FastQCFastQC Report
Mon 6 Jun 2016
ERR1211245_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211245_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9110092
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279740.3070660537786007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218530.23987683110115685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129870.14255618933376304No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC120670.13245749878266871No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA212900.037.4260861
GTACTAG101550.036.4743771
GTATCAA351750.036.3403241
GGGCACC108100.035.52104694
TATTCTC42000.032.9042365
CAACGCA373800.032.2805065
ATCAACG385300.031.3416063
CTGTGCG141200.031.126539
GCATCGT112250.030.5693048
TAGGCAT131300.030.5378255
TAGTACT147350.030.4014424
TACCCCG4950.030.387755
TACCTGG38750.030.3623352
ACCTGGG38000.030.3067023
ACTGTGC149600.030.1325598
TATCAAC426050.029.9126532
GTACTGT154500.028.8428656
CTAACGC56750.028.5765233
AACGCAG435650.028.571886
TCTAACG57950.028.3425452
Produced by FastQC (version 0.11.5)