FastQCFastQC Report
Mon 6 Jun 2016
ERR1211252_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211252_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3886930
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134110.345028081287803No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111940.2879907793554296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73230.1884006143666081No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68450.048.4901431
GTATCAA118300.039.1447641
TACCTGG26600.038.6921232
GTACTAG24050.032.4500351
CAACGCA140950.031.9678215
ATCAACG142300.031.609083
TATCAAC149800.030.5567552
GTACCTG35150.030.0939641
GTGGTAT27950.029.4359761
AACGCAG155700.029.3316966
TCAACGC156550.029.0320824
ACCTGGG35800.028.4892833
CTGTGCG29350.026.7345879
GTACATG144000.026.5103681
TACATGG143650.026.139742
ACGCAGA172550.026.0589267
TAGGCAT32400.025.6677475
CTAACGC12100.025.6367743
CGCAGAG176700.025.4199858
ACATGGG141150.025.3734133
Produced by FastQC (version 0.11.5)