FastQCFastQC Report
Mon 6 Jun 2016
ERR1211243_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211243_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7095136
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176160.248282767236597No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148070.20869226467258697No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92250.13001864939586782No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134250.041.1539761
GTACTAG69850.036.3509371
GTATCAA225550.036.315631
GGGCACC72350.032.424394
TACCTGG41300.032.2069852
TAGGCAT83950.031.4544685
CAACGCA249100.031.3301625
ATCAACG252950.030.736063
GCATCGT77500.030.6167778
CTGTGCG83700.030.5943229
TATCAAC284450.028.6851732
ACCTGGG45250.028.6706713
TAGTACT94450.028.5167524
CTAGGCA96750.028.373264
AACGCAG287150.027.996776
GAACCGT8800.027.7643456
CTAACGC29100.027.1371143
AACCGTG10000.026.7819127
ACTGTGC102650.026.685758
TAACGCC29650.026.6337284
Produced by FastQC (version 0.11.5)