##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211244_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7572274 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1974260308066 33.0 31.0 34.0 30.0 34.0 2 32.344798669461774 34.0 31.0 34.0 31.0 34.0 3 32.425143358520835 34.0 31.0 34.0 31.0 34.0 4 36.021856314232686 37.0 35.0 37.0 35.0 37.0 5 35.91554729794511 37.0 35.0 37.0 35.0 37.0 6 35.93977053656537 37.0 35.0 37.0 35.0 37.0 7 35.88069369914506 37.0 35.0 37.0 35.0 37.0 8 35.87415682528128 37.0 35.0 37.0 35.0 37.0 9 37.47818581313883 39.0 37.0 39.0 35.0 39.0 10-11 37.461921610337924 39.0 37.0 39.0 35.0 39.0 12-13 37.34825159522754 39.0 37.0 39.0 34.0 39.0 14-15 38.569410391118964 40.0 38.0 41.0 34.0 41.0 16-17 38.52976939291948 40.0 38.0 41.0 34.0 41.0 18-19 38.55320891716279 40.0 38.0 41.0 34.0 41.0 20-21 38.511920065227436 40.0 38.0 41.0 34.0 41.0 22-23 38.36889612024076 40.0 38.0 41.0 34.0 41.0 24-25 38.29907066226077 40.0 38.0 41.0 34.0 41.0 26-27 38.1544136543395 40.0 38.0 41.0 33.5 41.0 28-29 38.143540962727975 40.0 37.5 41.0 33.5 41.0 30-31 38.16398680237931 40.0 37.5 41.0 34.0 41.0 32-33 38.04220931519382 40.0 37.0 41.0 33.0 41.0 34-35 37.9077667421966 40.0 37.0 41.0 33.0 41.0 36-37 37.77728295357511 40.0 37.0 41.0 33.0 41.0 38-39 37.68036140794694 40.0 37.0 41.0 33.0 41.0 40-41 37.57092651428091 40.0 36.0 41.0 32.5 41.0 42-43 37.41533633093573 40.0 36.0 41.0 32.0 41.0 44-45 37.23346520477204 39.0 36.0 41.0 31.5 41.0 46-47 36.96602889699977 39.0 35.0 41.0 31.0 41.0 48-49 36.68831522736763 39.0 35.0 41.0 31.0 41.0 50-51 36.455761373663975 39.0 35.0 40.5 30.0 41.0 52-53 36.21718046652828 38.5 35.0 40.0 30.0 41.0 54-55 35.94902000376637 38.0 35.0 40.0 29.5 41.0 56-57 35.64689722004249 37.5 34.5 40.0 29.0 41.0 58-59 35.33182924970755 37.0 34.0 40.0 28.0 41.0 60-61 35.05865761064642 36.5 34.0 40.0 28.0 41.0 62-63 34.731373891119105 36.0 34.0 39.0 27.5 41.0 64-65 34.351683589368264 35.5 33.0 39.0 27.0 40.5 66-67 34.055366129117886 35.0 33.0 38.5 26.0 40.0 68-69 33.74909498784645 35.0 33.0 38.0 26.0 40.0 70-71 33.48654519104829 35.0 33.0 37.0 26.0 40.0 72-73 33.19417779916574 35.0 32.5 37.0 26.0 39.5 74-75 32.89151660386298 35.0 32.0 36.0 25.5 39.0 76-77 31.9309197606954 34.0 30.5 35.0 24.5 38.0 78-79 32.4489429463329 35.0 32.0 35.0 25.0 38.0 80-81 32.41225799541855 35.0 32.0 35.0 25.0 37.0 82-83 32.19619707369279 35.0 32.0 35.0 25.0 37.0 84-85 31.97873102584508 34.0 32.0 35.0 25.0 36.5 86-87 31.772988668925606 34.0 31.0 35.0 24.0 36.0 88-89 31.585139880041318 34.0 31.0 35.0 24.0 36.0 90-91 31.386728940342095 34.0 31.0 35.0 24.0 35.0 92-93 31.21206469813427 34.0 31.0 35.0 23.0 35.0 94-95 31.047330035865052 34.0 31.0 35.0 23.0 35.0 96-97 30.89228037707035 34.0 30.5 35.0 21.5 35.0 98-99 30.72859909718005 34.0 30.0 35.0 20.0 35.0 100 28.85350015068129 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3405921341605307 1101 2 -0.34512452805914506 1101 3 -0.3746798058851013 1101 4 -0.20000848200830035 1101 5 -0.27406355280452743 1101 6 -0.33251226209484486 1101 7 -0.27363951299429345 1101 8 -0.3001505436871028 1101 9 -0.4531471376745344 1101 10-11 -0.41525552427099655 1101 12-13 -0.45374855619594 1101 14-15 -0.7218697930536209 1101 16-17 -0.6656401037664708 1101 18-19 -0.7183131376945298 1101 20-21 -0.6945726914267425 1101 22-23 -0.7470344376108997 1101 24-25 -0.6886631184531353 1101 26-27 -0.8079818073404113 1101 28-29 -0.7998302095736491 1101 30-31 -0.6420880722907398 1101 32-33 -0.6636644766745761 1101 34-35 -0.747656543117067 1101 36-37 -0.6816802268320004 1101 38-39 -0.7013543582701871 1101 40-41 -0.6918702089249535 1101 42-43 -0.7333435208858816 1101 44-45 -0.7892189006663273 1101 46-47 -0.8213031784303482 1101 48-49 -0.763382838495076 1101 50-51 -0.8385800027477828 1101 52-53 -0.8005896339649112 1101 54-55 -0.7529348397598739 1101 56-57 -0.8061805258540247 1101 58-59 -0.7494496145486735 1101 60-61 -0.7437290663989273 1101 62-63 -0.9086346160544849 1101 64-65 -0.7500293239070359 1101 66-67 -0.8249058165209888 1101 68-69 -0.9134823747155068 1101 70-71 -0.8945169711455208 1101 72-73 -1.00805211301482 1101 74-75 -1.0664041582464847 1101 76-77 -0.999242669138841 1101 78-79 -0.854014725185646 1101 80-81 -0.8123338555208548 1101 82-83 -0.8561684108320406 1101 84-85 -0.8086325451313243 1101 86-87 -0.9301073160074047 1101 88-89 -0.9325712117651612 1101 90-91 -0.9362679042122615 1101 92-93 -1.103388520546531 1101 94-95 -1.0454994090042717 1101 96-97 -1.2183018287396372 1101 98-99 -1.2316606582714869 1101 100 -1.1352306274810893 1102 1 -0.40166431368754374 1102 2 -0.34443239303190154 1102 3 -0.3784144106889258 1102 4 -0.19511093504088706 1102 5 -0.31733345883057495 1102 6 -0.3453381769698822 1102 7 -0.3641164497240652 1102 8 -0.3529358502332087 1102 9 -0.4932921826751411 1102 10-11 -0.5420168874833493 1102 12-13 -0.553410479056005 1102 14-15 -0.6912623238318858 1102 16-17 -0.7050737506901257 1102 18-19 -0.7173261414968408 1102 20-21 -0.8071575724626925 1102 22-23 -0.783953634668741 1102 24-25 -0.8642193943759651 1102 26-27 -0.8634771367940743 1102 28-29 -0.8985593154593232 1102 30-31 -0.8368006775997117 1102 32-33 -0.722049415263065 1102 34-35 -0.7108952727313707 1102 36-37 -0.7770809810820225 1102 38-39 -0.8905904633773929 1102 40-41 -0.7478863384376879 1102 42-43 -0.683458471239085 1102 44-45 -0.8363803067609652 1102 46-47 -0.9117701044413664 1102 48-49 -0.8185549734106843 1102 50-51 -0.7544495886856524 1102 52-53 -0.8202780482203522 1102 54-55 -0.8370315814043963 1102 56-57 -0.8119298939804906 1102 58-59 -0.8334308082304105 1102 60-61 -0.7472399164709387 1102 62-63 -0.7611003743910061 1102 64-65 -0.7992249687226121 1102 66-67 -0.8819632731322571 1102 68-69 -0.8612808750855621 1102 70-71 -0.9237818816408918 1102 72-73 -0.9827257833016674 1102 74-75 -1.139234624661647 1102 76-77 -0.9450403908569989 1102 78-79 -0.8196248727296869 1102 80-81 -0.6861302713559212 1102 82-83 -0.7012516887878988 1102 84-85 -0.8756093802674663 1102 86-87 -0.822431145407041 1102 88-89 -0.924567702795887 1102 90-91 -0.8742986126077277 1102 92-93 -0.965742138811617 1102 94-95 -1.0245109360738915 1102 96-97 -1.1611625875897325 1102 98-99 -1.1706240016282798 1102 100 -1.1798789791434423 1103 1 -0.3466434407456518 1103 2 -0.3237588829409006 1103 3 -0.4095312322094067 1103 4 -0.25089943304035245 1103 5 -0.24339946745288898 1103 6 -0.3787058295787844 1103 7 -0.45941186800506983 1103 8 -0.42706183854443225 1103 9 -0.6431447113396374 1103 10-11 -0.5898635448578418 1103 12-13 -0.6724915147175707 1103 14-15 -0.813411159798342 1103 16-17 -0.8214885596519608 1103 18-19 -0.7505713101767952 1103 20-21 -0.7860058129001573 1103 22-23 -0.8106547080563189 1103 24-25 -0.8742148808663543 1103 26-27 -0.8169354198538912 1103 28-29 -0.9259726956569168 1103 30-31 -0.8800517093489972 1103 32-33 -0.8017856571333439 1103 34-35 -0.9007164995891586 1103 36-37 -0.8873869771267167 1103 38-39 -0.877970518436122 1103 40-41 -0.875966537746983 1103 42-43 -0.7423807867491021 1103 44-45 -0.8217660639412401 1103 46-47 -0.9176672250736928 1103 48-49 -0.9097455983992973 1103 50-51 -0.9692925454094095 1103 52-53 -0.9858546300829616 1103 54-55 -0.9794537944885136 1103 56-57 -1.0230411105364539 1103 58-59 -1.0167527303492179 1103 60-61 -0.9840415074765261 1103 62-63 -0.966522989179694 1103 64-65 -1.0605417009803375 1103 66-67 -1.054406087654577 1103 68-69 -1.1094990099565436 1103 70-71 -1.170336167338256 1103 72-73 -1.0200344706682571 1103 74-75 -1.0350607032047705 1103 76-77 -1.0187170934485898 1103 78-79 -0.8247075628813505 1103 80-81 -0.7994550560000988 1103 82-83 -0.8329433103209851 1103 84-85 -0.9107221115218671 1103 86-87 -0.9732816894047325 1103 88-89 -0.991601803115568 1103 90-91 -0.9235083091842711 1103 92-93 -1.0624198282259378 1103 94-95 -1.1036518544688256 1103 96-97 -1.2151224402229737 1103 98-99 -1.1421921047983155 1103 100 -1.3348430710423749 1104 1 -0.3463002091197964 1104 2 -0.3441057237401566 1104 3 -0.4234435514280577 1104 4 -0.2607362715621946 1104 5 -0.2851199667308606 1104 6 -0.33456124059548387 1104 7 -0.36648639715411235 1104 8 -0.3080463175495467 1104 9 -0.46145757830436906 1104 10-11 -0.45682462065308016 1104 12-13 -0.493438979675652 1104 14-15 -0.6496626125067309 1104 16-17 -0.5663224045799566 1104 18-19 -0.6360913901926821 1104 20-21 -0.7013947604876876 1104 22-23 -0.618054483325011 1104 24-25 -0.7124957642530916 1104 26-27 -0.7040654732927152 1104 28-29 -0.7982006033075493 1104 30-31 -0.8084538919874547 1104 32-33 -0.7698197547879815 1104 34-35 -0.7806418980675076 1104 36-37 -0.7813542995202454 1104 38-39 -0.8018629445461087 1104 40-41 -0.7825332615505189 1104 42-43 -0.7011818953194506 1104 44-45 -0.7588128788316268 1104 46-47 -0.8948077855168748 1104 48-49 -0.8456309608470818 1104 50-51 -0.7479303092059766 1104 52-53 -0.810989726103216 1104 54-55 -0.8328468289448097 1104 56-57 -0.826897374893889 1104 58-59 -0.8894767232901373 1104 60-61 -0.8481842168245279 1104 62-63 -0.9163796534073043 1104 64-65 -0.8523853714996292 1104 66-67 -0.8198018892108792 1104 68-69 -0.9094797387653202 1104 70-71 -1.0008004277994473 1104 72-73 -0.9677303289375558 1104 74-75 -0.9658399343655262 1104 76-77 -0.8711067201554776 1104 78-79 -0.7412453374442691 1104 80-81 -0.7302990241182528 1104 82-83 -0.7027739888220381 1104 84-85 -0.7533819395245764 1104 86-87 -0.7835127282114769 1104 88-89 -0.8477851664277622 1104 90-91 -0.8261513094009061 1104 92-93 -0.7282848967693489 1104 94-95 -0.8728773274263766 1104 96-97 -0.8674443260166775 1104 98-99 -0.98925963933792 1104 100 -1.1763037009825652 1105 1 -0.40723462769230423 1105 2 -0.3542406716891229 1105 3 -0.40641476155658296 1105 4 -0.2603314303109414 1105 5 -0.2994046373378154 1105 6 -0.3445423864060402 1105 7 -0.3597587565817406 1105 8 -0.4265359801510229 1105 9 -0.5463007918693705 1105 10-11 -0.5246484984959636 1105 12-13 -0.5489556914670004 1105 14-15 -0.8204992212652158 1105 16-17 -0.7329966019541203 1105 18-19 -0.7073800091199658 1105 20-21 -0.7780138125174432 1105 22-23 -0.7292949797335524 1105 24-25 -0.7892604820625095 1105 26-27 -0.798704313705656 1105 28-29 -0.7605576447390234 1105 30-31 -0.7753026826420211 1105 32-33 -0.6948740372790425 1105 34-35 -0.7692942632850404 1105 36-37 -0.7508626412247068 1105 38-39 -0.7772908915586996 1105 40-41 -0.8267680783413809 1105 42-43 -0.8035793278320398 1105 44-45 -0.8066959091007391 1105 46-47 -0.8186912809261386 1105 48-49 -0.8680261035986447 1105 50-51 -0.8307481784228017 1105 52-53 -0.8983629978233125 1105 54-55 -0.8710145077778293 1105 56-57 -0.8973870133758055 1105 58-59 -0.9097436142261373 1105 60-61 -0.8705040658121206 1105 62-63 -0.8932690102494192 1105 64-65 -0.818505034594871 1105 66-67 -0.8982414290471823 1105 68-69 -0.9454629400497083 1105 70-71 -1.0052407133055823 1105 72-73 -0.9705951458428288 1105 74-75 -1.0868571193734873 1105 76-77 -1.0573182915590955 1105 78-79 -0.9642372636406442 1105 80-81 -0.8796319055958222 1105 82-83 -0.8793628334968346 1105 84-85 -0.8385676189182192 1105 86-87 -0.9310790614484965 1105 88-89 -0.8699699718267233 1105 90-91 -0.8669715909332929 1105 92-93 -1.069656030753535 1105 94-95 -0.9702626531369951 1105 96-97 -1.1028042063485124 1105 98-99 -1.1779579814714651 1105 100 -1.126863271817296 1106 1 -0.44161720584705577 1106 2 -0.3716873676635082 1106 3 -0.4397490906205306 1106 4 -0.26026945305954285 1106 5 -0.3280205592857186 1106 6 -0.3231686897429711 1106 7 -0.3930217559712261 1106 8 -0.356634973056579 1106 9 -0.46071573140206823 1106 10-11 -0.5282218719041936 1106 12-13 -0.5983957904136474 1106 14-15 -0.6686506281009699 1106 16-17 -0.6372286970293004 1106 18-19 -0.6335183206490527 1106 20-21 -0.6638757246488467 1106 22-23 -0.6175244009330214 1106 24-25 -0.6543259055521844 1106 26-27 -0.6981409089833619 1106 28-29 -0.6300051769676003 1106 30-31 -0.7095391256965726 1106 32-33 -0.6750992958725419 1106 34-35 -0.6616364274859876 1106 36-37 -0.6645022768495181 1106 38-39 -0.6709818586255736 1106 40-41 -0.6923383133237024 1106 42-43 -0.5903850809162776 1106 44-45 -0.6627939641317511 1106 46-47 -0.7283001617956515 1106 48-49 -0.8112954319263679 1106 50-51 -0.8098281041904869 1106 52-53 -0.7870240961623054 1106 54-55 -0.8020331390010611 1106 56-57 -0.8106463005113866 1106 58-59 -0.740135298091424 1106 60-61 -0.7629593763851474 1106 62-63 -0.8503100806675405 1106 64-65 -0.8706765994319383 1106 66-67 -0.8075309615600332 1106 68-69 -0.8127834341479883 1106 70-71 -0.8660983739730312 1106 72-73 -0.8832798571955536 1106 74-75 -0.9065878485561889 1106 76-77 -0.9394260932108303 1106 78-79 -0.7085758371492545 1106 80-81 -0.6878771014814262 1106 82-83 -0.7649704354972755 1106 84-85 -0.7325739859840361 1106 86-87 -0.7090576725965256 1106 88-89 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0.3915055834690122 2310 6 0.42232417882214435 2310 7 0.4564293724115984 2310 8 0.45019826350524283 2310 9 0.6470987074737451 2310 10-11 0.6386538989067034 2310 12-13 0.6865623019224572 2310 14-15 0.8953585776348305 2310 16-17 0.7072352039507521 2310 18-19 0.7432759127015984 2310 20-21 0.8805318735511349 2310 22-23 0.9537059211219514 2310 24-25 0.9509407226194213 2310 26-27 0.9614354223614185 2310 28-29 0.9424754799525346 2310 30-31 0.8178509895022685 2310 32-33 0.9573565856017296 2310 34-35 1.0099554753992592 2310 36-37 0.9206504115310068 2310 38-39 0.9733459570102525 2310 40-41 1.0703198267073972 2310 42-43 1.0870653443314282 2310 44-45 1.0570985201277878 2310 46-47 0.6595818753899678 2310 48-49 0.9197134888553506 2310 50-51 1.00124446973512 2310 52-53 1.0477321553404195 2310 54-55 1.0570916784001838 2310 56-57 1.1104261936610769 2310 58-59 1.03292451539604 2310 60-61 1.0962782475934105 2310 62-63 0.4941039500813531 2310 64-65 0.6913808604878611 2310 66-67 1.0926665222674004 2310 68-69 1.185803129264066 2310 70-71 1.166176891137546 2310 72-73 1.157222329086622 2310 74-75 1.1827098380403527 2310 76-77 1.1480410298617407 2310 78-79 0.9857738877219973 2310 80-81 0.9534264029110702 2310 82-83 0.988066606832426 2310 84-85 1.0543013115376958 2310 86-87 1.0509043044369228 2310 88-89 1.0817295636132975 2310 90-91 1.1488142701497246 2310 92-93 1.1894097497488865 2310 94-95 1.2261551478149464 2310 96-97 1.260752295417749 2310 98-99 1.2702782152691263 2310 100 1.5038336767789993 2311 1 0.380100989333819 2311 2 0.3365602974337989 2311 3 0.39735069363043607 2311 4 0.3199184808223947 2311 5 0.4033925579439739 2311 6 0.4597104635556022 2311 7 0.4907091713863494 2311 8 0.4951436266634559 2311 9 0.6240484366149204 2311 10-11 0.6720049856716059 2311 12-13 0.6819354355935232 2311 14-15 0.7810744962174354 2311 16-17 0.6594781950040698 2311 18-19 0.7751460203044687 2311 20-21 0.6778448576529854 2311 22-23 0.8578773392003143 2311 24-25 0.8964944888936017 2311 26-27 0.31266199720667487 2311 28-29 0.6369373167694548 2311 30-31 0.8216111649743283 2311 32-33 0.9372076262593154 2311 34-35 1.0037449994155665 2311 36-37 1.0337738228802422 2311 38-39 1.0698886686038236 2311 40-41 1.0434664091540284 2311 42-43 1.0291959923281269 2311 44-45 1.0873244513388656 2311 46-47 0.8096554669447613 2311 48-49 1.0765822586119427 2311 50-51 1.1505163677433288 2311 52-53 1.0572350076554002 2311 54-55 1.0881386201903211 2311 56-57 1.2084069465569343 2311 58-59 1.1909028368146437 2311 60-61 1.1969061145379527 2311 62-63 1.1682513416414082 2311 64-65 1.256607479638852 2311 66-67 1.2383802829473112 2311 68-69 1.2892178067657127 2311 70-71 1.3069834316245093 2311 72-73 1.3424238407661804 2311 74-75 1.3101393502222294 2311 76-77 1.3178625071189565 2311 78-79 1.0179978139652306 2311 80-81 0.9685517460970132 2311 82-83 1.0893419179834467 2311 84-85 1.12626441046746 2311 86-87 1.114114412229295 2311 88-89 1.1549097223319116 2311 90-91 1.1790530526922502 2311 92-93 1.2286632942408495 2311 94-95 1.3440387897514974 2311 96-97 1.3021477551744631 2311 98-99 1.355970439626418 2311 100 1.4918953370135526 2312 1 0.25176374405743474 2312 2 0.3334602805822868 2312 3 0.4350403575625208 2312 4 0.17578284108633824 2312 5 0.3453144407810669 2312 6 0.4075878014716281 2312 7 0.3302348561793593 2312 8 0.4132381161386718 2312 9 0.5774398470019904 2312 10-11 0.6149746408986161 2312 12-13 0.7115051315659784 2312 14-15 0.8354433143226103 2312 16-17 0.8385999689748189 2312 18-19 0.8128989005831286 2312 20-21 0.8466136724752147 2312 22-23 0.9636512599124245 2312 24-25 0.9861755504781513 2312 26-27 0.9922083816962939 2312 28-29 0.9705236699282267 2312 30-31 0.9118841277446563 2312 32-33 0.8142072477952027 2312 34-35 0.9396092325981726 2312 36-37 0.9981453785292302 2312 38-39 0.7922471340807888 2312 40-41 0.9144388143682249 2312 42-43 0.9881146929691056 2312 44-45 0.7109003163160068 2312 46-47 0.7485602535030509 2312 48-49 0.864106511621884 2312 50-51 0.7427049956315628 2312 52-53 0.8821418709345394 2312 54-55 0.8378362725082269 2312 56-57 0.8644136908694122 2312 58-59 0.936982185607512 2312 60-61 0.9836253006561151 2312 62-63 0.9935156542763224 2312 64-65 0.9649829737502884 2312 66-67 0.9874140213215199 2312 68-69 1.0465654312779478 2312 70-71 0.9197015465529006 2312 72-73 0.94741134642031 2312 74-75 1.01146873353224 2312 76-77 0.9877120031024553 2312 78-79 0.8897141215587254 2312 80-81 0.8775680544715172 2312 82-83 0.9386277407232484 2312 84-85 0.952091183778311 2312 86-87 0.9716532147286223 2312 88-89 0.9672310059275482 2312 90-91 1.0358983220298903 2312 92-93 1.1080978537723034 2312 94-95 1.088382809786932 2312 96-97 0.7805423789984793 2312 98-99 0.5890337074674115 2312 100 0.9319374485917606 2313 1 0.02989502305318581 2313 2 0.23125327085323022 2313 3 0.2448630124692457 2313 4 0.1592869023079757 2313 5 0.29163791083338 2313 6 0.23558055203839245 2313 7 0.18902826700357167 2313 8 0.3434547854228427 2313 9 0.5915711485601918 2313 10-11 0.34639200639625045 2313 12-13 0.4220591760270338 2313 14-15 0.5016225327159347 2313 16-17 0.7647973742956964 2313 18-19 0.6887638881701221 2313 20-21 0.6309414661147343 2313 22-23 0.602546842656352 2313 24-25 0.5169246678732833 2313 26-27 0.7006897516594535 2313 28-29 0.5376336266254143 2313 30-31 0.7884146048020853 2313 32-33 0.8665712560678074 2313 34-35 0.9084147894982166 2313 36-37 0.9647522080207978 2313 38-39 0.9218765655061603 2313 40-41 0.9224455287270672 2313 42-43 0.9518852066974475 2313 44-45 0.8226993158820903 2313 46-47 0.8214212963489729 2313 48-49 0.9516673757888867 2313 50-51 0.8939945391992481 2313 52-53 0.9520464426945594 2313 54-55 0.8390788968454075 2313 56-57 0.6036310146573527 2313 58-59 0.6014589219939026 2313 60-61 0.5062742866712355 2313 62-63 0.8761504495899999 2313 64-65 0.981610227012716 2313 66-67 0.9889155099356657 2313 68-69 1.077643387380597 2313 70-71 0.9734939017859716 2313 72-73 1.008145933520872 2313 74-75 1.070875586007702 2313 76-77 1.0613912623870014 2313 78-79 0.8959066595065224 2313 80-81 0.7733924389005082 2313 82-83 0.9187047316130403 2313 84-85 0.8853169784297741 2313 86-87 0.9220300108476884 2313 88-89 0.9264889141142909 2313 90-91 0.8755685086142826 2313 92-93 1.0259631430742395 2313 94-95 1.1276388829115724 2313 96-97 1.1701068850480603 2313 98-99 1.1868276446930999 2313 100 1.2744724238255536 2314 1 -0.40289423520341217 2314 2 0.001239402734313444 2314 3 0.24166495244412545 2314 4 0.16462942254138824 2314 5 0.2666011373780961 2314 6 0.3750552779798184 2314 7 0.40345391738771497 2314 8 0.4192663580358982 2314 9 0.36161541043045276 2314 10-11 0.5261705802859851 2314 12-13 0.4112568901163556 2314 14-15 0.6811499875472506 2314 16-17 0.7728120278430808 2314 18-19 0.7706060791707472 2314 20-21 0.8273247591466912 2314 22-23 0.8379261399625904 2314 24-25 0.7416002757140063 2314 26-27 0.6578274419693599 2314 28-29 0.7260285368733292 2314 30-31 0.7761981821620338 2314 32-33 0.7726083947440827 2314 34-35 0.8263566318660978 2314 36-37 0.7812397740285917 2314 38-39 0.5557238656588126 2314 40-41 0.5880782508314581 2314 42-43 0.6935876933711498 2314 44-45 0.7814901307749764 2314 46-47 0.6676674437714709 2314 48-49 0.6888173674523443 2314 50-51 0.702540638064491 2314 52-53 0.7367695060621671 2314 54-55 0.6825890178628171 2314 56-57 0.6496246471532316 2314 58-59 0.74107756955555 2314 60-61 0.8127303130433958 2314 62-63 0.7485500837353101 2314 64-65 0.7453185570726006 2314 66-67 0.4907060944791155 2314 68-69 0.5000526503968743 2314 70-71 0.648177806248114 2314 72-73 0.8131365976699669 2314 74-75 0.888457081071607 2314 76-77 0.7241219258639937 2314 78-79 0.6404043192255884 2314 80-81 0.7376417221490144 2314 82-83 0.7283374309145145 2314 84-85 0.7735470053334801 2314 86-87 0.8366365577664077 2314 88-89 0.8523951824756004 2314 90-91 0.9017938968945423 2314 92-93 0.7713109476480007 2314 94-95 0.8419465206856955 2314 96-97 0.8926361303578894 2314 98-99 1.0045264912319531 2314 100 1.0436235795358115 2315 1 0.38370189132752586 2315 2 0.39145403871525986 2315 3 0.41602524106077254 2315 4 0.28259543507270024 2315 5 0.3151297773826798 2315 6 0.36886053090786675 2315 7 0.3451449198382974 2315 8 0.2487961720126748 2315 9 0.4077761348665305 2315 10-11 0.5431012611612616 2315 12-13 0.35394173863270595 2315 14-15 0.555980316444753 2315 16-17 0.6966946930989479 2315 18-19 0.7243745540367925 2315 20-21 0.45079985188770877 2315 22-23 0.772507246424226 2315 24-25 0.8243927664105541 2315 26-27 0.8170358838250067 2315 28-29 0.8023997704932029 2315 30-31 0.8559679114570216 2315 32-33 0.7807103398898931 2315 34-35 0.7877751261797883 2315 36-37 0.7709798718605185 2315 38-39 0.837879718370182 2315 40-41 0.8201307557883766 2315 42-43 0.9006341031384579 2315 44-45 0.8924424161409164 2315 46-47 0.8531620949892087 2315 48-49 0.7629744362420183 2315 50-51 0.8188592022325523 2315 52-53 0.8615207398795093 2315 54-55 0.6809795159876018 2315 56-57 0.6668929665897565 2315 58-59 0.731185051319656 2315 60-61 0.8047603443280096 2315 62-63 0.8542772813281658 2315 64-65 0.9329344499248009 2315 66-67 0.9405268040071846 2315 68-69 0.9644122493486904 2315 70-71 0.8287829626380088 2315 72-73 0.9576860059124215 2315 74-75 0.3388292426674582 2315 76-77 0.34638514966480827 2315 78-79 0.6523365964274817 2315 80-81 0.7121928288123556 2315 82-83 0.6648502952775956 2315 84-85 0.7608794441999684 2315 86-87 0.7579988997338596 2315 88-89 0.795469245191132 2315 90-91 0.8631555865032432 2315 92-93 0.8923075123312394 2315 94-95 0.9375148968995752 2315 96-97 1.0176463739821777 2315 98-99 1.0014822726240702 2315 100 0.9831267552396525 2316 1 0.2773593827281218 2316 2 0.04417340384770796 2316 3 0.1831621728463162 2316 4 -0.03321175558566125 2316 5 0.2359526693848295 2316 6 0.3246772533724922 2316 7 0.3878141317158281 2316 8 0.3995608838455311 2316 9 0.6118626210979912 2316 10-11 0.6002316224591979 2316 12-13 0.3801033236681377 2316 14-15 0.5264846268374299 2316 16-17 0.7534105229470569 2316 18-19 0.7340885434708468 2316 20-21 0.701510898474254 2316 22-23 0.7258613094827524 2316 24-25 0.43014982977746286 2316 26-27 0.6865521310568354 2316 28-29 0.8873521917013178 2316 30-31 0.551467018647628 2316 32-33 0.7092091202128401 2316 34-35 0.8078503433277078 2316 36-37 0.8637609022011006 2316 38-39 0.759855963134747 2316 40-41 0.7968066065981603 2316 42-43 0.7553845938664949 2316 44-45 0.6401195576316425 2316 46-47 0.8564828345582711 2316 48-49 0.8821997257550578 2316 50-51 0.8958548289711956 2316 52-53 0.9069927449214603 2316 54-55 0.7882627798414674 2316 56-57 0.9446625741183539 2316 58-59 0.7933537281396212 2316 60-61 0.9252599392608616 2316 62-63 0.9333029234151695 2316 64-65 0.9611186136303402 2316 66-67 0.9131042043734396 2316 68-69 0.9527138599019978 2316 70-71 0.8961391459967487 2316 72-73 0.9405220783047952 2316 74-75 0.9270391947703018 2316 76-77 0.8671084643595854 2316 78-79 0.633448119821928 2316 80-81 0.6470953432948363 2316 82-83 0.5649309192553247 2316 84-85 0.6995761420403461 2316 86-87 0.715613412895518 2316 88-89 0.7233943689311495 2316 90-91 0.5573998114187582 2316 92-93 0.4492637020497874 2316 94-95 0.5196843189946314 2316 96-97 0.6577732977420432 2316 98-99 0.8459215833084066 2316 100 0.41048294527471185 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 3.0 12 28.0 13 153.0 14 572.0 15 1702.0 16 4048.0 17 8231.0 18 14429.0 19 22109.0 20 32714.0 21 48183.0 22 59289.0 23 59074.0 24 57723.0 25 62500.0 26 73738.0 27 91128.0 28 113281.0 29 138984.0 30 171744.0 31 216195.0 32 277304.0 33 369993.0 34 548751.0 35 767251.0 36 1227003.0 37 1730992.0 38 1257959.0 39 217177.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.719977148498295 21.599531132000187 13.538193558138417 23.1422981613631 2 15.256579463447837 19.838307488609104 41.25710453689341 23.648008511049653 3 18.949485980037174 26.480763902626876 28.74362443831272 25.82612567902324 4 12.421782516457922 16.432861336800812 39.449010199664215 31.69634594707705 5 13.01354124269671 38.73436169900878 34.352045369726454 13.900051688568057 6 30.09394535908236 38.13083097626948 17.52612227185651 14.24910139279165 7 27.37310160851536 31.993911941047514 22.782110879268906 17.85087557116822 8 26.77965694321151 35.364277626509555 19.163397943603204 18.69266748667573 9 26.399068812933287 15.213386249934127 21.721988147255512 36.66555678987708 10-11 26.4716561837527 26.13617432352437 26.23715497753803 21.1550145151849 12-13 26.200463599269575 23.619217348556855 27.46857696946326 22.71174208271031 14-15 23.48608180367733 24.83809497648887 25.248751578705626 26.42707164112818 16-17 21.894378511874237 27.638590953700525 26.536614283998432 23.930416250426806 18-19 21.266656489186737 27.693101174099088 29.11257569390648 21.927666642807694 20-21 23.091400488902046 26.753933280407793 28.419106890420693 21.73555934026947 22-23 23.37520406683646 26.517014571844598 28.05737748000138 22.050403881317553 24-25 22.658135747631754 26.317141715564148 29.104752034096208 21.91997050270789 26-27 22.607521569089926 26.73940519422498 27.85208174625819 22.800991490426902 28-29 21.722915934691372 27.26824080230193 28.533761017101693 22.475082245905003 30-31 22.771914312815735 26.725545268124897 28.560101240379538 21.942439178679827 32-33 23.070089886803437 26.974401795886926 27.57924300872346 22.376265308586174 34-35 22.95763417903995 26.80050545046106 28.48451252180072 21.757347848698274 36-37 22.49901467944337 26.424708794594466 28.08068802753125 22.99558849843092 38-39 22.79387089916115 26.48142843716752 28.721202762365877 22.00349790130545 40-41 22.417838546550232 25.487258331964284 29.389220835681844 22.705682285803636 42-43 22.459085747014154 25.76062566183097 28.597153072800698 23.183135518354174 44-45 22.591058526248943 26.69122229426985 28.305115559837468 22.412603619643736 46-47 22.524912072609812 26.52713638544541 27.626738115892113 23.321213426052662 48-49 23.500838284129124 26.42441939166787 27.710266025621976 22.364476298581025 50-51 22.996423985809265 26.234595274320128 27.622485799721673 23.14649494014893 52-53 22.34817727140411 26.78642749632961 27.387360270153614 23.478034962112666 54-55 22.315269420031946 26.743521681212908 27.314261691826413 23.626947206928733 56-57 23.43340950841748 26.18047994595 26.917374674114303 23.468735871518216 58-59 22.112742278552567 26.891524320140842 27.8158725924028 23.179860808903793 60-61 22.390520786476937 27.47196408190606 27.00297310025735 23.134542031359658 62-63 22.909329486260464 26.649409600742928 26.52840059988136 23.912860313115246 64-65 21.961137537742804 26.8506812777797 27.005427512647977 24.18275367182952 66-67 22.547234785246935 26.49589263311513 27.089183365659647 23.86768921597828 68-69 22.088311170727625 26.48720001659701 27.30055934310716 24.1239294695682 70-71 21.90647477023808 26.076698803925286 27.50551005128092 24.511316374555715 72-73 22.63565215495629 26.19216075648244 27.614799870255386 23.55738721830588 74-75 22.793635498383548 26.568724699919738 27.12353556531649 23.514104236380224 76-77 22.439610350022214 26.463061635749703 26.687657479811772 24.409670534416307 78-79 22.364368069779054 26.484940029166182 26.797926377115495 24.352765523939265 80-81 21.81509768926422 27.02320864937706 27.36965360852598 23.792040052832743 82-83 22.88170228091062 25.864674438300238 28.45979594226901 22.79382733852013 84-85 23.066421659338424 26.255597592508344 27.10277953476179 23.575201213391438 86-87 21.562007264366446 27.081148490058983 27.52080948629136 23.83603475928321 88-89 22.59762022363885 25.731174145247966 27.460128090975317 24.21107754013787 90-91 22.25125570376556 25.826055877909194 27.52429984151344 24.39838857681181 92-93 22.596149654058095 26.078166884435454 27.348131041762723 23.977552419743724 94-95 21.365383321262392 26.026920780014883 28.13769177983268 24.47000411889004 96-97 21.72115913958287 25.98089785912625 27.632956168589228 24.66498683270165 98-99 21.023342883276072 26.46870574752711 27.54606451062383 24.961886858572985 100 21.731330593774153 25.887288632995904 26.77371529166239 25.607665481567555 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 636.0 1 432.0 2 311.5 3 434.5 4 391.5 5 351.0 6 574.0 7 1138.0 8 1833.5 9 2198.0 10 2054.0 11 1976.0 12 2336.0 13 2988.5 14 3724.0 15 4635.5 16 5601.5 17 6534.0 18 7542.0 19 8406.5 20 9233.5 21 9899.5 22 10726.5 23 11880.5 24 13260.0 25 15014.5 26 17690.0 27 21038.0 28 26157.0 29 37278.5 30 53886.5 31 70606.0 32 95019.5 33 123533.5 34 145344.0 35 167932.0 36 188964.0 37 223525.0 38 240075.5 39 227030.0 40 210610.0 41 193025.0 42 190481.5 43 203386.5 44 226783.0 45 273245.5 46 317753.0 47 346045.5 48 370132.5 49 369590.0 50 353820.0 51 362149.5 52 364754.0 53 341181.5 54 320293.5 55 276149.0 56 236448.5 57 193436.0 58 149470.0 59 143116.5 60 117332.5 61 76157.5 62 54152.0 63 45492.5 64 32481.5 65 14170.0 66 7232.0 67 4755.5 68 3607.5 69 2841.5 70 2196.0 71 1770.0 72 1499.0 73 1415.5 74 1138.5 75 814.5 76 551.5 77 325.5 78 201.0 79 143.5 80 94.5 81 54.0 82 39.0 83 25.5 84 13.5 85 6.5 86 3.0 87 5.0 88 5.0 89 1.5 90 0.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04497988318964686 2 0.0 3 0.0 4 0.03321327252553196 5 0.0 6 0.0 7 9.772493705325507E-4 8 0.0 9 0.011396840632021505 10-11 4.1599128610507225E-4 12-13 1.98091088621463E-5 14-15 1.3866376203502408E-4 16-17 2.311062700583735E-4 18-19 0.0 20-21 6.603036287382099E-6 22-23 0.0 24-25 7.065248827498846E-4 26-27 4.952277215536574E-4 28-29 1.6507590718455248E-4 30-31 0.0025949932609411652 32-33 0.015794462799417982 34-35 0.026372526931804103 36-37 0.031727589360870986 38-39 0.0229059328809285 40-41 0.0014526679832240618 42-43 5.9427326586438894E-5 44-45 0.011799625845551812 46-47 0.018442280350658206 48-49 0.003420372796863928 50-51 0.011297795087710772 52-53 0.011594931720642966 54-55 0.010406385188914189 56-57 3.3675485065648707E-4 58-59 0.00883486255251725 60-61 0.005018307578410396 62-63 0.0014526679832240618 64-65 0.03677230908443091 66-67 0.06143464961780305 68-69 0.06136861925492924 70-71 0.05361005161725527 72-73 0.047000412293585786 74-75 0.03641574512491228 76-77 0.03241430513475872 78-79 0.015543547420497463 80-81 9.97058479394697E-4 82-83 0.039545584325131396 84-85 0.053682685016416466 86-87 0.05957919642104869 88-89 0.07113450992396736 90-91 0.050301930437276834 92-93 0.04072752782057279 94-95 0.013991833892962668 96-97 0.003902394445842821 98-99 0.022747460010031334 100 0.03226243530014894 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7572274.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.37716986462392 #Duplication Level Percentage of deduplicated Percentage of total 1 79.60261695405987 56.81809511998524 2 13.3795074979398 19.09982758770917 3 3.9217523745188907 8.397707562090813 4 1.4764127734248054 4.215286612761713 5 0.6538071929578735 2.3333453535233546 6 0.32364498986161955 1.3860518050312383 7 0.1960196975695255 0.9793931875162561 8 0.11099807940873738 0.633818301488357 9 0.07244922077761883 0.4654098304203366 >10 0.24143814788266307 2.851139246881217 >50 0.013355218894230463 0.662657111648215 >100 0.007310496815504162 0.9520074072919571 >500 4.296050250747242E-4 0.22056389502319784 >1k 1.9331314812891614E-4 0.25393186902721715 >5k 0.0 0.0 >10k+ 6.443771581010748E-5 0.7307651096017339 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20357 0.2688360194044748 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17017 0.2247277370047624 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10291 0.13590369286689835 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 7.923643544858519E-5 0.0 4 0.0 0.0 0.0 7.923643544858519E-5 0.0 5 0.0 0.0 0.0 1.0564858059811359E-4 0.0 6 0.0 0.0 0.0 1.0564858059811359E-4 0.0 7 0.0 0.0 0.0 1.0564858059811359E-4 0.0 8 1.3206072574764199E-5 0.0 0.0 1.0564858059811359E-4 0.0 9 1.3206072574764199E-5 0.0 0.0 1.1885465317287779E-4 0.0 10-11 1.3206072574764199E-5 0.0 0.0 1.3866376203502408E-4 0.0 12-13 1.3206072574764199E-5 0.0 0.0 1.6507590718455248E-4 0.0 14-15 1.3206072574764199E-5 0.0 0.0 1.8488501604669878E-4 0.0 16-17 1.3206072574764199E-5 0.0 0.0 2.1129716119622718E-4 0.0 18-19 1.3206072574764199E-5 0.0 0.0 2.443123426331377E-4 0.0 20-21 1.3206072574764199E-5 0.0 0.0 3.96182177242926E-4 0.0 22-23 1.3206072574764199E-5 0.0 0.0 6.074793384391532E-4 0.0 24-25 1.3206072574764199E-5 0.0 0.0 0.0011621343865792495 0.0 26-27 1.3206072574764199E-5 0.0 0.0 0.0023044596642963528 6.603036287382099E-6 28-29 1.3206072574764199E-5 0.0 0.0 0.0055267413725388174 1.3206072574764199E-5 30-31 1.3206072574764199E-5 0.0 0.0 0.019809108862146298 1.3206072574764199E-5 32-33 1.3206072574764199E-5 0.0 0.0 0.05456088884263829 1.3206072574764199E-5 34-35 1.3206072574764199E-5 0.0 0.0 0.10346957862327749 1.3206072574764199E-5 36-37 1.3206072574764199E-5 0.0 0.0 0.18460768852262874 1.9809108862146297E-5 38-39 1.3206072574764199E-5 0.0 0.0 0.30832877943930714 2.6412145149528397E-5 40-41 1.3206072574764199E-5 0.0 0.0 0.43764924512768555 2.6412145149528397E-5 42-43 1.3206072574764199E-5 0.0 0.0 0.5619976244916652 3.3015181436910494E-5 44-45 1.3206072574764199E-5 0.0 0.0 0.7073304531769453 4.6221254011674694E-5 46-47 1.3206072574764199E-5 0.0 0.0 0.8773718436496091 5.2824290299056794E-5 48-49 1.3206072574764199E-5 0.0 0.0 1.073145266534201 5.2824290299056794E-5 50-51 1.3206072574764199E-5 0.0 0.0 1.2732502812233155 5.2824290299056794E-5 52-53 1.3206072574764199E-5 0.0 0.0 1.459409683273479 6.603036287382099E-5 54-55 1.3206072574764199E-5 0.0 0.0 1.6585308455557737 7.923643544858519E-5 56-57 1.3206072574764199E-5 1.3206072574764199E-5 0.0 1.891175623069107 7.923643544858519E-5 58-59 1.3206072574764199E-5 1.3206072574764199E-5 0.0 2.16221045355728 7.923643544858519E-5 60-61 1.3206072574764199E-5 2.6412145149528397E-5 0.0 2.4349950886616094 7.923643544858519E-5 62-63 1.3206072574764199E-5 2.6412145149528397E-5 0.0 2.7143959661258954 7.923643544858519E-5 64-65 1.3206072574764199E-5 2.6412145149528397E-5 0.0 2.9951174508476583 7.923643544858519E-5 66-67 1.3206072574764199E-5 2.6412145149528397E-5 0.0 3.302898178275113 7.923643544858519E-5 68-69 1.3206072574764199E-5 2.6412145149528397E-5 0.0 3.641178330313985 7.923643544858519E-5 70-71 1.3206072574764199E-5 2.6412145149528397E-5 0.0 4.007230852977586 7.923643544858519E-5 72-73 1.3206072574764199E-5 2.6412145149528397E-5 0.0 4.378209240711575 7.923643544858519E-5 74-75 1.3206072574764199E-5 2.6412145149528397E-5 0.0 4.72765512711241 7.923643544858519E-5 76-77 1.3206072574764199E-5 2.6412145149528397E-5 0.0 5.123427916105518 7.923643544858519E-5 78-79 1.3206072574764199E-5 2.6412145149528397E-5 0.0 5.545705292756178 7.923643544858519E-5 80-81 1.3206072574764199E-5 2.6412145149528397E-5 0.0 5.991595127170517 7.923643544858519E-5 82-83 1.3206072574764199E-5 2.6412145149528397E-5 0.0 6.446280205919649 9.244250802334939E-5 84-85 1.3206072574764199E-5 2.6412145149528397E-5 0.0 6.877999924461265 9.244250802334939E-5 86-87 1.3206072574764199E-5 2.6412145149528397E-5 0.0 7.33345755845602 9.244250802334939E-5 88 1.3206072574764199E-5 2.6412145149528397E-5 0.0 7.700474124417579 9.244250802334939E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15420 0.0 43.518185 1 GGGCACC 8145 0.0 39.422142 94 GTATCAA 26205 0.0 38.330833 1 GTACTAG 8665 0.0 35.54708 1 GTGGTAT 3920 0.0 35.14898 1 TACCCCG 545 0.0 34.485233 5 CAACGCA 29280 0.0 32.174587 5 ATCAACG 29785 0.0 31.658226 3 TAGGCAT 10650 0.0 31.279968 5 CTGTGCG 11605 0.0 31.215708 9 TATCAAC 33335 0.0 30.117748 2 TAGTACT 12365 0.0 29.91809 4 ACCTGGG 3510 0.0 29.730288 3 TACCTGG 3680 0.0 29.632061 2 ACTGTGC 12940 0.0 29.266088 8 AACGCAG 34185 0.0 28.616386 6 GTACTGT 13345 0.0 28.061403 6 CTAGGCA 12430 0.0 27.908628 4 TGAACCG 1115 0.0 27.812418 5 AAGCTAA 5230 0.0 27.67024 9 >>END_MODULE