FastQCFastQC Report
Mon 6 Jun 2016
ERR1211247_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211247_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7611443
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277470.36454322787413634No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228810.3006131688826941No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143420.18842681998669633No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC90910.1194385874005757No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA171800.043.4046551
TACCTGG44100.039.4311642
GTATCAA295500.039.188911
ACCTGGG47000.036.0041433
GGGCACC81100.034.5492794
GTACTAG79800.034.1728671
CAACGCA344050.032.095835
ATCAACG349750.031.5833323
TATCAAC382300.030.118842
CCTGGGG45700.029.725524
CTGTGCG105300.029.6755249
TAGGCAT96200.029.64945
CTGGGGG40650.029.0145245
AACGCAG390600.029.0046236
TAGTACT112600.028.0529614
ACTGTGC117700.027.586968
TCAACGC406050.027.586234
GTGGTAT59600.027.3740791
GTACCTG63650.027.2573911
GCATCGT94100.026.8654968
Produced by FastQC (version 0.11.5)