FastQCFastQC Report
Mon 6 Jun 2016
ERR1211249_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211249_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5502706
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214920.390571475197839No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176710.3211329116983535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117530.21358582486507546No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA74480.13535158883647427No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG52300.056.1653142
ACCTGGG56750.050.857563
GGTATCA107900.049.632461
GTACCTG67250.044.466031
CCTGGGG43700.039.3688434
GTATCAA189450.039.014091
GTACACG6500.037.6143531
CTGGGGG38750.032.984365
ATCAACG223450.031.8912733
CAACGCA227500.031.395915
CTGTGCG45350.030.7744339
TATCAAC245050.029.8815122
GTACATG255350.029.4978391
AACGCAG252650.029.1819676
TCAACGC250750.028.6441154
TACATGG261000.028.199482
ACATGGG254050.028.0315063
GCATCGT39400.027.3117898
TAGTACT55050.027.0679234
GGGCACC47400.026.9763494
Produced by FastQC (version 0.11.5)