##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211242_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11657234 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.563227863488027 31.0 31.0 34.0 30.0 34.0 2 31.590936666451064 33.0 31.0 34.0 30.0 34.0 3 31.65890416199932 33.0 31.0 34.0 28.0 34.0 4 35.2543564794187 37.0 35.0 37.0 32.0 37.0 5 35.316571152299076 37.0 35.0 37.0 33.0 37.0 6 35.37403967356236 37.0 35.0 37.0 33.0 37.0 7 35.29210111077808 37.0 35.0 37.0 32.0 37.0 8 35.301458991043674 37.0 35.0 37.0 32.0 37.0 9 36.87905209760737 39.0 37.0 39.0 33.0 39.0 10-11 36.83946002971202 39.0 37.0 39.0 32.5 39.0 12-13 36.730149150304435 39.0 37.0 39.0 32.0 39.0 14-15 37.943905689806 40.0 38.0 41.0 32.5 41.0 16-17 37.925371275896154 40.0 38.0 41.0 32.5 41.0 18-19 37.94113307668011 40.0 38.0 41.0 32.5 41.0 20-21 37.91048982974864 40.0 38.0 41.0 32.0 41.0 22-23 37.79432543774964 40.0 37.0 41.0 32.0 41.0 24-25 37.70043408239039 40.0 37.0 41.0 32.0 41.0 26-27 37.55474214552097 40.0 37.0 41.0 32.0 41.0 28-29 37.445029669988614 40.0 37.0 41.0 31.0 41.0 30-31 37.31151609378348 40.0 36.5 41.0 31.0 41.0 32-33 37.14983044005122 39.5 36.0 41.0 31.0 41.0 34-35 37.0163571821583 39.0 36.0 41.0 30.5 41.0 36-37 36.91738100135933 39.0 36.0 41.0 30.0 41.0 38-39 36.77805944360386 39.0 35.0 41.0 30.0 41.0 40-41 36.61509364056688 39.0 35.0 41.0 30.0 41.0 42-43 36.4046268179913 39.0 35.0 40.0 29.5 41.0 44-45 36.166717850907 38.5 35.0 40.0 28.5 41.0 46-47 35.932814207898716 38.0 35.0 40.0 28.0 41.0 48-49 35.719199168516305 38.0 34.5 40.0 27.0 41.0 50-51 35.0151695934044 37.5 33.5 39.5 26.5 40.5 52-53 35.101173314355705 38.0 33.5 39.5 26.5 40.5 54-55 35.57441237775616 38.0 34.0 40.0 28.0 41.0 56-57 35.511362129301 38.0 34.0 40.0 27.5 41.0 58-59 35.27290324617315 37.5 34.0 40.0 27.0 41.0 60-61 35.05333722390749 37.0 34.0 40.0 27.0 41.0 62-63 34.75061892898435 36.5 34.0 40.0 26.0 41.0 64-65 34.49973248370925 36.0 33.0 39.5 26.0 41.0 66-67 34.33735189668492 36.0 33.0 39.0 26.0 41.0 68-69 34.05583142621998 35.0 33.0 39.0 26.0 41.0 70-71 33.78901680278529 35.0 33.0 38.5 26.0 41.0 72-73 33.48473295637713 35.0 33.0 37.5 25.5 40.0 74-75 33.17085348033676 35.0 33.0 37.0 25.0 39.5 76-77 32.870331461133915 35.0 32.0 36.5 25.0 39.0 78-79 32.5494131369414 35.0 32.0 36.0 24.0 39.0 80-81 32.26988031637694 35.0 32.0 36.0 24.0 38.0 82-83 31.984603208617074 35.0 31.5 35.0 23.0 37.0 84-85 31.754878601561913 35.0 31.0 35.0 23.0 37.0 86-87 31.540270616511602 35.0 31.0 35.0 23.0 36.5 88-89 31.347840576932747 34.0 31.0 35.0 21.5 36.0 90-91 31.12430984914603 34.0 31.0 35.0 20.0 36.0 92-93 30.929984677325685 34.0 30.5 35.0 20.0 35.5 94-95 30.778806962269094 34.0 30.0 35.0 20.0 35.0 96-97 30.599302973587044 34.0 30.0 35.0 19.0 35.0 98-99 30.370900592713504 34.0 29.5 35.0 18.5 35.0 100 29.187663643022006 33.0 27.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.47598088310930464 1101 2 -0.4098872437951542 1101 3 -0.4791674133217221 1101 4 -0.3658553452363833 1101 5 -0.29860588326718585 1101 6 -0.2958228610498068 1101 7 -0.34276822075752733 1101 8 -0.3339525213326553 1101 9 -0.5467396749008557 1101 10-11 -0.5029199183868585 1101 12-13 -0.5297766747049693 1101 14-15 -0.7022471960131185 1101 16-17 -0.6615855954392984 1101 18-19 -0.6549924554070046 1101 20-21 -0.650132821917019 1101 22-23 -0.8051217792703866 1101 24-25 -0.8584203490802267 1101 26-27 -0.8524572654469011 1101 28-29 -0.8233496060031413 1101 30-31 -0.8890022574799161 1101 32-33 -0.9249676796197335 1101 34-35 -0.8966453737028672 1101 36-37 -0.9854175896221875 1101 38-39 -1.0044423121367245 1101 40-41 -0.9753304822724687 1101 42-43 -1.0921139393539576 1101 44-45 -1.0610532437075904 1101 46-47 -1.2513026480170097 1101 48-49 -1.2292444142226557 1101 50-51 -1.185193499298272 1101 52-53 -1.1269644110734092 1101 54-55 -1.0228336109432945 1101 56-57 -0.9474593822664872 1101 58-59 -0.9090622480200565 1101 60-61 -0.9166400398896215 1101 62-63 -1.0382608079196558 1101 64-65 -0.9733518694053132 1101 66-67 -0.9552401507074535 1101 68-69 -1.003573828779679 1101 70-71 -1.066067685533234 1101 72-73 -1.0977723477112278 1101 74-75 -1.099524746941178 1101 76-77 -1.1005635098560518 1101 78-79 -1.1248303017267673 1101 80-81 -1.203109348778355 1101 82-83 -1.1307454227914668 1101 84-85 -1.2382590505658833 1101 86-87 -1.238787262185923 1101 88-89 -1.368916209767196 1101 90-91 -1.4910400747090264 1101 92-93 -1.5824046362703683 1101 94-95 -1.5641822543381494 1101 96-97 -1.6564281289543459 1101 98-99 -1.7309412760929312 1101 100 -1.8334344082249707 1102 1 -0.5292213148702523 1102 2 -0.4727325928867998 1102 3 -0.5026343240386915 1102 4 -0.30034725582216026 1102 5 -0.371066645495425 1102 6 -0.393381148709274 1102 7 -0.4238430734226526 1102 8 -0.42649963209968433 1102 9 -0.5714020807266635 1102 10-11 -0.6385625800045815 1102 12-13 -0.6138176193001144 1102 14-15 -0.7734081895826108 1102 16-17 -0.8095158225160546 1102 18-19 -0.8045361274285838 1102 20-21 -0.8583500453976001 1102 22-23 -0.8191336231028359 1102 24-25 -0.8604820904302599 1102 26-27 -0.9623431053041713 1102 28-29 -0.9992679262726156 1102 30-31 -0.9532089810475455 1102 32-33 -1.0441066561828336 1102 34-35 -1.0898085312337358 1102 36-37 -0.9929287077609246 1102 38-39 -1.0839300164142003 1102 40-41 -1.1772876990859231 1102 42-43 -1.1786425040319628 1102 44-45 -1.3356024203230277 1102 46-47 -1.273662251211661 1102 48-49 -1.2278946764010357 1102 50-51 -1.2891786427131962 1102 52-53 -1.1413594659060067 1102 54-55 -1.0093571672965354 1102 56-57 -0.9759924017295987 1102 58-59 -0.9918101549624154 1102 60-61 -0.9457449096469404 1102 62-63 -1.0530951464545737 1102 64-65 -1.105403995684462 1102 66-67 -1.15728053537417 1102 68-69 -1.1192502161597915 1102 70-71 -1.1319105569301726 1102 72-73 -1.2014925861120531 1102 74-75 -1.2395045721768838 1102 76-77 -1.326934997953689 1102 78-79 -1.2724396651721115 1102 80-81 -1.2524163631938876 1102 82-83 -1.275624735854663 1102 84-85 -1.309843755256118 1102 86-87 -1.41091710990775 1102 88-89 -1.4864303329007917 1102 90-91 -1.4828675496713934 1102 92-93 -1.5046323687906913 1102 94-95 -1.5903102511474216 1102 96-97 -1.5932661944130402 1102 98-99 -1.7028713840651086 1102 100 -1.789777902182923 1103 1 -0.5535712999099509 1103 2 -0.47416217517942627 1103 3 -0.5665291732564022 1103 4 -0.5148092790642096 1103 5 -0.46027916763315346 1103 6 -0.49089339765549767 1103 7 -0.5013834536294937 1103 8 -0.4720558971420985 1103 9 -0.7263274145139533 1103 10-11 -0.6574509499169707 1103 12-13 -0.6869633666862924 1103 14-15 -0.8514083469267746 1103 16-17 -0.8641148180914584 1103 18-19 -0.7925270191868066 1103 20-21 -0.8393633442636457 1103 22-23 -0.8241889877574238 1103 24-25 -0.8991975554115399 1103 26-27 -0.9446999549666089 1103 28-29 -0.9796204933047434 1103 30-31 -0.9688969011779278 1103 32-33 -1.0352749118799096 1103 34-35 -1.0578403200447042 1103 36-37 -1.0249816765302313 1103 38-39 -1.09733826612716 1103 40-41 -1.109717047439645 1103 42-43 -1.234957340140852 1103 44-45 -1.269793785335267 1103 46-47 -1.2529623417120703 1103 48-49 -1.3315571164719486 1103 50-51 -1.3805299632429566 1103 52-53 -1.3071663502580222 1103 54-55 -1.0668110529586414 1103 56-57 -0.9791638222068002 1103 58-59 -0.9605573874132531 1103 60-61 -1.0175945030129512 1103 62-63 -0.9807100822609272 1103 64-65 -0.9241666021261352 1103 66-67 -0.9240481458998318 1103 68-69 -0.998335970963268 1103 70-71 -1.0456781414981293 1103 72-73 -1.0920459904807132 1103 74-75 -1.1005036275082674 1103 76-77 -1.1186226331885258 1103 78-79 -1.1604403283541274 1103 80-81 -1.1887273690324882 1103 82-83 -1.114832686098822 1103 84-85 -1.1452708161006164 1103 86-87 -1.2204448242471777 1103 88-89 -1.3070460654062082 1103 90-91 -1.3355502595830977 1103 92-93 -1.3810613607320121 1103 94-95 -1.4205112647903775 1103 96-97 -1.4089574870917119 1103 98-99 -1.5210801965950331 1103 100 -1.6054247098933025 1104 1 -0.5513927956880593 1104 2 -0.5023067995449146 1104 3 -0.5633353875183538 1104 4 -0.4273528198015981 1104 5 -0.39071928301631687 1104 6 -0.3868627098813562 1104 7 -0.4405038367900005 1104 8 -0.3913059983219682 1104 9 -0.49152647219697343 1104 10-11 -0.5215893654918702 1104 12-13 -0.5605858303841487 1104 14-15 -0.7830380256817904 1104 16-17 -0.8144521042703516 1104 18-19 -0.8036639767866589 1104 20-21 -0.7501123717799203 1104 22-23 -0.7179651264517233 1104 24-25 -0.7822551301792231 1104 26-27 -0.8046246440210965 1104 28-29 -0.8501173399133677 1104 30-31 -0.8838908069420484 1104 32-33 -0.9614370366011045 1104 34-35 -1.0681847976007148 1104 36-37 -0.9828546574951886 1104 38-39 -0.9815876854097141 1104 40-41 -0.9495145413093127 1104 42-43 -0.9983867452666573 1104 44-45 -1.0438703600930381 1104 46-47 -1.0404572911518244 1104 48-49 -1.0967019495725552 1104 50-51 -1.1360231116169999 1104 52-53 -0.9770429604076512 1104 54-55 -0.8201049644302572 1104 56-57 -0.8963274828920049 1104 58-59 -0.8965912407223797 1104 60-61 -0.8518854989729832 1104 62-63 -0.8848298341224918 1104 64-65 -0.8390566210114727 1104 66-67 -0.9304571204848173 1104 68-69 -0.9479938349309762 1104 70-71 -1.0093111856516828 1104 72-73 -0.9950944526513226 1104 74-75 -0.9352309247466195 1104 76-77 -0.8687521565728318 1104 78-79 -0.9884549806964635 1104 80-81 -1.0255414693871288 1104 82-83 -1.0154930234496007 1104 84-85 -1.0726666934334474 1104 86-87 -1.0483058852490394 1104 88-89 -1.1234062990910516 1104 90-91 -1.156003917956287 1104 92-93 -1.2098352710742724 1104 94-95 -1.21588970531905 1104 96-97 -1.3286645589824744 1104 98-99 -1.2681476291388059 1104 100 -1.2427740844603932 1105 1 -0.5693982992246553 1105 2 -0.5549845386723753 1105 3 -0.5397730210907419 1105 4 -0.3490061507719773 1105 5 -0.32647313691339264 1105 6 -0.38770071232641357 1105 7 -0.36521844845818663 1105 8 -0.4613489839616278 1105 9 -0.6225454744240579 1105 10-11 -0.593676700598202 1105 12-13 -0.6402684804699916 1105 14-15 -0.8487372216284186 1105 16-17 -0.8291744174313109 1105 18-19 -0.8045675584101915 1105 20-21 -0.765385360957417 1105 22-23 -0.8124917731011294 1105 24-25 -0.8350986331122385 1105 26-27 -0.8118476370256502 1105 28-29 -0.8571691423582948 1105 30-31 -0.9404233364927279 1105 32-33 -0.9365203264475142 1105 34-35 -0.9272394667045418 1105 36-37 -0.8482377381454427 1105 38-39 -0.8953853171609083 1105 40-41 -0.9378410227305025 1105 42-43 -1.0079272954432312 1105 44-45 -1.0100439692940597 1105 46-47 -1.116362258201093 1105 48-49 -1.0786107674598426 1105 50-51 -1.0788572844479418 1105 52-53 -0.9300335262934922 1105 54-55 -0.8282910292771462 1105 56-57 -0.7450674584840229 1105 58-59 -0.7131845912335777 1105 60-61 -0.7183047148167603 1105 62-63 -0.7544945779190186 1105 64-65 -0.7428150825934168 1105 66-67 -0.740844892341137 1105 68-69 -0.7851832331209394 1105 70-71 -0.8086579535623386 1105 72-73 -0.7380159391647823 1105 74-75 -0.7867098993148289 1105 76-77 -0.7706478796150904 1105 78-79 -0.847466856325191 1105 80-81 -0.7968020695391473 1105 82-83 -0.8904373284185496 1105 84-85 -0.9391147922699048 1105 86-87 -0.9574105991875079 1105 88-89 -1.0298991700229543 1105 90-91 -1.0215497845693022 1105 92-93 -1.0283286851980193 1105 94-95 -1.165169879085795 1105 96-97 -1.1319080236858206 1105 98-99 -1.1243604616521274 1105 100 -1.2032180617458579 1106 1 -0.5772826750163276 1106 2 -0.5752478298880774 1106 3 -0.6201531118449388 1106 4 -0.46979000558330597 1106 5 -0.38949136732762213 1106 6 -0.4753509644276761 1106 7 -0.4546704289498251 1106 8 -0.4920779205736636 1106 9 -0.5925146168590985 1106 10-11 -0.5891133606130623 1106 12-13 -0.6379145964602557 1106 14-15 -0.9204857424514756 1106 16-17 -0.8558653383980044 1106 18-19 -0.9328729600015109 1106 20-21 -0.8424452248883867 1106 22-23 -0.8934728281340085 1106 24-25 -0.7962813220854699 1106 26-27 -0.9058359546203292 1106 28-29 -0.9236693717418305 1106 30-31 -1.0125308497854206 1106 32-33 -0.9709261657184172 1106 34-35 -0.9555428752551762 1106 36-37 -0.9834224763469237 1106 38-39 -1.0362341887346105 1106 40-41 -1.0147452676914313 1106 42-43 -0.962303554362208 1106 44-45 -1.0698269038746204 1106 46-47 -1.0060680486241154 1106 48-49 -1.0282045990062798 1106 50-51 -1.0010713947739518 1106 52-53 -0.9277010558266525 1106 54-55 -0.8647653775687729 1106 56-57 -0.8193753083039681 1106 58-59 -0.781520881768607 1106 60-61 -0.7770143001559475 1106 62-63 -0.8442931954989277 1106 64-65 -0.9058116494153623 1106 66-67 -0.9099399062534417 1106 68-69 -0.9145436837707237 1106 70-71 -0.8574313363054316 1106 72-73 -0.8658945652914838 1106 74-75 -0.8266752802226591 1106 76-77 -0.8355951797460861 1106 78-79 -0.8308752216342015 1106 80-81 -0.7992606678574141 1106 82-83 -0.8005497104179184 1106 84-85 -0.7468417080416536 1106 86-87 -0.8585624859988776 1106 88-89 -0.9036220244043456 1106 90-91 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1.3486721073685146 2310 96-97 1.4152204996672566 2310 98-99 1.4900978006663408 2310 100 1.569699851964696 2311 1 0.7476633838279554 2311 2 0.7093129630118824 2311 3 0.7299703264122321 2311 4 0.6237656047497566 2311 5 0.5855992582411318 2311 6 0.6366543630978825 2311 7 0.6769084617698979 2311 8 0.5798746442707667 2311 9 0.8154952370811301 2311 10-11 0.8401872521896578 2311 12-13 0.9088509934303417 2311 14-15 1.0992081559339155 2311 16-17 1.1103575350077506 2311 18-19 1.0047948020393065 2311 20-21 1.0629671008875619 2311 22-23 1.0872110433274216 2311 24-25 1.1273984563751114 2311 26-27 1.165317184892416 2311 28-29 1.1781180247009573 2311 30-31 1.1671113338128336 2311 32-33 1.218140433265667 2311 34-35 1.2500863787933625 2311 36-37 1.2866069715051367 2311 38-39 1.2876936664444898 2311 40-41 1.3006225831076392 2311 42-43 1.3476051647291385 2311 44-45 1.4033927232123986 2311 46-47 1.4280934621400903 2311 48-49 1.4288106166988328 2311 50-51 1.3515163916316268 2311 52-53 1.305330910504587 2311 54-55 1.2148408039660268 2311 56-57 1.1616671216143644 2311 58-59 1.156123170655171 2311 60-61 1.1528186367038487 2311 62-63 1.1752577648654565 2311 64-65 1.1496894163293874 2311 66-67 1.1614274829036404 2311 68-69 1.1687172209980048 2311 70-71 1.1181777124785484 2311 72-73 1.1376581710501341 2311 74-75 1.1772435285444516 2311 76-77 1.2005444401064054 2311 78-79 1.2187836421619593 2311 80-81 1.1682331120997134 2311 82-83 1.2431368582332922 2311 84-85 1.214338459825285 2311 86-87 1.2192209489172185 2311 88-89 1.2832802876275373 2311 90-91 1.335332890904585 2311 92-93 1.3677791776055983 2311 94-95 1.427174976769905 2311 96-97 1.4508748840403598 2311 98-99 1.5533723068976535 2311 100 1.6794925506001555 2312 1 0.6129703161163746 2312 2 0.5756379504912985 2312 3 0.6618008942916127 2312 4 0.33752555074290314 2312 5 0.4666230489669587 2312 6 0.5368854135986467 2312 7 0.4568806367872895 2312 8 0.5747192432039867 2312 9 0.7315232853562392 2312 10-11 0.707238573113024 2312 12-13 0.8169054520915608 2312 14-15 1.041039317830034 2312 16-17 1.0437822848675964 2312 18-19 1.0104556969544447 2312 20-21 1.0540263342011826 2312 22-23 1.1139393658733852 2312 24-25 1.1184206329457353 2312 26-27 1.1369849835622148 2312 28-29 1.1760365496299983 2312 30-31 1.178752250131275 2312 32-33 1.1827699988690696 2312 34-35 1.1981471252844003 2312 36-37 1.2507002111763654 2312 38-39 1.2210153440124856 2312 40-41 1.3084791642493911 2312 42-43 1.3430504894760986 2312 44-45 1.383838677397577 2312 46-47 1.3924302139509734 2312 48-49 1.4123495068713936 2312 50-51 1.3907064258732973 2312 52-53 1.2952614924220782 2312 54-55 1.1888306945258762 2312 56-57 1.1067718046172814 2312 58-59 1.1303249740389063 2312 60-61 1.0685710723026673 2312 62-63 1.077387308417947 2312 64-65 1.1107767840385137 2312 66-67 1.0640234698784568 2312 68-69 1.0622588153551575 2312 70-71 1.0570778299283177 2312 72-73 1.117847892165969 2312 74-75 1.1327473377586585 2312 76-77 1.088697893305671 2312 78-79 1.077149687437391 2312 80-81 1.1212313125823812 2312 82-83 1.133355629830767 2312 84-85 1.1460449853396355 2312 86-87 1.1624840064363227 2312 88-89 1.0826535856921673 2312 90-91 1.2012068776046938 2312 92-93 1.3566893606344905 2312 94-95 1.4015576928866977 2312 96-97 1.4085884612675414 2312 98-99 1.0590736466968806 2312 100 1.3199965350736669 2313 1 0.6336151922332149 2313 2 0.6582610269833111 2313 3 0.630204359253856 2313 4 0.4810739598217424 2313 5 0.3808210103911378 2313 6 0.5493550469731048 2313 7 0.5537089192416644 2313 8 0.5599625943261657 2313 9 0.7233375303102818 2313 10-11 0.7063733712301499 2313 12-13 0.8231943722103736 2313 14-15 1.0053748242319074 2313 16-17 1.025084158016547 2313 18-19 0.9851880435259659 2313 20-21 1.010729487931016 2313 22-23 1.0509406287743772 2313 24-25 1.017632907176889 2313 26-27 1.062957328114905 2313 28-29 1.086195349437567 2313 30-31 1.137555547612763 2313 32-33 1.1195846984011055 2313 34-35 1.1829075599560284 2313 36-37 1.2061174716594678 2313 38-39 1.2216435387518914 2313 40-41 1.1994657874413264 2313 42-43 1.294237775841573 2313 44-45 1.276988313483841 2313 46-47 1.3382812399706268 2313 48-49 1.3069519804273355 2313 50-51 1.3277290572418465 2313 52-53 1.237364672078975 2313 54-55 1.14277001027218 2313 56-57 1.0685110967922213 2313 58-59 1.0694445234210974 2313 60-61 1.0733205321511932 2313 62-63 1.0807375757478468 2313 64-65 1.0912545739125932 2313 66-67 1.0241650856720241 2313 68-69 1.0915803290677104 2313 70-71 1.1217690479035056 2313 72-73 1.1081512581984825 2313 74-75 1.1044878068640358 2313 76-77 0.8895775395084726 2313 78-79 1.0798522391276961 2313 80-81 1.1274963425907245 2313 82-83 1.1527504915313926 2313 84-85 1.1588536950855257 2313 86-87 1.1531666532162603 2313 88-89 1.239057630544952 2313 90-91 1.2463765540714071 2313 92-93 1.3534281285860743 2313 94-95 1.356084581451647 2313 96-97 1.39504847234738 2313 98-99 1.4254178010567493 2313 100 1.50268744648913 2314 1 0.6093276116433515 2314 2 0.5614303971921828 2314 3 0.6282804902444603 2314 4 0.5188702918651913 2314 5 0.4950464785595514 2314 6 0.5127642844172868 2314 7 0.569639860266868 2314 8 0.5739855589717564 2314 9 0.698947320131488 2314 10-11 0.700580308613759 2314 12-13 0.7537368066025181 2314 14-15 0.9541891910099736 2314 16-17 0.9716610964592647 2314 18-19 0.9150436758239238 2314 20-21 0.9274570117847247 2314 22-23 0.9952886958308582 2314 24-25 0.9899718119439953 2314 26-27 1.043115196912602 2314 28-29 1.0392217903511352 2314 30-31 1.0663835524622414 2314 32-33 1.0559442818360125 2314 34-35 1.0659860959846483 2314 36-37 1.0929421975054083 2314 38-39 1.0953852919753757 2314 40-41 1.152325250831467 2314 42-43 1.2212883293930545 2314 44-45 1.2581865980783533 2314 46-47 1.2831126468758498 2314 48-49 1.2731818207152301 2314 50-51 1.2875543829296632 2314 52-53 1.2223477966778091 2314 54-55 1.0907582077833737 2314 56-57 1.0366062524550443 2314 58-59 1.0145997628332353 2314 60-61 1.071447496422941 2314 62-63 1.0849740752520631 2314 64-65 1.094004536868347 2314 66-67 1.1271961840837577 2314 68-69 1.076576734288274 2314 70-71 1.0916736849155768 2314 72-73 1.109838098910572 2314 74-75 1.1433108221594495 2314 76-77 1.1880101350834096 2314 78-79 1.171311477475193 2314 80-81 1.1425832068953525 2314 82-83 1.123444831918448 2314 84-85 1.2154532650849816 2314 86-87 1.2435215286452106 2314 88-89 1.2898138184404253 2314 90-91 1.3688077544520674 2314 92-93 1.3837427953388755 2314 94-95 1.3731713320007657 2314 96-97 1.3946893172064634 2314 98-99 1.4821365254292793 2314 100 1.5511265303888848 2315 1 0.49436472177947977 2315 2 0.5181769740062379 2315 3 0.5845919916454427 2315 4 0.2881693777808252 2315 5 0.3471641789609521 2315 6 0.42773139723536246 2315 7 0.4852883874930711 2315 8 0.4989140280545499 2315 9 0.6372268070944145 2315 10-11 0.5175410818144499 2315 12-13 0.5764528366403141 2315 14-15 0.7835738769481324 2315 16-17 0.8069122622168905 2315 18-19 0.6786568687773737 2315 20-21 0.8157980279312227 2315 22-23 0.6665956112517577 2315 24-25 0.8071978163032725 2315 26-27 0.8942234630861492 2315 28-29 0.924648875490341 2315 30-31 0.9326512055221059 2315 32-33 0.9311794151850563 2315 34-35 0.9580949874517657 2315 36-37 1.062687231089015 2315 38-39 1.059725407224981 2315 40-41 1.0590304686247833 2315 42-43 1.12595013743978 2315 44-45 1.0973906712288155 2315 46-47 1.1326583273863946 2315 48-49 1.1947920294477115 2315 50-51 1.095949134316193 2315 52-53 1.1432673301376823 2315 54-55 0.9971065118597764 2315 56-57 1.0010660180736792 2315 58-59 0.9572442656581686 2315 60-61 0.9477447583187057 2315 62-63 1.0059445434665477 2315 64-65 1.0251288947200052 2315 66-67 0.9376608312892785 2315 68-69 0.9891570445050135 2315 70-71 0.9798060537854454 2315 72-73 0.6930645618678568 2315 74-75 0.9114994378157988 2315 76-77 0.9931298743567325 2315 78-79 0.9841943216126268 2315 80-81 0.700072245736159 2315 82-83 0.7070819216065871 2315 84-85 0.9990815013866836 2315 86-87 1.1292640388284525 2315 88-89 1.1913996303515297 2315 90-91 1.1994840531586597 2315 92-93 1.23790390548686 2315 94-95 1.3140192271174023 2315 96-97 1.154707257353131 2315 98-99 0.6666000731849628 2315 100 1.142339748087359 2316 1 0.3735514099895312 2316 2 0.33430928502146173 2316 3 0.36601671481174236 2316 4 0.24424900108130743 2316 5 0.15425937978658055 2316 6 0.23501806099798728 2316 7 0.1966430033249722 2316 8 0.2515501345097917 2316 9 0.34626742159183266 2316 10-11 0.3146836265335864 2316 12-13 0.16996192375059138 2316 14-15 0.13272889534856347 2316 16-17 0.324179987164662 2316 18-19 0.36020247401798855 2316 20-21 0.3837845115611813 2316 22-23 0.3495894238155657 2316 24-25 0.4501203074339699 2316 26-27 0.5057125364624397 2316 28-29 0.46613340253187374 2316 30-31 0.343138898948979 2316 32-33 0.5648056792011289 2316 34-35 0.5475620341852689 2316 36-37 0.5758553626159113 2316 38-39 0.7351000968174333 2316 40-41 0.7237129574894254 2316 42-43 0.7021903248815349 2316 44-45 0.6288758594230472 2316 46-47 0.4652573527056987 2316 48-49 0.4185663969615163 2316 50-51 0.49150648272725306 2316 52-53 0.6431605546395929 2316 54-55 0.33333954409398103 2316 56-57 0.17450705112906206 2316 58-59 0.22252801134826683 2316 60-61 0.3224953204308605 2316 62-63 0.41026664057935136 2316 64-65 0.36222530534255526 2316 66-67 0.3364829520288666 2316 68-69 0.24638807117653272 2316 70-71 0.038502523084467555 2316 72-73 0.02199754017740929 2316 74-75 0.263657894541133 2316 76-77 0.4328476561345198 2316 78-79 0.46133494243698436 2316 80-81 0.6252868589446265 2316 82-83 0.6619105334890492 2316 84-85 0.6849814738154976 2316 86-87 0.7263359050672733 2316 88-89 0.6846923140450905 2316 90-91 0.554226878990125 2316 92-93 0.7030243534108571 2316 94-95 0.8391553722887934 2316 96-97 0.7517616020528592 2316 98-99 0.6307855426636024 2316 100 0.527305698392766 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 37.0 1 710.0 2 251.0 3 268.0 4 189.0 5 163.0 6 184.0 7 690.0 8 2451.0 9 4636.0 10 7095.0 11 10451.0 12 14024.0 13 17982.0 14 21359.0 15 25888.0 16 31974.0 17 39820.0 18 49771.0 19 61415.0 20 76592.0 21 96179.0 22 105641.0 23 105526.0 24 104418.0 25 109898.0 26 124400.0 27 144731.0 28 172020.0 29 208203.0 30 256354.0 31 320995.0 32 411304.0 33 546325.0 34 789727.0 35 1116384.0 36 1719378.0 37 2365992.0 38 2011826.0 39 581609.0 40 374.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.37858355368772 20.533183366177273 12.83824258612318 23.24999049401183 2 15.466293384226738 19.49472181069471 41.5883418643867 23.45064294069185 3 19.30501254715479 26.12098154717126 28.40058438991176 26.17342151576219 4 12.438182395993705 16.195251309795342 39.587779110128515 31.77878718408244 5 13.155185248359752 38.38732699622513 34.574276950870996 13.88321080454413 6 30.121935435363785 37.961678072186245 17.853935307650342 14.06245118479963 7 27.493191701497828 31.91800206137288 22.716275919085966 17.87253031804333 8 26.213521155530493 35.53709432606857 19.562483451623383 18.686901066777555 9 26.234813199706743 15.529101080792222 21.61843612397748 36.61764959552356 10-11 25.98408087337519 26.19917045885049 26.83513226870275 20.98161639907157 12-13 25.851716099067623 23.768196288418437 27.69213242371022 22.687955188803723 14-15 23.247196791133273 24.964374977933442 25.532809490247622 26.25561874068566 16-17 21.831816077606913 27.890138464823227 26.69146304114855 23.586582416421308 18-19 21.199664561970923 27.999062736589515 29.045530547374188 21.755742154065373 20-21 22.893259969468332 27.01106258168319 28.465392408816843 21.63028504003163 22-23 23.188339453334436 26.827120540804195 28.03671974919758 21.94782025666379 24-25 22.525997345371856 26.72103142831573 28.95256909365188 21.800402132660533 26-27 22.481306090202153 27.043082543379025 27.86338291406117 22.61222845235765 28-29 21.6781828397374 27.396368491970126 28.523992073705223 22.401456594587252 30-31 22.58115828682777 27.085476934985643 28.48304837132176 21.850316406864827 32-33 22.74787744814909 27.239168377622995 27.666623950854536 22.346330223373382 34-35 22.760098787228294 27.153256888751454 28.35854224399876 21.7281020800215 36-37 22.388732617213122 26.738596159906834 27.9825383384239 22.890132884456136 38-39 22.570935187700446 26.957876732956414 28.475612696371982 21.99557538297116 40-41 22.400707304771718 26.02348198300004 29.007878753244913 22.567931958983326 42-43 22.319320460972097 26.222604156485573 28.447652032467026 23.010423350075303 44-45 22.48848782083618 27.00274757857623 28.27267880844204 22.236085792145545 46-47 22.45399838701801 26.92107622648725 27.63052138866129 22.99440399783345 48-49 23.093600175000276 26.84077901211863 27.684409588182092 22.381211224698998 50-51 22.77085409961267 26.61953810832229 27.711029410092724 22.898578381972314 52-53 22.280638828910636 27.142123217356534 27.485535743149587 23.09170221058324 54-55 22.180434513223606 27.049734539277097 27.51436374726807 23.25546720023122 56-57 23.049605266133256 26.6930364654206 27.144982274317048 23.112375994129096 58-59 22.110224143388056 27.12511663049605 27.886660560801307 22.87799866531459 60-61 22.189259463232716 27.655168469801083 27.316146851107675 22.839425215858522 62-63 22.592558826576767 26.962718195100464 26.948873493015764 23.495849485307005 64-65 21.95159339159404 27.08480622902793 27.38268242337392 23.58091795600411 66-67 22.419079205648078 26.793172023057398 27.30663897833488 23.48110979295964 68-69 22.038434677747812 26.77851393487487 27.593474854681823 23.589576532695496 70-71 22.012108476654536 26.47677776902429 27.64399101377232 23.86712274054885 72-73 22.576016030187393 26.487227617950403 27.834046056922684 23.10271029493952 74-75 22.759337854880872 26.740276959516983 27.442882441079647 23.057502744522495 76-77 22.486153652903646 26.72218647658204 27.11429264945608 23.677367221058237 78-79 22.437894940234724 26.75141365094662 27.096460827557234 23.71423058126142 80-81 22.051048465714917 27.16025389305676 27.621241511117507 23.167456130110818 82-83 22.93244604583827 26.32609419926319 28.54572436686642 22.19573538803212 84-85 23.03089592299763 26.560798987966876 27.474841805478757 22.933463283556733 86-87 21.946449249054623 27.18618006722382 27.842164175727056 23.025206507994504 88-89 22.930336120012086 26.015412318031267 27.755908940409142 23.29834262154751 90-91 22.618307378288023 26.279686080390185 27.711431422729337 23.39057511859245 92-93 22.906364527596963 26.335017368597804 27.75883826792103 22.99977983588421 94-95 21.98653474120599 26.348687059829757 28.378296111192313 23.28648208777194 96-97 22.357469999882447 26.235813155424694 27.90744777217063 23.49926907252223 98-99 21.87996620753262 26.564550181217705 27.778601762224575 23.776881849025102 100 22.742024668747106 26.014404060568037 27.065645460898363 24.177925809786498 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 613.0 1 461.5 2 497.5 3 736.0 4 677.0 5 598.0 6 854.5 7 1551.0 8 2613.5 9 3279.5 10 3242.5 11 3264.0 12 3790.5 13 4525.5 14 5396.5 15 6503.5 16 7668.5 17 8777.5 18 9712.5 19 10691.5 20 11825.5 21 13167.0 22 14887.0 23 17024.5 24 19713.0 25 23432.0 26 28839.0 27 35329.0 28 44823.5 29 63167.5 30 90000.5 31 118560.0 32 156884.0 33 200195.0 34 236854.5 35 271500.5 36 306734.0 37 357941.0 38 381082.0 39 372463.5 40 359059.0 41 339116.0 42 336241.0 43 352288.0 44 378849.0 45 432565.5 46 488132.5 47 526418.5 48 551845.5 49 545241.0 50 523097.5 51 531449.0 52 530500.0 53 497408.5 54 462940.0 55 392648.0 56 333845.0 57 273549.5 58 212556.0 59 198806.5 60 163512.0 61 111576.5 62 81913.5 63 68660.5 64 50574.0 65 24552.5 66 13102.5 67 8988.0 68 6836.0 69 5348.0 70 4111.5 71 3122.0 72 2383.5 73 1792.5 74 1331.0 75 890.0 76 630.5 77 446.5 78 294.0 79 205.0 80 153.5 81 130.5 82 110.0 83 86.0 84 73.0 85 60.0 86 41.0 87 32.5 88 31.0 89 30.0 90 22.5 91 14.5 92 10.5 93 8.0 94 7.5 95 6.5 96 4.0 97 3.0 98 5.5 99 5.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05732920862702079 2 0.13422566622579593 3 0.017173885331631843 4 0.016968004588395497 5 0.019610140793261937 6 0.0527569404543136 7 0.057869645578016195 8 0.049660150941466905 9 0.04085874916811312 10-11 0.039867948091288206 12-13 0.041081786639952496 14-15 0.018473507523311276 16-17 0.017461260535732576 18-19 0.01737976607486819 20-21 0.021016992538710297 22-23 0.025396247514633406 24-25 0.024628483909647864 26-27 0.0191726442138847 28-29 0.018044589308235555 30-31 0.016371808269440246 32-33 0.023071510788923 34-35 0.023084378335375272 36-37 0.02292996777794801 38-39 0.020931208895695157 40-41 0.02446549498791909 42-43 0.03162842917968362 44-45 0.04110752173285704 46-47 0.047378306037264076 48-49 0.040155323295388935 50-51 0.027660935690233205 52-53 0.03972211589816246 54-55 0.03676686939629075 56-57 0.03868413381767922 58-59 0.04332073972264776 60-61 0.022882786774289683 62-63 0.025361934057427343 64-65 0.024838653835034967 66-67 0.03181715319431694 68-69 0.038842833557257234 70-71 0.04062713333197224 72-73 0.04476190492530217 74-75 0.02669586970631283 76-77 0.020202047930066427 78-79 0.01978170807929222 80-81 0.024015130862089583 82-83 0.02047655558771489 84-85 0.012773184444954953 86-87 0.014518881580313135 88-89 0.018366277969542346 90-91 0.02061380941653912 92-93 0.01966161097907102 94-95 0.02336317517517449 96-97 0.024559856995235746 98-99 0.02708618528203174 100 0.028291445466394515 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.1657234E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.29407062653949 #Duplication Level Percentage of deduplicated Percentage of total 1 74.77301371770166 47.32688411207415 2 14.859561316811742 18.810442469313532 3 5.202508463991534 9.87863814465149 4 2.2184066806572793 5.6164795649563555 5 1.105359038674516 3.49813365307743 6 0.5933637272680018 2.253384339655657 7 0.3698553515702681 1.63867555207245 8 0.22149775216163212 1.1215595495150468 9 0.1465055334971405 0.8345638425912171 >10 0.47437416521759773 4.6911094490898995 >50 0.020029831035673327 0.8834213100241936 >100 0.013885252085902832 1.6956406520189173 >500 0.0011890274404557624 0.5238444615611073 >1k 3.6011355827738066E-4 0.3339067749958233 >5k 3.601133144084214E-5 0.16485064412177275 >10k+ 5.401699716126322E-5 0.7284654802809343 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28705 0.24624194727497103 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22428 0.19239555455436513 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12787 0.10969154432346473 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.578364301514407E-6 0.0 10-11 0.0 0.0 0.0 1.7156728603028814E-5 0.0 12-13 0.0 0.0 0.0 1.7156728603028814E-5 0.0 14-15 0.0 0.0 0.0 2.5735092904543223E-5 0.0 16-17 0.0 0.0 0.0 4.718100365832924E-5 0.0 18-19 0.0 0.0 0.0 9.436200731665848E-5 0.0 20-21 0.0 0.0 0.0 1.9301319678407416E-4 0.0 22-23 0.0 0.0 0.0 3.9460475786966276E-4 0.0 24-25 0.0 0.0 0.0 7.720527871362966E-4 0.0 26-27 0.0 0.0 0.0 0.0015226596635188072 0.0 28-29 8.578364301514407E-6 0.0 0.0 0.004529376351199607 0.0 30-31 8.578364301514407E-6 0.0 0.0 0.01766714127896892 0.0 32-33 8.578364301514407E-6 0.0 0.0 0.05118281060498571 0.0 34-35 8.578364301514407E-6 0.0 0.0 0.10176513570886542 0.0 36-37 8.578364301514407E-6 0.0 0.0 0.1870340768659186 0.0 38-39 8.578364301514407E-6 0.0 0.0 0.3108799222868821 0.0 40-41 8.578364301514407E-6 0.0 0.0 0.4410737572909663 0.0 42-43 8.578364301514407E-6 0.0 0.0 0.5698950540068082 0.0 44-45 8.578364301514407E-6 0.0 0.0 0.7195317517002746 0.0 46-47 8.578364301514407E-6 0.0 0.0 0.8989139276092424 0.0 48-49 8.578364301514407E-6 0.0 0.0 1.097112745613582 0.0 50-51 8.578364301514407E-6 0.0 0.0 1.3020970497804196 0.0 52-53 8.578364301514407E-6 0.0 0.0 1.4934203087970954 0.0 54-55 8.578364301514407E-6 0.0 0.0 1.7009052061578245 0.0 56-57 8.578364301514407E-6 0.0 0.0 1.943535662061858 0.0 58-59 8.578364301514407E-6 0.0 0.0 2.2215475815274877 0.0 60-61 8.578364301514407E-6 0.0 0.0 2.5041360583479753 0.0 62-63 8.578364301514407E-6 0.0 0.0 2.7903874967252094 0.0 64-65 8.578364301514407E-6 0.0 0.0 3.0797228570688384 0.0 66-67 8.578364301514407E-6 0.0 0.0 3.4011112756250754 0.0 68-69 8.578364301514407E-6 0.0 0.0 3.749877543849596 0.0 70-71 8.578364301514407E-6 0.0 0.0 4.127488562037959 0.0 72-73 8.578364301514407E-6 0.0 0.0 4.5071112066550265 0.0 74-75 8.578364301514407E-6 0.0 0.0 4.874368139131461 0.0 76-77 8.578364301514407E-6 0.0 0.0 5.285670683114022 0.0 78-79 8.578364301514407E-6 0.0 0.0 5.725938074160646 0.0 80-81 1.286754645227161E-5 4.289182150757204E-6 0.0 6.1922450900445165 0.0 82-83 1.7156728603028814E-5 8.578364301514407E-6 0.0 6.663484665401759 4.289182150757204E-6 84-85 1.7156728603028814E-5 8.578364301514407E-6 0.0 7.1163236493322515 8.578364301514407E-6 86-87 1.7156728603028814E-5 8.578364301514407E-6 0.0 7.594893436985137 8.578364301514407E-6 88 1.7156728603028814E-5 8.578364301514407E-6 0.0 7.976883710149423 8.578364301514407E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 20315 0.0 44.92379 1 GTATCAA 35725 0.0 38.905106 1 CAACGCA 38505 0.0 33.658016 5 GGGCACC 12110 0.0 33.568424 94 GTACTAG 12240 0.0 32.992958 1 ATCAACG 39345 0.0 32.939434 3 TATCAAC 44135 0.0 31.235323 2 GTGGTAT 6330 0.0 31.154852 1 AACGCAG 44900 0.0 29.743618 6 GCATCGT 12170 0.0 29.625284 8 CTGTGCG 15560 0.0 29.4547 9 TAGGCAT 14680 0.0 28.979538 5 TAGTACT 16625 0.0 28.190475 4 CGCCTAC 5775 0.0 27.75704 7 TCTAACG 5900 0.0 27.681349 2 TAACGCC 5905 0.0 27.54388 4 ACTGTGC 17670 0.0 27.480303 8 CTAACGC 5900 0.0 27.407875 3 TCAACGC 48075 0.0 27.358818 4 ACGCAGA 47160 0.0 27.311626 7 >>END_MODULE