FastQCFastQC Report
Mon 6 Jun 2016
ERR1211243_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211243_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7095136
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191200.26948038769094773No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149820.21115874311641103No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90910.1281300316160254No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126000.040.8191831
GTATCAA212700.037.7642361
GTACTAG68250.037.506881
GCATCGT63400.037.441848
TACCTGG41200.033.8064772
CAACGCA231900.032.3710065
TAGGCAT84800.031.873085
ATCAACG236550.031.7344473
GGGCACC70000.030.07781294
TATCAAC267750.029.5598352
TCTAACG30900.029.5426032
ACCTGGG46350.029.5115623
CTAGGCA95000.029.2421844
CTAACGC30750.029.1969323
CTGTGCG82000.029.0064649
TAACGCC31500.028.9492364
AACGCAG271850.028.755516
CATCGTC88550.027.6571439
GTACATG310100.027.5871471
CGCCTAC33300.027.5263447
Produced by FastQC (version 0.11.5)