FastQCFastQC Report
Mon 6 Jun 2016
ERR1211249_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211249_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5502706
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238180.43284158739354783No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183780.33398113582662786No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118240.21487609914104078No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG52500.051.8018952
GGTATCA104400.050.7935331
ACCTGGG55150.048.7534033
GTATCAA197850.041.7612461
GTACCTG65200.041.7124061
CCTGGGG46200.035.712444
ATCAACG231750.034.1364863
CAACGCA233100.034.0194445
TATCAAC249450.032.6694222
GCATCGT31850.031.4352348
AACGCAG257150.031.368456
GTACTAG37650.030.9935781
TCAACGC259750.030.7824484
GTACATG264050.030.346841
TACATGG268100.029.2560632
CTGGGGG43550.029.1427175
ACATGGG264450.028.9377843
GTACACG6250.028.6080721
TATCACG2650.028.4086912
ACGCAGA279000.028.3560817
Produced by FastQC (version 0.11.5)