FastQCFastQC Report
Mon 6 Jun 2016
ERR1211252_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211252_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3886930
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143930.3702922357747631No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115080.29606913425248205No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74380.1913592475295413No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66550.050.0688861
GTATCAA114800.039.39711
TACCTGG28750.033.230382
CAACGCA138600.031.4413665
ATCAACG139500.031.306323
GTACTAG24000.031.1794621
GTGGTAT27200.030.7988031
TATCAAC145950.030.6979942
GCATCGT22750.028.7196528
AACGCAG154400.028.7110656
TCAACGC156900.028.0139034
GTACCTG35200.027.542751
GTACATG146400.026.9714091
ACCTGGG34750.026.650293
CTAACGC11350.026.5078583
TAACGCC11650.026.2284364
TCTAACG12000.025.8845022
TACATGG149100.025.8829232
CTGTGCG28900.025.8609859
ACGCAGA169300.025.739627
Produced by FastQC (version 0.11.5)