FastQCFastQC Report
Mon 6 Jun 2016
ERR1211248_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211248_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5256016
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176470.33574859741675067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142020.27020465691124224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94270.1793563794326349No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93600.049.2264181
TACCTGG42000.045.764832
GTATCAA162150.038.8830031
ACCTGGG53250.035.4848373
GTACCTG56100.034.8640981
ATCAACG192600.031.3849223
CAACGCA193900.031.1889485
TATCAAC206800.030.019962
TCAACGC209850.028.9618264
AACGCAG214150.028.8541076
CCTGGGG50200.027.060174
TAACGCA5050.026.9924934
GTACATG183850.026.877391
GTGGTAT39000.026.5219881
GTACTAG26100.026.300281
TACATGG185450.026.177692
ACGCAGA236850.025.5927127
TAACGCC12350.025.1196374
CGCAGAG241450.025.0078268
ACATGGG187450.024.95023
Produced by FastQC (version 0.11.5)