FastQCFastQC Report
Mon 6 Jun 2016
ERR1211250_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211250_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3711307
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239380.6450018820862838No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174900.4712625498240916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119230.3212614855090134No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111060.2992476774354695No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64230.17306571512407892No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48330.1302236651400706No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG43350.059.4889072
ACCTGGG48650.051.598173
GGTATCA91850.048.470111
GTACCTG58900.044.6640971
TATCACG3200.042.6473962
GTATCAA164050.041.5675281
CCTGGGG38100.039.3587764
TAACGCA5400.038.303184
ATAACGC5750.035.154143
ATCAACG197950.034.0066153
CAACGCA202950.033.145645
TATCAAC205100.032.948322
TCAACGC209300.032.229874
GTACATG163700.031.4219821
AACGCAG216500.031.3751536
CTGGGGG33750.031.0603965
GTACACG4000.030.5897161
TACATGG167800.030.1762182
GTGGTAT44250.029.4597151
CATGGGG99500.029.1027164
Produced by FastQC (version 0.11.5)