##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211252_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3886930 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.47895768640032 31.0 31.0 34.0 30.0 34.0 2 31.48964761392667 31.0 31.0 34.0 28.0 34.0 3 31.557799600198614 33.0 31.0 34.0 28.0 34.0 4 35.14898647518736 37.0 35.0 37.0 32.0 37.0 5 35.20564069844325 37.0 35.0 37.0 32.0 37.0 6 35.273216651701986 37.0 35.0 37.0 32.0 37.0 7 35.192978777595684 37.0 35.0 37.0 32.0 37.0 8 35.19572001553926 37.0 35.0 37.0 32.0 37.0 9 36.774570933873264 39.0 37.0 39.0 32.0 39.0 10-11 36.72383140421876 39.0 37.0 39.0 32.0 39.0 12-13 36.62086016470582 39.0 37.0 39.0 32.0 39.0 14-15 37.82485406220333 40.0 37.5 41.0 32.0 41.0 16-17 37.79840143763845 40.0 37.0 41.0 32.0 41.0 18-19 37.811949276163965 40.0 37.5 41.0 32.0 41.0 20-21 37.78141541010515 40.0 37.5 41.0 32.0 41.0 22-23 37.67491812304312 40.0 37.0 41.0 32.0 41.0 24-25 37.58393706601353 40.0 37.0 41.0 32.0 41.0 26-27 37.43297268023865 40.0 37.0 41.0 31.0 41.0 28-29 37.311480268489525 40.0 36.5 41.0 31.0 41.0 30-31 37.15063481976779 40.0 36.0 41.0 31.0 41.0 32-33 36.95515651169432 39.0 36.0 41.0 30.0 41.0 34-35 36.79828206322213 39.0 35.5 41.0 30.0 41.0 36-37 36.68511254383279 39.0 35.0 41.0 30.0 41.0 38-39 36.543537187446134 39.0 35.0 41.0 30.0 41.0 40-41 36.3789449514141 39.0 35.0 41.0 29.0 41.0 42-43 36.16947732529272 39.0 35.0 40.0 28.5 41.0 44-45 35.95462279999897 38.5 35.0 40.0 27.5 41.0 46-47 35.716834236788415 38.0 35.0 40.0 27.0 41.0 48-49 35.51569953665232 38.0 34.0 40.0 26.0 41.0 50-51 34.83911917631653 37.5 33.5 39.5 25.0 40.5 52-53 34.94451996820113 38.0 33.5 40.0 26.0 40.5 54-55 35.425739722608846 38.0 34.0 40.0 26.0 41.0 56-57 35.392019022724874 38.0 34.0 40.0 26.0 41.0 58-59 35.189948622691944 38.0 34.0 40.0 26.0 41.0 60-61 34.9661335552737 37.5 34.0 40.0 26.0 41.0 62-63 34.67326990709892 37.0 34.0 40.0 26.0 41.0 64-65 34.427034317571966 36.5 33.0 40.0 26.0 41.0 66-67 34.25867702788577 36.0 33.0 39.5 25.5 41.0 68-69 33.9653788465447 36.0 33.0 39.0 25.0 41.0 70-71 33.65518686984329 35.0 33.0 39.0 24.5 41.0 72-73 33.318291299303056 35.0 32.5 38.0 24.0 40.0 74-75 32.98907788408847 35.0 32.0 37.0 24.0 39.5 76-77 32.65762877129251 35.0 32.0 37.0 23.0 39.0 78-79 32.322354274453104 35.0 32.0 36.0 23.0 39.0 80-81 32.02519327592728 35.0 31.0 36.0 22.0 38.5 82-83 31.733957647809454 35.0 31.0 35.5 21.0 37.0 84-85 31.479684738341057 35.0 31.0 35.0 20.0 37.0 86-87 31.25807423853787 34.0 31.0 35.0 20.0 36.5 88-89 31.045706766008138 34.0 31.0 35.0 20.0 36.0 90-91 30.82379937379886 34.0 30.0 35.0 19.0 36.0 92-93 30.63180170468725 34.0 30.0 35.0 19.0 35.5 94-95 30.461158935200785 34.0 30.0 35.0 18.0 35.0 96-97 30.28327343173147 34.0 29.5 35.0 18.0 35.0 98-99 30.05911310468673 34.0 29.0 35.0 17.5 35.0 100 28.93661655856936 33.0 27.0 35.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4669696354020765 1101 2 -0.44054090480719665 1101 3 -0.5180239563906603 1101 4 -0.39394873160211574 1101 5 -0.2725697738142898 1101 6 -0.2973181384934378 1101 7 -0.37675672881946554 1101 8 -0.32555151892790946 1101 9 -0.5168690206153528 1101 10-11 -0.5557808466015857 1101 12-13 -0.5751135817596165 1101 14-15 -0.8737610345554216 1101 16-17 -0.7546016766309052 1101 18-19 -0.7822747311669147 1101 20-21 -0.8045365876267638 1101 22-23 -0.855185615773209 1101 24-25 -0.9898783549876597 1101 26-27 -0.8564035326294146 1101 28-29 -0.7900504497313321 1101 30-31 -0.9175229672483738 1101 32-33 -1.0686022300657854 1101 34-35 -0.9050800896310918 1101 36-37 -0.9490825355253136 1101 38-39 -0.9930876947270377 1101 40-41 -1.0084692494150431 1101 42-43 -1.0204346319314084 1101 44-45 -1.0683847016864405 1101 46-47 -1.2114237470142584 1101 48-49 -1.0371591559682614 1101 50-51 -1.151178391206713 1101 52-53 -1.0869310417030675 1101 54-55 -0.9863806038242657 1101 56-57 -0.8491045100793855 1101 58-59 -0.7345740985690483 1101 60-61 -0.816566391635547 1101 62-63 -0.7667656554319819 1101 64-65 -0.7383991393838372 1101 66-67 -0.8011272694993892 1101 68-69 -1.0117721957701846 1101 70-71 -1.0851196991879348 1101 72-73 -1.009074647965349 1101 74-75 -0.9953186970971046 1101 76-77 -1.0180814792825643 1101 78-79 -1.119829807929328 1101 80-81 -1.0823349617905684 1101 82-83 -1.0348139104683192 1101 84-85 -1.0810851076475423 1101 86-87 -1.1016752155030431 1101 88-89 -1.1950943652763044 1101 90-91 -1.2938715295003185 1101 92-93 -1.4414707135430014 1101 94-95 -1.4409097595038922 1101 96-97 -1.4737641403038992 1101 98-99 -1.562922370970238 1101 100 -1.7491628171796627 1102 1 -0.4138699587974415 1102 2 -0.40659123596683955 1102 3 -0.4253087462936911 1102 4 -0.29924983060215027 1102 5 -0.2672277496416484 1102 6 -0.3194823150345414 1102 7 -0.407452203963409 1102 8 -0.4663409993529015 1102 9 -0.6124291726826456 1102 10-11 -0.6297497577476108 1102 12-13 -0.6280472649235307 1102 14-15 -0.8056658052984531 1102 16-17 -0.7798467888526375 1102 18-19 -0.8058606648331974 1102 20-21 -0.8258681094914166 1102 22-23 -0.7211225019848513 1102 24-25 -0.7805328276941523 1102 26-27 -0.7626396622250553 1102 28-29 -0.8821562333660822 1102 30-31 -1.0060606687553033 1102 32-33 -0.8818580135703655 1102 34-35 -0.9149195576391307 1102 36-37 -0.8917075095219147 1102 38-39 -0.9060486372640781 1102 40-41 -0.928791474051323 1102 42-43 -1.01766703878927 1102 44-45 -1.1813799598925243 1102 46-47 -1.0615070628720176 1102 48-49 -1.0201569009265796 1102 50-51 -1.0209506791119836 1102 52-53 -0.7973899386113601 1102 54-55 -0.7057128222482945 1102 56-57 -0.7225127388021377 1102 58-59 -0.7315552687064795 1102 60-61 -0.6899385074417737 1102 62-63 -0.7677946635940884 1102 64-65 -0.8562516268541245 1102 66-67 -0.624047457350386 1102 68-69 -0.7160221485504437 1102 70-71 -0.8037406611234914 1102 72-73 -0.9508496246872156 1102 74-75 -0.9370327017785129 1102 76-77 -0.9004283687049011 1102 78-79 -0.8796350316543027 1102 80-81 -1.0507204129211019 1102 82-83 -1.0025594491164078 1102 84-85 -0.9515170441640599 1102 86-87 -1.1644417739681323 1102 88-89 -1.1096035021381283 1102 90-91 -1.2125197524886744 1102 92-93 -1.153919012234109 1102 94-95 -1.1941941662740696 1102 96-97 -1.138674571452654 1102 98-99 -1.2518754382040065 1102 100 -1.323470895798657 1103 1 -0.43068503286873394 1103 2 -0.39414710598023817 1103 3 -0.5346611437602249 1103 4 -0.3412869866793784 1103 5 -0.3073096740129273 1103 6 -0.3706936755005543 1103 7 -0.4222078541312655 1103 8 -0.3857765804511075 1103 9 -0.6862397393831898 1103 10-11 -0.6279951367218999 1103 12-13 -0.645016551587112 1103 14-15 -0.7584489132035586 1103 16-17 -0.8343152136920864 1103 18-19 -0.7034775450762325 1103 20-21 -0.7324150750605298 1103 22-23 -0.7786304492854157 1103 24-25 -0.8834984062551072 1103 26-27 -0.8010696518652622 1103 28-29 -0.8268990410316022 1103 30-31 -0.9049603340297239 1103 32-33 -0.9260409184146496 1103 34-35 -0.9627329907646995 1103 36-37 -0.9718396936044584 1103 38-39 -0.9806465469569545 1103 40-41 -0.9126228800053724 1103 42-43 -1.051581232770232 1103 44-45 -1.1368065785081427 1103 46-47 -1.004946735467577 1103 48-49 -1.2822133423283617 1103 50-51 -1.2509006611591786 1103 52-53 -1.0994652599649157 1103 54-55 -0.8350345090646201 1103 56-57 -0.8217262179513654 1103 58-59 -0.7293559993379901 1103 60-61 -0.7875099012785967 1103 62-63 -0.7526281054473927 1103 64-65 -0.7187570389468831 1103 66-67 -0.8489441178195207 1103 68-69 -0.7460371937409604 1103 70-71 -0.8667201232947619 1103 72-73 -0.7799175628025878 1103 74-75 -0.8978933097937087 1103 76-77 -0.8701174339535562 1103 78-79 -0.856782467439551 1103 80-81 -0.8693232216008653 1103 82-83 -0.8188951820991548 1103 84-85 -0.961969190275056 1103 86-87 -0.9987872096864727 1103 88-89 -0.9668049615777754 1103 90-91 -1.0985087834922815 1103 92-93 -1.2347884782140959 1103 94-95 -1.1752510886693592 1103 96-97 -1.1486322116263636 1103 98-99 -1.124116512249735 1103 100 -1.317502880686881 1104 1 -0.4116706320413819 1104 2 -0.4070035343866678 1104 3 -0.42712181453012477 1104 4 -0.3143128680568523 1104 5 -0.2607475115147935 1104 6 -0.2908868879624933 1104 7 -0.34356965805843487 1104 8 -0.36191384661667314 1104 9 -0.4038963474595576 1104 10-11 -0.5046408563131308 1104 12-13 -0.4489488498533021 1104 14-15 -0.6046821400314073 1104 16-17 -0.6521424969948626 1104 18-19 -0.7966947807030351 1104 20-21 -0.6603268782027669 1104 22-23 -0.6828630571362382 1104 24-25 -0.6646533831091475 1104 26-27 -0.7650037074239009 1104 28-29 -0.751943069457127 1104 30-31 -0.7926325030348025 1104 32-33 -0.9165105303910153 1104 34-35 -0.8896162892458719 1104 36-37 -0.9219759198117217 1104 38-39 -0.8722924475637299 1104 40-41 -0.8399563545201474 1104 42-43 -0.9478471545285885 1104 44-45 -1.0292465446245984 1104 46-47 -1.0972155217567874 1104 48-49 -0.944019608285366 1104 50-51 -0.9539324952246417 1104 52-53 -0.8361373101084695 1104 54-55 -0.6525224683274686 1104 56-57 -0.8785821975033343 1104 58-59 -0.7909822189101519 1104 60-61 -0.7077527728706556 1104 62-63 -0.8630740556492142 1104 64-65 -0.862675525567262 1104 66-67 -0.8795052275440867 1104 68-69 -0.8788296323534652 1104 70-71 -0.839546126673504 1104 72-73 -0.7612962700568886 1104 74-75 -0.9380204900297571 1104 76-77 -0.8926589545930099 1104 78-79 -0.8913777407233567 1104 80-81 -1.0765693136676084 1104 82-83 -1.080135406058627 1104 84-85 -1.05744731799129 1104 86-87 -1.0858316552486933 1104 88-89 -0.9681912522618177 1104 90-91 -1.0728925216970389 1104 92-93 -1.120891819184255 1104 94-95 -0.9631639522743107 1104 96-97 -1.2569791424838748 1104 98-99 -1.1689402447645172 1104 100 -1.052019804678615 1105 1 -0.6038668610092621 1105 2 -0.495529653936412 1105 3 -0.5704639055100102 1105 4 -0.45973996064930134 1105 5 -0.2949037489781219 1105 6 -0.3279912796338138 1105 7 -0.3699076507743868 1105 8 -0.45698232144892614 1105 9 -0.6085903935709283 1105 10-11 -0.5910505489826718 1105 12-13 -0.6619404252070851 1105 14-15 -0.7721187978617579 1105 16-17 -0.7815749149705837 1105 18-19 -0.732678796687054 1105 20-21 -0.7413311888527474 1105 22-23 -0.7480966424485658 1105 24-25 -0.6842839493932544 1105 26-27 -0.795611800118607 1105 28-29 -0.7043331990126163 1105 30-31 -0.8812772441204899 1105 32-33 -0.9427342279532951 1105 34-35 -0.9074683977672748 1105 36-37 -0.8442518328750381 1105 38-39 -0.9139136216652162 1105 40-41 -0.9034217080642364 1105 42-43 -0.9469334225912363 1105 44-45 -0.9808722024313568 1105 46-47 -1.0987487523057666 1105 48-49 -1.1351240943504806 1105 50-51 -1.1216251175629353 1105 52-53 -0.8608064991871061 1105 54-55 -0.7188056339807147 1105 56-57 -0.6505716647290285 1105 58-59 -0.5942395521281156 1105 60-61 -0.6738909653868035 1105 62-63 -0.8692635747914892 1105 64-65 -0.7092468282803068 1105 66-67 -0.8133837112650895 1105 68-69 -0.874462582080902 1105 70-71 -0.7979408421942153 1105 72-73 -0.7004340613993989 1105 74-75 -0.6505572052830075 1105 76-77 -0.6471871429400977 1105 78-79 -0.7288684390014559 1105 80-81 -0.7428113000277037 1105 82-83 -0.9292671797966996 1105 84-85 -0.8071582763242979 1105 86-87 -0.8516538645354679 1105 88-89 -0.9204242161483229 1105 90-91 -0.8140062111571851 1105 92-93 -0.8624711060391341 1105 94-95 -0.9631756335064665 1105 96-97 -0.9530581815550399 1105 98-99 -1.0017896394643024 1105 100 -1.0245319382143734 1106 1 -0.6468863083550858 1106 2 -0.6123074491961802 1106 3 -0.6150144214077748 1106 4 -0.44017705676002095 1106 5 -0.36369135605794867 1106 6 -0.41599868020288966 1106 7 -0.5444664506083257 1106 8 -0.44786900592195167 1106 9 -0.5685665843630616 1106 10-11 -0.5791603898059847 1106 12-13 -0.6970144972451067 1106 14-15 -0.8286188063921358 1106 16-17 -0.7727839336558802 1106 18-19 -0.7718913109280265 1106 20-21 -0.6844886765263638 1106 22-23 -0.818122062948369 1106 24-25 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46-47 -0.9998799184369318 1107 48-49 -1.0239995432259263 1107 50-51 -1.1144468903847056 1107 52-53 -0.9788286122092131 1107 54-55 -0.9306956358707126 1107 56-57 -0.9427952519526173 1107 58-59 -0.8913289492175167 1107 60-61 -0.8110805325763693 1107 62-63 -0.7934206339485428 1107 64-65 -0.7832574122908866 1107 66-67 -0.8237106815920612 1107 68-69 -0.8888700664883871 1107 70-71 -0.9069230761764473 1107 72-73 -0.7509141218794682 1107 74-75 -0.7078412725670731 1107 76-77 -0.7855126012655553 1107 78-79 -0.832454717587737 1107 80-81 -0.8526912849076886 1107 82-83 -0.8670776126071331 1107 84-85 -0.9180191721851934 1107 86-87 -0.919605102486706 1107 88-89 -0.939265644989753 1107 90-91 -0.9562968934478668 1107 92-93 -1.0303372939053226 1107 94-95 -1.0206392509700848 1107 96-97 -1.0430600715569298 1107 98-99 -1.0106168932915587 1107 100 -1.1481399518223867 1108 1 -0.5344748676983855 1108 2 -0.5102691519943185 1108 3 -0.4935865032178093 1108 4 -0.3032563178764818 1108 5 -0.33501889324917755 1108 6 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1.2164478378938384 2310 32-33 1.2612407460957868 2310 34-35 1.2975999540470937 2310 36-37 1.1969168300362654 2310 38-39 1.301596940083833 2310 40-41 1.3345255546438537 2310 42-43 1.3709400939663112 2310 44-45 1.3960448755501744 2310 46-47 1.4133128577661722 2310 48-49 1.559536973304084 2310 50-51 1.4130996770675424 2310 52-53 1.4167077404470518 2310 54-55 1.3469255851706734 2310 56-57 1.2168174294465075 2310 58-59 1.2385480240953441 2310 60-61 1.1906326762533865 2310 62-63 1.18725989724512 2310 64-65 1.2475790720713107 2310 66-67 1.2266895951724592 2310 68-69 1.1624452933358427 2310 70-71 1.2046557258458748 2310 72-73 1.2076000289207869 2310 74-75 1.192489691678098 2310 76-77 1.1949622683941286 2310 78-79 1.1405672050760032 2310 80-81 1.129192079480731 2310 82-83 1.2201997467194055 2310 84-85 1.2285413306037007 2310 86-87 1.2566220993389727 2310 88-89 1.2580859734150884 2310 90-91 1.3093766579386816 2310 92-93 1.309611358991905 2310 94-95 1.3837818195502471 2310 96-97 1.3996880302390053 2310 98-99 1.5651347408937148 2310 100 1.6359540388671583 2311 1 0.7179313407889403 2311 2 0.7235113051045516 2311 3 0.7412843462382419 2311 4 0.5657745248651835 2311 5 0.5185827645083947 2311 6 0.5688318971893622 2311 7 0.6175249166581835 2311 8 0.5297509452843414 2311 9 0.716444571464038 2311 10-11 0.7727256747935556 2311 12-13 0.8039636706970086 2311 14-15 1.0454736097306423 2311 16-17 1.0946988113031395 2311 18-19 0.9752832412749868 2311 20-21 1.0276098700883125 2311 22-23 1.0889263345744098 2311 24-25 1.0681436230343166 2311 26-27 1.1167558322490265 2311 28-29 1.1302322355528176 2311 30-31 1.1367946839514431 2311 32-33 1.1477552825362167 2311 34-35 1.1425715622726855 2311 36-37 1.1564674478442427 2311 38-39 1.1535788858272937 2311 40-41 1.1971077624652295 2311 42-43 1.270648994991177 2311 44-45 1.326880045320891 2311 46-47 1.2628896093325963 2311 48-49 1.3622285530021756 2311 50-51 1.3409822750444604 2311 52-53 1.1254971971165872 2311 54-55 1.0938217386466604 2311 56-57 1.046291472134115 2311 58-59 0.9712549533663903 2311 60-61 0.9871879556921215 2311 62-63 0.9478193422914245 2311 64-65 0.9221717531382794 2311 66-67 1.0332896154082363 2311 68-69 1.0418011541828278 2311 70-71 1.0065147022613345 2311 72-73 0.9278176868523431 2311 74-75 0.9455267108009124 2311 76-77 0.9775880818351297 2311 78-79 1.059263429130418 2311 80-81 1.0662796090632014 2311 82-83 1.0956978551683356 2311 84-85 1.0936009644349447 2311 86-87 1.100923558041952 2311 88-89 1.2238914681673556 2311 90-91 1.1871126699616958 2311 92-93 1.344052370484345 2311 94-95 1.3110001906693576 2311 96-97 1.2924463139494549 2311 98-99 1.4125060648314012 2311 100 1.4955280018455674 2312 1 0.6116640441626195 2312 2 0.5407606844467487 2312 3 0.6074395395563066 2312 4 0.33461009384961926 2312 5 0.41454685295934723 2312 6 0.5265389274620631 2312 7 0.3877394044471032 2312 8 0.46798712241719187 2312 9 0.7635892569011915 2312 10-11 0.7236333266863184 2312 12-13 0.827738857856609 2312 14-15 1.0081153025387124 2312 16-17 1.0141165476823417 2312 18-19 0.9516164527220354 2312 20-21 1.0118607024433501 2312 22-23 0.9870882252231681 2312 24-25 1.0401128884449165 2312 26-27 1.0774124304669215 2312 28-29 1.1072000575731877 2312 30-31 1.1205126166317214 2312 32-33 1.0920476845300229 2312 34-35 1.0727172965734084 2312 36-37 1.0905998259739889 2312 38-39 1.139963914983774 2312 40-41 1.1568025335435408 2312 42-43 1.175848471521654 2312 44-45 1.2187029509616565 2312 46-47 1.2877442118229254 2312 48-49 1.2603780304949055 2312 50-51 1.2982838570001647 2312 52-53 1.1988352776000255 2312 54-55 1.0693658967652482 2312 56-57 0.9838798557294979 2312 58-59 0.958324101381244 2312 60-61 0.9438871627897498 2312 62-63 0.9812023603241826 2312 64-65 0.9996353315913424 2312 66-67 1.0257917323805756 2312 68-69 1.0053595922421508 2312 70-71 0.9232690949789557 2312 72-73 0.9766678821911583 2312 74-75 1.0170550686077249 2312 76-77 1.004093826528262 2312 78-79 1.03478944864505 2312 80-81 1.0548900091709754 2312 82-83 1.0815229932526655 2312 84-85 1.0461823235311947 2312 86-87 1.1077959719677786 2312 88-89 1.0281374411086048 2312 90-91 1.0873962480298651 2312 92-93 1.1416415720008644 2312 94-95 1.126000238782364 2312 96-97 1.1373561020597691 2312 98-99 0.8264472899810293 2312 100 0.9699563230864996 2313 1 0.622417442922103 2313 2 0.5879413848287456 2313 3 0.5957855313276603 2313 4 0.47000637144997626 2313 5 0.31176095886306143 2313 6 0.48484999673686957 2313 7 0.5056826412864979 2313 8 0.5949610293179504 2313 9 0.6814003999492755 2313 10-11 0.7284033304712167 2313 12-13 0.7213672475123545 2313 14-15 0.946346266485655 2313 16-17 0.9309819399392083 2313 18-19 0.840129179650333 2313 20-21 0.857833959724168 2313 22-23 0.9418330357164137 2313 24-25 0.9021707723555821 2313 26-27 0.9503994435396947 2313 28-29 0.9731755667314488 2313 30-31 0.953952624050558 2313 32-33 0.9638811174855775 2313 34-35 1.1099686749639162 2313 36-37 1.0258576654697507 2313 38-39 1.0744881726189348 2313 40-41 1.0382950065936498 2313 42-43 1.1111833625843701 2313 44-45 1.1136846133019276 2313 46-47 1.2065052094187863 2313 48-49 1.09923997295477 2313 50-51 1.1993554704764833 2313 52-53 1.0660423382499715 2313 54-55 0.8524944384958317 2313 56-57 0.8143759366891601 2313 58-59 0.8057949232004731 2313 60-61 0.8227104723911083 2313 62-63 0.8552617038514398 2313 64-65 0.8917925245237583 2313 66-67 0.8333413284011186 2313 68-69 0.7933116045168092 2313 70-71 0.7945129784949074 2313 72-73 0.7710799148204543 2313 74-75 0.80134657720901 2313 76-77 0.5930459572930076 2313 78-79 0.8093647004081532 2313 80-81 0.8704942179836905 2313 82-83 0.9343123779005005 2313 84-85 0.9687313242711859 2313 86-87 0.9651144651740431 2313 88-89 1.030786533885955 2313 90-91 1.0794979317587234 2313 92-93 1.163826126140453 2313 94-95 1.1758849137893748 2313 96-97 1.2545816296141794 2313 98-99 1.2908835696794903 2313 100 1.4710979221213698 2314 1 0.5113175174538043 2314 2 0.5412054142740672 2314 3 0.510960680832607 2314 4 0.3536697989899693 2314 5 0.37453184275255325 2314 6 0.4292726586137263 2314 7 0.46728291821946755 2314 8 0.4599208846346059 2314 9 0.6428169968528579 2314 10-11 0.5702036066977172 2314 12-13 0.6553934431103912 2314 14-15 0.8031358928027643 2314 16-17 0.7574334537099148 2314 18-19 0.8164530004775301 2314 20-21 0.8574639504341945 2314 22-23 0.8654212604135978 2314 24-25 0.8271666218933831 2314 26-27 0.9106830525696878 2314 28-29 0.8664089004180155 2314 30-31 0.8710281553980295 2314 32-33 0.9075955515568097 2314 34-35 0.9632056822510648 2314 36-37 0.9493107793760984 2314 38-39 0.9941493536108723 2314 40-41 1.0010079606277245 2314 42-43 1.131746695105278 2314 44-45 1.1274403047336108 2314 46-47 1.1804606175593406 2314 48-49 1.1401970872002138 2314 50-51 1.1792354426418896 2314 52-53 1.1288335329119406 2314 54-55 0.9976378678726192 2314 56-57 0.9723222173451802 2314 58-59 0.9742303932270744 2314 60-61 0.9945908260458083 2314 62-63 0.9275689369590552 2314 64-65 0.9920138236360927 2314 66-67 0.9922351507472129 2314 68-69 0.9734398979527228 2314 70-71 1.001548630082148 2314 72-73 1.093546984876724 2314 74-75 1.0832274253868732 2314 76-77 1.0973544902900585 2314 78-79 1.0570121831086752 2314 80-81 1.0848126061207921 2314 82-83 1.1192119367152067 2314 84-85 1.141630748858173 2314 86-87 1.1839974786568597 2314 88-89 1.2612961399982616 2314 90-91 1.3780080041357188 2314 92-93 1.4213093211839194 2314 94-95 1.3435582883094206 2314 96-97 1.3763747352549274 2314 98-99 1.4196609318223317 2314 100 1.5192450498249102 2315 1 0.4676991236817223 2315 2 0.4293847644155093 2315 3 0.531086191352582 2315 4 0.22992621776688082 2315 5 0.28221470689033623 2315 6 0.3942807388882059 2315 7 0.4321035474868111 2315 8 0.4447811093770042 2315 9 0.5846923625243292 2315 10-11 0.47761945078108425 2315 12-13 0.5440225602871465 2315 14-15 0.7639607180615045 2315 16-17 0.7393642683123502 2315 18-19 0.6764440947795904 2315 20-21 0.7966689824286775 2315 22-23 0.6331555723973992 2315 24-25 0.7872542463636947 2315 26-27 0.8337222990592466 2315 28-29 0.8231840472546352 2315 30-31 0.8553566920447011 2315 32-33 0.8743782035341354 2315 34-35 0.9152754715390969 2315 36-37 0.9589813697331593 2315 38-39 0.962806870680275 2315 40-41 0.9700143744343421 2315 42-43 1.140263822731015 2315 44-45 1.0592321020479432 2315 46-47 1.0840896761576602 2315 48-49 1.0180317067740745 2315 50-51 0.9687667920477239 2315 52-53 0.9147186850880757 2315 54-55 0.8890678776427947 2315 56-57 0.8118120115609031 2315 58-59 0.7636879426743732 2315 60-61 0.7891137241803889 2315 62-63 0.7844467647000997 2315 64-65 0.8535457971685716 2315 66-67 0.8338062102998265 2315 68-69 0.8607118591872833 2315 70-71 0.7848970251749066 2315 72-73 0.6554414524448617 2315 74-75 0.8096164977031961 2315 76-77 0.8509992230587713 2315 78-79 0.7967555864663183 2315 80-81 0.5137487488760968 2315 82-83 0.5246622522577304 2315 84-85 0.8413938345715586 2315 86-87 0.9630215867024852 2315 88-89 0.9295265847399783 2315 90-91 0.9778542889845632 2315 92-93 1.126710847986569 2315 94-95 1.129660580110997 2315 96-97 0.8927760770917175 2315 98-99 0.33430287022195415 2315 100 0.823008696826264 2316 1 0.26269393846641265 2316 2 0.21085135971889457 2316 3 0.22280825654976866 2316 4 0.15460334007405407 2316 5 0.09804588092765698 2316 6 0.13774112210030864 2316 7 0.12883018061177154 2316 8 0.174682771841276 2316 9 0.26581886870592086 2316 10-11 0.22233047669844552 2316 12-13 -0.01573495827505411 2316 14-15 0.01039216065520776 2316 16-17 0.18471770900045215 2316 18-19 0.2328163358674118 2316 20-21 0.3259032662879875 2316 22-23 0.2542072016007637 2316 24-25 0.40006145584639086 2316 26-27 0.3439519336540684 2316 28-29 0.37642836530441315 2316 30-31 0.2558545707542308 2316 32-33 0.4686187655708025 2316 34-35 0.4125555937184586 2316 36-37 0.387478049452433 2316 38-39 0.5319979044698684 2316 40-41 0.6931708996719621 2316 42-43 0.5453101120340662 2316 44-45 0.4844753133374837 2316 46-47 0.3224058755255612 2316 48-49 0.200054278437527 2316 50-51 0.259527105106919 2316 52-53 0.3695601075509529 2316 54-55 0.18276965659286049 2316 56-57 -0.009500766581602704 2316 58-59 0.07195298706098185 2316 60-61 0.11977849928437934 2316 62-63 0.3153237596281713 2316 64-65 0.22541133814484482 2316 66-67 0.19434897307549903 2316 68-69 0.16673624059254877 2316 70-71 -0.09079261209240741 2316 72-73 -0.12456044662209109 2316 74-75 0.09009579605787366 2316 76-77 0.2578496259659815 2316 78-79 0.27819783522303254 2316 80-81 0.4820885249967972 2316 82-83 0.5438733297367584 2316 84-85 0.6138544770444341 2316 86-87 0.533618038969454 2316 88-89 0.6401300232494229 2316 90-91 0.3884183118692306 2316 92-93 0.525056027188775 2316 94-95 0.6265736885386417 2316 96-97 0.5533243954552205 2316 98-99 0.41788151483281766 2316 100 0.39515581169693803 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 10.0 1 217.0 2 87.0 3 82.0 4 72.0 5 62.0 6 80.0 7 424.0 8 1310.0 9 2287.0 10 3170.0 11 4370.0 12 5926.0 13 7216.0 14 8650.0 15 10483.0 16 12748.0 17 16097.0 18 19874.0 19 24119.0 20 29200.0 21 35526.0 22 39457.0 23 40144.0 24 39501.0 25 40482.0 26 44124.0 27 50178.0 28 59073.0 29 70128.0 30 85327.0 31 106417.0 32 135454.0 33 177576.0 34 252793.0 35 354230.0 36 532653.0 37 767574.0 38 710641.0 39 198918.0 40 250.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.43020346382751 19.513092320682055 12.780045923515724 23.276658291974712 2 15.865260389737692 19.801073694032496 41.19960151880381 23.134064397426 3 19.6351348778182 25.654472760171863 28.986109517175645 25.724282844834285 4 12.704311338817744 16.09698408789675 38.42328165675875 32.77542291652675 5 13.184015142996172 37.50228053295894 34.63504910897744 14.678655215067446 6 30.546416214104095 36.89260560208025 18.259734964532033 14.301243219283618 7 27.486443664464446 32.03084396152613 22.454388545877883 18.028323828131537 8 25.909715680066718 35.50205144836888 20.111812944973824 18.47641992659058 9 26.244939002936928 16.082253572457162 21.277434136581224 36.395373288024686 10-11 25.530510839793834 26.105650220337335 27.631822774762792 20.73201616510604 12-13 25.662149216884433 23.8817304262288 28.113879782095847 22.34224057479092 14-15 22.978490011298835 25.2661376378871 26.139423674445304 25.61594867636876 16-17 21.97776381411726 27.89724512760024 27.13833132884472 22.98665972943778 18-19 21.409454672334828 27.408318878595384 29.51380874561179 21.668417703457994 20-21 22.555954107319746 26.719248223210947 29.2192781371091 21.50551953236021 22-23 22.62784955211467 26.548324253049454 28.93859430952293 21.885231885312944 24-25 22.155900267884025 26.462168136469028 29.52655808914542 21.855373506501525 26-27 22.137039224735368 26.71471435462096 28.90233501367721 22.245911406966457 28-29 21.718025932962128 27.104991417178148 29.175302881877673 22.001679767982047 30-31 22.428518017903272 26.80528334065387 29.158287937022315 21.607910704420547 32-33 22.438080935523473 26.93403714436215 28.660558027483212 21.967323892631164 34-35 22.494370318409285 26.879706358484434 29.028519345492743 21.597403977613542 36-37 22.284697250652712 26.745766820394607 28.776293164896504 22.193242764056173 38-39 22.383070702781986 26.967824942986816 28.927328695081588 21.72177565914961 40-41 22.3264828326611 26.351825327672735 29.257030675427593 22.064661164238576 42-43 22.169741581562374 26.540225023524926 28.79545284636526 22.49458054854744 44-45 22.17778984565415 27.108634050800042 28.770522181586077 21.94305392195973 46-47 22.16283283146989 26.913775321496754 28.2831946055621 22.64019724147126 48-49 22.50539646066304 26.863101180645476 28.31591821418396 22.31558414450753 50-51 22.361960735166317 26.960739026457148 28.208117669432497 22.46918256894404 52-53 22.11261550110651 27.336161608830377 27.969299994736712 22.581922895326397 54-55 22.008873995949052 27.19089643315807 28.08965875779499 22.710570813097895 56-57 22.530896885044246 26.92321600158746 27.828152850193327 22.717734263174968 58-59 22.052575367855 27.164021563032904 28.26690771442481 22.516495354687287 60-61 22.231287373914903 27.52819946959213 27.846490512513167 22.3940226439798 62-63 22.326517740181238 27.172519371950326 27.72595955214745 22.775003335720985 64-65 21.94025767957655 27.118862148091516 27.90091531396512 23.039964858366815 66-67 22.09819380662007 26.911891471154526 27.8505736462557 23.139341075969703 68-69 21.891282689560263 26.985013073121717 27.978680992924122 23.145023244393897 70-71 21.87645819933664 26.770191259130115 28.074305655825192 23.279044885708057 72-73 22.159452640438484 26.900833226624904 28.07819937081706 22.86151476211956 74-75 22.29648658577148 27.06373117629991 27.76160174271419 22.87818049521442 76-77 22.119537415302084 26.90478322582372 27.690969809817602 23.2847095490566 78-79 22.001527205683214 26.90313694737702 27.755322865048154 23.34001298189161 80-81 21.821188406005952 27.185042146035578 27.942592560187666 23.051176887770804 82-83 22.330475327830044 26.656738271198304 28.511190995738723 22.50159540523293 84-85 22.377502992752426 26.921443545528756 27.81041162648134 22.89064183523748 86-87 21.687988265733633 27.267605677291463 28.047209811765168 22.997196245209732 88-89 22.16194392823489 26.56466388796969 28.07297501900297 23.200417164792444 90-91 22.1978020564147 26.582714305289386 28.049712664197628 23.169770974098284 92-93 22.30517392710663 26.713674062328803 28.011498130434457 22.96965388013011 94-95 21.670801326230492 26.629382034695116 28.35635348630866 23.343463152765732 96-97 21.803283139130105 26.551731192420807 28.166345064013658 23.478640604435427 98-99 21.539747052455873 26.78774015161721 27.95809830118059 23.714414494746332 100 22.018714812577798 26.454379137984237 27.61504133384151 23.911864715596458 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1450.0 1 1022.5 2 1401.0 3 2096.0 4 1444.5 5 1000.0 6 1491.0 7 2643.5 8 4027.0 9 4507.0 10 3722.5 11 2961.5 12 2968.5 13 3350.5 14 3841.0 15 4271.0 16 4704.0 17 5186.0 18 5688.5 19 6146.0 20 6561.5 21 7144.5 22 8036.0 23 9384.5 24 11037.0 25 12967.5 26 15830.5 27 19289.5 28 23345.0 29 29526.0 30 37868.5 31 46655.0 32 57290.0 33 68810.0 34 79112.0 35 89092.0 36 98363.0 37 111492.0 38 118745.5 39 117293.5 40 115412.0 41 112919.0 42 113597.5 43 118851.0 44 126540.5 45 138519.5 46 150758.0 47 159192.5 48 164635.0 49 164972.0 50 162014.0 51 162356.5 52 159010.0 53 150608.0 54 141736.5 55 126453.5 56 112135.5 57 95398.0 58 79224.5 59 71059.5 60 58378.5 61 43559.0 62 33625.0 63 26775.0 64 19795.0 65 12598.0 66 8654.5 67 6348.5 68 4830.5 69 3763.5 70 2964.5 71 2326.5 72 1775.0 73 1362.5 74 1069.0 75 774.0 76 551.5 77 385.5 78 245.0 79 158.0 80 115.5 81 92.5 82 66.5 83 51.5 84 49.0 85 38.0 86 28.0 87 22.0 88 19.5 89 15.5 90 11.5 91 10.0 92 11.0 93 9.5 94 5.0 95 3.5 96 1.5 97 1.0 98 0.5 99 2.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05685721121810786 2 0.1335501282503158 3 0.017288708569488002 4 0.016542618467530933 5 0.019990069283470503 6 0.051866125708464 7 0.05698584744258322 8 0.04810994795378358 9 0.04062331968931779 10-11 0.03920832122008886 12-13 0.03992868407715086 14-15 0.017726071732704216 16-17 0.016774163671586576 18-19 0.017005708875642216 20-21 0.019977205661022967 22-23 0.02435083729318511 24-25 0.023669065303465717 26-27 0.01856220719179404 28-29 0.017468799283753503 30-31 0.015462074181937931 32-33 0.02184243091591565 34-35 0.02137934050780436 36-37 0.02171379469144029 38-39 0.019745660456967323 40-41 0.02284579346682343 42-43 0.029805013210940253 44-45 0.03859086734260715 46-47 0.04484258785210951 48-49 0.038925321526243076 50-51 0.02729660683367079 52-53 0.03919545759764133 54-55 0.036532687751001436 56-57 0.03770327739372718 58-59 0.04295163535232176 60-61 0.022228339589341718 62-63 0.024788200456401323 64-65 0.02420933744626222 66-67 0.031297193414854395 68-69 0.0379991407100205 70-71 0.04046895621994736 72-73 0.04457245178071126 74-75 0.02669201657863661 76-77 0.019707069589624714 78-79 0.019231115559065894 80-81 0.023617610813675572 82-83 0.019809978569205 84-85 0.011770214539495179 86-87 0.013751212396415679 88-89 0.017906162446969718 90-91 0.020517477803819465 92-93 0.018858070508087358 94-95 0.022601384640320254 96-97 0.023759110660598468 98-99 0.02638328963989575 100 0.02909751397632579 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3886930.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.93561373371314 #Duplication Level Percentage of deduplicated Percentage of total 1 82.54154232649122 60.20218048109898 2 11.828568757513489 17.25447843841342 3 2.961074668243871 6.479033947191533 4 1.0622907745109025 3.099153184104566 5 0.5212492727767624 1.9008817809112404 6 0.2985116933518151 1.3063280136782767 7 0.18202422599842488 0.9293234046319456 8 0.12044166468741391 0.7027589386469295 9 0.08915966912111094 0.5852623668878716 >10 0.37341414373372395 4.512444580150729 >50 0.015180740477215102 0.7623984935234522 >100 0.005759049051004963 0.739130848042928 >500 4.6980842875455096E-4 0.2316651329692055 >1k 1.9575350890558681E-4 0.34297175835224075 >5k 3.915070178111736E-5 0.21239063680159576 >10k+ 7.830140356223472E-5 0.7395979945950701 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14393 0.3702922357747631 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11508 0.29606913425248205 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7438 0.1913592475295413 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 2.5727244895071432E-5 10-11 0.0 0.0 0.0 0.0 2.5727244895071432E-5 12-13 0.0 0.0 0.0 1.2863622447535716E-5 2.5727244895071432E-5 14-15 0.0 0.0 0.0 2.5727244895071432E-5 3.859086734260715E-5 16-17 0.0 0.0 0.0 1.0290897958028573E-4 5.1454489790142864E-5 18-19 2.5727244895071432E-5 0.0 0.0 1.0290897958028573E-4 5.1454489790142864E-5 20-21 2.5727244895071432E-5 0.0 0.0 2.186815816081072E-4 5.1454489790142864E-5 22-23 2.5727244895071432E-5 0.0 0.0 5.531357652440358E-4 7.71817346852143E-5 24-25 2.5727244895071432E-5 0.0 0.0 0.0011448623978306785 1.0290897958028573E-4 26-27 2.5727244895071432E-5 0.0 0.0 0.002019588724263107 1.0290897958028573E-4 28-29 2.5727244895071432E-5 0.0 0.0 0.005055403621881537 1.0290897958028573E-4 30-31 2.5727244895071432E-5 0.0 0.0 0.017584571885781324 1.0290897958028573E-4 32-33 2.5727244895071432E-5 0.0 0.0 0.04830290229049661 1.0290897958028573E-4 34-35 2.5727244895071432E-5 0.0 0.0 0.09233508192841136 1.0290897958028573E-4 36-37 2.5727244895071432E-5 0.0 0.0 0.1635095049306265 1.0290897958028573E-4 38-39 2.5727244895071432E-5 0.0 0.0 0.27085643425531203 1.0290897958028573E-4 40-41 2.5727244895071432E-5 0.0 0.0 0.3853298104159324 1.0290897958028573E-4 42-43 2.5727244895071432E-5 0.0 0.0 0.4964457811177459 1.1577260202782144E-4 44-45 2.5727244895071432E-5 0.0 0.0 0.6242330065115658 1.4149984692289288E-4 46-47 2.5727244895071432E-5 0.0 0.0 0.7775931133310865 1.8009071426550004E-4 48-49 5.1454489790142864E-5 0.0 0.0 0.9477788383119841 2.572724489507143E-4 50-51 5.1454489790142864E-5 0.0 0.0 1.121193332527213 2.572724489507143E-4 52-53 5.1454489790142864E-5 0.0 0.0 1.2877772432228005 2.9586331629332145E-4 54-55 5.1454489790142864E-5 0.0 0.0 1.4693344104473196 3.60181428531E-4 56-57 5.1454489790142864E-5 0.0 0.0 1.680001955270612 3.60181428531E-4 58-59 5.1454489790142864E-5 0.0 0.0 1.9193939690192516 3.859086734260715E-4 60-61 5.1454489790142864E-5 0.0 0.0 2.160676935267679 3.859086734260715E-4 62-63 5.1454489790142864E-5 0.0 0.0 2.4063978512605066 3.859086734260715E-4 64-65 5.1454489790142864E-5 0.0 0.0 2.6567496713344463 3.859086734260715E-4 66-67 5.1454489790142864E-5 0.0 0.0 2.939170502170093 3.859086734260715E-4 68-69 5.1454489790142864E-5 0.0 0.0 3.242726264687041 3.859086734260715E-4 70-71 5.1454489790142864E-5 0.0 0.0 3.5678157311811636 3.859086734260715E-4 72-73 5.1454489790142864E-5 0.0 0.0 3.887258067420818 4.6309040811128575E-4 74-75 5.1454489790142864E-5 0.0 0.0 4.202738407946631 4.6309040811128575E-4 76-77 5.1454489790142864E-5 0.0 0.0 4.5552531174989 4.888176530063572E-4 78-79 5.1454489790142864E-5 0.0 0.0 4.943798833526716 4.888176530063572E-4 80-81 5.1454489790142864E-5 0.0 0.0 5.344616445369482 4.888176530063572E-4 82-83 5.1454489790142864E-5 0.0 0.0 5.747968190834412 5.145448979014286E-4 84-85 5.1454489790142864E-5 0.0 0.0 6.1445408072694905 5.145448979014286E-4 86-87 5.1454489790142864E-5 0.0 0.0 6.559315449467832 5.402721427965001E-4 88 5.1454489790142864E-5 0.0 0.0 6.900047080858158 5.402721427965001E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6655 0.0 50.068886 1 GTATCAA 11480 0.0 39.3971 1 TACCTGG 2875 0.0 33.23038 2 CAACGCA 13860 0.0 31.441366 5 ATCAACG 13950 0.0 31.30632 3 GTACTAG 2400 0.0 31.179462 1 GTGGTAT 2720 0.0 30.798803 1 TATCAAC 14595 0.0 30.697994 2 GCATCGT 2275 0.0 28.719652 8 AACGCAG 15440 0.0 28.711065 6 TCAACGC 15690 0.0 28.013903 4 GTACCTG 3520 0.0 27.54275 1 GTACATG 14640 0.0 26.971409 1 ACCTGGG 3475 0.0 26.65029 3 CTAACGC 1135 0.0 26.507858 3 TAACGCC 1165 0.0 26.228436 4 TCTAACG 1200 0.0 25.884502 2 TACATGG 14910 0.0 25.882923 2 CTGTGCG 2890 0.0 25.860985 9 ACGCAGA 16930 0.0 25.73962 7 >>END_MODULE