##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211243_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7095136 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.518868278212004 31.0 31.0 34.0 30.0 34.0 2 31.537926404793367 31.0 31.0 34.0 28.0 34.0 3 31.607286315582957 33.0 31.0 34.0 28.0 34.0 4 35.20344698114314 37.0 35.0 37.0 32.0 37.0 5 35.27016550493183 37.0 35.0 37.0 33.0 37.0 6 35.32641178407292 37.0 35.0 37.0 32.0 37.0 7 35.24836986916107 37.0 35.0 37.0 32.0 37.0 8 35.25538382914718 37.0 35.0 37.0 32.0 37.0 9 36.83220843124078 39.0 37.0 39.0 32.0 39.0 10-11 36.783636916896306 39.0 37.0 39.0 32.0 39.0 12-13 36.68053882265259 39.0 37.0 39.0 32.0 39.0 14-15 37.88737587271054 40.0 38.0 41.0 32.0 41.0 16-17 37.86834727339969 40.0 38.0 41.0 32.0 41.0 18-19 37.88536921631946 40.0 38.0 41.0 32.0 41.0 20-21 37.85770885857579 40.0 38.0 41.0 32.0 41.0 22-23 37.748001870577255 40.0 37.0 41.0 32.0 41.0 24-25 37.65683723328206 40.0 37.0 41.0 32.0 41.0 26-27 37.51310552750504 40.0 37.0 41.0 31.5 41.0 28-29 37.4017463513032 40.0 37.0 41.0 31.0 41.0 30-31 37.26328191594918 40.0 36.0 41.0 31.0 41.0 32-33 37.096440998453026 39.5 36.0 41.0 31.0 41.0 34-35 36.962695922953415 39.0 36.0 41.0 30.0 41.0 36-37 36.86222554437293 39.0 35.5 41.0 30.0 41.0 38-39 36.728874048362144 39.0 35.0 41.0 30.0 41.0 40-41 36.570462426653975 39.0 35.0 41.0 30.0 41.0 42-43 36.366249427777 39.0 35.0 40.0 29.0 41.0 44-45 36.14010506634404 39.0 35.0 40.0 28.5 41.0 46-47 35.916504067011545 38.0 35.0 40.0 28.0 41.0 48-49 35.71574392654348 38.0 34.5 40.0 27.0 41.0 50-51 35.02903270634982 37.5 33.5 39.5 26.5 40.5 52-53 35.12789938064612 38.0 34.0 40.0 26.5 40.5 54-55 35.60450786285139 38.0 34.0 40.0 27.5 41.0 56-57 35.557712424398915 38.0 34.0 40.0 27.0 41.0 58-59 35.341022074841135 38.0 34.0 40.0 27.0 41.0 60-61 35.12685591932276 37.0 34.0 40.0 26.5 41.0 62-63 34.83259721871434 37.0 34.0 40.0 26.0 41.0 64-65 34.59049417798334 36.0 34.0 40.0 26.0 41.0 66-67 34.4352664980629 36.0 33.5 39.5 26.0 41.0 68-69 34.15359014964618 35.5 33.0 39.0 26.0 41.0 70-71 33.881733133797574 35.0 33.0 39.0 26.0 41.0 72-73 33.56660640472572 35.0 33.0 38.0 25.0 40.0 74-75 33.241791348326515 35.0 33.0 37.0 25.0 40.0 76-77 32.92546844768022 35.0 32.0 37.0 24.5 39.0 78-79 32.59357480955968 35.0 32.0 36.0 24.0 39.0 80-81 32.29994386295062 35.0 32.0 36.0 23.5 38.5 82-83 32.004369119915395 35.0 31.5 35.5 23.0 37.0 84-85 31.758349029532347 35.0 31.0 35.0 23.0 37.0 86-87 31.537468978748258 35.0 31.0 35.0 22.0 36.5 88-89 31.335938874180847 34.0 31.0 35.0 20.5 36.0 90-91 31.104182710521687 34.0 31.0 35.0 20.0 36.0 92-93 30.905420840417996 34.0 30.5 35.0 20.0 36.0 94-95 30.747375525994144 34.0 30.0 35.0 19.5 35.0 96-97 30.568908827117617 34.0 30.0 35.0 19.0 35.0 98-99 30.340239637407937 34.0 29.5 35.0 18.0 35.0 100 29.18463620711428 33.0 27.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.47830224577924696 1101 2 -0.329239613309376 1101 3 -0.3974667894453887 1101 4 -0.34913091201293867 1101 5 -0.2830818425900503 1101 6 -0.24609481128497634 1101 7 -0.3391139736981259 1101 8 -0.35815822733286495 1101 9 -0.5155561994254043 1101 10-11 -0.4606177886124172 1101 12-13 -0.5475107098854295 1101 14-15 -0.6419750139717664 1101 16-17 -0.5994712479123407 1101 18-19 -0.5703229439835695 1101 20-21 -0.6724003845796389 1101 22-23 -0.7875110296526131 1101 24-25 -0.8054679305275698 1101 26-27 -0.7855007061931261 1101 28-29 -0.7136501640601978 1101 30-31 -0.8006605218035858 1101 32-33 -0.843331954253209 1101 34-35 -0.8456306510409703 1101 36-37 -0.8605491221556676 1101 38-39 -0.9261338916554394 1101 40-41 -1.0004200137155124 1101 42-43 -1.1015310665555234 1101 44-45 -1.0509762397745348 1101 46-47 -1.2344441874108796 1101 48-49 -1.274162222390693 1101 50-51 -1.3255971755893015 1101 52-53 -1.1079071074598374 1101 54-55 -0.9840521989339663 1101 56-57 -1.0031848549375084 1101 58-59 -0.9207603527242796 1101 60-61 -0.7552345376986622 1101 62-63 -0.7897804974983842 1101 64-65 -0.8976934840772302 1101 66-67 -0.8925160612066705 1101 68-69 -0.9770036429039592 1101 70-71 -0.9664069016598731 1101 72-73 -1.0140527938074726 1101 74-75 -0.9703870716182905 1101 76-77 -1.0866641691086372 1101 78-79 -1.076056733325231 1101 80-81 -1.1445763578830963 1101 82-83 -1.040047402254654 1101 84-85 -1.171946326484715 1101 86-87 -1.2039284086041668 1101 88-89 -1.1783518095321206 1101 90-91 -1.254119014683031 1101 92-93 -1.3715963740629782 1101 94-95 -1.3820809330977752 1101 96-97 -1.4562748581520886 1101 98-99 -1.5477816964403317 1101 100 -1.5059091571501533 1102 1 -0.3999038371793091 1102 2 -0.4021602997950673 1102 3 -0.41883668334945057 1102 4 -0.23666095589668856 1102 5 -0.30511936752754565 1102 6 -0.27063125062770865 1102 7 -0.29020783223324287 1102 8 -0.2469002101083504 1102 9 -0.3520706469960544 1102 10-11 -0.43079611558031417 1102 12-13 -0.5188671605401822 1102 14-15 -0.68068740600674 1102 16-17 -0.6898922286975022 1102 18-19 -0.6742240589693012 1102 20-21 -0.7109974687149858 1102 22-23 -0.7395525178880362 1102 24-25 -0.727181784509817 1102 26-27 -0.7339022117590872 1102 28-29 -0.813018774740577 1102 30-31 -0.8387334215382864 1102 32-33 -0.8820751649006837 1102 34-35 -0.8790367845149447 1102 36-37 -0.9461614005247725 1102 38-39 -1.0823638656479417 1102 40-41 -1.0739665007542527 1102 42-43 -0.9921418753762481 1102 44-45 -1.1340690661175685 1102 46-47 -1.1026140032768552 1102 48-49 -1.0329881857634646 1102 50-51 -1.114322893933398 1102 52-53 -0.9866165233669761 1102 54-55 -0.8116484221946152 1102 56-57 -0.7978307922014309 1102 58-59 -0.8606682486818116 1102 60-61 -0.8787180914252772 1102 62-63 -0.8741213436542097 1102 64-65 -0.9413742506123768 1102 66-67 -0.9645099966884274 1102 68-69 -1.0544572478581742 1102 70-71 -0.9676597696180238 1102 72-73 -1.0043245637830083 1102 74-75 -1.0278966977449144 1102 76-77 -1.1751317614738461 1102 78-79 -1.0439053193624197 1102 80-81 -1.1283587756526998 1102 82-83 -1.1723364548208295 1102 84-85 -1.1036539865349049 1102 86-87 -1.1725846615520332 1102 88-89 -1.2854394959640238 1102 90-91 -1.2653160378535588 1102 92-93 -1.2099995762361964 1102 94-95 -1.193881825663265 1102 96-97 -1.210945887857239 1102 98-99 -1.3305693868481931 1102 100 -1.281340335119868 1103 1 -0.5724412966248487 1103 2 -0.4830839567632914 1103 3 -0.5907685583453919 1103 4 -0.44257611780071215 1103 5 -0.4805611649749153 1103 6 -0.533497539399292 1103 7 -0.5201020172806281 1103 8 -0.5095126209101792 1103 9 -0.6610957599208902 1103 10-11 -0.7587689010739851 1103 12-13 -0.7236513426224178 1103 14-15 -0.8334403478961718 1103 16-17 -0.9143231676131265 1103 18-19 -0.8028679004265129 1103 20-21 -0.8574804039165826 1103 22-23 -0.856685493238281 1103 24-25 -0.8807939723838416 1103 26-27 -0.9504775429583745 1103 28-29 -0.9742201391452809 1103 30-31 -1.0023441376559674 1103 32-33 -1.028604633575 1103 34-35 -1.0716478101343299 1103 36-37 -0.9633467530861708 1103 38-39 -1.01508468861946 1103 40-41 -1.0882689423547447 1103 42-43 -1.1491506821064945 1103 44-45 -1.239800150260912 1103 46-47 -1.2019082095348494 1103 48-49 -1.278972238850585 1103 50-51 -1.3747218264771774 1103 52-53 -1.2719140021184927 1103 54-55 -1.023946136860019 1103 56-57 -0.9870806942647121 1103 58-59 -1.02430536983983 1103 60-61 -1.0648098857343413 1103 62-63 -0.8907699380186855 1103 64-65 -0.9456358509742628 1103 66-67 -0.9590508628170369 1103 68-69 -0.9050229000951262 1103 70-71 -0.9189893283292037 1103 72-73 -0.8840941906278417 1103 74-75 -0.9280314283810398 1103 76-77 -0.9290322404439628 1103 78-79 -1.0667944852877937 1103 80-81 -1.0612921375554194 1103 82-83 -1.0529974682116467 1103 84-85 -1.0204935845722751 1103 86-87 -1.1404035186466537 1103 88-89 -1.1568346127630385 1103 90-91 -1.2569204254189508 1103 92-93 -1.3345407886604157 1103 94-95 -1.2954307410004766 1103 96-97 -1.4302492709489236 1103 98-99 -1.4293484783849486 1103 100 -1.4813437980759758 1104 1 -0.4853229548260174 1104 2 -0.46901525344810935 1104 3 -0.4618714991063868 1104 4 -0.36538191413273324 1104 5 -0.3654515835803238 1104 6 -0.3738367075119413 1104 7 -0.40407325947560935 1104 8 -0.37145015043242324 1104 9 -0.438164270518719 1104 10-11 -0.5697093316956838 1104 12-13 -0.5194242564210114 1104 14-15 -0.6626046146126541 1104 16-17 -0.7356300004471095 1104 18-19 -0.7933074847389179 1104 20-21 -0.7104667113667631 1104 22-23 -0.7050383850384918 1104 24-25 -0.796321821363648 1104 26-27 -0.8283162136486624 1104 28-29 -0.8598246338477225 1104 30-31 -0.9236536514612297 1104 32-33 -0.9172187048751326 1104 34-35 -1.0568182760258225 1104 36-37 -1.0255100131760884 1104 38-39 -0.924550681220218 1104 40-41 -0.9139643448256436 1104 42-43 -0.9093294463855273 1104 44-45 -1.0172366151490237 1104 46-47 -1.0717806085268577 1104 48-49 -1.041048842254746 1104 50-51 -1.1276026623535245 1104 52-53 -0.9718493342060839 1104 54-55 -0.8442520416932311 1104 56-57 -0.7770496899740706 1104 58-59 -0.7822847157814152 1104 60-61 -0.7492403193988793 1104 62-63 -0.8389030142192269 1104 64-65 -0.7822495269814027 1104 66-67 -0.9387315170605817 1104 68-69 -0.9339284831396597 1104 70-71 -0.8974664428731529 1104 72-73 -0.8414726744027377 1104 74-75 -0.7737785723952442 1104 76-77 -0.8132521757856708 1104 78-79 -0.8379592687224218 1104 80-81 -0.9194148373151307 1104 82-83 -0.9284492930213553 1104 84-85 -0.9584550317245011 1104 86-87 -0.9159361933747512 1104 88-89 -0.967547749919003 1104 90-91 -1.0633603227918478 1104 92-93 -1.061943561073246 1104 94-95 -1.0628999558161247 1104 96-97 -1.078696116966558 1104 98-99 -1.0678308154128509 1104 100 -0.9896513345747557 1105 1 -0.5788108518642865 1105 2 -0.552616413039793 1105 3 -0.6119529363104661 1105 4 -0.4519061551698442 1105 5 -0.4539788149961055 1105 6 -0.4457153426372571 1105 7 -0.3670945211454608 1105 8 -0.41841803708499015 1105 9 -0.612361673129378 1105 10-11 -0.596490366468025 1105 12-13 -0.6301444846531226 1105 14-15 -0.886820431804658 1105 16-17 -0.8519839847027768 1105 18-19 -0.8488134823678166 1105 20-21 -0.8762380784012009 1105 22-23 -0.8105549167625625 1105 24-25 -0.8691758291071778 1105 26-27 -0.8769996006197118 1105 28-29 -0.947493270344502 1105 30-31 -0.9423822235095685 1105 32-33 -0.8818326636051097 1105 34-35 -0.8786783618808229 1105 36-37 -0.9185021437485901 1105 38-39 -0.9868524816762019 1105 40-41 -1.0018714635015584 1105 42-43 -1.0170137970679605 1105 44-45 -1.0612102951286033 1105 46-47 -1.1041021213042086 1105 48-49 -1.0447105391032423 1105 50-51 -1.0311882457566668 1105 52-53 -0.9024886148403297 1105 54-55 -0.7693727017429097 1105 56-57 -0.6558010613204388 1105 58-59 -0.6637411732951719 1105 60-61 -0.7001022862680415 1105 62-63 -0.7597381075671876 1105 64-65 -0.763932949585957 1105 66-67 -0.7278510184070512 1105 68-69 -0.7631382270406348 1105 70-71 -0.6825017847860693 1105 72-73 -0.702398563238873 1105 74-75 -0.8002809073348516 1105 76-77 -0.7123664044134372 1105 78-79 -0.7383888296524503 1105 80-81 -0.7262051533389311 1105 82-83 -0.853250551177009 1105 84-85 -0.8542354366250606 1105 86-87 -0.9637669637266555 1105 88-89 -1.008644367184278 1105 90-91 -0.9619987449138847 1105 92-93 -0.9133901938537434 1105 94-95 -1.1052164416230745 1105 96-97 -1.0464218871062663 1105 98-99 -1.10831414890529 1105 100 -1.1409926098628453 1106 1 -0.5490215141780226 1106 2 -0.46548100944286475 1106 3 -0.46371358850450406 1106 4 -0.41674825510927604 1106 5 -0.3398008358477611 1106 6 -0.37678750554769636 1106 7 -0.45189754880421873 1106 8 -0.3680217537507815 1106 9 -0.5234598214691175 1106 10-11 -0.5002159937070587 1106 12-13 -0.6437017543483066 1106 14-15 -0.7501169750577645 1106 16-17 -0.7585136751968875 1106 18-19 -0.7875916942000174 1106 20-21 -0.6591686872637297 1106 22-23 -0.7012264905062793 1106 24-25 -0.6139128821457476 1106 26-27 -0.7731132695221845 1106 28-29 -0.8854593221475753 1106 30-31 -0.8569828581203396 1106 32-33 -0.8097581563209033 1106 34-35 -0.7940963709572699 1106 36-37 -0.8353928735232827 1106 38-39 -0.8787654489462398 1106 40-41 -0.8707261923448613 1106 42-43 -0.7699744203195706 1106 44-45 -0.8347283038081983 1106 46-47 -0.800700095998593 1106 48-49 -0.8857719288954797 1106 50-51 -0.87045848813014 1106 52-53 -0.7844509489808615 1106 54-55 -0.6359571202866263 1106 56-57 -0.6526581981259412 1106 58-59 -0.6498259186871636 1106 60-61 -0.6559608810853703 1106 62-63 -0.64595667939669 1106 64-65 -0.6692901071107613 1106 66-67 -0.6691948022403196 1106 68-69 -0.674270996008886 1106 70-71 -0.7216200494068161 1106 72-73 -0.6623776704221243 1106 74-75 -0.6179594369523471 1106 76-77 -0.6466593817302666 1106 78-79 -0.6677753798024568 1106 80-81 -0.6518466536273877 1106 82-83 -0.5654308658391827 1106 84-85 -0.6569743286523568 1106 86-87 -0.7232130309017037 1106 88-89 -0.7408868919203151 1106 90-91 -0.7531319508796059 1106 92-93 -0.866049420365453 1106 94-95 -0.940350401420968 1106 96-97 -0.834506505044498 1106 98-99 -0.7795097421434285 1106 100 -1.0262861498773717 1107 1 -0.6148828803357986 1107 2 -0.5569414924205915 1107 3 -0.6152764269831792 1107 4 -0.46593513198213543 1107 5 -0.4572019192676322 1107 6 -0.5002523711124098 1107 7 -0.45738066698478264 1107 8 -0.5142670275418197 1107 9 -0.730165433460165 1107 10-11 -0.6806514401347172 1107 12-13 -0.7013951208280531 1107 14-15 -0.8282186642030922 1107 16-17 -0.7674108080219852 1107 18-19 -0.8539089795963193 1107 20-21 -0.8829177331083855 1107 22-23 -0.9682905763421772 1107 24-25 -0.9116297205318702 1107 26-27 -0.9284676994050329 1107 28-29 -1.0339017980373129 1107 30-31 -0.9203576018106716 1107 32-33 -1.0523187158111327 1107 34-35 -1.0748718424257007 1107 36-37 -1.1260661817173983 1107 38-39 -1.051649810360388 1107 40-41 -1.0024552609361805 1107 42-43 -1.05431676486495 1107 44-45 -0.9946869665303311 1107 46-47 -0.9887794431879655 1107 48-49 -0.9715319470539114 1107 50-51 -0.9784149417316712 1107 52-53 -0.9781114746896264 1107 54-55 -0.828602862732474 1107 56-57 -0.79648008840784 1107 58-59 -0.8563188321423212 1107 60-61 -0.7985318352191015 1107 62-63 -0.7650605890971747 1107 64-65 -0.7565899528252586 1107 66-67 -0.8397217877072691 1107 68-69 -0.7927093941731655 1107 70-71 -0.8329953345729848 1107 72-73 -0.7516516993355324 1107 74-75 -0.7817910610143315 1107 76-77 -0.7419612347048954 1107 78-79 -0.7373899258027841 1107 80-81 -0.8205267778985252 1107 82-83 -0.8233820928572975 1107 84-85 -0.8139175093430389 1107 86-87 -0.8677928770699843 1107 88-89 -0.9134060227462477 1107 90-91 -0.8876422281163165 1107 92-93 -0.8776088581885588 1107 94-95 -0.9069396019580864 1107 96-97 -0.9503577894334825 1107 98-99 -0.9704164076084183 1107 100 -0.9636254020500701 1108 1 -0.5563774020562811 1108 2 -0.5189552505785109 1108 3 -0.5799982279488951 1108 4 -0.37783753343595805 1108 5 -0.3673748704802051 1108 6 -0.37888019412773843 1108 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0.7268165363801664 2116 92-93 0.6831562208099022 2116 94-95 0.6273177678540627 2116 96-97 0.7332782841691277 2116 98-99 0.8949482080689606 2116 100 0.530264337846603 2201 1 0.8053633269662903 2201 2 0.7955561609217661 2201 3 0.81410515720248 2201 4 0.610853380291708 2201 5 0.6556640954076514 2201 6 0.6601515705419487 2201 7 0.6951354917771084 2201 8 0.7272083048556937 2201 9 0.8921030387835316 2201 10-11 0.8886397140312567 2201 12-13 0.9071798736020611 2201 14-15 1.0434750899743221 2201 16-17 1.0380304244876655 2201 18-19 1.0440385015360647 2201 20-21 1.049343452626374 2201 22-23 1.0637967710642116 2201 24-25 1.076212020998561 2201 26-27 1.0204592449727983 2201 28-29 1.0525856237559807 2201 30-31 0.9823159899816929 2201 32-33 0.9655630589072999 2201 34-35 0.9623909351403697 2201 36-37 0.980713665271999 2201 38-39 0.9204907066469445 2201 40-41 0.921971862408526 2201 42-43 0.908192411753447 2201 44-45 0.9017760481973056 2201 46-47 0.8481566132827822 2201 48-49 0.8458356935416163 2201 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0.1269569365559775 2202 76-77 0.15872810219443068 2202 78-79 0.47977169991767 2202 80-81 0.5529514137869711 2202 82-83 0.6879127198744079 2202 84-85 0.6660998106218905 2202 86-87 0.7438335892746615 2202 88-89 0.75809362687637 2202 90-91 0.7524550016073128 2202 92-93 0.6973999777476152 2202 94-95 0.7268595560651718 2202 96-97 0.6770255609087066 2202 98-99 0.6854673504873645 2202 100 0.7187908554604725 2203 1 0.8326162216402189 2203 2 0.8389810228488592 2203 3 0.8463076721270646 2203 4 0.6768588865399536 2203 5 0.7193236122683544 2203 6 0.715815541989258 2203 7 -0.24019994869931338 2203 8 0.4618737212308801 2203 9 0.8014595979174928 2203 10-11 0.9039524857040035 2203 12-13 0.9633605604272262 2203 14-15 1.1463867446667706 2203 16-17 1.1859175104577488 2203 18-19 1.1628231369626363 2203 20-21 1.1865233768796486 2203 22-23 1.0888016022488145 2203 24-25 1.1485137262634808 2203 26-27 1.1735157635821025 2203 28-29 1.189892806099735 2203 30-31 1.2106528149636304 2203 32-33 1.2241984432846849 2203 34-35 1.1987985730479025 2203 36-37 1.2060344832762055 2203 38-39 1.2183393905593363 2203 40-41 1.1764563020874803 2203 42-43 1.0761930149353134 2203 44-45 1.1756959138200571 2203 46-47 1.2254393154541816 2203 48-49 1.1423428121814467 2203 50-51 1.2312773049936538 2203 52-53 1.113530695118655 2203 54-55 0.5487339749710358 2203 56-57 0.6475440710204339 2203 58-59 0.9678902001859342 2203 60-61 1.0256814137192194 2203 62-63 1.0758275672580595 2203 64-65 1.1568165651574063 2203 66-67 1.1055340777113045 2203 68-69 1.0020541763215718 2203 70-71 1.0053627417775886 2203 72-73 1.0056516972312508 2203 74-75 1.0094622765655004 2203 76-77 0.9023118262774616 2203 78-79 0.9189997731479735 2203 80-81 0.9444049601228102 2203 82-83 1.0331643500256398 2203 84-85 0.9268926359271497 2203 86-87 0.9122861779109677 2203 88-89 0.8546648105288419 2203 90-91 0.8891185446766237 2203 92-93 0.9132140812642682 2203 94-95 0.8344850667455184 2203 96-97 0.8808098651102121 2203 98-99 0.8025531388940301 2203 100 0.9165263837564694 2204 1 0.708337306697981 2204 2 0.7210237074121189 2204 3 0.7713242144361239 2204 4 0.6030528068647243 2204 5 0.5873643189836883 2204 6 0.6179080073393379 2204 7 0.6801102323106178 2204 8 0.6296406441745859 2204 9 0.8551253083513686 2204 10-11 0.8399555250873973 2204 12-13 0.8649403149568258 2204 14-15 1.0018421256625558 2204 16-17 1.0052204051107267 2204 18-19 1.0207330668327117 2204 20-21 1.0398869466686804 2204 22-23 1.067164892147666 2204 24-25 1.035551146972189 2204 26-27 1.0705990344599456 2204 28-29 1.0631626929684472 2204 30-31 1.1044701913262216 2204 32-33 1.0980725490675596 2204 34-35 1.1459681748508714 2204 36-37 1.1006839011590088 2204 38-39 1.0979693338436718 2204 40-41 1.1172574427726971 2204 42-43 -0.11752847585124471 2204 44-45 0.8176922047474378 2204 46-47 1.04979240449169 2204 48-49 1.1375803049131008 2204 50-51 1.0676292778611298 2204 52-53 0.35047774488748473 2204 54-55 0.5135624717061731 2204 56-57 0.875443566904643 2204 58-59 0.9699044098422931 2204 60-61 1.0336636016363983 2204 62-63 0.9901540641699 2204 64-65 1.01847282502753 2204 66-67 0.9593925972899555 2204 68-69 0.9433355285794462 2204 70-71 0.8932913487990533 2204 72-73 0.9497273050679453 2204 74-75 0.9286276027981302 2204 76-77 0.8691654803732547 2204 78-79 0.872385215954381 2204 80-81 0.856229390805396 2204 82-83 0.8921817493424236 2204 84-85 0.827666426704031 2204 86-87 0.8960448947306254 2204 88-89 0.8758126964367783 2204 90-91 0.8573583748590998 2204 92-93 0.8735549628677788 2204 94-95 0.8667830415369764 2204 96-97 0.8558558086826906 2204 98-99 0.8233379352724874 2204 100 0.6034532766345357 2205 1 0.8188704577248807 2205 2 0.8089577848986984 2205 3 0.8587946491925003 2205 4 0.6849758379790956 2205 5 0.5956163846203566 2205 6 0.7043996407763657 2205 7 0.7225991914965277 2205 8 0.6988607918936154 2205 9 0.8654734374078004 2205 10-11 0.8847969915425367 2205 12-13 0.8928373515245056 2205 14-15 1.1397224878615972 2205 16-17 1.1484712320800128 2205 18-19 1.1351648122897444 2205 20-21 1.181945500699193 2205 22-23 1.1225665697479528 2205 24-25 1.1672790165119338 2205 26-27 1.1563149683278056 2205 28-29 1.1738783727506998 2205 30-31 1.1797952700530558 2205 32-33 1.1654158536733306 2205 34-35 1.1867785174059193 2205 36-37 1.130527185914353 2205 38-39 1.1338673438768794 2205 40-41 1.2343976456282988 2205 42-43 1.2446499827701132 2205 44-45 1.1896944549931803 2205 46-47 1.1803251267491106 2205 48-49 1.1944066326295513 2205 50-51 1.1770781105759198 2205 52-53 1.0913219602313902 2205 54-55 1.1066017962195573 2205 56-57 1.0546101184387666 2205 58-59 1.100332734671703 2205 60-61 1.082514920261353 2205 62-63 1.0063088833006475 2205 64-65 0.9948158421989532 2205 66-67 0.9466772557591909 2205 68-69 0.9622529253452541 2205 70-71 0.9165578179141534 2205 72-73 0.929904855333227 2205 74-75 0.9706487030206148 2205 76-77 0.9886431994466633 2205 78-79 0.9440908530026064 2205 80-81 0.8560615354944545 2205 82-83 0.9217193691001278 2205 84-85 0.9657985480489693 2205 86-87 0.9607056745511393 2205 88-89 0.9120797499487985 2205 90-91 0.9825716717946698 2205 92-93 0.9782416117802093 2205 94-95 1.0813733761200943 2205 96-97 0.9948297077376544 2205 98-99 0.911919065604117 2205 100 1.0526448714823147 2206 1 0.8336361094525593 2206 2 0.8340306166893079 2206 3 0.8374548783287459 2206 4 0.6472977016505723 2206 5 0.5733767086812733 2206 6 0.6817941434980241 2206 7 0.7201345553503131 2206 8 0.6985218096594608 2206 9 0.885797257394664 2206 10-11 0.9224981041366789 2206 12-13 0.9726790072290612 2206 14-15 1.1569887298568489 2206 16-17 1.163049339336844 2206 18-19 1.1593325350133625 2206 20-21 1.2002541552264248 2206 22-23 1.2023975926053652 2206 24-25 1.1953444727686886 2206 26-27 1.1966867887130022 2206 28-29 1.2407315800317846 2206 30-31 1.2480989775758786 2206 32-33 1.260000755030383 2206 34-35 1.2964453495081258 2206 36-37 1.2427881516402834 2206 38-39 1.3291627105575188 2206 40-41 1.3333792694343103 2206 42-43 1.3472868565845602 2206 44-45 1.2998298471155607 2206 46-47 1.2958088811399833 2206 48-49 1.3066569735391909 2206 50-51 1.2743059169167594 2206 52-53 1.2404292186754304 2206 54-55 1.2244895538291942 2206 56-57 1.251392172377173 2206 58-59 1.1955613007310717 2206 60-61 1.126961241248047 2206 62-63 1.148235412254266 2206 64-65 1.2167101353153882 2206 66-67 1.1866585519064685 2206 68-69 1.127555142342068 2206 70-71 1.1276059323505692 2206 72-73 1.1253783864503717 2206 74-75 1.211801973424194 2206 76-77 1.1889203521565221 2206 78-79 1.1524672959458115 2206 80-81 1.1102190269134837 2206 82-83 1.0924052278952736 2206 84-85 1.0540898238139498 2206 86-87 1.137316583017114 2206 88-89 1.1500064250639177 2206 90-91 1.155602242033293 2206 92-93 1.1259058788285046 2206 94-95 1.1453943350358635 2206 96-97 1.129969235748458 2206 98-99 1.1429302220627697 2206 100 1.1990490738661919 2207 1 0.841748934319309 2207 2 0.7984926399965495 2207 3 0.8567726148502324 2207 4 0.6796014719161434 2207 5 0.6355070018785938 2207 6 0.6466819088429858 2207 7 0.7444319362585574 2207 8 0.7422082004441535 2207 9 0.9098318000119079 2207 10-11 0.8897672631689844 2207 12-13 0.9496822464179786 2207 14-15 1.167326308367869 2207 16-17 1.161077435274386 2207 18-19 1.0931728534676424 2207 20-21 1.1293471083035982 2207 22-23 1.1897233916522723 2207 24-25 1.2161975759166097 2207 26-27 1.2416770738035723 2207 28-29 1.2338177798874028 2207 30-31 1.191429162923619 2207 32-33 1.2344157085029295 2207 34-35 1.2755660289643274 2207 36-37 1.2690679235693452 2207 38-39 1.3044739668030658 2207 40-41 1.366140290064962 2207 42-43 1.292961161837809 2207 44-45 1.335699539113115 2207 46-47 1.4752243437757073 2207 48-49 1.4303257348452902 2207 50-51 1.4119369481397115 2207 52-53 1.358239613898995 2207 54-55 1.2909667861680916 2207 56-57 1.273584237080847 2207 58-59 1.1528229120004951 2207 60-61 1.199897134993627 2207 62-63 1.2087206528331649 2207 64-65 1.1892064736586434 2207 66-67 1.2055036635175966 2207 68-69 1.223061319350343 2207 70-71 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2311 58-59 1.275860248747172 2311 60-61 1.2541761982474497 2311 62-63 1.2596522984312486 2311 64-65 1.2332772678824782 2311 66-67 1.2401001978856883 2311 68-69 1.193264117458952 2311 70-71 1.1828638075722253 2311 72-73 1.1590482180250419 2311 74-75 1.1929218703391484 2311 76-77 1.1404967012874536 2311 78-79 1.1837898651985057 2311 80-81 1.130034518656636 2311 82-83 1.1368199646992956 2311 84-85 1.2058415230350512 2311 86-87 1.2213027708694177 2311 88-89 1.265093646228589 2311 90-91 1.2917274227631559 2311 92-93 1.3396494149206397 2311 94-95 1.316572240153132 2311 96-97 1.4016619005014093 2311 98-99 1.440262829809523 2311 100 1.6242436650798169 2312 1 0.6293592548793434 2312 2 0.5858842563200746 2312 3 0.6770994847978393 2312 4 0.3754095281273564 2312 5 0.44999883857408207 2312 6 0.5352903102522291 2312 7 0.3851734956155468 2312 8 0.5163625106381105 2312 9 0.7166755326683543 2312 10-11 0.6610765183480609 2312 12-13 0.7915934934522255 2312 14-15 0.9972115667523553 2312 16-17 1.0005867883637265 2312 18-19 1.0083928211644348 2312 20-21 1.0242346540050775 2312 22-23 1.0233647791913327 2312 24-25 1.0031357398269876 2312 26-27 1.049593850508444 2312 28-29 1.1133375003175203 2312 30-31 1.1259817090441402 2312 32-33 1.145259849857645 2312 34-35 1.0987408566133539 2312 36-37 1.1789382927551841 2312 38-39 1.1534692994942262 2312 40-41 1.1861097601357216 2312 42-43 1.2539806676802243 2312 44-45 1.256710630331149 2312 46-47 1.2068810536997958 2312 48-49 1.252868674023354 2312 50-51 1.252860648438066 2312 52-53 1.1715206608758706 2312 54-55 1.0488169171900807 2312 56-57 0.9763818569201135 2312 58-59 0.9906630092267719 2312 60-61 1.0126315269314574 2312 62-63 1.0206402342720935 2312 64-65 1.0304021292974213 2312 66-67 0.9953178092485047 2312 68-69 0.9856078916364268 2312 70-71 0.9691183491095501 2312 72-73 0.9778005991246985 2312 74-75 1.0391925321536775 2312 76-77 0.992657153048988 2312 78-79 0.9542720293434996 2312 80-81 1.030397196724934 2312 82-83 1.0309563613814667 2312 84-85 1.0911113595474866 2312 86-87 1.1601097793264223 2312 88-89 1.1009171283925987 2312 90-91 1.1117632570665954 2312 92-93 1.2276170835736586 2312 94-95 1.2310805311500808 2312 96-97 1.2679020649670214 2312 98-99 0.9265122475858583 2312 100 1.2402211737853541 2313 1 0.5973489608453697 2313 2 0.5804767423650254 2313 3 0.627347333053482 2313 4 0.5280087637117177 2313 5 0.3999372708456832 2313 6 0.4991423982205845 2313 7 0.5494830327622893 2313 8 0.5705267664457097 2313 9 0.6643024094234704 2313 10-11 0.6918986098956239 2313 12-13 0.7750049796827199 2313 14-15 1.0110273130454104 2313 16-17 1.0123527251354645 2313 18-19 0.9704542904552653 2313 20-21 0.9639179077342632 2313 22-23 1.0535156082066237 2313 24-25 1.0108591559107794 2313 26-27 1.0547736490877213 2313 28-29 1.140296067381776 2313 30-31 1.1209119247197563 2313 32-33 1.1507912386931878 2313 34-35 1.1621385016316808 2313 36-37 1.2283573469728921 2313 38-39 1.2689556108916875 2313 40-41 1.2760446791455635 2313 42-43 1.271140857314137 2313 44-45 1.231793429369013 2313 46-47 1.3321010429544557 2313 48-49 1.3216025528382076 2313 50-51 1.383704918414054 2313 52-53 1.2172979463452833 2313 54-55 1.0974863485210165 2313 56-57 1.0535058322080602 2313 58-59 1.0562729299973839 2313 60-61 0.999197804317717 2313 62-63 1.1173843247850925 2313 64-65 1.0978934765413797 2313 66-67 1.0907063634093888 2313 68-69 1.070164138497617 2313 70-71 1.0932152779632105 2313 72-73 1.11163758657667 2313 74-75 1.0636508122273298 2313 76-77 0.875842719646954 2313 78-79 1.0083725277007858 2313 80-81 1.0879148888642476 2313 82-83 1.0989481971161261 2313 84-85 1.108729777752334 2313 86-87 1.174798894915444 2313 88-89 1.2076123843957838 2313 90-91 1.1787422964604382 2313 92-93 1.2534986900210292 2313 94-95 1.2801067915113258 2313 96-97 1.3062598903065243 2313 98-99 1.2983811700905115 2313 100 1.4613140397199302 2314 1 0.6076999822425471 2314 2 0.5916509803920285 2314 3 0.5868046587182683 2314 4 0.44582512474065794 2314 5 0.4857442210756986 2314 6 0.5398186702226511 2314 7 0.595955338904119 2314 8 0.5542334466575483 2314 9 0.7126914342484696 2314 10-11 0.6878085837200558 2314 12-13 0.7459795572632615 2314 14-15 0.8951048624961757 2314 16-17 0.9141312277202545 2314 18-19 0.8960117997270771 2314 20-21 0.9392094243019073 2314 22-23 0.9910111366239533 2314 24-25 0.9576791138599461 2314 26-27 1.0063606412683441 2314 28-29 0.9649199964916662 2314 30-31 1.0531841156429849 2314 32-33 1.0263913494464632 2314 34-35 1.0445197448867276 2314 36-37 1.0974870462826587 2314 38-39 1.116901018525482 2314 40-41 1.1393350413001926 2314 42-43 1.1860752502531966 2314 44-45 1.2856845678989615 2314 46-47 1.2838427344963463 2314 48-49 1.2392951154040261 2314 50-51 1.273560935818324 2314 52-53 1.199421762847848 2314 54-55 1.0823288455296094 2314 56-57 0.9800011976579484 2314 58-59 1.0026875711658576 2314 60-61 1.035969177146633 2314 62-63 1.0207811435960963 2314 64-65 1.046175534149235 2314 66-67 1.0121152322344145 2314 68-69 1.0203777667068081 2314 70-71 1.0068617019804265 2314 72-73 1.0687717214737376 2314 74-75 1.0178375999720686 2314 76-77 1.0342063845781055 2314 78-79 1.0011076291111323 2314 80-81 1.0365765542623109 2314 82-83 1.0919755347231437 2314 84-85 1.121508009021678 2314 86-87 1.1914286029943817 2314 88-89 1.184513211387337 2314 90-91 1.1889667494491185 2314 92-93 1.2457362591325527 2314 94-95 1.2987724372791334 2314 96-97 1.3649237560712386 2314 98-99 1.4972254430934342 2314 100 1.4926626389877846 2315 1 0.4910336478360122 2315 2 0.5107531138343404 2315 3 0.5640504880229464 2315 4 0.2603258706646585 2315 5 0.3408903490822368 2315 6 0.3850361178667612 2315 7 0.4340382477751277 2315 8 0.4384786679261836 2315 9 0.5435024978463829 2315 10-11 0.4838862528487695 2315 12-13 0.5221714574257419 2315 14-15 0.7505412612614961 2315 16-17 0.768391301016841 2315 18-19 0.5901820464966718 2315 20-21 0.6991581648698713 2315 22-23 0.6056284643221588 2315 24-25 0.7069689493579503 2315 26-27 0.7474528716973907 2315 28-29 0.790220858042197 2315 30-31 0.7945318541143891 2315 32-33 0.8750733980959851 2315 34-35 0.8641366323402551 2315 36-37 0.908790245283221 2315 38-39 0.9502212691160778 2315 40-41 1.0184414442852159 2315 42-43 1.1326265977341592 2315 44-45 1.069671872239276 2315 46-47 1.1758463129379777 2315 48-49 1.1460475965565777 2315 50-51 0.9834751213324466 2315 52-53 1.0456284319732347 2315 54-55 1.0027902136439408 2315 56-57 0.96131318493903 2315 58-59 0.8902621635657368 2315 60-61 0.8603596043999389 2315 62-63 0.856026301735298 2315 64-65 0.8660455622916672 2315 66-67 0.8790104108076164 2315 68-69 0.9630899729473228 2315 70-71 0.9486184942082048 2315 72-73 0.6750421347056701 2315 74-75 0.8379582353198884 2315 76-77 0.8699427477915904 2315 78-79 0.8842462580064705 2315 80-81 0.6011386437133837 2315 82-83 0.6083597387441522 2315 84-85 0.8889632535817462 2315 86-87 1.035432304372268 2315 88-89 1.0525536906393533 2315 90-91 1.138907025690191 2315 92-93 1.2123527129866112 2315 94-95 1.1358397099882822 2315 96-97 1.0704384020979063 2315 98-99 0.4805079406264028 2315 100 0.986595070567116 2316 1 0.31184421883132885 2316 2 0.23630056875663286 2316 3 0.32020972837337425 2316 4 0.24681464345182746 2316 5 0.18537524151156504 2316 6 0.2056837932940283 2316 7 0.11441413260929068 2316 8 0.23088813176137535 2316 9 0.2631574042476501 2316 10-11 0.2504035985780604 2316 12-13 0.11093480857163485 2316 14-15 0.0961272714457806 2316 16-17 0.2789975572661305 2316 18-19 0.2739275850784253 2316 20-21 0.2961561021881849 2316 22-23 0.3252880887352987 2316 24-25 0.34794620688525413 2316 26-27 0.42600818335104407 2316 28-29 0.4516940696673757 2316 30-31 0.348442589329629 2316 32-33 0.5021512270821304 2316 34-35 0.5151638345757661 2316 36-37 0.5104851703578888 2316 38-39 0.663259784647444 2316 40-41 0.7571557943252785 2316 42-43 0.7096595642213828 2316 44-45 0.6362430198584192 2316 46-47 0.4207366233003853 2316 48-49 0.4078953521072748 2316 50-51 0.4298901772532986 2316 52-53 0.5604501076786192 2316 54-55 0.2712292801066525 2316 56-57 0.1194479479127395 2316 58-59 0.22258443523051596 2316 60-61 0.28361898910497274 2316 62-63 0.4104890443404372 2316 64-65 0.36300277571796613 2316 66-67 0.3541921784231832 2316 68-69 0.26580291255015 2316 70-71 -0.027629544092121705 2316 72-73 -0.0790703162104549 2316 74-75 0.15618871599424722 2316 76-77 0.33645752758459224 2316 78-79 0.4159559330761624 2316 80-81 0.5908422035775089 2316 82-83 0.5683921357590513 2316 84-85 0.6149107462481318 2316 86-87 0.637497138132705 2316 88-89 0.6269711403304434 2316 90-91 0.4635982856145695 2316 92-93 0.4559982131620828 2316 94-95 0.6755114025791826 2316 96-97 0.6017751886848473 2316 98-99 0.5272616173647826 2316 100 0.3796349270071211 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 32.0 1 400.0 2 178.0 3 159.0 4 119.0 5 98.0 6 99.0 7 411.0 8 1476.0 9 2973.0 10 4330.0 11 6557.0 12 9241.0 13 11937.0 14 13943.0 15 16924.0 16 20802.0 17 25916.0 18 31950.0 19 39404.0 20 48626.0 21 60041.0 22 65925.0 23 65698.0 24 64986.0 25 68350.0 26 75825.0 27 88771.0 28 105272.0 29 126221.0 30 153947.0 31 192836.0 32 245420.0 33 325952.0 34 468077.0 35 655799.0 36 998389.0 37 1418408.0 38 1296333.0 39 382963.0 40 348.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.454053178180594 20.763458873378912 12.873108991394854 22.909378957045636 2 15.445887851562043 19.96242534358639 41.78309131993541 22.808595484916154 3 19.33128913227838 26.659442607495354 28.545178610093647 25.46408965013262 4 12.203346107191868 16.39295085999762 39.99517896676236 31.408524066048155 5 12.909205658588105 38.51982734570739 34.863209154359645 13.707757841344865 6 29.591275113176664 38.29845020981204 18.352648657582264 13.757626019429031 7 27.29012071551857 32.0560137831656 22.998380052148022 17.655485449167806 8 26.002352928236533 35.73242893799965 19.993671376048443 18.271546757715374 9 26.255003184469544 15.667181267169312 21.678672993399964 36.39914255496118 10-11 25.518100442781368 26.45051662525689 27.48858220218711 20.542800729774637 12-13 25.60581333202109 24.051122218336097 28.101872195723164 22.24119225391965 14-15 23.01067253355986 25.339101643022367 25.928918098676633 25.721307724741138 16-17 21.61027877043653 28.159096494888868 27.088857605287707 23.141767129386885 18-19 20.985278950285313 28.190233298672045 29.352472998064037 21.472014752978602 20-21 22.521882436893694 27.28876146968438 28.92775653928576 21.26159955413616 22-23 22.754224764424634 27.138353814209403 28.476569766405017 21.630851654960942 24-25 22.159204811932725 26.97993449090319 29.319458491215595 21.541402205948486 26-27 22.140908899639662 27.329797969438886 28.313952549944116 22.215340580977337 28-29 21.404096457826203 27.674068021871385 28.90933762776585 22.01249789253656 30-31 22.340511910242743 27.358197333005545 28.861049470409974 21.44024128634174 32-33 22.399831451483127 27.500179213200127 28.143972237143927 21.956017098172815 34-35 22.460148045310188 27.443841576744017 28.743995915135876 21.35201446280992 36-37 22.098288486084275 27.050620809007025 28.40059175418123 22.45049895072747 38-39 22.294275078996876 27.265703690703003 28.843963637824615 21.596057592475507 40-41 22.14917128318025 26.407066254488694 29.344095110706103 22.09966735162495 42-43 22.048553945127 26.5456148865774 28.795865403560565 22.609965764735033 44-45 22.12174814229918 27.355078185586777 28.65938156540376 21.863792106710285 46-47 22.10421160013816 27.19807826985063 28.024935225648957 22.672774904362257 48-49 22.648289890011608 27.14191416163717 28.09228977241844 22.117506175932782 50-51 22.40602353891229 26.920625463654428 28.126986964525834 22.546364032907444 52-53 22.035985210328967 27.397226859132235 27.887811859908435 22.678976070630366 54-55 21.85368187974186 27.334913456631455 27.98323351993226 22.82817114369443 56-57 22.698978735422028 26.99618863154297 27.528777724739296 22.77605490829571 58-59 21.84211229481851 27.372786470808908 28.282757567047355 22.502343667325228 60-61 21.963632862334055 27.915985200179687 27.651220359247585 22.469161578238673 62-63 22.24728899927826 27.22549688081338 27.446501972184784 23.080712147723574 64-65 21.65524558612643 27.281633864894783 27.792741786775043 23.27037876220374 66-67 22.050886813711493 27.076708492529832 27.658666442589052 23.213738251169623 68-69 21.710876214206877 27.13457329119928 27.939878042108862 23.214672452484983 70-71 21.73489367393151 26.763425493426237 28.009807154214233 23.491873678428018 72-73 22.239852598585166 26.83432350856992 28.15223761836787 22.77358627447704 74-75 22.4923185130866 27.049315819164875 27.70866650204869 22.749699165699834 76-77 22.1724774998074 26.955987398045423 27.511751159820637 23.35978394232654 78-79 22.1290558091805 27.013313294891233 27.46388220474834 23.393748691179926 80-81 21.820042584887293 27.44429421791065 27.863770935684016 22.87189226151804 82-83 22.653354314502558 26.6037889454143 28.733069835222636 22.0097869048605 84-85 22.71633742855262 26.900540116745958 27.759924400288856 22.623198054412565 86-87 21.60710025390773 27.472442817900056 28.141039488730975 22.779417439461245 88-89 22.625288808129902 26.37698923427608 28.041214805584026 22.95650715200999 90-91 22.38465334928941 26.58292168518866 27.994066233142117 23.038358732379812 92-93 22.682720566675236 26.66014259356998 27.959543980259742 22.697592859495035 94-95 21.773887440021433 26.641250338427387 28.527808986051472 23.057053235499716 96-97 22.149558876623754 26.503563117487083 28.10074538649981 23.246132619389353 98-99 21.652528854234497 26.801085497521342 28.044735535804737 23.501650112439417 100 22.346677539295204 26.345357372105983 27.329378189129315 23.978586899469494 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 908.0 1 633.0 2 648.0 3 919.5 4 715.0 5 580.5 6 799.0 7 1491.5 8 2459.0 9 2927.5 10 2722.5 11 2559.5 12 2863.0 13 3330.0 14 3869.5 15 4543.5 16 5236.0 17 5813.5 18 6525.0 19 7233.5 20 7862.5 21 8665.5 22 9849.0 23 11410.5 24 13357.5 25 16034.5 26 19951.5 27 24818.0 28 31104.5 29 42793.5 30 60334.0 31 78962.0 32 103494.5 33 131712.5 34 157229.0 35 182381.5 36 207228.0 37 238240.5 38 251778.0 39 246002.5 40 235900.5 41 222004.0 42 217854.5 43 225459.5 44 239350.0 45 266606.5 46 294704.5 47 313525.5 48 325919.5 49 321696.0 50 306695.5 51 307453.0 52 301429.0 53 278552.0 54 255908.5 55 216401.0 56 185147.0 57 150591.0 58 115868.0 59 108568.0 60 89994.0 61 61133.0 62 44510.5 63 37176.5 64 27568.5 65 14067.5 66 8127.5 67 5434.0 68 4010.5 69 3153.5 70 2496.5 71 1890.0 72 1468.0 73 1236.0 74 958.0 75 708.5 76 525.5 77 356.5 78 269.0 79 209.0 80 144.5 81 102.0 82 93.0 83 75.0 84 52.5 85 44.5 86 37.0 87 31.5 88 23.0 89 13.5 90 12.5 91 13.0 92 10.0 93 6.0 94 5.5 95 4.5 96 3.5 97 2.5 98 1.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05842030371228966 2 0.13402139155613085 3 0.01758951484509952 4 0.017208972456623808 5 0.020055993288923567 6 0.05340278184942473 7 0.05873037528808468 8 0.049991994515679476 9 0.04091535384240697 10-11 0.03959754964527812 12-13 0.041655297375554186 14-15 0.018590200385165276 16-17 0.017652938576512133 18-19 0.01756837360129531 20-21 0.021007349260112846 22-23 0.02553157543421296 24-25 0.024946667688963257 26-27 0.019485179706210002 28-29 0.018322411296978664 30-31 0.016321040216847146 32-33 0.022966437852635947 34-35 0.02300167325897629 36-37 0.023255368184626764 38-39 0.021155337966742287 40-41 0.024805726063601884 42-43 0.032465903401992575 44-45 0.042789877459713244 46-47 0.04794129386667148 48-49 0.04069689432309684 50-51 0.028611149948358987 52-53 0.04071803556690105 54-55 0.037370671964568405 56-57 0.03948479634498902 58-59 0.04443184739517326 60-61 0.02355839267915372 62-63 0.026222189398483694 64-65 0.025651375815770126 66-67 0.033198799853871724 68-69 0.04019655155306395 70-71 0.041810333163451695 72-73 0.04523521465973309 74-75 0.027420193214055377 76-77 0.020781842659534644 78-79 0.02026035864569756 80-81 0.025010091420375878 82-83 0.020767748496998507 84-85 0.013093476996071675 86-87 0.014425375355736663 88-89 0.018688859522918235 90-91 0.021098961316597737 92-93 0.019844580850881505 94-95 0.023459733541400758 96-97 0.024932573526427117 98-99 0.02794872430916053 100 0.029527270513207922 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7095136.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.52764441910226 #Duplication Level Percentage of deduplicated Percentage of total 1 79.699237649372 55.41300255759064 2 13.392872310462273 18.623497275045224 3 3.811658932132404 7.950470006405904 4 1.456083544327692 4.049522356580876 5 0.641266838814803 2.2292886373438696 6 0.33614151901265193 1.402267680504514 7 0.18581569754103125 0.9043529422284192 8 0.10465840079789107 0.5821321660918126 9 0.06731091851444335 0.4211972647195845 >10 0.26507372889435454 3.1564532700366716 >50 0.020715711166551565 1.018987921700312 >100 0.01727886876003465 2.3690566052048583 >500 0.0013578390322462732 0.6534019320679008 >1k 4.526067185890064E-4 0.4712434677424481 >5k 2.514481767045029E-5 0.158934311324877 >10k+ 5.028963534090058E-5 0.596191605412051 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19120 0.26948038769094773 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14982 0.21115874311641103 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9091 0.1281300316160254 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.4094162536137433E-5 2 0.0 0.0 0.0 0.0 1.4094162536137433E-5 3 0.0 0.0 0.0 0.0 1.4094162536137433E-5 4 0.0 0.0 0.0 0.0 1.4094162536137433E-5 5 0.0 0.0 0.0 0.0 1.4094162536137433E-5 6 0.0 0.0 0.0 0.0 1.4094162536137433E-5 7 0.0 0.0 0.0 0.0 1.4094162536137433E-5 8 0.0 0.0 0.0 0.0 1.4094162536137433E-5 9 0.0 0.0 0.0 1.4094162536137433E-5 1.4094162536137433E-5 10-11 0.0 1.4094162536137433E-5 0.0 4.228248760841229E-5 1.4094162536137433E-5 12-13 0.0 1.4094162536137433E-5 0.0 7.047081268068716E-5 3.5235406340343586E-5 14-15 0.0 1.4094162536137433E-5 0.0 7.751789394875588E-5 4.22824876084123E-5 16-17 0.0 1.4094162536137433E-5 0.0 1.1980038155716818E-4 5.637665014454973E-5 18-19 0.0 1.4094162536137433E-5 0.0 1.691299504336492E-4 5.637665014454973E-5 20-21 0.0 1.4094162536137433E-5 0.0 2.818832507227487E-4 5.637665014454973E-5 22-23 0.0 1.4094162536137433E-5 0.0 6.69472720466528E-4 5.637665014454973E-5 24-25 0.0 1.4094162536137433E-5 0.0 0.0013600866847372623 5.637665014454973E-5 26-27 0.0 1.4094162536137433E-5 0.0 0.0025933259066492874 5.637665014454973E-5 28-29 0.0 1.4094162536137433E-5 0.0 0.006899092561439273 5.637665014454973E-5 30-31 0.0 1.4094162536137433E-5 0.0 0.022437906757530792 5.637665014454973E-5 32-33 0.0 1.4094162536137433E-5 0.0 0.05862466906906365 5.637665014454973E-5 34-35 0.0 1.4094162536137433E-5 0.0 0.11249255828218092 5.637665014454973E-5 36-37 0.0 2.114124380420615E-5 0.0 0.2021948557434276 5.637665014454973E-5 38-39 0.0 2.8188325072274866E-5 0.0 0.3310930192176725 5.637665014454973E-5 40-41 0.0 2.8188325072274866E-5 0.0 0.4652201169928244 5.637665014454973E-5 42-43 0.0 2.8188325072274866E-5 0.0 0.5959223332716949 5.637665014454973E-5 44-45 0.0 2.8188325072274866E-5 0.0 0.7527480798112961 5.637665014454973E-5 46-47 0.0 2.8188325072274866E-5 0.0 0.9322372397090063 5.637665014454973E-5 48-49 0.0 2.8188325072274866E-5 0.0 1.12849845302472 5.637665014454973E-5 50-51 0.0 2.8188325072274866E-5 0.0 1.3318349359335748 5.637665014454973E-5 52-53 0.0 2.8188325072274866E-5 0.0 1.525277316742061 5.637665014454973E-5 54-55 0.0 2.8188325072274866E-5 0.0 1.7351464439864155 5.637665014454973E-5 56-57 0.0 2.8188325072274866E-5 0.0 1.9806597082846615 5.637665014454973E-5 58-59 0.0 5.637665014454973E-5 0.0 2.2575747667134216 5.637665014454973E-5 60-61 0.0 5.637665014454973E-5 0.0 2.538083836588897 5.637665014454973E-5 62-63 0.0 5.637665014454973E-5 0.0 2.820093934774471 5.637665014454973E-5 64-65 1.4094162536137433E-5 5.637665014454973E-5 0.0 3.1044718522661157 5.637665014454973E-5 66-67 1.4094162536137433E-5 5.637665014454973E-5 0.0 3.4201035751816455 5.637665014454973E-5 68-69 1.4094162536137433E-5 7.047081268068717E-5 0.0 3.764741084596546 5.637665014454973E-5 70-71 1.4094162536137433E-5 9.161205648489332E-5 0.0 4.136883352200719 5.637665014454973E-5 72-73 1.4094162536137433E-5 9.865913775296202E-5 0.0 4.511809216905778 5.637665014454973E-5 74-75 1.4094162536137433E-5 9.865913775296202E-5 0.0 4.872535212855681 5.637665014454973E-5 76-77 1.4094162536137433E-5 9.865913775296202E-5 0.0 5.270371138763232 5.637665014454973E-5 78-79 1.4094162536137433E-5 9.865913775296202E-5 0.0 5.700968945486034 5.637665014454973E-5 80-81 1.4094162536137433E-5 1.1275330028909946E-4 0.0 6.154638896280494 6.342373141261844E-5 82-83 1.4094162536137433E-5 1.1275330028909946E-4 0.0 6.622121126360369 7.047081268068716E-5 84-85 1.4094162536137433E-5 1.1275330028909946E-4 0.0 7.064424135069434 7.047081268068716E-5 86-87 1.4094162536137433E-5 1.1980038155716818E-4 0.0 7.530933867934314 8.456497521682458E-5 88 1.4094162536137433E-5 1.268474628252369E-4 0.0 7.905007035805938 9.865913775296202E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12600 0.0 40.819183 1 GTATCAA 21270 0.0 37.764236 1 GTACTAG 6825 0.0 37.50688 1 GCATCGT 6340 0.0 37.44184 8 TACCTGG 4120 0.0 33.806477 2 CAACGCA 23190 0.0 32.371006 5 TAGGCAT 8480 0.0 31.87308 5 ATCAACG 23655 0.0 31.734447 3 GGGCACC 7000 0.0 30.077812 94 TATCAAC 26775 0.0 29.559835 2 TCTAACG 3090 0.0 29.542603 2 ACCTGGG 4635 0.0 29.511562 3 CTAGGCA 9500 0.0 29.242184 4 CTAACGC 3075 0.0 29.196932 3 CTGTGCG 8200 0.0 29.006464 9 TAACGCC 3150 0.0 28.949236 4 AACGCAG 27185 0.0 28.75551 6 CATCGTC 8855 0.0 27.657143 9 GTACATG 31010 0.0 27.587147 1 CGCCTAC 3330 0.0 27.526344 7 >>END_MODULE