##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211247_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7611443 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18086005505132 33.0 31.0 34.0 30.0 34.0 2 32.320452376770085 34.0 31.0 34.0 31.0 34.0 3 32.39442796852055 34.0 31.0 34.0 31.0 34.0 4 36.00346491460292 37.0 35.0 37.0 35.0 37.0 5 35.89854328541907 37.0 35.0 37.0 35.0 37.0 6 35.928080917113874 37.0 35.0 37.0 35.0 37.0 7 35.870518376081904 37.0 35.0 37.0 35.0 37.0 8 35.862715913395135 37.0 35.0 37.0 35.0 37.0 9 37.46285099947539 39.0 37.0 39.0 35.0 39.0 10-11 37.44201355774457 39.0 37.0 39.0 34.5 39.0 12-13 37.32985703236561 39.0 37.0 39.0 34.0 39.0 14-15 38.53423476993784 40.0 38.0 41.0 34.0 41.0 16-17 38.49628578969849 40.0 38.0 41.0 34.0 41.0 18-19 38.53051990273066 40.0 38.0 41.0 34.0 41.0 20-21 38.49649908696682 40.0 38.0 41.0 34.0 41.0 22-23 38.36027097621305 40.0 38.0 41.0 34.0 41.0 24-25 38.28800241951493 40.0 38.0 41.0 34.0 41.0 26-27 38.14020711972749 40.0 37.5 41.0 33.5 41.0 28-29 38.126118923836124 40.0 37.5 41.0 33.5 41.0 30-31 38.12552363592554 40.0 37.0 41.0 34.0 41.0 32-33 37.98199658067465 40.0 37.0 41.0 33.0 41.0 34-35 37.840057791932495 40.0 37.0 41.0 33.0 41.0 36-37 37.70392650907325 40.0 36.5 41.0 33.0 41.0 38-39 37.604193909091876 40.0 36.0 41.0 33.0 41.0 40-41 37.493261724485095 40.0 36.0 41.0 32.5 41.0 42-43 37.33212999164547 40.0 36.0 41.0 32.0 41.0 44-45 37.145284212205226 39.0 36.0 41.0 31.5 41.0 46-47 36.86477971391233 39.0 35.0 41.0 31.0 41.0 48-49 36.5731220742243 39.0 35.0 41.0 30.0 41.0 50-51 36.3404233599332 39.0 35.0 40.5 30.0 41.0 52-53 36.112253944488586 38.5 35.0 40.0 29.5 41.0 54-55 35.85451792780948 38.0 35.0 40.0 29.0 41.0 56-57 35.56384728887808 38.0 34.5 40.0 28.5 41.0 58-59 35.25789301713223 37.0 34.0 40.0 28.0 41.0 60-61 34.9841581287543 37.0 34.0 40.0 28.0 41.0 62-63 34.66333827895709 36.0 34.0 39.0 27.0 41.0 64-65 34.2863926065005 35.5 33.0 39.0 26.0 40.5 66-67 33.99012381752054 35.0 33.0 39.0 26.0 40.0 68-69 33.680518398416694 35.0 33.0 38.0 26.0 40.0 70-71 33.4065390097515 35.0 33.0 37.5 26.0 40.0 72-73 33.10260524843975 35.0 32.0 37.0 25.0 39.5 74-75 32.78850344146307 35.0 32.0 36.0 25.0 39.0 76-77 31.82634692265317 34.0 30.5 35.0 24.0 38.0 78-79 32.34354812878452 35.0 32.0 36.0 25.0 38.0 80-81 32.30192914799467 35.0 32.0 35.0 25.0 37.0 82-83 32.07681368697105 35.0 32.0 35.0 25.0 37.0 84-85 31.851965258098893 34.0 31.5 35.0 24.0 36.5 86-87 31.63839938103721 34.0 31.0 35.0 24.0 36.0 88-89 31.445335069841555 34.0 31.0 35.0 24.0 36.0 90-91 31.24049349118163 34.0 31.0 35.0 23.0 35.0 92-93 31.056921860940164 34.0 31.0 35.0 22.5 35.0 94-95 30.881385763514224 34.0 30.5 35.0 20.5 35.0 96-97 30.724095089459382 34.0 30.0 35.0 20.0 35.0 98-99 30.56656687831729 34.0 30.0 35.0 20.0 35.0 100 28.729648241470112 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.33122029344708537 1101 2 -0.34686780600009115 1101 3 -0.35273774205202457 1101 4 -0.14959228925076928 1101 5 -0.1947127114413192 1101 6 -0.2937006296676401 1101 7 -0.27805096799458084 1101 8 -0.32279424982467475 1101 9 -0.49258107703843734 1101 10-11 -0.4489902057201576 1101 12-13 -0.46773052794976877 1101 14-15 -0.7258531792373546 1101 16-17 -0.6561390677975183 1101 18-19 -0.6781803767122057 1101 20-21 -0.7150393526973318 1101 22-23 -0.7688284953844899 1101 24-25 -0.6566853106967514 1101 26-27 -0.7994974995333948 1101 28-29 -0.810281554869384 1101 30-31 -0.7112728683270859 1101 32-33 -0.6634525766696342 1101 34-35 -0.6835590607996735 1101 36-37 -0.5924151685082606 1101 38-39 -0.6811181719466575 1101 40-41 -0.6843344243782639 1101 42-43 -0.7476432962620976 1101 44-45 -0.6693979033290205 1101 46-47 -0.7519959831118896 1101 48-49 -0.6460269495721676 1101 50-51 -0.7530509386965676 1101 52-53 -0.7632034581421436 1101 54-55 -0.6671079948405279 1101 56-57 -0.7350892107448104 1101 58-59 -0.7299783266287747 1101 60-61 -0.7310230051040847 1101 62-63 -0.8349069920880154 1101 64-65 -0.8175749204191192 1101 66-67 -0.7721044154559209 1101 68-69 -0.9079773275742937 1101 70-71 -1.009534122639188 1101 72-73 -1.070887287194843 1101 74-75 -0.9261338207481664 1101 76-77 -0.9267647852985341 1101 78-79 -0.7281295062028157 1101 80-81 -0.704661633447472 1101 82-83 -0.7285395374850836 1101 84-85 -0.6450025040992493 1101 86-87 -0.7887382092544435 1101 88-89 -0.8517749019926306 1101 90-91 -0.8451964085876611 1101 92-93 -0.919928241177292 1101 94-95 -0.9013381339634527 1101 96-97 -0.9351844113976391 1101 98-99 -1.1636238106767252 1101 100 -0.9527109593715579 1102 1 -0.41667186653020494 1102 2 -0.392570634560947 1102 3 -0.39975089157680443 1102 4 -0.22679662416303614 1102 5 -0.2866982784971839 1102 6 -0.350870384457167 1102 7 -0.32002736908998486 1102 8 -0.37929770446269373 1102 9 -0.5143200728245034 1102 10-11 -0.5885674224427433 1102 12-13 -0.572681191231581 1102 14-15 -0.6936535252974281 1102 16-17 -0.7681005583758989 1102 18-19 -0.7164089030115761 1102 20-21 -0.7382478372982888 1102 22-23 -0.7167065824711969 1102 24-25 -0.7406224433938817 1102 26-27 -0.7605261745219281 1102 28-29 -0.7181349710547451 1102 30-31 -0.761212399797202 1102 32-33 -0.7171081817214784 1102 34-35 -0.7416539397225606 1102 36-37 -0.8178714951333532 1102 38-39 -0.8016183116778635 1102 40-41 -0.7467872483399702 1102 42-43 -0.6958375696954064 1102 44-45 -0.7484574323363944 1102 46-47 -0.7632760078724701 1102 48-49 -0.8464009004209885 1102 50-51 -0.8996030445702061 1102 52-53 -0.9008070351131465 1102 54-55 -0.8730754233712261 1102 56-57 -0.8796175483538988 1102 58-59 -0.9003708044582837 1102 60-61 -0.9618364496590814 1102 62-63 -0.8708036758592357 1102 64-65 -0.9498105633495371 1102 66-67 -1.0496105707011267 1102 68-69 -1.0467775407903517 1102 70-71 -1.0235412170833413 1102 72-73 -1.058209927246601 1102 74-75 -1.110567135589342 1102 76-77 -0.957978208702027 1102 78-79 -0.835182285217563 1102 80-81 -0.8539357013968676 1102 82-83 -0.8917009137639837 1102 84-85 -0.8524341982802817 1102 86-87 -0.8159707954780302 1102 88-89 -0.8909459090474137 1102 90-91 -0.9766690997108789 1102 92-93 -1.1080927906442177 1102 94-95 -1.1282947106071042 1102 96-97 -1.178494207826418 1102 98-99 -1.2144026538465411 1102 100 -1.2157934843546911 1103 1 -0.32468675429454663 1103 2 -0.3478506851810472 1103 3 -0.37059242061205566 1103 4 -0.2469481315833164 1103 5 -0.21516735071217852 1103 6 -0.3591005619267662 1103 7 -0.33897659147711323 1103 8 -0.3558379625913375 1103 9 -0.5211552095336671 1103 10-11 -0.5212958620297172 1103 12-13 -0.5177681744701204 1103 14-15 -0.6801116485615282 1103 16-17 -0.6575485433005284 1103 18-19 -0.6516290583803155 1103 20-21 -0.6847840461380414 1103 22-23 -0.7658823677590547 1103 24-25 -0.8129117816215299 1103 26-27 -0.764175577300584 1103 28-29 -0.8111670066753689 1103 30-31 -0.7486904371484613 1103 32-33 -0.6720789387536428 1103 34-35 -0.8133407168082343 1103 36-37 -0.8130428710674522 1103 38-39 -0.7332206065172571 1103 40-41 -0.7518089938725296 1103 42-43 -0.702260739802739 1103 44-45 -0.7405914869402537 1103 46-47 -0.8195500183845681 1103 48-49 -0.7614145877179084 1103 50-51 -0.7607141199693928 1103 52-53 -0.8264165464113233 1103 54-55 -0.8222506744579476 1103 56-57 -0.7983538195511031 1103 58-59 -0.8646406067067787 1103 60-61 -0.9501185151262561 1103 62-63 -0.8320672446233601 1103 64-65 -0.877038393747732 1103 66-67 -0.9090472580476572 1103 68-69 -0.9084952669625679 1103 70-71 -1.0523234974057587 1103 72-73 -0.9421984985343244 1103 74-75 -0.9328062345281367 1103 76-77 -0.9003814297442112 1103 78-79 -0.7161712063294452 1103 80-81 -0.6481497445737077 1103 82-83 -0.7334165647532309 1103 84-85 -0.7422307328178057 1103 86-87 -0.7879521456964333 1103 88-89 -0.7953020288888091 1103 90-91 -0.8927768907705875 1103 92-93 -1.0643009547713724 1103 94-95 -0.9102044060561596 1103 96-97 -0.935235371685323 1103 98-99 -1.0120677582371975 1103 100 -1.1530089806916024 1104 1 -0.4132587062449318 1104 2 -0.4031144919457681 1104 3 -0.4334808993922721 1104 4 -0.2818985816870736 1104 5 -0.32768438247850185 1104 6 -0.3613409193868833 1104 7 -0.3911585870439822 1104 8 -0.4466317909854851 1104 9 -0.590849413599976 1104 10-11 -0.6303649100620774 1104 12-13 -0.6549194801999647 1104 14-15 -0.8235614367108326 1104 16-17 -0.6724098074029001 1104 18-19 -0.7468019882270198 1104 20-21 -0.7739550999581439 1104 22-23 -0.7926882575111946 1104 24-25 -0.7815852917892769 1104 26-27 -0.7715616236321239 1104 28-29 -0.8831645814006706 1104 30-31 -0.9021856855999175 1104 32-33 -0.8500395622393171 1104 34-35 -0.8529621527827729 1104 36-37 -0.7759025168923657 1104 38-39 -0.7346375201292759 1104 40-41 -0.8923130193719544 1104 42-43 -0.7379143893666011 1104 44-45 -0.8646650430128844 1104 46-47 -0.9122603902778721 1104 48-49 -0.9250931652818366 1104 50-51 -0.9234462039517553 1104 52-53 -0.7685298808265344 1104 54-55 -0.8065439306258639 1104 56-57 -0.8271721163826058 1104 58-59 -0.8139378526755721 1104 60-61 -0.8254545409059588 1104 62-63 -0.8611751344840428 1104 64-65 -0.929003306970678 1104 66-67 -0.9497536024927697 1104 68-69 -0.8897012083159481 1104 70-71 -0.8902500485787144 1104 72-73 -0.8785896562909841 1104 74-75 -0.9208832914149312 1104 76-77 -0.7376995100501595 1104 78-79 -0.697183439648704 1104 80-81 -0.7392779551577533 1104 82-83 -0.7984696673732365 1104 84-85 -0.7277957420306898 1104 86-87 -0.7566906285219162 1104 88-89 -0.9157283467469597 1104 90-91 -0.8132847392492124 1104 92-93 -0.8908808257623164 1104 94-95 -0.9352825349275626 1104 96-97 -0.9570875138749635 1104 98-99 -0.9264999136980521 1104 100 -1.0180866397211759 1105 1 -0.3627493154715218 1105 2 -0.3470070506004461 1105 3 -0.4240299420766185 1105 4 -0.19294007397513724 1105 5 -0.31862553625892787 1105 6 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68-69 -0.744849976238342 1105 70-71 -0.753998263112301 1105 72-73 -0.8105137957716337 1105 74-75 -0.7327907435971426 1105 76-77 -0.7454714437369674 1105 78-79 -0.7446718572464519 1105 80-81 -0.7222665919562807 1105 82-83 -0.6681317558949864 1105 84-85 -0.6490905700417571 1105 86-87 -0.7262866094747515 1105 88-89 -0.7460571895904238 1105 90-91 -0.7403371399176635 1105 92-93 -0.6809019867521826 1105 94-95 -0.7626114658049907 1105 96-97 -0.8469245045331313 1105 98-99 -0.8922199374078588 1105 100 -1.0426815856118665 1106 1 -0.4016323507306545 1106 2 -0.4296923825104706 1106 3 -0.48843400415766425 1106 4 -0.2937708592126853 1106 5 -0.3388995598780937 1106 6 -0.3800200914494454 1106 7 -0.44142958089157247 1106 8 -0.4323238390710884 1106 9 -0.5578862713794379 1106 10-11 -0.561969376773213 1106 12-13 -0.6423037682252186 1106 14-15 -0.7559339736754325 1106 16-17 -0.7150746601767182 1106 18-19 -0.7645872948228742 1106 20-21 -0.7876356019181117 1106 22-23 -0.7425550497550617 1106 24-25 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0.3738777898255563 2311 28-29 0.6700941578801931 2311 30-31 0.8790539435289872 2311 32-33 0.9938737836203941 2311 34-35 0.9749226808157445 2311 36-37 0.9792336317548731 2311 38-39 0.9731246048440241 2311 40-41 1.0286427541688994 2311 42-43 0.9919765720082339 2311 44-45 1.0515314884959892 2311 46-47 0.6881985528354662 2311 48-49 0.9157030048216654 2311 50-51 1.0088779250468463 2311 52-53 0.9352340743363925 2311 54-55 0.9669165841910186 2311 56-57 1.0743740223257632 2311 58-59 1.1257175273817737 2311 60-61 1.1776631462791656 2311 62-63 1.121690495453521 2311 64-65 1.1756636567866892 2311 66-67 1.1705639275550013 2311 68-69 1.1787428949119203 2311 70-71 1.1646269012935377 2311 72-73 1.1967518083002204 2311 74-75 1.17935118389628 2311 76-77 1.1150019828801412 2311 78-79 0.8610127993888241 2311 80-81 0.8028262554464263 2311 82-83 0.9113575580800024 2311 84-85 1.0048734827044434 2311 86-87 1.0410233798989132 2311 88-89 1.1120297217701314 2311 90-91 1.1193023796974728 2311 92-93 1.208213773475709 2311 94-95 1.1844518341714156 2311 96-97 1.1771372367638442 2311 98-99 1.213650532851986 2311 100 1.4184076806792092 2312 1 0.18658982668866742 2312 2 0.32312636735886713 2312 3 0.4317524602775791 2312 4 0.18101696047556715 2312 5 0.35304920685801733 2312 6 0.3917457192548852 2312 7 0.3004362056944885 2312 8 0.3897904610292571 2312 9 0.5682007189238263 2312 10-11 0.5981063054170122 2312 12-13 0.6669024186440424 2312 14-15 0.7968680592120805 2312 16-17 0.7788611525405358 2312 18-19 0.7712806357341293 2312 20-21 0.8235998118744519 2312 22-23 0.8879982823431192 2312 24-25 0.8916989635673929 2312 26-27 0.9399915825003475 2312 28-29 0.929575228147371 2312 30-31 0.9660598286731386 2312 32-33 0.8520305392502721 2312 34-35 0.9487166906125495 2312 36-37 1.0106284839587687 2312 38-39 0.7876864549665683 2312 40-41 0.9644271103364517 2312 42-43 1.0670340473312123 2312 44-45 0.8138373505677876 2312 46-47 0.8330587377335661 2312 48-49 1.0643473949737796 2312 50-51 0.9282072643309505 2312 52-53 1.071766323156048 2312 54-55 0.9374640182014176 2312 56-57 0.9000989998217435 2312 58-59 1.0714030448579948 2312 60-61 1.0676861170604681 2312 62-63 1.134368441617049 2312 64-65 1.0918258675538866 2312 66-67 1.1071957212639418 2312 68-69 1.1630827134964363 2312 70-71 1.1101898015227576 2312 72-73 1.1418317368484736 2312 74-75 1.1689853104769483 2312 76-77 1.1626087941110121 2312 78-79 1.0437881960326436 2312 80-81 1.0064794754914246 2312 82-83 1.030135942037898 2312 84-85 1.0663847753417492 2312 86-87 1.0578521738143856 2312 88-89 1.0590789026530594 2312 90-91 1.1122568458916362 2312 92-93 1.1236200864017043 2312 94-95 1.1826735213170174 2312 96-97 0.8683205004528354 2312 98-99 0.7027970926280105 2312 100 0.9911044716866968 2313 1 0.04866385971276799 2313 2 0.2392474836259808 2313 3 0.3188589779464408 2313 4 0.18316388520414506 2313 5 0.34497045262035186 2313 6 0.24410936219209134 2313 7 0.25648726824677937 2313 8 0.4278655731067005 2313 9 0.590306444057255 2313 10-11 0.3603666485678332 2313 12-13 0.4478219375829866 2313 14-15 0.4994001468542777 2313 16-17 0.7645946879934726 2313 18-19 0.7276437828437139 2313 20-21 0.6379055994554079 2313 22-23 0.6150272532794006 2313 24-25 0.557431607647608 2313 26-27 0.7613103472581244 2313 28-29 0.6082551222888384 2313 30-31 0.870255056397319 2313 32-33 0.9332626044771004 2313 34-35 0.9760210611827702 2313 36-37 1.0001624356452865 2313 38-39 0.9870308440814526 2313 40-41 1.0179109802501216 2313 42-43 1.0562347569119126 2313 44-45 0.9037666440614558 2313 46-47 0.9487904056883778 2313 48-49 1.0160830279817858 2313 50-51 1.0150454124283002 2313 52-53 1.040201667390157 2313 54-55 0.9469366537813784 2313 56-57 0.737879029315188 2313 58-59 0.8196972320520999 2313 60-61 0.6726327139382917 2313 62-63 1.0328769244651568 2313 64-65 1.0940243052486593 2313 66-67 1.0562746949032373 2313 68-69 1.1036272403319671 2313 70-71 1.0190916376746344 2313 72-73 1.1233214538634684 2313 74-75 1.1605206927689267 2313 76-77 1.1394417622526056 2313 78-79 1.0442429945315794 2313 80-81 0.9367229339019829 2313 82-83 0.9781557890064185 2313 84-85 1.0029249100294173 2313 86-87 1.0624061294578233 2313 88-89 1.0941231392596826 2313 90-91 0.9399550555013008 2313 92-93 1.111194101997615 2313 94-95 1.2101751806689478 2313 96-97 1.2465097207314635 2313 98-99 1.3010783452420895 2313 100 1.419336781104093 2314 1 -0.38354702810923413 2314 2 0.04940775592763913 2314 3 0.2768423112005678 2314 4 0.2101400750899174 2314 5 0.34154710729019655 2314 6 0.4029184616945898 2314 7 0.46074583775022404 2314 8 0.4631675131247306 2314 9 0.46258672011008883 2314 10-11 0.5705886985292992 2314 12-13 0.44538291049573075 2314 14-15 0.7759075224593843 2314 16-17 0.8213274153639887 2314 18-19 0.8665773825172352 2314 20-21 0.8696433698761865 2314 22-23 0.9408064651274586 2314 24-25 0.8515116783458296 2314 26-27 0.7870939680990361 2314 28-29 0.8087759528038063 2314 30-31 0.8806106489647831 2314 32-33 0.9146794877388729 2314 34-35 0.9481887761655159 2314 36-37 0.868811833144882 2314 38-39 0.6683149611340511 2314 40-41 0.6541079575820135 2314 42-43 0.9024819887631566 2314 44-45 0.8633267451511486 2314 46-47 0.7580260246665134 2314 48-49 0.8223752325012796 2314 50-51 0.7849848708863476 2314 52-53 0.8118537984182765 2314 54-55 0.7585852905532491 2314 56-57 0.7127131850723387 2314 58-59 0.8609641465038465 2314 60-61 0.898295014541354 2314 62-63 0.8707800902773073 2314 64-65 0.8657150561368496 2314 66-67 0.5260966798167672 2314 68-69 0.5336250678324959 2314 70-71 0.6989626066399879 2314 72-73 0.8548577728362687 2314 74-75 0.9821768375333377 2314 76-77 0.8190957753607258 2314 78-79 0.7472816446475932 2314 80-81 0.7497518160048955 2314 82-83 0.774403277303886 2314 84-85 0.846418956406847 2314 86-87 0.889917564959557 2314 88-89 0.8888199513602224 2314 90-91 0.9548884131272288 2314 92-93 0.8454027435389158 2314 94-95 0.8441803897740208 2314 96-97 0.9122202115794202 2314 98-99 1.0589366574335095 2314 100 1.0188271871685721 2315 1 0.41356829078347346 2315 2 0.42737750033899147 2315 3 0.44349330281834654 2315 4 0.27896140048693496 2315 5 0.34801451850307785 2315 6 0.39368204379304217 2315 7 0.3600329742027597 2315 8 0.2601359191334254 2315 9 0.5112831432755698 2315 10-11 0.5753030566949988 2315 12-13 0.42200046448994044 2315 14-15 0.6274039291404918 2315 16-17 0.776731604755696 2315 18-19 0.7508565027172054 2315 20-21 0.4947583076372837 2315 22-23 0.8300392321845322 2315 24-25 0.8882842208975958 2315 26-27 0.8028717769323279 2315 28-29 0.8027861712612037 2315 30-31 0.8261479532438329 2315 32-33 0.7452000393675675 2315 34-35 0.8373268242977332 2315 36-37 0.8584578586792588 2315 38-39 0.9142643040739102 2315 40-41 0.9286412812591465 2315 42-43 0.9216550678388202 2315 44-45 0.9308059933283559 2315 46-47 0.929977653837831 2315 48-49 0.8615101839973534 2315 50-51 0.9417679889796204 2315 52-53 0.9204919341237812 2315 54-55 0.7456517007351877 2315 56-57 0.7666964777958967 2315 58-59 0.7796984311917114 2315 60-61 0.9241709852286419 2315 62-63 0.9378875793202894 2315 64-65 1.0143614633377354 2315 66-67 1.0064410755251387 2315 68-69 1.0467087043815084 2315 70-71 0.9199696327131974 2315 72-73 1.0222820746180759 2315 74-75 0.4169635700045049 2315 76-77 0.4340816081631651 2315 78-79 0.7481117187219652 2315 80-81 0.7640880494315851 2315 82-83 0.7059105000012842 2315 84-85 0.7087328678197018 2315 86-87 0.8205715207895459 2315 88-89 0.8477673995029598 2315 90-91 0.870124533900551 2315 92-93 0.8453679572564639 2315 94-95 0.9095852289592976 2315 96-97 1.05388605321291 2315 98-99 1.0658808494453211 2315 100 1.071963521417917 2316 1 0.27860776144975574 2316 2 0.10324901090853444 2316 3 0.20499952318630932 2316 4 0.026977368759425246 2316 5 0.2660335352639578 2316 6 0.3530215728161039 2316 7 0.40858682357259113 2316 8 0.40258547536061684 2316 9 0.6321623273258723 2316 10-11 0.6170738626282315 2316 12-13 0.37758312933331695 2316 14-15 0.5367875538222151 2316 16-17 0.7737077597698843 2316 18-19 0.7343779945603259 2316 20-21 0.6798037649540944 2316 22-23 0.7895927825121731 2316 24-25 0.4347774372851134 2316 26-27 0.6990132790532115 2316 28-29 0.8972874008181861 2316 30-31 0.5820085647415709 2316 32-33 0.7158866286316581 2316 34-35 0.8400766072743906 2316 36-37 0.9052014459661919 2316 38-39 0.8396906888860514 2316 40-41 0.8280281228219479 2316 42-43 0.7831546744386557 2316 44-45 0.6714302424566299 2316 46-47 0.9136147153272987 2316 48-49 0.9801950835278816 2316 50-51 0.9923536239216872 2316 52-53 1.0325112005703758 2316 54-55 0.9100191916550457 2316 56-57 1.0172329652985468 2316 58-59 0.8322880436852884 2316 60-61 0.9869452664386316 2316 62-63 1.0050281959278777 2316 64-65 1.044403282791741 2316 66-67 1.0342517034266479 2316 68-69 1.0356913744508702 2316 70-71 0.962139999664096 2316 72-73 0.9730414559781693 2316 74-75 0.9790914265107133 2316 76-77 0.9182192430587754 2316 78-79 0.6377631458500304 2316 80-81 0.6403821501710567 2316 82-83 0.618063435405265 2316 84-85 0.6983228200019873 2316 86-87 0.6958909065626848 2316 88-89 0.7194794131601903 2316 90-91 0.5605430710325905 2316 92-93 0.43176987577182047 2316 94-95 0.4856980810960003 2316 96-97 0.6800172543463674 2316 98-99 0.8351821417140641 2316 100 0.515530276055248 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 1.0 11 1.0 12 41.0 13 174.0 14 633.0 15 2013.0 16 4834.0 17 10011.0 18 17208.0 19 25781.0 20 37931.0 21 54281.0 22 66601.0 23 66471.0 24 62745.0 25 64943.0 26 75400.0 27 91387.0 28 113854.0 29 139946.0 30 173412.0 31 218082.0 32 278965.0 33 371957.0 34 547838.0 35 762882.0 36 1198774.0 37 1718222.0 38 1290047.0 39 216996.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.905625025055684 21.602157187349462 13.74573396222819 22.746483825366667 2 15.330417635657259 20.12518519812866 41.44458547479105 23.099811691423035 3 18.77709653741084 27.090303376114093 29.03925050742678 25.093349579048287 4 12.352962281461586 16.756146030426795 39.55245467318577 31.338437014925844 5 12.840981664055029 38.82753900935736 34.24090543672205 14.090573889865562 6 29.530142970262013 38.23177812669687 18.01329918650117 14.22477971653995 7 27.19520454582779 32.3814685257263 22.859785518385312 17.5635414100606 8 26.68564948853982 35.27361368928336 19.5121608346801 18.52857598749672 9 26.813966756304968 15.391035591740243 21.74237305773826 36.05262459421653 10-11 26.157372037717618 26.317270285123517 26.625794219541394 20.89956345761747 12-13 26.04713574951604 24.015385259152293 27.652035425878264 22.285443565453402 14-15 23.526910110093592 25.105679837160753 25.625470262958366 25.74193978978728 16-17 21.7126170953226 27.734832114924046 26.91922946906014 23.633321320693216 18-19 21.144663370664407 27.594031775577903 29.62083536590894 21.64046948784876 20-21 22.785163442091523 26.684431149839938 29.030399233154508 21.50000617491403 22-23 23.037543603755555 26.424063085015547 28.735766660802685 21.80262665042621 24-25 22.304605994445303 26.41301763743355 29.62900434893912 21.653372019182022 26-27 22.237360301946442 26.730275943398706 28.603462220433094 22.428901534221755 28-29 21.44493873030429 27.297065071872172 29.131201364264008 22.12679483355953 30-31 22.620163292559027 26.677172610480255 29.084958088996455 21.617706007964266 32-33 22.898060443520542 26.90848980551874 28.19572168832935 21.99772806263136 34-35 22.72940108745048 26.850780315500995 29.033145766042285 21.386672831006244 36-37 22.325323895312618 26.52504816702458 28.728975389476062 22.420652548186737 38-39 22.57242303767904 26.613446305210015 29.176582489030583 21.637548168080357 40-41 22.213488172574195 25.661634916870696 29.83119912604031 22.293677784514806 42-43 22.25551423614601 25.933808802150978 28.987306598558614 22.823370363144402 44-45 22.14895500319391 26.91983068030287 28.833313284471924 22.09790103203129 46-47 22.232350665542537 26.624855191147685 28.111767466943633 23.031026676366146 48-49 23.078307179174143 26.571003556096752 28.104640899395804 22.246048365333298 50-51 22.657821718848076 26.404328070452294 28.00633774196689 22.93151246873274 52-53 22.057886559162743 27.006927860247092 27.765641886454045 23.16954369413612 54-55 22.05640170663736 26.88922223805532 27.772972004911285 23.281404050396034 56-57 23.063983503724337 26.387813566859776 27.309159991933168 23.239042937482715 58-59 21.960620021282995 26.99484060156323 28.140887434400536 22.903651942753235 60-61 22.281733469117952 27.5890009201068 27.360590287335306 22.76867532343994 62-63 22.69252786012216 26.81064681625162 27.00191438365478 23.49491093997144 64-65 21.791982295996497 26.881751874127353 27.442526290223086 23.883739539653064 66-67 22.27517537202427 26.563690336669854 27.445062850762124 23.716071440543754 68-69 21.862404035520086 26.677616006939008 27.608763468615333 23.851216488925576 70-71 21.863579445716734 26.228329455115425 27.86153855052011 24.046552548647732 72-73 22.453628748306286 26.491848395609342 27.856341014123508 23.198181841960867 74-75 22.56687270956809 26.913770510181745 27.37028796216642 23.149068818083745 76-77 22.296779285982154 26.704000907345193 27.06321970994524 23.936000096727415 78-79 22.183332454037522 26.737153997371838 27.22192740809722 23.857586140493424 80-81 21.72864439654238 27.245836258236533 27.67523372620646 23.350285619014624 82-83 22.77381986864761 26.236936506702502 28.641712135214668 22.34753148943522 84-85 22.956286706227473 26.717483549504234 27.26974514638324 23.056484597885056 86-87 21.562202515090206 27.44427552846532 27.699467996826872 23.294053959617607 88-89 22.448204297086512 26.240568294191192 27.629579070531978 23.681648338190314 90-91 22.30579180380296 26.185650878143313 27.836675714956648 23.671881603097074 92-93 22.637365049391885 26.493800820160864 27.554242618226954 23.3145915122203 94-95 21.46553159897706 26.478564729572806 28.172507075010717 23.883396596439418 96-97 21.7561480837592 26.47111107490693 27.710273030941966 24.06246781039191 98-99 21.100552251243993 27.013009301782514 27.626833514013516 24.259604932959974 100 21.777442908619886 26.471130940411953 26.91541084161241 24.836015309355755 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1698.0 1 1162.0 2 1034.0 3 1374.5 4 1006.5 5 795.0 6 1208.0 7 2368.0 8 3771.5 9 4333.5 10 3859.0 11 3404.5 12 3734.5 13 4497.0 14 5354.5 15 6528.5 16 7824.5 17 8947.5 18 10150.0 19 11079.5 20 11866.5 21 12781.5 22 13939.5 23 15187.5 24 16488.5 25 18543.0 26 21916.0 27 25452.0 28 30637.5 29 43090.0 30 61600.0 31 80030.0 32 107546.0 33 138028.0 34 161999.5 35 187322.5 36 209612.0 37 243910.0 38 257320.5 39 239977.5 40 219535.5 41 198772.5 42 193904.0 43 203623.0 44 222385.5 45 264580.0 46 304103.0 47 329473.5 48 352330.0 49 351877.0 50 337672.5 51 340197.5 52 340001.0 53 320368.5 54 302046.5 55 263234.5 56 226680.5 57 187933.5 58 149441.5 59 140738.5 60 113732.5 61 75797.5 62 55868.0 63 47192.0 64 34246.0 65 16887.0 66 9476.0 67 6187.5 68 4654.0 69 3818.5 70 3160.5 71 2516.5 72 2060.0 73 1939.5 74 1585.0 75 1119.5 76 708.0 77 379.0 78 238.0 79 155.0 80 96.0 81 66.5 82 44.0 83 31.5 84 20.5 85 10.5 86 8.0 87 5.0 88 5.0 89 4.0 90 1.0 91 0.5 92 1.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04451192763317022 2 0.0 3 0.0 4 0.03187306270309059 5 0.0 6 0.0 7 8.933916998393078E-4 8 0.0 9 0.011259363040621863 10-11 5.255245293172399E-4 12-13 1.9707169849396493E-5 14-15 1.3138113232930997E-4 16-17 2.1020981172689592E-4 18-19 0.0 20-21 1.3138113232930995E-5 22-23 0.0 24-25 6.109222653312913E-4 26-27 3.547290572891369E-4 28-29 1.7079547202810295E-4 30-31 0.0025750701936544753 32-33 0.015739459653051336 34-35 0.027392966090661126 36-37 0.03288469742202628 38-39 0.02314278645980795 40-41 0.0014189162291565476 42-43 1.0510490586344796E-4 44-45 0.0114235894560335 46-47 0.018537877771665635 48-49 0.003796914724317058 50-51 0.011114843795059623 52-53 0.011403882286184105 54-55 0.010569612095892987 56-57 3.678671705220679E-4 58-59 0.00882881209252963 60-61 0.004861101896184469 62-63 0.0015240211350199956 64-65 0.036372866485369464 66-67 0.06061925445674361 68-69 0.06033021596561914 70-71 0.05251303859202519 72-73 0.04629214197623236 74-75 0.03616265667364257 76-77 0.03194532232587172 78-79 0.015588371350872628 80-81 0.0010510490586344797 82-83 0.03913187026428497 84-85 0.052519607648641664 86-87 0.05832665369759715 88-89 0.0707158944762511 90-91 0.049189095944093646 92-93 0.04016978120968652 94-95 0.013538825686535392 96-97 0.00382319095078292 98-99 0.02328730570537019 100 0.03285842119556042 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7611443.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.12877551376081 #Duplication Level Percentage of deduplicated Percentage of total 1 77.7061442354562 50.60906023950946 2 14.142498762002786 18.421672541482394 3 4.296603039034496 8.394974844030607 4 1.68221955672144 4.382435994982757 5 0.7924236105618087 2.580478972204193 6 0.40585779493866414 1.5859812730242138 7 0.2363386975453321 1.0774714984351186 8 0.16003339301476632 0.8338223142691339 9 0.11052844016595498 0.6478723770709165 >10 0.41480235480859645 4.703556404070093 >50 0.029010742491894947 1.3252991666368998 >100 0.021072128150239462 2.6741980499991533 >500 0.0017550653042229048 0.7812137049377886 >1k 5.85004838701911E-4 0.660184850801808 >5k 5.086998594252932E-5 0.245517267707056 >10k+ 7.630497891379397E-5 1.0762605008385013 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27747 0.36454322787413634 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22881 0.3006131688826941 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14342 0.18842681998669633 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 9091 0.1194385874005757 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3138113232930997E-5 2 0.0 0.0 0.0 0.0 1.3138113232930997E-5 3 0.0 0.0 0.0 1.3138113232930997E-5 1.3138113232930997E-5 4 0.0 0.0 0.0 1.3138113232930997E-5 1.3138113232930997E-5 5 0.0 0.0 0.0 1.3138113232930997E-5 1.3138113232930997E-5 6 0.0 0.0 0.0 1.3138113232930997E-5 1.3138113232930997E-5 7 0.0 0.0 0.0 1.3138113232930997E-5 1.3138113232930997E-5 8 0.0 0.0 0.0 1.3138113232930997E-5 2.6276226465861994E-5 9 0.0 0.0 0.0 1.3138113232930997E-5 2.6276226465861994E-5 10-11 0.0 0.0 0.0 2.6276226465861994E-5 2.6276226465861994E-5 12-13 0.0 0.0 0.0 2.6276226465861994E-5 2.6276226465861994E-5 14-15 0.0 0.0 0.0 3.284528308232749E-5 2.6276226465861994E-5 16-17 0.0 0.0 0.0 5.912150954818948E-5 2.6276226465861994E-5 18-19 0.0 0.0 0.0 1.1167396247991346E-4 2.6276226465861994E-5 20-21 0.0 0.0 0.0 1.9050264187749945E-4 2.6276226465861994E-5 22-23 0.0 0.0 0.0 5.715079256324983E-4 3.284528308232749E-5 24-25 0.0 0.0 0.0 0.0013795018894577544 5.255245293172399E-5 26-27 0.0 0.0 0.0 0.0026341917032026646 5.255245293172399E-5 28-29 0.0 0.0 0.0 0.006720144918644205 5.255245293172399E-5 30-31 0.0 0.0 0.0 0.020685959285249855 5.255245293172399E-5 32-33 0.0 0.0 0.0 0.0535575185940432 5.255245293172399E-5 34-35 0.0 0.0 0.0 0.10055911868485384 5.255245293172399E-5 36-37 0.0 0.0 0.0 0.17692440185126523 5.255245293172399E-5 38-39 0.0 0.0 0.0 0.2899384518809377 5.255245293172399E-5 40-41 0.0 0.0 0.0 0.4125827389103486 5.255245293172399E-5 42-43 0.0 0.0 0.0 0.5340905791451109 5.255245293172399E-5 44-45 0.0 0.0 0.0 0.6690846926134768 7.882867939758599E-5 46-47 0.0 0.0 0.0 0.828509127638478 1.1167396247991346E-4 48-49 0.0 0.0 0.0 1.0087771793075242 1.3138113232930997E-4 50-51 0.0 0.0 0.0 1.1979200264654153 1.3138113232930997E-4 52-53 0.0 0.0 0.0 1.3787004645505458 1.4451924556224097E-4 54-55 0.0 0.0 0.0 1.5707731109593805 1.5765735879517194E-4 56-57 0.0 0.0 0.0 1.7919072638394584 1.5765735879517194E-4 58-59 0.0 0.0 0.0 2.0497427360357294 1.5765735879517194E-4 60-61 0.0 0.0 0.0 2.310146709369038 1.5765735879517194E-4 62-63 0.0 0.0 0.0 2.5788868155486417 1.5765735879517194E-4 64-65 0.0 0.0 0.0 2.8467663753114882 1.5765735879517194E-4 66-67 0.0 0.0 0.0 3.1429454309780684 1.5765735879517194E-4 68-69 0.0 0.0 0.0 3.4656766134884016 1.5765735879517194E-4 70-71 0.0 0.0 0.0 3.8173839047339646 1.5765735879517194E-4 72-73 0.0 0.0 0.0 4.169091195979528 1.8393358526103395E-4 74-75 0.0 0.0 0.0 4.503521868323785 1.8393358526103395E-4 76-77 0.0 0.0 0.0 4.8782996864063755 1.8393358526103395E-4 78-79 0.0 0.0 0.0 5.2820470441675775 1.8393358526103395E-4 80-81 0.0 0.0 0.0 5.709718906125947 1.8393358526103395E-4 82-83 0.0 0.0 0.0 6.147599082066305 1.9707169849396495E-4 84-85 0.0 0.0 0.0 6.567210711556271 1.9707169849396495E-4 86-87 0.0 0.0 0.0 7.003855904852733 1.9707169849396495E-4 88 0.0 0.0 0.0 7.354518716096278 1.9707169849396495E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17180 0.0 43.404655 1 TACCTGG 4410 0.0 39.431164 2 GTATCAA 29550 0.0 39.18891 1 ACCTGGG 4700 0.0 36.004143 3 GGGCACC 8110 0.0 34.54927 94 GTACTAG 7980 0.0 34.172867 1 CAACGCA 34405 0.0 32.09583 5 ATCAACG 34975 0.0 31.583332 3 TATCAAC 38230 0.0 30.11884 2 CCTGGGG 4570 0.0 29.72552 4 CTGTGCG 10530 0.0 29.675524 9 TAGGCAT 9620 0.0 29.6494 5 CTGGGGG 4065 0.0 29.014524 5 AACGCAG 39060 0.0 29.004623 6 TAGTACT 11260 0.0 28.052961 4 ACTGTGC 11770 0.0 27.58696 8 TCAACGC 40605 0.0 27.58623 4 GTGGTAT 5960 0.0 27.374079 1 GTACCTG 6365 0.0 27.257391 1 GCATCGT 9410 0.0 26.865496 8 >>END_MODULE