FastQCFastQC Report
Mon 6 Jun 2016
ERR1211242_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211242_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11657234
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268610.23042344350297847No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225140.19313329388429537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129770.11132143354075247No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA212800.044.829341
GTATCAA361100.037.0170061
GGGCACC122600.035.77408694
GTACTAG116450.033.9149931
CAACGCA398800.031.776475
ATCAACG401700.031.5668873
CTGTGCG159800.030.5501279
TATCAAC454700.029.3515472
TAGGCAT146300.028.7768675
AACGCAG462550.028.321356
TAGTACT176050.028.1648884
GTGGTAT66400.027.8984491
ACTGTGC183750.027.872348
GTACTGT190000.026.832186
TACCTGG57900.026.7026842
GCATCGT139450.026.2478188
CTAACGC58050.026.2321823
CTAGGCA167100.026.1013624
CTAGTAC199850.026.0101363
TCAACGC496650.025.9861374
Produced by FastQC (version 0.11.5)