-59 1.0347410582826768 2313 60-61 1.073881133425182 2313 62-63 1.0196017986437056 2313 64-65 1.0288248860074702 2313 66-67 0.9863196873092548 2313 68-69 1.055302224681128 2313 70-71 1.0660450234464065 2313 72-73 1.0663843839065237 2313 74-75 1.025089297171192 2313 76-77 0.78120842027446 2313 78-79 0.9719905919558798 2313 80-81 1.0514865797036776 2313 82-83 1.1610587341862733 2313 84-85 1.1366804154759969 2313 86-87 1.1555941997418095 2313 88-89 1.2115844408643959 2313 90-91 1.204545474726352 2313 92-93 1.2648221709692393 2313 94-95 1.307503825777328 2313 96-97 1.4116000247659635 2313 98-99 1.4828045268594572 2313 100 1.648474701974763 2314 1 0.5662271568217143 2314 2 0.5849096885044212 2314 3 0.6267013965108923 2314 4 0.4919456218774698 2314 5 0.4622440545986777 2314 6 0.5049535500010975 2314 7 0.5436464603366602 2314 8 0.525381233016553 2314 9 0.669361163728702 2314 10-11 0.6712431294345294 2314 12-13 0.7278461729562835 2314 14-15 0.8978917685104548 2314 16-17 0.912088567047995 2314 18-19 0.8521409381771079 2314 20-21 0.8527009058541068 2314 22-23 0.8938892476621803 2314 24-25 0.9160514470277192 2314 26-27 0.9314642622477294 2314 28-29 0.9311440293940052 2314 30-31 1.0422698439558715 2314 32-33 1.0247081616443694 2314 34-35 0.9931992176245501 2314 36-37 1.0333328666933923 2314 38-39 1.0614887326943503 2314 40-41 1.1018523532003783 2314 42-43 1.1405323526220954 2314 44-45 1.155618707488216 2314 46-47 1.186244834123471 2314 48-49 1.1479653569814587 2314 50-51 1.0955601827094128 2314 52-53 1.0960152745897105 2314 54-55 0.9679335341080062 2314 56-57 0.9190263891302877 2314 58-59 0.9199579340379458 2314 60-61 0.9031641321615851 2314 62-63 0.8890090098498149 2314 64-65 0.9207845813430424 2314 66-67 0.8774236862816807 2314 68-69 0.8734910261479314 2314 70-71 0.8710973588607942 2314 72-73 0.9041590135819959 2314 74-75 0.9255280906025902 2314 76-77 0.900454256132349 2314 78-79 0.9019141209238981 2314 80-81 0.8937003394583058 2314 82-83 0.9467910396105843 2314 84-85 1.003928724501609 2314 86-87 1.0940137446448865 2314 88-89 1.1251620240213107 2314 90-91 1.1706260765594827 2314 92-93 1.2709310400606313 2314 94-95 1.281078940443848 2314 96-97 1.2816308348721641 2314 98-99 1.3424024495616784 2314 100 1.3810815891379171 2315 1 0.470021821653301 2315 2 0.4585052301383854 2315 3 0.5425168781676355 2315 4 0.24015619733962268 2315 5 0.3194796944832632 2315 6 0.38989089853575365 2315 7 0.4251082699522257 2315 8 0.43344967091549336 2315 9 0.6196502345176924 2315 10-11 0.48568273204145385 2315 12-13 0.4991920241596972 2315 14-15 0.7930570996961208 2315 16-17 0.7707838193566303 2315 18-19 0.6190589007523286 2315 20-21 0.7829378727326741 2315 22-23 0.5985369349390623 2315 24-25 0.7510147810023398 2315 26-27 0.8755981846514729 2315 28-29 0.881554727562218 2315 30-31 0.9012522933945348 2315 32-33 0.9429755437623797 2315 34-35 0.9701122237919861 2315 36-37 0.9807477088934533 2315 38-39 1.0530300303769877 2315 40-41 1.0478983194153741 2315 42-43 1.137554131949294 2315 44-45 1.1545882716377989 2315 46-47 1.1670399391372825 2315 48-49 1.174392222675003 2315 50-51 1.1280579696548827 2315 52-53 1.0711637009809323 2315 54-55 0.9483167295298784 2315 56-57 0.9471997981710132 2315 58-59 0.9381927783796229 2315 60-61 0.9064514299834627 2315 62-63 0.9508354927217937 2315 64-65 0.93571697594966 2315 66-67 0.951247610825142 2315 68-69 0.9449392182231122 2315 70-71 0.8967839214031699 2315 72-73 0.7025918068936079 2315 74-75 0.9058509664586083 2315 76-77 0.9216806533717659 2315 78-79 0.9896714458109273 2315 80-81 0.7115051145215716 2315 82-83 0.6575208762358393 2315 84-85 0.9506139774665066 2315 86-87 1.0960244345220325 2315 88-89 1.125211399930965 2315 90-91 1.1852812294447297 2315 92-93 1.1910800053567208 2315 94-95 1.2621051823322134 2315 96-97 1.1110570981796073 2315 98-99 0.5791830562330524 2315 100 1.0903841301272514 2316 1 0.3609808910448855 2316 2 0.30290371251131987 2316 3 0.3843371384452574 2316 4 0.2455971109018904 2316 5 0.1494149081963556 2316 6 0.23281502823516576 2316 7 0.20405443043506466 2316 8 0.2536305619028525 2316 9 0.3633517828148243 2316 10-11 0.2972581788777262 2316 12-13 0.036683942903721345 2316 14-15 0.14537901417092058 2316 16-17 0.2819972454302899 2316 18-19 0.385910931197067 2316 20-21 0.4058732588577172 2316 22-23 0.43119830545978033 2316 24-25 0.454489018448875 2316 26-27 0.5153663179170991 2316 28-29 0.4532554088938099 2316 30-31 0.37041437427967594 2316 32-33 0.5664703858257596 2316 34-35 0.5749493239057628 2316 36-37 0.5791782488951256 2316 38-39 0.7532503284587406 2316 40-41 0.7470710981752688 2316 42-43 0.732975449537129 2316 44-45 0.6941745936370012 2316 46-47 0.5851482642878949 2316 48-49 0.4928940956822814 2316 50-51 0.5483342738401618 2316 52-53 0.6036638002788592 2316 54-55 0.357763038782295 2316 56-57 0.18607514917886903 2316 58-59 0.23113706984482718 2316 60-61 0.33911796636099467 2316 62-63 0.43058795316796505 2316 64-65 0.42024732937760234 2316 66-67 0.38545657141751377 2316 68-69 0.31331596105700754 2316 70-71 0.11141990049646466 2316 72-73 0.10024898370203061 2316 74-75 0.33464566656142125 2316 76-77 0.450021122315718 2316 78-79 0.4333285752755103 2316 80-81 0.6201932131204018 2316 82-83 0.7019753090049612 2316 84-85 0.7093516378511389 2316 86-87 0.7634609064522486 2316 88-89 0.7321314478351262 2316 90-91 0.5939853198871781 2316 92-93 0.6209155495073304 2316 94-95 0.8311807410264258 2316 96-97 0.7755785403567934 2316 98-99 0.6603960917643938 2316 100 0.47231677350150036 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 29.0 1 604.0 2 214.0 3 209.0 4 166.0 5 161.0 6 161.0 7 666.0 8 2264.0 9 4493.0 10 6952.0 11 10046.0 12 14086.0 13 18015.0 14 21230.0 15 25071.0 16 30099.0 17 37747.0 18 45934.0 19 56721.0 20 70912.0 21 87768.0 22 97694.0 23 99963.0 24 97968.0 25 101136.0 26 110797.0 27 127158.0 28 151502.0 29 181841.0 30 220671.0 31 275470.0 32 351943.0 33 465712.0 34 667551.0 35 932608.0 36 1410954.0 37 1943662.0 38 1575378.0 39 430276.0 40 319.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.98173736202227 19.882525238936317 12.85896028125447 23.276777117786942 2 15.860495516369335 19.27821483469438 40.9855605298936 23.875729119042685 3 19.473733118348548 25.68705738590889 28.428196111358094 26.411013384384468 4 12.78861681304214 15.755959270039357 38.264222962744824 33.19120095417368 5 13.544457356111803 37.86376084550771 34.04878005416836 14.543001744212129 6 31.210930165035283 36.90907714743985 17.436024114623873 14.443968572900992 7 28.22626252181219 31.82035287274607 21.98672784851031 17.966656756931428 8 26.924287068346292 35.06153531030905 19.256286474019017 18.757891147325644 9 26.766859473183917 15.529333602695885 20.94244567275055 36.76136125136964 10-11 26.330002546953345 25.837877605735653 26.62486396131319 21.20725588599781 12-13 26.232862829175556 23.40966212129209 27.506128767732434 22.85134628179992 14-15 23.557917750526638 24.625970434868627 25.29160271524592 26.524509099358813 16-17 22.363794578094723 27.516407796399182 26.376517587761278 23.743280037744817 18-19 21.92520662594315 27.202842463064865 28.745212959135095 22.126737951856885 20-21 23.229838524406006 26.37505337046262 28.35500514801107 22.040102957120304 22-23 23.54722298029292 26.043935710091716 28.06630465345143 22.34253665616394 24-25 22.921262736528703 26.060990823719266 28.71263767664401 22.305108763108024 26-27 22.815278800604467 26.363150681653515 27.95449472269666 22.86707579504536 28-29 22.192526234494604 26.705012805274908 28.433452288987144 22.669008671243347 30-31 23.054879080216114 26.345492524264863 28.30727703821585 22.292351357303172 32-33 23.204329139964404 26.54712799186304 27.63262782391086 22.61591504426169 34-35 23.183295984608502 26.465271784784928 28.229407881308845 22.122024349297728 36-37 22.854282591458595 26.193428283748073 27.947514383120442 23.004774741672886 38-39 23.04182391594903 26.380310167425908 28.252705928078285 22.325159988546776 40-41 22.8174612199161 25.630968452869507 28.673476138593184 22.8780941886212 42-43 22.769984356643715 25.835930221450877 28.119000123641648 23.275085298263765 44-45 22.777458563535912 26.543090691738552 28.046049562211238 22.633401182514294 46-47 22.835506826686952 26.396662463796577 27.47767670084021 23.290154008676257 48-49 23.396257255974113 26.3674775623163 27.389950165361586 22.846315016347994 50-51 23.092609898402642 26.28463076369621 27.424815061384322 23.19794427651683 52-53 22.727694738744447 26.74373354319741 27.178475381016202 23.350096337041933 54-55 22.611276752811783 26.617225897159635 27.292681846337587 23.478815503691 56-57 23.329289379070804 26.32162943804337 26.89497871974394 23.454102463141886 58-59 22.664180652649502 26.635349727674555 27.492997681807612 23.20747193786833 60-61 22.71573656533313 27.1244266031337 27.031072590161926 23.12876424137124 62-63 22.97278906606921 26.58277500056468 26.789041156215177 23.65539477715094 64-65 22.417827229518824 26.610449745875904 27.138814303930797 23.832908720674475 66-67 22.804422074187983 26.335268690758483 27.001365906609205 23.858943328444326 68-69 22.41342875279921 26.43279110208656 27.245336721675535 23.9084434234387 70-71 22.455927641836894 26.127696240128557 27.3830028042169 24.033373313817645 72-73 22.928996384710103 26.293708274802146 27.355522944267523 23.421772396220224 74-75 23.07872060741438 26.508265650339773 27.050084519319118 23.362929222926734 76-77 22.824531156597374 26.348359897000044 26.829289596564216 23.997819349838366 78-79 22.67121187046779 26.368732096114762 26.94721759164841 24.012838441769045 80-81 22.363688369302935 26.742745926071642 27.304067875908995 23.589497828716432 82-83 23.09221322172324 25.986739927156428 28.089910695042615 22.831136156077715 84-85 23.13111393881149 26.320204578414646 27.120841606872037 23.427839875901828 86-87 22.24483210277333 26.85896699111334 27.43499868936884 23.461202216744493 88-89 22.913229092598282 25.8818097819003 27.429140622246255 23.775820503255158 90-91 22.8033113284113 25.93051453188498 27.470798775723715 23.795375363980003 92-93 22.961827186236107 26.10860118121928 27.397126038104698 23.532445594439917 94-95 22.136998473673675 26.03370735934692 27.899209846637053 23.930084320342356 96-97 22.383444348560662 25.952547037611485 27.557023399823937 24.10698521400392 98-99 21.963397886077253 26.316523982825217 27.427157844354955 24.292920286742575 100 22.5738756046548 25.865455468709296 26.81134894360635 24.749319983029554 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1736.0 1 1224.0 2 1374.5 3 1911.0 4 1457.0 5 1222.5 6 1769.0 7 3210.0 8 5332.0 9 6300.5 10 5528.0 11 4974.0 12 5401.5 13 6339.5 14 7441.5 15 8522.5 16 9633.5 17 10786.0 18 11863.0 19 12714.5 20 13492.0 21 14450.5 22 15700.5 23 17649.5 24 20199.5 25 23114.0 26 27511.5 27 32879.5 28 39584.0 29 52975.0 30 73343.5 31 93565.0 32 120966.5 33 150971.5 34 174954.5 35 199543.0 36 223935.0 37 261655.5 38 281312.0 39 272998.0 40 261822.5 41 248939.5 42 249463.5 43 264821.5 44 288233.5 45 331082.0 46 372234.0 47 401142.5 48 425829.0 49 430754.0 50 422881.0 51 433547.5 52 436380.5 53 419521.5 54 400763.0 55 357998.0 56 318691.0 57 272513.0 58 225030.0 59 205191.0 60 172565.0 61 130959.5 62 102334.0 63 83534.5 64 62489.0 65 38648.0 66 25589.0 67 18152.5 68 13601.0 69 10212.5 70 8014.5 71 6167.5 72 4613.0 73 3709.0 74 2924.5 75 2118.0 76 1490.5 77 967.0 78 609.0 79 445.5 80 341.0 81 243.5 82 192.5 83 147.5 84 115.5 85 94.0 86 72.5 87 55.5 88 50.0 89 45.0 90 32.0 91 21.0 92 17.0 93 15.0 94 13.0 95 12.5 96 12.0 97 8.0 98 5.5 99 3.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0566134199435292 2 0.13204630642907494 3 0.01723825930372521 4 0.016556169906815223 5 0.019315531557951092 6 0.05174578197467154 7 0.05710948495946373 8 0.04869704906424052 9 0.04064632724313624 10-11 0.03938032798371997 12-13 0.040692833338380106 14-15 0.01876262575894072 16-17 0.017677483536583917 18-19 0.01782216916623149 20-21 0.020943244891486294 22-23 0.025537013632796764 24-25 0.024302018436876398 26-27 0.019062331706067838 28-29 0.01878329513460466 30-31 0.01671635756821075 32-33 0.02342356997115899 34-35 0.02312386402403187 36-37 0.022989513082216265 38-39 0.020886404108410462 40-41 0.024503544849599806 42-43 0.03136577757002759 44-45 0.04096153522201131 46-47 0.047389711053496375 48-49 0.03991773175098239 50-51 0.027603951199190672 52-53 0.039881560343570495 54-55 0.03616624006797744 56-57 0.038109161380387724 58-59 0.04315248904238886 60-61 0.02297401105046831 62-63 0.02567653191852835 64-65 0.025139128151265934 66-67 0.031918683369037956 68-69 0.03897210781435717 70-71 0.04063599255530428 72-73 0.044568341275368684 74-75 0.027107886183256134 76-77 0.020380004404643954 78-79 0.019883939388709415 80-81 0.02459655704008753 82-83 0.02062803691261122 84-85 0.012711666033322548 86-87 0.01470626078489267 88-89 0.018747123727192765 90-91 0.020953579579318263 92-93 0.01973925375906184 94-95 0.023454574034654896 96-97 0.02483425486022283 98-99 0.02755227776003082 100 0.029588211262928824 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9676151.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.560622786686835 #Duplication Level Percentage of deduplicated Percentage of total 1 74.66876295388725 43.72649261291127 2 14.207516772400494 16.640020608881436 3 5.060418986704564 8.890238622689818 4 2.23931602373316 5.245429638640841 5 1.1773299339988827 3.447258708019174 6 0.6813799340400547 2.3941219975042314 7 0.4650279255948196 1.906262745522359 8 0.30157385265963726 1.4128282102343113 9 0.21757846886840312 1.1467377577716693 >10 0.9148632205427389 9.004485191064306 >50 0.042481270612254575 1.7171061625879918 >100 0.021899715626601018 2.33149562684904 >500 0.0012664671422760601 0.510091389955457 >1k 4.870618255473564E-4 0.5082307256089239 >5k 2.4353090845101107E-5 0.08776417353686457 >10k+ 7.305927253530333E-5 1.0314358282223257 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32292 0.3337277394699607 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24714 0.25541147507929546 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15318 0.1583067482101096 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.0669375663939102E-5 2 0.0 0.0 0.0 0.0 2.0669375663939102E-5 3 0.0 0.0 0.0 0.0 2.0669375663939102E-5 4 0.0 0.0 0.0 1.0334687831969551E-5 2.0669375663939102E-5 5 0.0 0.0 0.0 1.0334687831969551E-5 2.0669375663939102E-5 6 0.0 0.0 0.0 1.0334687831969551E-5 2.0669375663939102E-5 7 0.0 0.0 0.0 1.0334687831969551E-5 2.0669375663939102E-5 8 0.0 0.0 0.0 1.0334687831969551E-5 3.1004063495908655E-5 9 0.0 0.0 0.0 2.0669375663939102E-5 4.1338751327878204E-5 10-11 0.0 0.0 0.0 2.0669375663939102E-5 4.1338751327878204E-5 12-13 0.0 0.0 0.0 2.0669375663939102E-5 5.6840783075832535E-5 14-15 0.0 0.0 0.0 4.650609524386298E-5 7.751015873977163E-5 16-17 0.0 0.0 0.0 9.301219048772596E-5 8.267750265575641E-5 18-19 0.0 0.0 0.0 1.3951828573158893E-4 1.0851422223568028E-4 20-21 0.0 0.0 0.0 1.8602438097545192E-4 1.3951828573158893E-4 22-23 0.0 0.0 0.0 3.307100106230256E-4 1.5502031747954327E-4 24-25 0.0 0.0 0.0 7.647668995657468E-4 1.8602438097545192E-4 26-27 0.0 0.0 0.0 0.0014675256721396763 1.8602438097545192E-4 28-29 0.0 0.0 0.0 0.004449083111662892 1.911917248914367E-4 30-31 0.0 0.0 0.0 0.016757696319538627 1.9635906880742147E-4 32-33 0.0 0.0 0.0 0.04417562313775385 1.9635906880742147E-4 34-35 0.0 0.0 0.0 0.08501830944969752 2.0669375663939102E-4 36-37 0.0 0.0 0.0 0.15226095582840737 2.0669375663939102E-4 38-39 0.0 0.0 0.0 0.25259527264508375 2.118611005553758E-4 40-41 0.0 0.0 0.0 0.359786654838272 2.1702844447136056E-4 42-43 0.0 0.0 0.0 0.46273564767643666 2.1702844447136056E-4 44-45 0.0 0.0 0.0 0.5839150298501956 2.2736313230333011E-4 46-47 1.0334687831969551E-5 0.0 1.0334687831969551E-5 0.7254020736137747 2.325304762193149E-4 48-49 1.0334687831969551E-5 0.0 1.0334687831969551E-5 0.8796886282572483 2.3769782013529966E-4 50-51 1.0334687831969551E-5 5.1673439159847756E-6 1.0334687831969551E-5 1.0430283694415268 2.3769782013529966E-4 52-53 1.0334687831969551E-5 1.0334687831969551E-5 1.0334687831969551E-5 1.2017175011014194 2.480325079672692E-4 54-55 1.0334687831969551E-5 1.0334687831969551E-5 1.0334687831969551E-5 1.3725550583077921 2.5836719579923876E-4 56-57 2.5836719579923877E-5 1.0334687831969551E-5 1.0334687831969551E-5 1.5709138892106995 2.635345397152235E-4 58-59 4.1338751327878204E-5 1.0334687831969551E-5 1.0334687831969551E-5 1.7959568840957525 2.945386032111322E-4 60-61 4.1338751327878204E-5 1.0334687831969551E-5 1.0334687831969551E-5 2.0243379831505317 2.9970594712711696E-4 62-63 4.1338751327878204E-5 1.0334687831969551E-5 1.0334687831969551E-5 2.258651193020861 2.9970594712711696E-4 64-65 4.1338751327878204E-5 1.0334687831969551E-5 1.0334687831969551E-5 2.4969225883308352 3.2037532279105606E-4 66-67 4.650609524386298E-5 1.0334687831969551E-5 1.0334687831969551E-5 2.7578166153049906 3.6171407411893426E-4 68-69 6.200812699181731E-5 1.0334687831969551E-5 1.0334687831969551E-5 3.0491462979442963 3.8238344978287336E-4 70-71 6.200812699181731E-5 1.0334687831969551E-5 1.0334687831969551E-5 3.3642974360362916 3.9271813761484293E-4 72-73 6.200812699181731E-5 1.0334687831969551E-5 1.0334687831969551E-5 3.6765806982549156 4.960650159345385E-4 74-75 6.200812699181731E-5 1.0334687831969551E-5 1.0334687831969551E-5 3.977537142609701 5.115670476824928E-4 76-77 6.200812699181731E-5 1.0334687831969551E-5 1.0334687831969551E-5 4.311357894270149 5.322364233464319E-4 78-79 6.200812699181731E-5 1.5502031747954328E-5 1.0334687831969551E-5 4.674596334844299 5.374037672624167E-4 80-81 6.200812699181731E-5 2.0669375663939102E-5 1.0334687831969551E-5 5.058101098256941 5.425711111784015E-4 82-83 7.751015873977163E-5 2.0669375663939102E-5 1.0334687831969551E-5 5.450007962876974 5.580731429263557E-4 84-85 8.267750265575641E-5 3.1004063495908655E-5 1.0334687831969551E-5 5.829849079453183 5.632404868423405E-4 86-87 8.267750265575641E-5 3.1004063495908655E-5 1.0334687831969551E-5 6.226427222973267 5.684078307583252E-4 88 8.267750265575641E-5 3.1004063495908655E-5 1.0334687831969551E-5 6.544286049277239 5.890772064222644E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17305 0.0 48.29505 1 GTATCAA 31030 0.0 41.158485 1 CAACGCA 35805 0.0 34.20121 5 ATCAACG 36655 0.0 33.42076 3 GTACTAG 7580 0.0 33.275677 1 TATCAAC 39180 0.0 32.428444 2 AACGCAG 40245 0.0 31.128986 6 GTGGTAT 5635 0.0 30.314053 1 GCATCGT 7780 0.0 29.725046 8 TCAACGC 41940 0.0 29.410904 4 TACCTGG 5870 0.0 28.538996 2 TAGTACT 10735 0.0 28.331865 4 CTGTGCG 10675 0.0 28.31277 9 ACGCAGA 43550 0.0 28.043547 7 TAGGCAT 9815 0.0 27.92259 5 CGCAGAG 44310 0.0 27.464226 8 GTACATG 42370 0.0 27.321714 1 TCTAACG 3810 0.0 26.925163 2 TACATGG 42650 0.0 26.811062 2 CTAACGC 3975 0.0 26.726774 3 >>END_MODULE