FastQCFastQC Report
Mon 6 Jun 2016
ERR1211244_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211244_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7572274
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203570.2688360194044748No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170170.2247277370047624No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102910.13590369286689835No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA154200.043.5181851
GGGCACC81450.039.42214294
GTATCAA262050.038.3308331
GTACTAG86650.035.547081
GTGGTAT39200.035.148981
TACCCCG5450.034.4852335
CAACGCA292800.032.1745875
ATCAACG297850.031.6582263
TAGGCAT106500.031.2799685
CTGTGCG116050.031.2157089
TATCAAC333350.030.1177482
TAGTACT123650.029.918094
ACCTGGG35100.029.7302883
TACCTGG36800.029.6320612
ACTGTGC129400.029.2660888
AACGCAG341850.028.6163866
GTACTGT133450.028.0614036
CTAGGCA124300.027.9086284
TGAACCG11150.027.8124185
AAGCTAA52300.027.670249
Produced by FastQC (version 0.11.5)