FastQCFastQC Report
Mon 6 Jun 2016
ERR1211251_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211251_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5644813
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185600.3287974287190736No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143160.25361336150550956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91160.16149339225232084No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92800.046.904861
GTATCAA159200.040.8792761
TACCTGG46150.034.8718262
CAACGCA188400.033.2353865
ATCAACG189350.032.9693343
TATCAAC202400.031.7120382
GTACTAG30600.031.3713251
AACGCAG207300.030.6819956
GTACCTG53850.030.4974731
TCAACGC209000.030.094244
ACCTGGG53750.029.0360163
ACGCAGA229950.027.169437
GTACATG208200.026.8961661
TACATGG207000.026.5062622
CGCAGAG236450.026.4211398
ACATGGG205050.025.9974483
GCATCGT32950.025.9646118
TAGGCAT42800.025.1518025
CTAGGCA45850.024.606254
CATGGGG157450.023.9743274
Produced by FastQC (version 0.11.5)