FastQCFastQC Report
Mon 6 Jun 2016
ERR1211248_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211248_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5256016
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191910.3651244592862731No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149100.2836749355405311No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94760.18028864447901224No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85350.049.4087261
TACCTGG44300.041.5393522
GTATCAA155950.041.3460771
GTACCTG54700.034.588991
ATCAACG182700.034.1957933
CAACGCA182300.034.1157765
ACCTGGG53150.033.5214043
TATCAAC191950.033.173892
AACGCAG197800.032.131926
TCAACGC198650.031.5681674
GTACTAG28250.029.655191
GTACATG181300.029.0490151
ACGCAGA218400.028.5417947
TACATGG181300.028.1655792
CGCAGAG223600.027.854098
ACATGGG185500.026.7105583
TAGTACT38950.026.6726974
CTGTGCG37600.026.6271239
GCATCGT26950.026.3368058
GTGGTAT37900.025.8299311
Produced by FastQC (version 0.11.5)