FastQCFastQC Report
Mon 6 Jun 2016
ERR1211242_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211242_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11657234
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287050.24624194727497103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224280.19239555455436513No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127870.10969154432346473No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA203150.044.923791
GTATCAA357250.038.9051061
CAACGCA385050.033.6580165
GGGCACC121100.033.56842494
GTACTAG122400.032.9929581
ATCAACG393450.032.9394343
TATCAAC441350.031.2353232
GTGGTAT63300.031.1548521
AACGCAG449000.029.7436186
GCATCGT121700.029.6252848
CTGTGCG155600.029.45479
TAGGCAT146800.028.9795385
TAGTACT166250.028.1904754
CGCCTAC57750.027.757047
TCTAACG59000.027.6813492
TAACGCC59050.027.543884
ACTGTGC176700.027.4803038
CTAACGC59000.027.4078753
TCAACGC480750.027.3588184
ACGCAGA471600.027.3116267
Produced by FastQC (version 0.11.5)