FastQCFastQC Report
Mon 6 Jun 2016
ERR1211244_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211244_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7572274
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222510.2938483208610782No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175630.23193825263058362No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102790.1357452199960012No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147100.044.1729931
GTATCAA255600.040.056581
GGGCACC78150.039.27022694
GTACTAG91000.038.0550421
GCATCGT89750.036.2416738
GTGGTAT40450.035.9431231
CAACGCA280600.033.8412325
ATCAACG287250.033.1400533
TAGGCAT112150.032.4851535
TATCAAC317650.032.0397572
CTGTGCG114850.030.571769
TAGTACT119800.029.8221724
AACGCAG330900.029.7201026
CTAGGCA127150.029.4292534
TATTCTC37400.028.9093785
ACTGTGC126250.028.8912898
CATCGTC118000.027.9631429
ACGCAGA342800.027.6046717
CGCAGAG342100.027.5622358
TACTGTG142150.027.5472187
Produced by FastQC (version 0.11.5)