FastQCFastQC Report
Mon 6 Jun 2016
ERR1211251_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211251_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5644813
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168510.2985218465164391No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136940.24259439595253202No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96120.17028022008877883No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99900.046.4607351
GTATCAA162250.037.5045551
TACCTGG47000.035.4032862
GTACCTG57350.029.6831231
ATCAACG200750.029.364493
CAACGCA201900.029.2321415
ACCTGGG56100.028.8254473
GTACTAG30000.028.5288721
TATCAAC215600.028.0369242
AACGCAG221000.027.513726
TCAACGC217550.027.2697264
GTACATG205600.025.8915331
TACATGG207350.025.4120522
GTGGTAT41450.024.8458611
ACATGGG207550.024.3028643
ACGCAGA247900.023.9215977
CGCAGAG254350.023.29658
TGGGGGG121550.023.0408576
CATGGGG160550.022.8676324
TAGGCAT40300.022.7371945
Produced by FastQC (version 0.11.5)