FastQCFastQC Report
Mon 6 Jun 2016
ERR1211253_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211253_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9676151
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297960.30793235864136476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244490.25267278280382355No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152020.1571079244216011No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA179300.048.8324781
GTATCAA313850.039.4491921
GTACTAG78450.033.6862531
CAACGCA364150.032.490715
ATCAACG369500.032.147353
TATCAAC402350.030.6415352
GTGGTAT58000.029.9162921
AACGCAG410550.029.3680046
TACCTGG57650.028.9431172
TCAACGC419400.028.5802574
TAGTACT108850.026.4719814
GGGCACC97300.026.28390594
ACGCAGA448400.026.2812337
CTGTGCG110000.026.1845369
GTACATG434950.026.1736831
CGCAGAG456350.025.771518
TACATGG439300.025.6569022
GCATCGT96650.025.3287038
ACATGGG438350.024.9212323
TCTAACG36800.024.65052
Produced by FastQC (version 0.11.5)