FastQCFastQC Report
Mon 6 Jun 2016
ERR1211245_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211245_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9110092
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246340.2704034163431061No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211210.2318417860105035No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC122630.134608958943554No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121520.1333905299748894No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA105610.11592638142402951No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC107600.038.01146394
GGTATCA218100.037.2260251
GTACTAG111550.035.5699231
GTATCAA352050.035.4410481
TAGGCAT132750.032.597355
TAGTACT148600.031.0915344
CTGTGCG144650.031.0523389
CAACGCA383700.030.6864415
ACTGTGC151350.030.2054468
ATCAACG392600.030.049063
ACCTGGG37100.030.0249213
TACCTGG39600.029.1932832
CTAGGCA152350.029.123064
GTACTGT161250.028.4385416
TATCAAC440150.028.2188642
TACTGTG175750.028.0705687
AACGCAG445050.027.44876
GCATCGT136550.027.2169598
CTAACGC56550.027.1783183
TCTAACG56950.027.0659432
Produced by FastQC (version 0.11.5)