##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211248_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5256016 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14183461389767 33.0 31.0 34.0 30.0 34.0 2 32.26964358555986 34.0 31.0 34.0 30.0 34.0 3 32.35042625441019 34.0 31.0 34.0 30.0 34.0 4 35.97093844463183 37.0 35.0 37.0 35.0 37.0 5 35.85986781623191 37.0 35.0 37.0 35.0 37.0 6 35.89068659608342 37.0 35.0 37.0 35.0 37.0 7 35.82815596451761 37.0 35.0 37.0 35.0 37.0 8 35.8164817230389 37.0 35.0 37.0 35.0 37.0 9 37.418895985095936 39.0 37.0 39.0 34.0 39.0 10-11 37.39688349502741 39.0 37.0 39.0 34.0 39.0 12-13 37.30258146093924 39.0 37.0 39.0 34.0 39.0 14-15 38.52708572424437 40.0 38.0 41.0 34.0 41.0 16-17 38.478297916140285 40.0 38.0 41.0 34.0 41.0 18-19 38.504640111445624 40.0 38.0 41.0 34.0 41.0 20-21 38.46514860304839 40.0 38.0 41.0 34.0 41.0 22-23 38.336023900992686 40.0 38.0 41.0 34.0 41.0 24-25 38.27616925062633 40.0 38.0 41.0 34.0 41.0 26-27 38.138848131360334 40.0 38.0 41.0 33.0 41.0 28-29 38.106329204477305 40.0 37.5 41.0 33.5 41.0 30-31 38.10870771702369 40.0 37.5 41.0 33.5 41.0 32-33 37.96484847078091 40.0 37.0 41.0 33.0 41.0 34-35 37.81324457535898 40.0 37.0 41.0 33.0 41.0 36-37 37.661304874262186 40.0 37.0 41.0 33.0 41.0 38-39 37.570689758174254 40.0 37.0 41.0 33.0 41.0 40-41 37.46740668217144 40.0 36.0 41.0 32.0 41.0 42-43 37.321988650719476 40.0 36.0 41.0 32.0 41.0 44-45 37.18123184937032 40.0 36.0 41.0 31.0 41.0 46-47 36.946484847078096 39.0 36.0 41.0 31.0 41.0 48-49 36.72092151165445 39.0 35.0 41.0 30.0 41.0 50-51 36.526109699818264 39.0 35.0 41.0 30.0 41.0 52-53 36.32593317828561 39.0 35.0 40.0 29.5 41.0 54-55 36.09209893957705 38.5 35.0 40.0 29.0 41.0 56-57 35.84813897065762 38.0 35.0 40.0 28.5 41.0 58-59 35.58547186690451 38.0 34.0 40.0 28.0 41.0 60-61 35.326338523322605 37.5 34.0 40.0 28.0 41.0 62-63 35.02530233926228 37.0 34.0 40.0 27.5 41.0 64-65 34.65379852344437 36.5 34.0 39.5 26.5 41.0 66-67 34.349543741875976 36.0 33.0 39.0 26.0 40.5 68-69 34.018597450997106 35.5 33.0 39.0 26.0 40.0 70-71 33.69101996645368 35.0 33.0 38.0 26.0 40.0 72-73 33.339949212483376 35.0 32.5 37.5 26.0 40.0 74-75 32.995344287384206 35.0 32.0 37.0 25.0 39.0 76-77 31.99672185168386 34.0 30.5 36.0 24.5 38.5 78-79 32.48506235521353 35.0 32.0 36.0 25.0 38.5 80-81 32.40732353173963 35.0 32.0 36.0 25.0 37.5 82-83 32.14888082532474 35.0 32.0 35.0 25.0 37.0 84-85 31.885090532448913 34.0 31.0 35.0 24.0 36.5 86-87 31.63829495572312 34.0 31.0 35.0 24.0 36.0 88-89 31.41337944937763 34.0 31.0 35.0 23.5 36.0 90-91 31.19302909275771 34.0 31.0 35.0 23.0 35.5 92-93 31.007686144791037 34.0 31.0 35.0 22.0 35.0 94-95 30.811273595818584 34.0 30.0 35.0 20.0 35.0 96-97 30.63467291575977 34.0 30.0 35.0 20.0 35.0 98-99 30.453019739665937 34.0 30.0 35.0 20.0 35.0 100 28.695509678813764 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4048983987375614 1101 2 -0.3309409908344847 1101 3 -0.41256494132076327 1101 4 -0.20712680795792693 1101 5 -0.3614352887776562 1101 6 -0.3476293063760778 1101 7 -0.30671871690118735 1101 8 -0.34534452883657707 1101 9 -0.5499416012441714 1101 10-11 -0.49320620080568744 1101 12-13 -0.5122478750941184 1101 14-15 -0.7060393959122422 1101 16-17 -0.5764066204882354 1101 18-19 -0.7660939493230856 1101 20-21 -0.7090976688294077 1101 22-23 -0.8112779349997723 1101 24-25 -0.7696550431898075 1101 26-27 -0.8357029004273784 1101 28-29 -0.9117626202906379 1101 30-31 -0.6864938158358811 1101 32-33 -0.6626709461881788 1101 34-35 -0.7311324142728282 1101 36-37 -0.7509405348856788 1101 38-39 -0.708859273946743 1101 40-41 -0.7813360631972373 1101 42-43 -0.7391908728359127 1101 44-45 -0.8810360699172293 1101 46-47 -0.9067319125894997 1101 48-49 -0.8511791566388496 1101 50-51 -0.9165610384989762 1101 52-53 -0.8619684135602697 1101 54-55 -0.7596955626026372 1101 56-57 -0.7865366082122733 1101 58-59 -0.7769335028941029 1101 60-61 -0.8408409926527014 1101 62-63 -0.9443658986012338 1101 64-65 -0.9510408530924366 1101 66-67 -0.9125024660031116 1101 68-69 -0.9453656755490769 1101 70-71 -1.1040721158338727 1101 72-73 -1.141189074815962 1101 74-75 -1.19066112523371 1101 76-77 -1.063759271723633 1101 78-79 -0.9628185479031579 1101 80-81 -0.8147381143469872 1101 82-83 -0.8724420963909125 1101 84-85 -0.7167168327150257 1101 86-87 -0.9220438848362491 1101 88-89 -0.7983636249436934 1101 90-91 -0.9672179759494988 1101 92-93 -1.0508779933092036 1101 94-95 -0.9694996858784073 1101 96-97 -1.1278146465863692 1101 98-99 -1.163018961888536 1101 100 -1.123423418366503 1102 1 -0.41219051411453833 1102 2 -0.34990014712161965 1102 3 -0.4138265810571724 1102 4 -0.3039953446200414 1102 5 -0.32056875851892386 1102 6 -0.4304176953571286 1102 7 -0.382069398329854 1102 8 -0.37043354532467276 1102 9 -0.5055141250506381 1102 10-11 -0.6117466339963471 1102 12-13 -0.5742725285752002 1102 14-15 -0.7453531975540244 1102 16-17 -0.7778342066635346 1102 18-19 -0.7277149926528494 1102 20-21 -0.7564655818796595 1102 22-23 -0.7176509484542137 1102 24-25 -0.8275043994909979 1102 26-27 -0.8489010459363016 1102 28-29 -0.8722700505515064 1102 30-31 -0.8255605499427574 1102 32-33 -0.8299740177343722 1102 34-35 -0.8051752960852028 1102 36-37 -0.8659741537897006 1102 38-39 -0.9922863304529344 1102 40-41 -0.9033686082515686 1102 42-43 -0.8166182237724371 1102 44-45 -0.8380913903613134 1102 46-47 -0.9301842343502997 1102 48-49 -0.9053543869895364 1102 50-51 -0.9033434170531436 1102 52-53 -0.897947438358095 1102 54-55 -0.8497883826266559 1102 56-57 -0.9336337770984287 1102 58-59 -1.0725663390945996 1102 60-61 -0.9306737039017037 1102 62-63 -0.8985912890364887 1102 64-65 -0.9870109514191796 1102 66-67 -0.9366686190396862 1102 68-69 -1.0440746882489123 1102 70-71 -1.072818678903829 1102 72-73 -1.0028426639638184 1102 74-75 -1.2152027628774107 1102 76-77 -1.1537805367735672 1102 78-79 -0.9011833176899486 1102 80-81 -0.8376698366766462 1102 82-83 -0.8256642373997494 1102 84-85 -0.859384180657603 1102 86-87 -0.8561243781197234 1102 88-89 -0.8528653636484123 1102 90-91 -0.9770937557507402 1102 92-93 -1.139276406822301 1102 94-95 -1.1732885384631295 1102 96-97 -1.3127783354204716 1102 98-99 -1.3245124282845246 1102 100 -1.1406481660958399 1103 1 -0.3642924321235306 1103 2 -0.3802776890824724 1103 3 -0.43400668506089346 1103 4 -0.22974486194694776 1103 5 -0.23708731148613538 1103 6 -0.3128323291597468 1103 7 -0.3766865716271113 1103 8 -0.3624903668695083 1103 9 -0.5808249383371873 1103 10-11 -0.5487093871354887 1103 12-13 -0.591840983598523 1103 14-15 -0.7401700636316235 1103 16-17 -0.697699762829771 1103 18-19 -0.7987051418939615 1103 20-21 -0.748874296771362 1103 22-23 -0.7374928289767624 1103 24-25 -0.7789821542507909 1103 26-27 -0.7620644219703507 1103 28-29 -0.9763887669934022 1103 30-31 -0.8240116273254685 1103 32-33 -0.7710146186447489 1103 34-35 -0.793998560223649 1103 36-37 -0.88020603417133 1103 38-39 -0.8096281757438746 1103 40-41 -0.8525413699872146 1103 42-43 -0.7803971665422083 1103 44-45 -0.7114172545927602 1103 46-47 -0.7851179284553496 1103 48-49 -0.8888190637747257 1103 50-51 -0.905081462966983 1103 52-53 -0.8897841327872769 1103 54-55 -0.8305476943418668 1103 56-57 -0.8494830703522851 1103 58-59 -0.7722159959387014 1103 60-61 -0.8600675792381622 1103 62-63 -0.804349121023975 1103 64-65 -1.0183163531300323 1103 66-67 -0.9846332183153237 1103 68-69 -1.0713710142963464 1103 70-71 -1.0639872410312563 1103 72-73 -0.9035664622135116 1103 74-75 -0.9651793690866342 1103 76-77 -0.9657683043576668 1103 78-79 -0.8402066973880835 1103 80-81 -0.7022247204827892 1103 82-83 -0.7165250161593129 1103 84-85 -0.843889176500273 1103 86-87 -0.8911102150114516 1103 88-89 -0.8413381437045011 1103 90-91 -0.9213725646403823 1103 92-93 -1.062803514025049 1103 94-95 -1.0254090587635893 1103 96-97 -1.140128495371993 1103 98-99 -1.1291979509102674 1103 100 -1.0930215084484658 1104 1 -0.4280480607150672 1104 2 -0.32249760330694954 1104 3 -0.4533919984513055 1104 4 -0.2825666852803508 1104 5 -0.2545075773765859 1104 6 -0.29380177931724916 1104 7 -0.3709567850182012 1104 8 -0.41676372338910994 1104 9 -0.6255184946959886 1104 10-11 -0.5246958170198042 1104 12-13 -0.580992338901865 1104 14-15 -0.6830987761105263 1104 16-17 -0.6854032291538843 1104 18-19 -0.7424405679131425 1104 20-21 -0.7190314076440742 1104 22-23 -0.6746511674917031 1104 24-25 -0.7538724977856219 1104 26-27 -0.7715280402578912 1104 28-29 -0.8254924742906908 1104 30-31 -0.852442636793576 1104 32-33 -0.7338372163721374 1104 34-35 -0.7344585502476306 1104 36-37 -0.7069314864515874 1104 38-39 -0.7376364799804946 1104 40-41 -0.8324824021632864 1104 42-43 -0.8019476855000534 1104 44-45 -0.9168221601508648 1104 46-47 -0.882413516915058 1104 48-49 -0.8545216327128671 1104 50-51 -0.7553145555548895 1104 52-53 -0.7771597343661298 1104 54-55 -0.9596018176481707 1104 56-57 -0.7960760784856973 1104 58-59 -0.8737385228318075 1104 60-61 -0.7913125086658184 1104 62-63 -0.7906756237468784 1104 64-65 -0.8653398332110243 1104 66-67 -0.8641794462194738 1104 68-69 -0.8884952794761816 1104 70-71 -0.9233536273217453 1104 72-73 -0.9414187943920851 1104 74-75 -0.9065035827920376 1104 76-77 -0.8375906583787085 1104 78-79 -0.6966586063831244 1104 80-81 -0.648174526087125 1104 82-83 -0.696620175513651 1104 84-85 -0.6455467668139683 1104 86-87 -0.7120218817122819 1104 88-89 -0.8210599996079999 1104 90-91 -0.8324627747061335 1104 92-93 -0.8287588314924186 1104 94-95 -0.7272393844873584 1104 96-97 -0.8532696651168727 1104 98-99 -1.0111420214438915 1104 100 -0.9711209178591744 1105 1 -0.43385706292991344 1105 2 -0.3898884606586783 1105 3 -0.4468405647980802 1105 4 -0.2666852701983231 1105 5 -0.34931515514717404 1105 6 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68-69 -1.1367407475703217 1105 70-71 -1.305021900574431 1105 72-73 -1.2257694816007287 1105 74-75 -1.1936340580411766 1105 76-77 -1.1137565937763227 1105 78-79 -0.9878903497942844 1105 80-81 -1.0021255583020299 1105 82-83 -0.9989893649943298 1105 84-85 -0.967136164533354 1105 86-87 -0.9773996606476416 1105 88-89 -1.1185064225998183 1105 90-91 -1.053011776199856 1105 92-93 -1.0808565624188908 1105 94-95 -1.050297012245057 1105 96-97 -1.19884518834699 1105 98-99 -1.1199742491253453 1105 100 -1.3473996199148495 1106 1 -0.40072668612646467 1106 2 -0.37929120310922215 1106 3 -0.3744246391234789 1106 4 -0.2342415096908539 1106 5 -0.2929111666811792 1106 6 -0.403544848345021 1106 7 -0.386132226205099 1106 8 -0.40551818802592265 1106 9 -0.4857207737649816 1106 10-11 -0.47655447067938894 1106 12-13 -0.5323372872921439 1106 14-15 -0.6175731989573165 1106 16-17 -0.5982860110955599 1106 18-19 -0.6926854014508521 1106 20-21 -0.6988153614058987 1106 22-23 -0.7381977745877819 1106 24-25 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2310 24-25 0.9435666357781756 2310 26-27 0.9827287545627428 2310 28-29 0.9721101208776233 2310 30-31 0.8666632569896251 2310 32-33 0.9846564260698756 2310 34-35 1.0353412633896184 2310 36-37 0.8920713457937168 2310 38-39 0.9942608787748384 2310 40-41 1.0660120311760437 2310 42-43 1.0482131510514776 2310 44-45 1.0537666216148978 2310 46-47 0.7761783206615434 2310 48-49 1.000523813450208 2310 50-51 1.0563109658829646 2310 52-53 1.0977050576081169 2310 54-55 1.245364188182613 2310 56-57 1.2085563302725362 2310 58-59 1.1626736494267718 2310 60-61 1.1468955949279191 2310 62-63 0.6189195624718877 2310 64-65 0.8015825343796195 2310 66-67 1.1267437256518136 2310 68-69 1.3423542293150135 2310 70-71 1.3551424796445843 2310 72-73 1.3497846868659806 2310 74-75 1.3294637648946477 2310 76-77 1.3618113741277398 2310 78-79 1.183154010037974 2310 80-81 1.0741495402811907 2310 82-83 1.1283500296900328 2310 84-85 1.1537008734235705 2310 86-87 1.147592722779791 2310 88-89 1.1632126049724185 2310 90-91 1.2672329647456628 2310 92-93 1.2988184054860845 2310 94-95 1.3531872736500539 2310 96-97 1.3720861861760625 2310 98-99 1.414447212186925 2310 100 1.6569932536860286 2311 1 0.3719091610620566 2311 2 0.2952666493248657 2311 3 0.4353200231442713 2311 4 0.2870356412473072 2311 5 0.4063393520257108 2311 6 0.4486888212990152 2311 7 0.5418958612671787 2311 8 0.5261412286821923 2311 9 0.6756842500846147 2311 10-11 0.7052320383186768 2311 12-13 0.7564743409538934 2311 14-15 0.8816450111639682 2311 16-17 0.727364516687544 2311 18-19 0.8707869316134165 2311 20-21 0.771169082044004 2311 22-23 0.9368763378870781 2311 24-25 0.9670396140274633 2311 26-27 0.4113622415414966 2311 28-29 0.7082454049970366 2311 30-31 0.9237371436080934 2311 32-33 1.0168248534482132 2311 34-35 1.0990550454760637 2311 36-37 1.087554041544415 2311 38-39 1.1156874380006343 2311 40-41 1.0955815103661237 2311 42-43 1.1142401602711587 2311 44-45 1.1288708859020176 2311 46-47 0.8666085483961368 2311 48-49 1.1133159626469151 2311 50-51 1.2614350867083388 2311 52-53 1.0945132618547788 2311 54-55 1.2421262809979297 2311 56-57 1.3177830126721375 2311 58-59 1.3030415164834253 2311 60-61 1.3661090382658472 2311 62-63 1.3190047186584408 2311 64-65 1.387446456765602 2311 66-67 1.3753297125151889 2311 68-69 1.4369251184165321 2311 70-71 1.3930727268947152 2311 72-73 1.4470705491175622 2311 74-75 1.4498302268331287 2311 76-77 1.3713303101492613 2311 78-79 1.1554312340996802 2311 80-81 1.095201469805616 2311 82-83 1.162998079773594 2311 84-85 1.2574955278665279 2311 86-87 1.2974543405528678 2311 88-89 1.2974824783214238 2311 90-91 1.323454577352944 2311 92-93 1.3558462217585294 2311 94-95 1.3861378560824882 2311 96-97 1.428606420630846 2311 98-99 1.4820224397619732 2311 100 1.6121282141900188 2312 1 0.3122086274479301 2312 2 0.38063380399761115 2312 3 0.4653162356936278 2312 4 0.22348539208857687 2312 5 0.3755642333952238 2312 6 0.44674750497153326 2312 7 0.3784263185175689 2312 8 0.4520118961658355 2312 9 0.6799123141024452 2312 10-11 0.6599563376898772 2312 12-13 0.7052505581433621 2312 14-15 0.8471260669703753 2312 16-17 0.8622963128314822 2312 18-19 0.8195098072204487 2312 20-21 0.7968523599536468 2312 22-23 0.9078285743667678 2312 24-25 0.8705893079578289 2312 26-27 0.9496057766011887 2312 28-29 0.9449614671588122 2312 30-31 0.9586082612233113 2312 32-33 0.7747183170222556 2312 34-35 0.8749787570983827 2312 36-37 0.9485413169000125 2312 38-39 0.7755134928849614 2312 40-41 0.8675551687460015 2312 42-43 0.9843068075048365 2312 44-45 0.7258236736441575 2312 46-47 0.8400601057125598 2312 48-49 0.95433008725999 2312 50-51 0.8178526343042947 2312 52-53 0.9517924048994573 2312 54-55 0.8828840090827654 2312 56-57 0.8614501374022083 2312 58-59 0.9620024065938111 2312 60-61 1.0175249697506672 2312 62-63 1.0507064737749658 2312 64-65 1.0703789350472448 2312 66-67 1.068577937271634 2312 68-69 1.1173279365970927 2312 70-71 1.0319631030024397 2312 72-73 1.0249456901554836 2312 74-75 1.0297184672946003 2312 76-77 1.0454887921549059 2312 78-79 0.9429322503658852 2312 80-81 0.8716784268368869 2312 82-83 0.9548346364058986 2312 84-85 0.9879589254278862 2312 86-87 1.0653550022661022 2312 88-89 1.0093219300730674 2312 90-91 1.1293929430382441 2312 92-93 1.0934746431054734 2312 94-95 1.102463949634501 2312 96-97 0.8455327557072003 2312 98-99 0.6469684933772051 2312 100 0.8992506836871605 2313 1 0.06355035429006506 2313 2 0.2321553734850923 2313 3 0.31378689585876174 2313 4 0.19426267476231374 2313 5 0.3319106518401824 2313 6 0.2898365605650497 2313 7 0.25606944435450885 2313 8 0.3945068780294889 2313 9 0.6027928176618502 2313 10-11 0.3903233792502476 2313 12-13 0.4381548481674926 2313 14-15 0.5673538020495812 2313 16-17 0.7754830396467725 2313 18-19 0.7455660440872194 2313 20-21 0.7105334751211885 2313 22-23 0.6716317402210024 2313 24-25 0.5477255847703191 2313 26-27 0.7721315663585955 2313 28-29 0.6313875648929184 2313 30-31 0.8650275777956935 2313 32-33 0.9098292962419379 2313 34-35 0.9623820910374903 2313 36-37 0.9936768621751924 2313 38-39 1.0171536087347022 2313 40-41 1.0071106333067021 2313 42-43 1.0299967824020158 2313 44-45 0.8826981817671111 2313 46-47 0.9008184839490454 2313 48-49 0.9908361249988289 2313 50-51 0.9145278074565226 2313 52-53 0.9612118040317839 2313 54-55 0.9498438146604968 2313 56-57 0.6621566383457704 2313 58-59 0.7483887707588082 2313 60-61 0.6704378276051983 2313 62-63 0.9620639611309016 2313 64-65 1.0666187003623122 2313 66-67 1.0577316953209035 2313 68-69 1.0554180900077768 2313 70-71 0.9559243110767497 2313 72-73 1.0577762513407052 2313 74-75 1.1281726026913788 2313 76-77 1.1021947169681 2313 78-79 0.9942385377782585 2313 80-81 0.8849056029111679 2313 82-83 0.9471921674700283 2313 84-85 0.9382563456981181 2313 86-87 0.9235059177484999 2313 88-89 1.0124732149025668 2313 90-91 0.9031699632071515 2313 92-93 1.0674131663662898 2313 94-95 1.196565565841194 2313 96-97 1.2332893209478293 2313 98-99 1.2615596849216324 2313 100 1.3468598832672534 2314 1 -0.31137939397806136 2314 2 0.08317891175975234 2314 3 0.3410182546341076 2314 4 0.28084980668218407 2314 5 0.35046185242081407 2314 6 0.40972930459469836 2314 7 0.4728591988459243 2314 8 0.4502313306651402 2314 9 0.4712307466867287 2314 10-11 0.6061927961099443 2314 12-13 0.5192613713628447 2314 14-15 0.8338986840686147 2314 16-17 0.8910197269285973 2314 18-19 0.8704893263999125 2314 20-21 0.901633389560871 2314 22-23 0.9077383742613065 2314 24-25 0.8689351767929949 2314 26-27 0.7456463388947725 2314 28-29 0.7995463693572304 2314 30-31 0.8846345765258761 2314 32-33 0.8905000589790291 2314 34-35 0.9219893174491958 2314 36-37 0.910172967423172 2314 38-39 0.7023846825087077 2314 40-41 0.7036588017287144 2314 42-43 0.8969982707201538 2314 44-45 0.8935908201445528 2314 46-47 0.8265404590502712 2314 48-49 0.8670037417365393 2314 50-51 0.8073640581091084 2314 52-53 0.8297307697026 2314 54-55 0.7564325583258622 2314 56-57 0.8048594007217602 2314 58-59 0.9585095038773161 2314 60-61 0.9952483576357878 2314 62-63 0.9545170427603153 2314 64-65 0.9920796156340259 2314 66-67 0.6203494056676675 2314 68-69 0.6587378413396152 2314 70-71 0.8145602261587328 2314 72-73 0.942807469672502 2314 74-75 1.0467907646064845 2314 76-77 0.8938039389883414 2314 78-79 0.7391239171096444 2314 80-81 0.7543561904293554 2314 82-83 0.7922228884974558 2314 84-85 0.8441495822388454 2314 86-87 0.867580246577333 2314 88-89 0.8704771390135129 2314 90-91 0.9705314379762449 2314 92-93 0.8825615867558483 2314 94-95 0.9200632622634366 2314 96-97 0.8843130189989985 2314 98-99 1.0474161788579153 2314 100 1.1173799797562864 2315 1 0.4426070696629907 2315 2 0.4765424675947614 2315 3 0.4371392059360346 2315 4 0.29136999555057486 2315 5 0.36535980575339266 2315 6 0.4494363035210398 2315 7 0.43928444140696143 2315 8 0.29500749433525897 2315 9 0.5255186444383853 2315 10-11 0.59356125852905 2315 12-13 0.4506057981437195 2315 14-15 0.6135610017400239 2315 16-17 0.772073273252964 2315 18-19 0.7400461371894593 2315 20-21 0.49975871951703965 2315 22-23 0.8648568262997642 2315 24-25 0.8822011446940721 2315 26-27 0.8558481138859264 2315 28-29 0.763718592139675 2315 30-31 0.8179109398997966 2315 32-33 0.8186638072019576 2315 34-35 0.8441494947308072 2315 36-37 0.864952907670208 2315 38-39 0.8983712539107316 2315 40-41 0.8924865601622471 2315 42-43 0.9522237382939878 2315 44-45 0.9407862916135983 2315 46-47 0.9123856362033678 2315 48-49 0.7631694286681991 2315 50-51 0.868839661619937 2315 52-53 0.8928082547791902 2315 54-55 0.7108191216210926 2315 56-57 0.6877630001356962 2315 58-59 0.7451674606898919 2315 60-61 0.8728785902215179 2315 62-63 0.9539182032173841 2315 64-65 1.1089578991303881 2315 66-67 1.0651669930479741 2315 68-69 1.0170365478125873 2315 70-71 0.8995535973764746 2315 72-73 1.0147126044330221 2315 74-75 0.46683764062601085 2315 76-77 0.46984272008330663 2315 78-79 0.7383981371278807 2315 80-81 0.7254387086350178 2315 82-83 0.6863205041131053 2315 84-85 0.7320763008284992 2315 86-87 0.7913419346874377 2315 88-89 0.8585449354896291 2315 90-91 0.8608974100295761 2315 92-93 0.8989132256122794 2315 94-95 0.9378479489360778 2315 96-97 1.0615964639489626 2315 98-99 1.0660339615920407 2315 100 0.972841977703883 2316 1 0.312070193015515 2316 2 0.1002336534867112 2316 3 0.2503254010954379 2316 4 0.04527553108233917 2316 5 0.33111203458862803 2316 6 0.38360411537458106 2316 7 0.4445683580927806 2316 8 0.4284045668952814 2316 9 0.6349902772221867 2316 10-11 0.5973660502191507 2316 12-13 0.38118864528205876 2316 14-15 0.5368786773937586 2316 16-17 0.8369614769315845 2316 18-19 0.7759710976988501 2316 20-21 0.7735079798322744 2316 22-23 0.760141916700249 2316 24-25 0.4639520824431784 2316 26-27 0.7052310578557197 2316 28-29 0.9215257508210115 2316 30-31 0.5553474248200132 2316 32-33 0.6599869993145973 2316 34-35 0.8045387034025993 2316 36-37 0.8329521887699798 2316 38-39 0.7380503896655028 2316 40-41 0.748785862291065 2316 42-43 0.7263896673135903 2316 44-45 0.6527210135165831 2316 46-47 0.7732076521561027 2316 48-49 0.8483016427341923 2316 50-51 0.8308942002278172 2316 52-53 0.8496770530420932 2316 54-55 0.7442500607395317 2316 56-57 0.8129811154433852 2316 58-59 0.7442693244509613 2316 60-61 0.8320829583962421 2316 62-63 0.854405050277478 2316 64-65 0.9090153622439487 2316 66-67 0.862395803830502 2316 68-69 0.9440302733296377 2316 70-71 0.8935089668765244 2316 72-73 0.9472249895679283 2316 74-75 0.9208343835678363 2316 76-77 0.7774866590697087 2316 78-79 0.5457719994804862 2316 80-81 0.5432171846643499 2316 82-83 0.44040001839086074 2316 84-85 0.6367676138728733 2316 86-87 0.7135533842739505 2316 88-89 0.6687082040893451 2316 90-91 0.4693634383258818 2316 92-93 0.3653415629965586 2316 94-95 0.3880675313870867 2316 96-97 0.6442984773586531 2316 98-99 0.7535148275978436 2316 100 0.4149597501890767 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 5.0 12 30.0 13 131.0 14 518.0 15 1543.0 16 3638.0 17 7550.0 18 12856.0 19 18596.0 20 25193.0 21 33984.0 22 41740.0 23 43052.0 24 43151.0 25 45331.0 26 52992.0 27 64508.0 28 79162.0 29 98613.0 30 121405.0 31 152102.0 32 192709.0 33 252713.0 34 357495.0 35 498487.0 36 770708.0 37 1168496.0 38 1013383.0 39 155907.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.276877453051775 19.653194154170617 13.214635130556601 23.855293262221004 2 15.702121150316135 20.16447438516169 40.51464074690793 23.618763717614254 3 19.086414501021306 25.68917217908012 29.552326324729606 25.672086995168964 4 12.479945914638545 16.461089887792287 38.23219894794684 32.82676524962233 5 13.025245737455899 37.416381533085136 34.574152742305195 14.984219987153768 6 30.659609864201325 36.83274556241838 18.190051171838135 14.317593401542156 7 27.519566786206855 32.14341093456911 22.187918393035886 18.149103886188154 8 26.197713248970324 35.45761656737727 19.953972742853143 18.39069744079927 9 26.297671744054156 16.066239004390347 20.92675086335123 36.70933838820427 10-11 25.435103567548644 26.186950507896707 27.652516462169828 20.725429462384824 12-13 25.570065914956487 23.775502923793027 28.219145112789825 22.435286048460668 14-15 22.753626538607563 25.294431965080737 26.209517656024467 25.74242384028723 16-17 21.949437575889487 27.882164338723115 27.180813234708236 22.987584850679166 18-19 21.463701924014494 27.314588398759476 29.436823388363297 21.784886288862733 20-21 22.55601224920284 26.610918479977848 29.188079829673256 21.644989441146052 22-23 22.50348933488787 26.428401283405528 28.936527209962833 22.13158217174377 24-25 22.055992723725602 26.360027751212833 29.523972331359676 22.060007193701885 26-27 22.04759809645666 26.62889704360979 28.982964666487504 22.340540193446046 28-29 21.645232947162672 27.054688542705257 29.174671392812364 22.12540711731971 30-31 22.43550453134268 26.654369357820766 29.152574943266057 21.757551167570494 32-33 22.382829480969633 26.771925715009353 28.768836009558925 22.07640879446209 34-35 22.411179641241628 26.807527390642488 29.08047827598669 21.700814692129196 36-37 22.272876326062118 26.66221069573858 28.862121132245466 22.202791845953836 38-39 22.223990964507433 26.902218101147845 29.02396032604274 21.849830608301975 40-41 22.303136883558043 26.256048146585897 29.289601173831787 22.151213796024276 42-43 22.193958289474825 26.557799578634867 28.774337727381305 22.47390440450901 44-45 22.050785029589527 27.04801930659224 28.799244283078362 22.101951380739873 46-47 22.09350429698992 26.75316275279853 28.376489084644668 22.776843865566878 48-49 22.377881429377332 26.78883835265623 28.42567003846187 22.407610179504573 50-51 22.257120575390577 26.949094378062732 28.268170307557632 22.52561473898906 52-53 22.064706936164256 27.29164016631549 27.95256566370786 22.691087233812397 54-55 21.97593007450605 27.09848834916015 28.141333250372302 22.784248325961503 56-57 22.400473821162002 26.98969357161251 27.8040213725612 22.805811234664287 58-59 21.962743168061788 27.14806739436404 28.22672237641833 22.662467061155844 60-61 22.258335922547705 27.476080130492598 27.82150955034812 22.444074396611576 62-63 22.27399851729789 27.14431250177775 27.76997550107312 22.811713479851242 64-65 21.91512751351874 27.04716694568219 27.87452599716645 23.163179543632623 66-67 22.002199183339968 26.917465326977176 27.85057622905926 23.229759260623595 68-69 21.774217267866003 26.99548375901794 27.995182879347063 23.235116093768994 70-71 21.857671406295758 26.780577917299876 28.000632741578457 23.361117934825902 72-73 22.05850246258536 26.984234695091487 27.897042518261202 23.06022032406196 74-75 22.183003495712246 27.144804174832434 27.597265476382386 23.074926853072935 76-77 22.014709099565096 27.009678521663837 27.52376523411528 23.451847144655783 78-79 21.85424950294664 27.07413513857316 27.659762325862445 23.411853032617756 80-81 21.666728184107875 27.313382402688603 27.686520564646393 23.33336884855712 82-83 22.185298051336396 26.889523071040394 28.11764193108846 22.80753694653475 84-85 22.317908692512134 27.125700936892173 27.469854336091075 23.08653603450462 86-87 21.658263748976424 27.416847379606065 27.68620587349812 23.238682997919394 88-89 21.964454227147083 26.806530290367874 27.70639338985973 23.522622092625316 90-91 22.04876629972148 26.81261354413012 27.739582873319396 23.399037282829006 92-93 22.065793779970925 26.969307738478115 27.70459310919896 23.260305372352004 94-95 21.46899707668048 26.943527966956193 27.900442731727253 23.68703222463607 96-97 21.562425558827268 26.946534399527533 27.73738980803786 23.753650233607342 98-99 21.31787813929466 27.142216368529898 27.58189983525548 23.958005656919966 100 21.464490363306798 26.893632862429417 27.507017120479794 24.13485965378399 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1318.0 1 942.0 2 1450.0 3 2204.5 4 1515.5 5 1045.5 6 1543.0 7 2719.5 8 4119.5 9 4691.5 10 4113.5 11 3543.0 12 3683.0 13 4176.5 14 4748.0 15 5367.0 16 6085.5 17 6866.0 18 7649.0 19 8385.0 20 9213.5 21 10270.0 22 11883.0 23 13961.5 24 16407.5 25 19502.0 26 23991.0 27 29500.5 28 35563.0 29 44185.0 30 55574.5 31 66734.5 32 79785.5 33 93421.5 34 105557.0 35 118440.0 36 130751.0 37 146891.0 38 156255.0 39 155481.0 40 153831.5 41 151910.0 42 153909.5 43 160188.5 44 170203.5 45 186735.5 46 200994.0 47 210237.5 48 218131.5 49 219331.5 50 215610.0 51 215383.5 52 212840.5 53 202749.0 54 191852.5 55 174126.0 56 154295.0 57 132705.0 58 111832.5 59 99120.5 60 80982.5 61 61048.5 62 47219.5 63 36907.0 64 27197.0 65 18035.5 66 12448.0 67 9139.5 68 7042.0 69 5277.0 70 4083.5 71 3137.0 72 2397.5 73 1884.0 74 1466.0 75 1074.0 76 685.5 77 405.5 78 251.5 79 165.5 80 110.5 81 66.5 82 44.0 83 33.0 84 19.0 85 10.5 86 6.5 87 4.0 88 2.0 89 2.0 90 3.0 91 3.0 92 1.5 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044824825495203974 2 0.0 3 0.0 4 0.03993519045604123 5 0.0 6 0.0 7 8.181101427392915E-4 8 0.0 9 0.011853084161083223 10-11 4.946712490981762E-4 12-13 1.902581727300678E-5 14-15 1.0464199500153727E-4 16-17 3.42464710914122E-4 18-19 9.51290863650339E-6 20-21 9.51290863650339E-6 22-23 0.0 24-25 5.612616095537E-4 26-27 5.422357922806933E-4 28-29 1.2366781227454406E-4 30-31 0.0023496884332163373 32-33 0.015648734707048074 34-35 0.02650296346129844 36-37 0.03125941777955014 38-39 0.022517054742603522 40-41 0.0012747297572914542 42-43 8.56161777285305E-5 44-45 0.011586722719261129 46-47 0.017922319871172387 48-49 0.0030631565809540913 50-51 0.01131084836880253 52-53 0.01108253856152645 54-55 0.010207350966968138 56-57 4.6613252318866607E-4 58-59 0.008428437051942003 60-61 0.00454717032824862 62-63 0.0013603459350199846 64-65 0.0347791939750564 66-67 0.060064505130882406 68-69 0.05870415919586242 70-71 0.051112858103932714 72-73 0.04549072909975921 74-75 0.03548314921415764 76-77 0.031383085591824685 78-79 0.015135037640676892 80-81 0.0010273941327423662 82-83 0.03814676363237859 84-85 0.05117944846438824 86-87 0.0566874225649237 88-89 0.06966302994511432 90-91 0.048553885680713296 92-93 0.03937392884648753 94-95 0.013108788101101672 96-97 0.0035768536473252746 98-99 0.023078316352157225 100 0.032800508978663684 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5256016.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.04101017002743 #Duplication Level Percentage of deduplicated Percentage of total 1 80.61160877675856 56.46118510155219 2 13.059842420781878 18.294491116258786 3 3.314609063081518 6.964757012908731 4 1.2347579075584427 3.4593476464329065 5 0.5492234015767227 1.9234080927726143 6 0.319662802879637 1.3433703376483277 7 0.20193496955232298 0.9900610479268904 8 0.13899881077684229 0.7788493695394023 9 0.0991754492140265 0.6251713782315004 >10 0.43682114675394557 5.1980118037260645 >50 0.021778256729847324 1.0418009160691992 >100 0.010770363224102877 1.388343378892745 >500 4.0831581550397503E-4 0.1876524287005704 >1k 3.140886897375273E-4 0.43551596715242813 >5k 3.14088689734938E-5 0.20738541305896901 >10k+ 6.28177379469876E-5 0.700648989128578 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17647 0.33574859741675067 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14202 0.27020465691124224 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9427 0.1793563794326349 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.902581727300678E-5 2 0.0 0.0 0.0 1.902581727300678E-5 3.805163454601356E-5 3 0.0 0.0 0.0 1.902581727300678E-5 3.805163454601356E-5 4 0.0 0.0 0.0 1.902581727300678E-5 7.610326909202712E-5 5 0.0 0.0 0.0 1.902581727300678E-5 7.610326909202712E-5 6 0.0 0.0 0.0 1.902581727300678E-5 7.610326909202712E-5 7 0.0 0.0 0.0 1.902581727300678E-5 7.610326909202712E-5 8 0.0 0.0 0.0 1.902581727300678E-5 7.610326909202712E-5 9 0.0 0.0 0.0 3.805163454601356E-5 7.610326909202712E-5 10-11 0.0 0.0 0.0 7.610326909202712E-5 7.610326909202712E-5 12-13 0.0 0.0 0.0 9.51290863650339E-5 7.610326909202712E-5 14-15 0.0 0.0 0.0 1.2366781227454406E-4 1.1415490363804067E-4 16-17 0.0 0.0 0.0 1.617194468205576E-4 1.1415490363804067E-4 18-19 0.0 0.0 0.0 1.71232355457061E-4 1.902581727300678E-4 20-21 0.0 0.0 0.0 2.5684853318559155E-4 1.902581727300678E-4 22-23 0.0 0.0 0.0 6.183390613727204E-4 2.0928399000307458E-4 24-25 0.0 0.0 0.0 0.0012271652141089373 2.2830980727608135E-4 26-27 0.0 0.0 0.0 0.0022069948036687864 2.2830980727608135E-4 28-29 0.0 0.0 0.0 0.005983619532360632 2.2830980727608135E-4 30-31 0.0 0.0 0.0 0.019901004867565093 2.2830980727608135E-4 32-33 0.0 0.0 0.0 0.05155996480984837 2.2830980727608135E-4 34-35 0.0 0.0 0.0 0.09640381612232535 2.2830980727608135E-4 36-37 0.0 0.0 0.0 0.1711467392793325 2.2830980727608135E-4 38-39 0.0 0.0 0.0 0.28496869111509554 2.3782271591258475E-4 40-41 0.0 0.0 0.0 0.40879822283646017 2.473356245490881E-4 42-43 0.0 0.0 0.0 0.529041388001863 2.6636144182209487E-4 44-45 0.0 0.0 0.0 0.6681010864502696 2.8538725909510167E-4 46-47 0.0 0.0 0.0 0.8307242595912949 2.8538725909510167E-4 48-49 0.0 0.0 0.0 1.0036118611511076 3.2343889364111527E-4 50-51 0.0 0.0 0.0 1.1827399307764663 3.2343889364111527E-4 52-53 0.0 0.0 0.0 1.3548189351021762 3.4246471091412206E-4 54-55 0.0 0.0 0.0 1.5476741318900094 3.614905281871288E-4 56-57 9.51290863650339E-6 0.0 0.0 1.7646065004368328 3.614905281871288E-4 58-59 1.902581727300678E-5 0.0 0.0 2.009944414172255 3.614905281871288E-4 60-61 1.902581727300678E-5 0.0 0.0 2.255558202258136 3.614905281871288E-4 62-63 1.902581727300678E-5 0.0 0.0 2.505776238124085 3.805163454601356E-4 64-65 1.902581727300678E-5 0.0 0.0 2.75796344607779 3.805163454601356E-4 66-67 1.902581727300678E-5 0.0 0.0 3.04052537130785 3.805163454601356E-4 68-69 1.902581727300678E-5 0.0 0.0 3.3565251703952192 3.805163454601356E-4 70-71 1.902581727300678E-5 0.0 0.0 3.6874031585900804 3.805163454601356E-4 72-73 1.902581727300678E-5 0.0 0.0 4.00963391283436 4.946712490981762E-4 74-75 3.805163454601356E-5 0.0 0.0 4.3245968048803505 4.946712490981762E-4 76-77 3.805163454601356E-5 0.0 0.0 4.675404336668686 4.946712490981762E-4 78-79 3.805163454601356E-5 0.0 0.0 5.062579718174374 4.946712490981762E-4 80-81 3.805163454601356E-5 0.0 0.0 5.465670195828932 5.232099750076864E-4 82-83 3.805163454601356E-5 0.0 0.0 5.8796910055068325 5.898003354632102E-4 84-85 3.805163454601356E-5 0.0 0.0 6.278396032279963 5.898003354632102E-4 86-87 3.805163454601356E-5 0.0 0.0 6.694072088060615 5.898003354632102E-4 88 3.805163454601356E-5 0.0 0.0 7.031295186316023 5.898003354632102E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9360 0.0 49.226418 1 TACCTGG 4200 0.0 45.76483 2 GTATCAA 16215 0.0 38.883003 1 ACCTGGG 5325 0.0 35.484837 3 GTACCTG 5610 0.0 34.864098 1 ATCAACG 19260 0.0 31.384922 3 CAACGCA 19390 0.0 31.188948 5 TATCAAC 20680 0.0 30.01996 2 TCAACGC 20985 0.0 28.961826 4 AACGCAG 21415 0.0 28.854107 6 CCTGGGG 5020 0.0 27.06017 4 TAACGCA 505 0.0 26.992493 4 GTACATG 18385 0.0 26.87739 1 GTGGTAT 3900 0.0 26.521988 1 GTACTAG 2610 0.0 26.30028 1 TACATGG 18545 0.0 26.17769 2 ACGCAGA 23685 0.0 25.592712 7 TAACGCC 1235 0.0 25.119637 4 CGCAGAG 24145 0.0 25.007826 8 ACATGGG 18745 0.0 24.9502 3 >>END_MODULE