##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211249_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5502706 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16654533242372 33.0 31.0 34.0 30.0 34.0 2 32.295169140419276 34.0 31.0 34.0 30.0 34.0 3 32.37639790314075 34.0 31.0 34.0 30.0 34.0 4 35.9916621022457 37.0 35.0 37.0 35.0 37.0 5 35.88941568021261 37.0 35.0 37.0 35.0 37.0 6 35.91905636972064 37.0 35.0 37.0 35.0 37.0 7 35.864995149659094 37.0 35.0 37.0 35.0 37.0 8 35.85183162611268 37.0 35.0 37.0 35.0 37.0 9 37.45944213628713 39.0 37.0 39.0 35.0 39.0 10-11 37.43712220860064 39.0 37.0 39.0 34.5 39.0 12-13 37.33668335179092 39.0 37.0 39.0 34.0 39.0 14-15 38.553150032002435 40.0 38.0 41.0 34.0 41.0 16-17 38.512360736699364 40.0 38.0 41.0 34.0 41.0 18-19 38.56025662646705 40.0 38.0 41.0 34.0 41.0 20-21 38.528675709732624 40.0 38.0 41.0 34.0 41.0 22-23 38.39461503122282 40.0 38.0 41.0 34.0 41.0 24-25 38.33265705999921 40.0 38.0 41.0 34.0 41.0 26-27 38.19448713051361 40.0 38.0 41.0 34.0 41.0 28-29 38.16712068571354 40.0 37.5 41.0 33.5 41.0 30-31 38.15920521648803 40.0 37.5 41.0 34.0 41.0 32-33 38.00730940377335 40.0 37.0 41.0 33.0 41.0 34-35 37.85546156745427 40.0 37.0 41.0 33.0 41.0 36-37 37.703976370898246 40.0 37.0 41.0 33.0 41.0 38-39 37.60861474336444 40.0 37.0 41.0 33.0 41.0 40-41 37.509433449651866 40.0 36.0 41.0 32.5 41.0 42-43 37.36047455560955 40.0 36.0 41.0 32.0 41.0 44-45 37.20579574849174 40.0 36.0 41.0 31.5 41.0 46-47 36.95602754353949 39.5 36.0 41.0 31.0 41.0 48-49 36.70855875273002 39.0 35.5 41.0 30.0 41.0 50-51 36.51355732979374 39.0 35.0 41.0 30.0 41.0 52-53 36.324529785890796 39.0 35.0 40.5 29.5 41.0 54-55 36.100906535802565 39.0 35.0 40.0 29.0 41.0 56-57 35.85800177221898 38.0 35.0 40.0 28.5 41.0 58-59 35.600684735837234 38.0 34.5 40.0 28.0 41.0 60-61 35.353329525509814 38.0 34.0 40.0 28.0 41.0 62-63 35.058562187403794 37.0 34.0 40.0 27.0 41.0 64-65 34.70300802914057 36.5 34.0 39.5 26.0 41.0 66-67 34.410891750349734 36.0 33.5 39.0 26.0 41.0 68-69 34.08849009923481 36.0 33.0 39.0 26.0 40.0 70-71 33.77629806135381 35.0 33.0 38.5 26.0 40.0 72-73 33.43234001598486 35.0 33.0 38.0 25.5 40.0 74-75 33.08880966927907 35.0 32.0 37.0 25.0 39.5 76-77 32.068262233163104 34.0 31.0 36.0 24.0 39.0 78-79 32.54862843844465 35.0 32.0 36.0 25.0 39.0 80-81 32.46499758482463 35.0 32.0 36.0 25.0 37.5 82-83 32.196220641262684 35.0 32.0 35.5 25.0 37.0 84-85 31.929359573271768 34.5 32.0 35.0 24.0 37.0 86-87 31.688452463206282 34.0 31.0 35.0 24.0 36.0 88-89 31.463848786397094 34.0 31.0 35.0 23.5 36.0 90-91 31.239466727824457 34.0 31.0 35.0 23.0 36.0 92-93 31.05286880672891 34.0 31.0 35.0 22.0 35.0 94-95 30.86472864441604 34.0 31.0 35.0 20.0 35.0 96-97 30.69915874117207 34.0 30.0 35.0 20.0 35.0 98-99 30.534509112425777 34.0 30.0 35.0 20.0 35.0 100 28.753632849001928 32.0 26.0 34.0 16.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3316657065354143 1101 2 -0.29214516047657924 1101 3 -0.38082819314079686 1101 4 -0.1879719487455418 1101 5 -0.24125787609068539 1101 6 -0.32352200802358055 1101 7 -0.25942815793048624 1101 8 -0.29628742315854595 1101 9 -0.4476696234164521 1101 10-11 -0.46830473683159113 1101 12-13 -0.4449341432062681 1101 14-15 -0.6444266309725606 1101 16-17 -0.5917129388722557 1101 18-19 -0.6081504477429647 1101 20-21 -0.6987876862254581 1101 22-23 -0.7153297779703962 1101 24-25 -0.6835114942991325 1101 26-27 -0.8185651344433325 1101 28-29 -0.7414333512654139 1101 30-31 -0.6535723142679402 1101 32-33 -0.623920500144358 1101 34-35 -0.6320573926116211 1101 36-37 -0.5923608178557984 1101 38-39 -0.705627015849565 1101 40-41 -0.7189338178350084 1101 42-43 -0.6175724314092079 1101 44-45 -0.6851143156783621 1101 46-47 -0.7107486242454684 1101 48-49 -0.6408441944255046 1101 50-51 -0.6784523791558783 1101 52-53 -0.6905009547153895 1101 54-55 -0.6698387586148868 1101 56-57 -0.6332735355375192 1101 58-59 -0.5896210592203204 1101 60-61 -0.7175919831241302 1101 62-63 -0.8724919082122682 1101 64-65 -0.6925055224239856 1101 66-67 -0.749255747411226 1101 68-69 -0.8163551052716187 1101 70-71 -0.8904926417579091 1101 72-73 -0.8744993670212438 1101 74-75 -0.8402745705043273 1101 76-77 -0.8105780986262694 1101 78-79 -0.7091066811415168 1101 80-81 -0.5487045499813021 1101 82-83 -0.5544768225190708 1101 84-85 -0.5703141792213948 1101 86-87 -0.733592858061126 1101 88-89 -0.6349413488106208 1101 90-91 -0.7593005570834599 1101 92-93 -0.8175084607279075 1101 94-95 -0.7686547862064295 1101 96-97 -0.8111787379799864 1101 98-99 -0.8795062043475355 1101 100 -0.7777856978431785 1102 1 -0.39635734024550473 1102 2 -0.337898372571658 1102 3 -0.34915508433777376 1102 4 -0.21800234021532816 1102 5 -0.26116039266794644 1102 6 -0.32409677414072036 1102 7 -0.36779091957355803 1102 8 -0.4466527024723135 1102 9 -0.4777148108456899 1102 10-11 -0.5520265149108141 1102 12-13 -0.5287100240932645 1102 14-15 -0.6580250932658913 1102 16-17 -0.7039145000004439 1102 18-19 -0.6503418171378712 1102 20-21 -0.694070457944413 1102 22-23 -0.7574798690773221 1102 24-25 -0.6797695798946606 1102 26-27 -0.7373816154024198 1102 28-29 -0.8436359533950366 1102 30-31 -0.7828628630521095 1102 32-33 -0.6556575283051771 1102 34-35 -0.5825006637039607 1102 36-37 -0.8370014591614563 1102 38-39 -0.7612551081898502 1102 40-41 -0.722272318779126 1102 42-43 -0.6120634567045684 1102 44-45 -0.6789913289806151 1102 46-47 -0.7000449060574425 1102 48-49 -0.6120908690437759 1102 50-51 -0.6734192530757284 1102 52-53 -0.7376245002857829 1102 54-55 -0.7102281305471436 1102 56-57 -0.8370477368392244 1102 58-59 -0.920905700920919 1102 60-61 -0.8853346087102167 1102 62-63 -0.86579391150795 1102 64-65 -0.7803186068567598 1102 66-67 -0.9424094984450662 1102 68-69 -0.906210255673976 1102 70-71 -0.9762615465126245 1102 72-73 -0.9936040155499342 1102 74-75 -1.1939306622467605 1102 76-77 -0.8615235746620762 1102 78-79 -0.7817273231877877 1102 80-81 -0.7086752117074191 1102 82-83 -0.6761257257015032 1102 84-85 -0.7581228899035217 1102 86-87 -0.8051881075988447 1102 88-89 -0.8519415322561308 1102 90-91 -0.8500155600359456 1102 92-93 -0.9936318185440811 1102 94-95 -0.9485672510340173 1102 96-97 -1.0222146661059277 1102 98-99 -1.0532541806240836 1102 100 -1.1362883402430093 1103 1 -0.3523086763917753 1103 2 -0.33591388392387955 1103 3 -0.3252792957100894 1103 4 -0.23924425301420627 1103 5 -0.2159174350629698 1103 6 -0.36824585993537795 1103 7 -0.411106531387901 1103 8 -0.3183108097658689 1103 9 -0.5045783832788828 1103 10-11 -0.4689603103699298 1103 12-13 -0.5448741189295134 1103 14-15 -0.6739427143227559 1103 16-17 -0.6364120487245728 1103 18-19 -0.6252047265210336 1103 20-21 -0.6779451816735644 1103 22-23 -0.7074298791235378 1103 24-25 -0.756408318090358 1103 26-27 -0.7002011854245183 1103 28-29 -0.7465436688862965 1103 30-31 -0.6841270583504837 1103 32-33 -0.6476260635673796 1103 34-35 -0.7253856345699461 1103 36-37 -0.7068217525108693 1103 38-39 -0.6776686504844065 1103 40-41 -0.6859196766252182 1103 42-43 -0.5759439871445906 1103 44-45 -0.662352409953094 1103 46-47 -0.7145014059569732 1103 48-49 -0.7158412407536403 1103 50-51 -0.8468856585408631 1103 52-53 -0.7716152577702147 1103 54-55 -0.7198006441095259 1103 56-57 -0.6682441202027007 1103 58-59 -0.7739169928638532 1103 60-61 -0.8048463225940807 1103 62-63 -0.7240528024060779 1103 64-65 -0.7887149701278062 1103 66-67 -0.8891680678643965 1103 68-69 -0.8772693274039298 1103 70-71 -0.8977349642341395 1103 72-73 -0.8671239472356902 1103 74-75 -0.7660324705650297 1103 76-77 -0.7332732230446375 1103 78-79 -0.576334194797191 1103 80-81 -0.5120511208896588 1103 82-83 -0.5817295030774332 1103 84-85 -0.6121069164255211 1103 86-87 -0.6950268859793916 1103 88-89 -0.6748270255251647 1103 90-91 -0.7145231951575077 1103 92-93 -0.8166070445838614 1103 94-95 -0.8662294169974523 1103 96-97 -0.8238732757101097 1103 98-99 -0.7915908492999932 1103 100 -0.8779989691359162 1104 1 -0.2983963984919953 1104 2 -0.32079421932016317 1104 3 -0.36022970497341333 1104 4 -0.253726784457605 1104 5 -0.232847241411541 1104 6 -0.2669461993244724 1104 7 -0.2908728320467162 1104 8 -0.3864679668830533 1104 9 -0.4716576406156747 1104 10-11 -0.5352906158201165 1104 12-13 -0.5279310364779235 1104 14-15 -0.6799970165786959 1104 16-17 -0.6035222303975658 1104 18-19 -0.6846545368624177 1104 20-21 -0.6796308474751243 1104 22-23 -0.6773719034796954 1104 24-25 -0.6366185396914759 1104 26-27 -0.6836707402258781 1104 28-29 -0.7850825189561732 1104 30-31 -0.6524955350923491 1104 32-33 -0.6975470457993609 1104 34-35 -0.6691759120704504 1104 36-37 -0.6485775520716501 1104 38-39 -0.6114132302408493 1104 40-41 -0.6972354482363201 1104 42-43 -0.6351079320437734 1104 44-45 -0.7971462410571064 1104 46-47 -0.9180050993089495 1104 48-49 -0.9606335745528085 1104 50-51 -0.868091667724336 1104 52-53 -0.7649624328550644 1104 54-55 -0.8763387155808786 1104 56-57 -0.9066999397275595 1104 58-59 -0.9343537267428772 1104 60-61 -0.8638547873575106 1104 62-63 -0.9625581927035398 1104 64-65 -0.9355211390237983 1104 66-67 -0.7903907071137439 1104 68-69 -0.8343392016303213 1104 70-71 -0.8701677053002186 1104 72-73 -0.9309315136162013 1104 74-75 -1.024846225805593 1104 76-77 -0.9780394438471909 1104 78-79 -0.7604881576640281 1104 80-81 -0.7069578288153906 1104 82-83 -0.6832008546446104 1104 84-85 -0.7501715001747762 1104 86-87 -0.7813935859195027 1104 88-89 -0.7347713856768969 1104 90-91 -0.7858894359241262 1104 92-93 -0.8359420615533182 1104 94-95 -0.8690447094709981 1104 96-97 -0.8723832976705808 1104 98-99 -0.8319687291163227 1104 100 -1.0881897058099028 1105 1 -0.3738622342949043 1105 2 -0.3790896463316926 1105 3 -0.41619534115557144 1105 4 -0.22516392612562441 1105 5 -0.26912815860293193 1105 6 -0.3849231571249305 1105 7 -0.3567079266440345 1105 8 -0.43218555562992833 1105 9 -0.5789853994239849 1105 10-11 -0.5797995569316186 1105 12-13 -0.529930387615984 1105 14-15 -0.7625091883111992 1105 16-17 -0.699239176490174 1105 18-19 -0.6897260141431119 1105 20-21 -0.7981595528116969 1105 22-23 -0.7745566082445237 1105 24-25 -0.8727027171336132 1105 26-27 -0.8176990925403231 1105 28-29 -0.7189862241982325 1105 30-31 -0.6781912600646578 1105 32-33 -0.7540292128597841 1105 34-35 -0.694758068295279 1105 36-37 -0.704846946850104 1105 38-39 -0.6922004025274617 1105 40-41 -0.8372290447735153 1105 42-43 -0.8273887947985585 1105 44-45 -0.8169069046710575 1105 46-47 -0.7028510172784834 1105 48-49 -0.9184368955274138 1105 50-51 -0.900376130934724 1105 52-53 -0.972011510510896 1105 54-55 -0.8528302634039235 1105 56-57 -0.8422021827899044 1105 58-59 -1.0352322808845855 1105 60-61 -0.9451082719662125 1105 62-63 -0.9374483575186972 1105 64-65 -0.8936356996474402 1105 66-67 -0.9101038063535256 1105 68-69 -0.8645762837523492 1105 70-71 -0.9196729351330859 1105 72-73 -0.9366410490893884 1105 74-75 -0.8536516254384523 1105 76-77 -1.0092034602381794 1105 78-79 -0.8956432639620289 1105 80-81 -0.876254848216746 1105 82-83 -0.8549027141597563 1105 84-85 -0.8734984850920533 1105 86-87 -0.7789057777473829 1105 88-89 -0.7351871809965651 1105 90-91 -0.7248890512468016 1105 92-93 -0.6550659443247433 1105 94-95 -0.6670672483707811 1105 96-97 -0.8558041308027917 1105 98-99 -0.8578388859752941 1105 100 -0.967879415617876 1106 1 -0.44219022978983347 1106 2 -0.33237213635106144 1106 3 -0.4707375481372722 1106 4 -0.3159529908782801 1106 5 -0.32731355156781916 1106 6 -0.35805168517809705 1106 7 -0.4139201236209118 1106 8 -0.3715768291833399 1106 9 -0.5789473153177198 1106 10-11 -0.5677904074573092 1106 12-13 -0.6302207208712574 1106 14-15 -0.7227970832547896 1106 16-17 -0.7120266118313694 1106 18-19 -0.7334056973948222 1106 20-21 -0.7471150845861843 1106 22-23 -0.7202764810652837 1106 24-25 -0.7441850171194844 1106 26-27 -0.6775118650361662 1106 28-29 -0.7326960960707609 1106 30-31 -0.7685532719427144 1106 32-33 -0.7550182965770844 1106 34-35 -0.7940118481621781 1106 36-37 -0.7295557330933846 1106 38-39 -0.7967191359213004 1106 40-41 -0.8566662785669621 1106 42-43 -0.7118410745232282 1106 44-45 -0.7843339633511519 1106 46-47 -0.8412975224618364 1106 48-49 -0.9281868718216018 1106 50-51 -0.9846555614534864 1106 52-53 -0.8955181703915756 1106 54-55 -0.9014161780901091 1106 56-57 -1.0214324697172685 1106 58-59 -0.969151944320167 1106 60-61 -1.0007472934678745 1106 62-63 -0.9897275596876369 1106 64-65 -0.9850324458559712 1106 66-67 -0.9592787474760414 1106 68-69 -0.9229101041029608 1106 70-71 -0.9918631195739422 1106 72-73 -1.0619851122842334 1106 74-75 -1.0987771307592027 1106 76-77 -0.9820887570252204 1106 78-79 -0.8897555540204927 1106 80-81 -0.7794870432197527 1106 82-83 -0.7294964933686963 1106 84-85 -0.7798593436099885 1106 86-87 -0.8779744587949274 1106 88-89 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0.6347547091548762 2311 30-31 0.8745654556856977 2311 32-33 0.9095751840932635 2311 34-35 0.9470420011131466 2311 36-37 1.0184454963974332 2311 38-39 0.9934903014806551 2311 40-41 0.9973885579349826 2311 42-43 1.0150432867169243 2311 44-45 0.9605177709577006 2311 46-47 0.5348279764510053 2311 48-49 0.7768339931827271 2311 50-51 0.9181484649775413 2311 52-53 0.9672075744194331 2311 54-55 1.0149489504007647 2311 56-57 1.0317475204710362 2311 58-59 1.0853629821829358 2311 60-61 1.1324476262518246 2311 62-63 1.070551377257047 2311 64-65 1.1322983174579662 2311 66-67 1.0930862053732895 2311 68-69 1.118662675091393 2311 70-71 1.15186254044459 2311 72-73 1.065246171292074 2311 74-75 1.167796163246578 2311 76-77 1.0955458661630963 2311 78-79 0.7695501470618211 2311 80-81 0.7444517487298086 2311 82-83 0.8499049323561323 2311 84-85 0.9014529712850212 2311 86-87 0.9617893336088734 2311 88-89 0.9955833924411444 2311 90-91 1.0368183519416867 2311 92-93 1.0408229616544418 2311 94-95 1.0730122548678018 2311 96-97 1.1038972171158399 2311 98-99 1.0880800178241508 2311 100 1.260340519323222 2312 1 0.21946946431174297 2312 2 0.31996375426707857 2312 3 0.4471573877070014 2312 4 0.17069042035262783 2312 5 0.3442564342837642 2312 6 0.3835317782555805 2312 7 0.28661507665229635 2312 8 0.39106758219393356 2312 9 0.5589894559171 2312 10-11 0.5964806444039397 2312 12-13 0.6393157051910876 2312 14-15 0.8134077881898065 2312 16-17 0.8337128256472894 2312 18-19 0.8084976604136429 2312 20-21 0.8270152454621069 2312 22-23 0.8985972565548508 2312 24-25 0.8829767321608983 2312 26-27 0.930570171359399 2312 28-29 0.9143785672946052 2312 30-31 0.9048694240615802 2312 32-33 0.8164539615582456 2312 34-35 0.9177965262340706 2312 36-37 0.9935975942228055 2312 38-39 0.8061185354452434 2312 40-41 0.8929957756360949 2312 42-43 1.0180361636091817 2312 44-45 0.7661636704489183 2312 46-47 0.8158534935405086 2312 48-49 0.9677250909046506 2312 50-51 0.7721349444742103 2312 52-53 0.999858988460943 2312 54-55 0.8810036272472601 2312 56-57 0.9015375380887605 2312 58-59 1.0025598915582563 2312 60-61 1.0653394814260153 2312 62-63 1.0492085041265824 2312 64-65 1.0828272409274646 2312 66-67 1.0931531632913831 2312 68-69 1.1255767781237083 2312 70-71 1.0595245835045546 2312 72-73 1.0720972839200869 2312 74-75 1.1187019515455248 2312 76-77 1.0825766513272264 2312 78-79 0.9610504785696463 2312 80-81 0.9433932258440123 2312 82-83 0.9561096929024657 2312 84-85 0.9860469780468399 2312 86-87 0.9812699733170867 2312 88-89 0.9892405624145333 2312 90-91 1.056209845043501 2312 92-93 1.073615442853324 2312 94-95 1.098755884510087 2312 96-97 0.7346257192647663 2312 98-99 0.4349440753231235 2312 100 0.6592967595072992 2313 1 0.025060872938311718 2313 2 0.21721473970584526 2313 3 0.2758773425359564 2313 4 0.16667292116363086 2313 5 0.26219876469343006 2313 6 0.18707082703173938 2313 7 0.19817653803962543 2313 8 0.34255423286506925 2313 9 0.5491365984507155 2313 10-11 0.3460409076568638 2313 12-13 0.39712356681957317 2313 14-15 0.4465122186857684 2313 16-17 0.7102741875726295 2313 18-19 0.6504251151354836 2313 20-21 0.5776559145505473 2313 22-23 0.5158954558338351 2313 24-25 0.4409430826404517 2313 26-27 0.6415393748678042 2313 28-29 0.3550356246581643 2313 30-31 0.6519219063267414 2313 32-33 0.7241768596969322 2313 34-35 0.8089298343703675 2313 36-37 0.7630823887802407 2313 38-39 0.789486769734836 2313 40-41 0.8428674122907367 2313 42-43 0.9038528459857389 2313 44-45 0.6677100748215707 2313 46-47 0.7030229545789481 2313 48-49 0.8234417068712006 2313 50-51 0.7140009431643719 2313 52-53 0.7168055704880132 2313 54-55 0.6564419868283551 2313 56-57 0.43609656189229895 2313 58-59 0.47605633234929456 2313 60-61 0.4777089758224804 2313 62-63 0.7661006689702887 2313 64-65 0.7473082941840019 2313 66-67 0.6945958785424295 2313 68-69 0.8264036324011883 2313 70-71 0.7756281295539296 2313 72-73 0.7695357360773443 2313 74-75 0.8781223406408216 2313 76-77 0.8579466307921528 2313 78-79 0.7459717735341087 2313 80-81 0.6531756756998703 2313 82-83 0.6516687916850472 2313 84-85 0.6806220726122341 2313 86-87 0.6928260981862557 2313 88-89 0.6618268112592425 2313 90-91 0.6162819205550392 2313 92-93 0.7737487410434198 2313 94-95 0.8980803272169204 2313 96-97 0.9512883564457972 2313 98-99 0.9828815014917254 2313 100 1.092826122216092 2314 1 -0.47334285091290695 2314 2 -0.001957544611016715 2314 3 0.23238607370311115 2314 4 0.2061485715525322 2314 5 0.3164360462383371 2314 6 0.3984339819618583 2314 7 0.43483527463216376 2314 8 0.4374924130982265 2314 9 0.38775971340704274 2314 10-11 0.5493222983257269 2314 12-13 0.47225446118602576 2314 14-15 0.7429785383376242 2314 16-17 0.7841525909835454 2314 18-19 0.8027654467839298 2314 20-21 0.778815687891651 2314 22-23 0.8106237541996606 2314 24-25 0.7721313596232307 2314 26-27 0.6789325938396971 2314 28-29 0.7718956439747586 2314 30-31 0.7312296762192929 2314 32-33 0.7947064995217019 2314 34-35 0.8043937816659863 2314 36-37 0.7871974630561809 2314 38-39 0.5620398931458723 2314 40-41 0.559922615864906 2314 42-43 0.7067783600734074 2314 44-45 0.7763376782196048 2314 46-47 0.7426740273054122 2314 48-49 0.7259250103865256 2314 50-51 0.7186513131830665 2314 52-53 0.7332202545435607 2314 54-55 0.663488136674907 2314 56-57 0.5914520792500184 2314 58-59 0.7298775951568146 2314 60-61 0.8273818926689174 2314 62-63 0.7729695448119216 2314 64-65 0.80020746830332 2314 66-67 0.5782268242000654 2314 68-69 0.5937179027572128 2314 70-71 0.6951450600893665 2314 72-73 0.8709441281671673 2314 74-75 0.9424199161786646 2314 76-77 0.8209791332946814 2314 78-79 0.6742887301039957 2314 80-81 0.7174345282438139 2314 82-83 0.7486383898991491 2314 84-85 0.7398768172321262 2314 86-87 0.7544116059634511 2314 88-89 0.7179497151254814 2314 90-91 0.9019731563171085 2314 92-93 0.7406987930567581 2314 94-95 0.7579700965324996 2314 96-97 0.696725477710384 2314 98-99 0.8572146234880904 2314 100 0.8139702008245564 2315 1 0.4412273152761301 2315 2 0.47772003959940434 2315 3 0.4340220880125756 2315 4 0.2974069893604465 2315 5 0.37709601531643955 2315 6 0.45012789397028996 2315 7 0.406782383952077 2315 8 0.28915462692510374 2315 9 0.481450481473388 2315 10-11 0.576450119625612 2315 12-13 0.41819612359392266 2315 14-15 0.6627045107925511 2315 16-17 0.7818593245829746 2315 18-19 0.7570311163594923 2315 20-21 0.4939412191596375 2315 22-23 0.8202933168294493 2315 24-25 0.8172656887538636 2315 26-27 0.7825646232349044 2315 28-29 0.7917652285992034 2315 30-31 0.8841944265476016 2315 32-33 0.7844644289188594 2315 34-35 0.8343986981186404 2315 36-37 0.8412363935983436 2315 38-39 0.8569429549314478 2315 40-41 0.8590143659279761 2315 42-43 0.907885532272168 2315 44-45 0.9073918691720309 2315 46-47 0.8975131752065408 2315 48-49 0.7378425563003645 2315 50-51 0.8924608523950113 2315 52-53 0.9485835221732444 2315 54-55 0.69122081103108 2315 56-57 0.7194334872961861 2315 58-59 0.7518463756824971 2315 60-61 0.9045087436162973 2315 62-63 1.0025323039768992 2315 64-65 1.0447346802218433 2315 66-67 1.0120148215865825 2315 68-69 0.9335256435825343 2315 70-71 0.9041670741530012 2315 72-73 1.0068047984866695 2315 74-75 0.31774094112966367 2315 76-77 0.32446431601205106 2315 78-79 0.6994645256313134 2315 80-81 0.7766438203393307 2315 82-83 0.7017655125452436 2315 84-85 0.7250498310822913 2315 86-87 0.778122169220719 2315 88-89 0.8210367298683785 2315 90-91 0.8490355758587107 2315 92-93 0.8245210762314912 2315 94-95 0.9112971117929085 2315 96-97 1.1308191361333648 2315 98-99 1.0694323317742551 2315 100 0.954169964058547 2316 1 0.24564438788326015 2316 2 0.030887909798096302 2316 3 0.21485433657690578 2316 4 -0.033060396511963575 2316 5 0.21496836463808222 2316 6 0.33834606831574376 2316 7 0.4005445896883302 2316 8 0.4286141528649239 2316 9 0.5997564448227664 2316 10-11 0.5966772083146807 2316 12-13 0.32330809332759003 2316 14-15 0.3938211122841153 2316 16-17 0.746650881021317 2316 18-19 0.6753661254969003 2316 20-21 0.6637835178780023 2316 22-23 0.7064062942695912 2316 24-25 0.3075782289948421 2316 26-27 0.5862577084469365 2316 28-29 0.7909673923992102 2316 30-31 0.44568504927283215 2316 32-33 0.6085589887495146 2316 34-35 0.6992548736512774 2316 36-37 0.7043788453964623 2316 38-39 0.7213439055694053 2316 40-41 0.673872999010058 2316 42-43 0.6678823207776858 2316 44-45 0.4417069323488505 2316 46-47 0.6490262472567778 2316 48-49 0.7032666815184072 2316 50-51 0.7989485716534546 2316 52-53 0.7755207661460801 2316 54-55 0.6586639298815058 2316 56-57 0.8021247886164318 2316 58-59 0.7117308397220867 2316 60-61 0.782165037645079 2316 62-63 0.7311587073099162 2316 64-65 0.8430397383923705 2316 66-67 0.8070468798187207 2316 68-69 0.7888995814152011 2316 70-71 0.735060850414591 2316 72-73 0.7409237358734302 2316 74-75 0.7806161094050807 2316 76-77 0.670995835251329 2316 78-79 0.43068344346887955 2316 80-81 0.3580001496634111 2316 82-83 0.28879366583138477 2316 84-85 0.43761580405280753 2316 86-87 0.5424876132732201 2316 88-89 0.46093784741952604 2316 90-91 0.35929818002934155 2316 92-93 0.15524183433990402 2316 94-95 0.20255414055112908 2316 96-97 0.4244904014199342 2316 98-99 0.638790615572951 2316 100 0.34600491726169125 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 6.0 12 32.0 13 142.0 14 553.0 15 1669.0 16 4318.0 17 9352.0 18 15572.0 19 21748.0 20 29380.0 21 40082.0 22 48168.0 23 47048.0 24 44859.0 25 46111.0 26 53048.0 27 64149.0 28 79046.0 29 97417.0 30 120454.0 31 150632.0 32 192219.0 33 253546.0 34 364157.0 35 501919.0 36 782271.0 37 1210378.0 38 1130373.0 39 194029.0 40 27.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.028961708496446 20.645653234738635 13.361427926260511 22.963957130504408 2 15.315301235428533 20.820992435358168 41.644420036251255 22.219286292962046 3 18.599921565862324 27.68841366411362 29.633129591150244 24.07853517887381 4 11.885623265832722 17.155401203343708 39.8457944324493 31.113181098374266 5 12.216589437996506 38.49874952432494 34.74808575998791 14.53657527769065 6 28.450765859560732 38.09589318418974 19.495153838856737 13.958187117392788 7 26.619100528581296 32.70950708512656 23.300610941556567 17.370781444735577 8 25.97636508292466 35.47122088659652 20.656000883928744 17.89641314655008 9 26.768720360024435 15.892524832922003 21.827181074180977 35.511573732872584 10-11 24.962234342165175 26.806888528680865 28.121492337058395 20.109384792095568 12-13 25.206834601012666 24.7648520564246 28.505947800954658 21.522365541608075 14-15 22.548795386262828 25.989117155722262 26.91798885910047 24.54409859891444 16-17 20.981171740491824 28.41509582774667 28.032328212076795 22.571404219684716 18-19 20.44909359140757 27.93426543231639 30.554748881732007 21.061892094544028 20-21 21.899788940205056 27.24055219377521 30.121816429952826 20.73784243606691 22-23 21.816638940913798 27.260169814633016 29.691628082619715 21.23156316183347 24-25 21.206768714096974 27.064125233750165 30.572494807066384 21.156611245086477 26-27 21.138811164093404 27.384207722895603 29.8989473668907 21.578033746120298 28-29 20.6310769153045 27.805033139252465 30.254199355987595 21.309690589455446 30-31 21.670863945908934 27.306318998977297 30.202418959495763 20.820398095618003 32-33 21.65242478527215 27.492126281932904 29.646166160897213 21.209282771897733 34-35 21.65164299133935 27.531567077842055 30.094788259020227 20.722001671798367 36-37 21.457288478399146 27.356219265608583 29.674558799977387 21.511933456014884 38-39 21.34621087643315 27.64189928734418 30.00898760847909 21.00290222774358 40-41 21.41940373030884 26.77784385732089 30.521673509609727 21.281078902760544 42-43 21.316171463022034 26.99310929493281 29.807144762051006 21.88357447999415 44-45 21.15770449261905 27.738928755709896 29.82888800558481 21.274478746086245 46-47 21.246456863871142 27.374136362698632 29.187616788646555 22.19178998478368 48-49 21.605333714250612 27.436148650060783 29.310467028937904 21.648050606750704 50-51 21.56514159366907 27.43875503458747 29.05015926294575 21.945944108797708 52-53 21.149069976792358 27.976949390111027 28.753121326948584 22.120859306148034 54-55 21.228072136411104 27.764581309675474 28.892013754375938 22.115332799537484 56-57 21.810578248393035 27.552100308967713 28.509497692805425 22.12782374983383 58-59 21.037872483269044 27.801233936731435 29.1616103806695 21.999283199330016 60-61 21.481279214312195 28.333602910708706 28.48668045448928 21.698437420489817 62-63 21.581244072145232 27.782804673334883 28.44186308965742 22.194088164862467 64-65 20.99700827707283 27.683903970720692 28.545107133640744 22.773980618565734 66-67 21.19252407859095 27.502995589194366 28.449691785489357 22.854788546725327 68-69 20.875383099551197 27.672550383574606 28.672789228398475 22.779277288475726 70-71 21.03192208541236 27.281087959014982 28.80288906837127 22.884100887201384 72-73 21.271168043658413 27.43250461714345 28.691751176421604 22.60457616277653 74-75 21.47307737966142 27.660770638839466 28.257611882395523 22.60854009910359 76-77 21.12154632958869 27.549303998619866 28.304359104826588 23.024790566964853 78-79 20.96670137327856 27.488627200368747 28.402704298485222 23.141967127867463 80-81 20.69229904610272 27.903178776210275 28.44739971466405 22.95712246302295 82-83 21.49979633793704 27.266091552755746 29.038719607834402 22.19539250147281 84-85 21.541648927383626 27.589314846841233 28.176327155199022 22.692709070576118 86-87 20.559142248515546 27.97132282145906 28.675632944479446 22.793901985545943 88-89 21.181687466884703 27.16675088230927 28.551094192612343 23.100467458193684 90-91 21.25123716707626 27.129475108430245 28.60735737476124 23.011930349732257 92-93 21.292786068950758 27.31340815675194 28.50401734472644 22.889788429570864 94-95 20.427127444659984 27.25679900965859 28.87469167699181 23.441381868689614 96-97 20.58185744331803 27.312281201686723 28.56852859037321 23.537332764622036 98-99 20.173521163421757 27.58207069157896 28.456383817282006 23.78802432771728 100 20.475219295415144 27.23960549821457 28.224151154544707 24.061024051825576 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1542.0 1 1095.5 2 1770.0 3 2457.0 4 1556.5 5 1148.0 6 1585.0 7 2809.0 8 4157.0 9 4750.5 10 4381.5 11 3936.5 12 4239.5 13 4779.5 14 5447.5 15 6254.0 16 7025.0 17 7830.0 18 8604.5 19 9460.5 20 10519.0 21 11656.5 22 13085.0 23 15082.0 24 17429.5 25 20466.0 26 25707.5 27 31924.5 28 37713.0 29 47693.5 30 62961.0 31 77607.5 32 96299.5 33 117758.0 34 136718.0 35 155655.0 36 174019.0 37 196069.0 38 204821.5 39 199746.0 40 190386.5 41 178403.5 42 174375.0 43 177089.5 44 185194.0 45 203903.0 46 217827.5 47 222595.5 48 227414.0 49 224454.5 50 217480.0 51 214762.5 52 207498.0 53 192718.0 54 178469.0 55 155211.5 56 132491.0 57 108259.0 58 84727.5 59 75228.0 60 59627.0 61 40260.0 62 29279.5 63 23593.0 64 16625.5 65 8550.0 66 4937.5 67 3431.0 68 2661.0 69 2033.5 70 1513.0 71 1135.0 72 940.0 73 805.5 74 624.5 75 457.5 76 291.5 77 162.5 78 103.0 79 68.5 80 44.0 81 28.5 82 16.0 83 13.5 84 12.0 85 8.5 86 3.5 87 1.0 88 1.0 89 0.5 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04503238951890215 2 0.0 3 0.0 4 0.03392876159474993 5 0.0 6 0.0 7 9.449896105661469E-4 8 0.0 9 0.011539776975182756 10-11 3.9071685821484917E-4 12-13 0.0 14-15 1.544694555733125E-4 16-17 3.634575425254411E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.54223576545794E-4 26-27 5.270134366618897E-4 28-29 3.08938911146625E-4 30-31 0.0024260790963573195 32-33 0.015592328574341422 34-35 0.026250721008899983 36-37 0.03167532483109219 38-39 0.022579799829393028 40-41 0.0014265708544123565 42-43 6.36050699419522E-5 44-45 0.0115761227294353 46-47 0.019036088789769978 48-49 0.003616402548128139 50-51 0.011657900676503524 52-53 0.011612468483687844 54-55 0.010949158468578914 56-57 4.997541209724815E-4 58-59 0.008704808143484314 60-61 0.004833985315588367 62-63 0.0015083488014805808 64-65 0.036881854127769134 66-67 0.06125168235409996 68-69 0.06032486562066009 70-71 0.05342825875123984 72-73 0.04637718242624629 74-75 0.0362912356211653 76-77 0.03240223991614308 78-79 0.015855835292672368 80-81 0.0012630149602759079 82-83 0.03948057555682604 84-85 0.05281946736750973 86-87 0.05835310845245958 88-89 0.07133762915918096 90-91 0.04982094264167484 92-93 0.040789022709917626 94-95 0.013902251001598124 96-97 0.0037617855651383156 98-99 0.022616145583645574 100 0.0325839686874058 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5502706.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.08391776508883 #Duplication Level Percentage of deduplicated Percentage of total 1 82.62585292496595 58.73369334588603 2 12.435430996592947 17.67918308670499 3 2.9640433555532266 6.320874424149105 4 0.910724487021773 2.589514581684336 5 0.3798636503813734 1.3501098242827998 6 0.1892952156880619 0.8073507327176966 7 0.0983386574622164 0.48932079281214 8 0.06265822748258519 0.35631938317426515 9 0.04640268317415389 0.29686360633479236 >10 0.23066607734549832 3.0956652126335285 >50 0.0273412035923574 1.3710879978275103 >100 0.02637551905447017 3.6977345506639177 >500 0.0022092261716326563 1.086567069213085 >1k 6.750408193823147E-4 0.8527290063866708 >5k 3.0683673608161604E-5 0.1624494996816274 >10k+ 9.205102082448483E-5 1.1105368858471723 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21492 0.390571475197839 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17671 0.3211329116983535 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11753 0.21358582486507546 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 7448 0.13535158883647427 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 1.8172877126272057E-5 0.0 0.0 4 0.0 0.0 1.8172877126272057E-5 1.8172877126272057E-5 0.0 5 0.0 0.0 1.8172877126272057E-5 1.8172877126272057E-5 0.0 6 0.0 0.0 1.8172877126272057E-5 1.8172877126272057E-5 0.0 7 0.0 0.0 1.8172877126272057E-5 1.8172877126272057E-5 0.0 8 0.0 0.0 1.8172877126272057E-5 1.8172877126272057E-5 0.0 9 0.0 0.0 1.8172877126272057E-5 3.6345754252544114E-5 0.0 10-11 0.0 0.0 1.8172877126272057E-5 8.177794706822425E-5 0.0 12-13 0.0 0.0 1.8172877126272057E-5 9.99508241944963E-5 1.8172877126272057E-5 14-15 0.0 0.0 1.8172877126272057E-5 1.0903726275763234E-4 1.8172877126272057E-5 16-17 0.0 0.0 1.8172877126272057E-5 1.7264233269958455E-4 1.8172877126272057E-5 18-19 0.0 0.0 1.8172877126272057E-5 2.9076603402035286E-4 1.8172877126272057E-5 20-21 0.0 0.0 1.8172877126272057E-5 4.7249480528307347E-4 1.8172877126272057E-5 22-23 0.0 0.0 1.8172877126272057E-5 9.631624876924189E-4 1.8172877126272057E-5 24-25 0.0 0.0 1.8172877126272057E-5 0.0020171893610161982 1.8172877126272057E-5 26-27 0.0 0.0 1.8172877126272057E-5 0.003607316109565003 2.7259315689408084E-5 28-29 0.0 0.0 1.8172877126272057E-5 0.007841596479986392 4.543219281568014E-5 30-31 0.0 0.0 1.8172877126272057E-5 0.02443343329627278 5.451863137881617E-5 32-33 0.0 0.0 1.8172877126272057E-5 0.06311440225954285 5.451863137881617E-5 34-35 0.0 0.0 1.8172877126272057E-5 0.11967748231506463 5.451863137881617E-5 36-37 0.0 0.0 1.8172877126272057E-5 0.2092606801090227 5.451863137881617E-5 38-39 0.0 0.0 1.8172877126272057E-5 0.344148860578777 5.451863137881617E-5 40-41 0.0 0.0 1.8172877126272057E-5 0.4927212175246143 5.451863137881617E-5 42-43 0.0 0.0 1.8172877126272057E-5 0.6337881762173011 5.451863137881617E-5 44-45 0.0 0.0 1.8172877126272057E-5 0.7944000642592934 5.451863137881617E-5 46-47 0.0 0.0 1.8172877126272057E-5 0.9808083513820292 5.451863137881617E-5 48-49 0.0 0.0 1.8172877126272057E-5 1.1837721295667984 6.36050699419522E-5 50-51 0.0 0.0 1.8172877126272057E-5 1.3931055011843263 7.269150850508823E-5 52-53 0.0 0.0 2.7259315689408084E-5 1.5977866162575287 7.269150850508823E-5 54-55 0.0 9.086438563136029E-6 3.6345754252544114E-5 1.8257744462451746 7.269150850508823E-5 56-57 0.0 1.8172877126272057E-5 3.6345754252544114E-5 2.0802219853286728 7.269150850508823E-5 58-59 0.0 1.8172877126272057E-5 3.6345754252544114E-5 2.3624285942225516 7.269150850508823E-5 60-61 0.0 1.8172877126272057E-5 3.6345754252544114E-5 2.6509411914792467 7.269150850508823E-5 62-63 0.0 1.8172877126272057E-5 3.6345754252544114E-5 2.942016164410746 7.269150850508823E-5 64-65 0.0 1.8172877126272057E-5 3.6345754252544114E-5 3.235208277527456 7.269150850508823E-5 66-67 0.0 1.8172877126272057E-5 3.6345754252544114E-5 3.5583765514639527 7.269150850508823E-5 68-69 0.0 1.8172877126272057E-5 3.6345754252544114E-5 3.917863320337303 7.269150850508823E-5 70-71 0.0 1.8172877126272057E-5 3.6345754252544114E-5 4.2953594104427895 7.269150850508823E-5 72-73 0.0 1.8172877126272057E-5 3.6345754252544114E-5 4.666077017380177 7.269150850508823E-5 74-75 0.0 1.8172877126272057E-5 3.6345754252544114E-5 5.02739924684328 7.269150850508823E-5 76-77 0.0 1.8172877126272057E-5 3.6345754252544114E-5 5.424531130683704 7.269150850508823E-5 78-79 1.8172877126272057E-5 1.8172877126272057E-5 3.6345754252544114E-5 5.862115839007208 7.269150850508823E-5 80-81 1.8172877126272057E-5 1.8172877126272057E-5 3.6345754252544114E-5 6.31429336766311 7.269150850508823E-5 82-83 1.8172877126272057E-5 1.8172877126272057E-5 3.6345754252544114E-5 6.7715683883529305 7.269150850508823E-5 84-85 1.8172877126272057E-5 1.8172877126272057E-5 3.6345754252544114E-5 7.2282891362904 7.269150850508823E-5 86-87 1.8172877126272057E-5 1.8172877126272057E-5 3.6345754252544114E-5 7.701901573516738 7.269150850508823E-5 88 1.8172877126272057E-5 1.8172877126272057E-5 3.6345754252544114E-5 8.084040833727988 7.269150850508823E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 5230 0.0 56.165314 2 ACCTGGG 5675 0.0 50.85756 3 GGTATCA 10790 0.0 49.63246 1 GTACCTG 6725 0.0 44.46603 1 CCTGGGG 4370 0.0 39.368843 4 GTATCAA 18945 0.0 39.01409 1 GTACACG 650 0.0 37.614353 1 CTGGGGG 3875 0.0 32.98436 5 ATCAACG 22345 0.0 31.891273 3 CAACGCA 22750 0.0 31.39591 5 CTGTGCG 4535 0.0 30.774433 9 TATCAAC 24505 0.0 29.881512 2 GTACATG 25535 0.0 29.497839 1 AACGCAG 25265 0.0 29.181967 6 TCAACGC 25075 0.0 28.644115 4 TACATGG 26100 0.0 28.19948 2 ACATGGG 25405 0.0 28.031506 3 GCATCGT 3940 0.0 27.311789 8 TAGTACT 5505 0.0 27.067923 4 GGGCACC 4740 0.0 26.97634 94 >>END_MODULE