FastQCFastQC Report
Mon 6 Jun 2016
ERR1211250_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211250_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3711307
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219330.5909777876095941No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173690.4680022428756231No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118010.3179742338750203No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88940.23964603305520132No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55140.14857299598227794No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48580.13089728227818395No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG45300.060.3854062
ACCTGGG50250.053.6016853
GGTATCA90500.051.178561
GTACCTG61050.045.365951
CCTGGGG35050.042.7821434
GTATCAA161300.039.821421
TAACGCA5300.037.2506034
CTGGGGG28350.035.1386155
GTACACG5250.033.139261
ATCAACG194600.032.4409143
CAACGCA200300.031.8112415
TATCAAC201650.031.4194722
TCAACGC205700.030.8960024
GTACCCG3400.030.4259531
GTACATG160950.030.2085551
AACGCAG214650.030.0786066
ATAACGC6600.029.913363
TACATGG164100.028.9567472
ACATGGG159500.028.705043
TGGTATC40750.028.0275962
Produced by FastQC (version 0.11.5)