FastQCFastQC Report
Mon 6 Jun 2016
ERR1211247_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211247_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7611443
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301200.3957199705758816No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232990.3061049002140593No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144490.18983259810261996No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC90080.11834812400224241No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA165400.044.9289251
GTATCAA291250.040.381251
TACCTGG41700.035.2125852
CAACGCA330700.033.5608335
GTACTAG78850.033.3051871
ATCAACG335450.033.2397583
GGGCACC77650.032.92434394
ACCTGGG44150.032.5807953
GCATCGT76650.032.3173078
TATCAAC366050.031.8724582
TAGGCAT93950.030.821585
AACGCAG378250.030.1621156
CTAACGC39050.028.8909053
TAACGCC39400.028.753574
CTGTGCG101300.028.5831999
TCAACGC398350.028.4159774
TCTAACG39900.028.3085582
GTACATG404850.028.1436251
CTAGGCA106350.028.0235234
ACGCAGA399300.027.7718817
Produced by FastQC (version 0.11.5)