##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211242_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11657234 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.187012716738806 33.0 31.0 34.0 30.0 34.0 2 32.32982789913971 34.0 31.0 34.0 31.0 34.0 3 32.41093101502466 34.0 31.0 34.0 31.0 34.0 4 36.01573374953269 37.0 35.0 37.0 35.0 37.0 5 35.903810972654405 37.0 35.0 37.0 35.0 37.0 6 35.93038545850585 37.0 35.0 37.0 35.0 37.0 7 35.871268261407465 37.0 35.0 37.0 35.0 37.0 8 35.86160507715638 37.0 35.0 37.0 35.0 37.0 9 37.47119162230079 39.0 37.0 39.0 35.0 39.0 10-11 37.45124315081948 39.0 37.0 39.0 35.0 39.0 12-13 37.348201425827085 39.0 37.0 39.0 34.0 39.0 14-15 38.58265618584991 40.0 38.0 41.0 34.0 41.0 16-17 38.54052556549864 40.0 38.0 41.0 34.0 41.0 18-19 38.5646666696405 40.0 38.0 41.0 34.0 41.0 20-21 38.52829496259576 40.0 38.0 41.0 34.0 41.0 22-23 38.39048628516851 40.0 38.0 41.0 34.0 41.0 24-25 38.323831022007454 40.0 38.0 41.0 34.0 41.0 26-27 38.187094511442425 40.0 38.0 41.0 33.5 41.0 28-29 38.17998647878218 40.0 38.0 41.0 33.5 41.0 30-31 38.206705810314865 40.0 38.0 41.0 34.0 41.0 32-33 38.09010147690267 40.0 37.0 41.0 33.5 41.0 34-35 37.959462253224046 40.0 37.0 41.0 33.0 41.0 36-37 37.83233342489308 40.0 37.0 41.0 33.0 41.0 38-39 37.740434051508274 40.0 37.0 41.0 33.0 41.0 40-41 37.63918687743593 40.0 37.0 41.0 33.0 41.0 42-43 37.49117071854267 40.0 36.0 41.0 32.0 41.0 44-45 37.331644925374235 40.0 36.0 41.0 32.0 41.0 46-47 37.09134315224349 39.0 36.0 41.0 31.0 41.0 48-49 36.84125985632612 39.0 35.0 41.0 31.0 41.0 50-51 36.62515370284237 39.0 35.0 41.0 30.5 41.0 52-53 36.4026030531771 39.0 35.0 40.0 30.0 41.0 54-55 36.1498553173077 38.0 35.0 40.0 30.0 41.0 56-57 35.87118200595441 38.0 35.0 40.0 29.0 41.0 58-59 35.57556985645137 37.5 34.0 40.0 29.0 41.0 60-61 35.31138849061449 37.0 34.0 40.0 28.0 41.0 62-63 34.99868622350722 36.5 34.0 39.5 28.0 41.0 64-65 34.6245177886967 36.0 34.0 39.0 27.5 41.0 66-67 34.33161168421257 35.5 33.0 39.0 27.0 40.0 68-69 34.01609305432146 35.0 33.0 38.5 26.0 40.0 70-71 33.7383277628295 35.0 33.0 38.0 26.0 40.0 72-73 33.42996739192162 35.0 33.0 37.0 26.0 40.0 74-75 33.11751226748987 35.0 32.0 36.5 26.0 39.0 76-77 32.13697331631157 34.0 31.0 35.5 24.5 38.0 78-79 32.645238484532435 35.0 32.0 36.0 26.0 38.0 80-81 32.59143699954895 35.0 32.0 35.5 26.0 37.0 82-83 32.35692866764106 35.0 32.0 35.0 25.0 37.0 84-85 32.12909773450546 34.5 32.0 35.0 25.0 36.5 86-87 31.915981569899 34.0 32.0 35.0 25.0 36.0 88-89 31.714682273685163 34.0 31.0 35.0 25.0 36.0 90-91 31.51379105026115 34.0 31.0 35.0 24.0 35.5 92-93 31.33843654506721 34.0 31.0 35.0 24.0 35.0 94-95 31.171114262611525 34.0 31.0 35.0 23.5 35.0 96-97 31.01479428996621 34.0 31.0 35.0 23.0 35.0 98-99 30.85675748638142 34.0 30.0 35.0 23.0 35.0 100 29.00865162353265 32.0 27.0 34.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3375921397101962 1101 2 -0.29175876546742074 1101 3 -0.3794244316717901 1101 4 -0.16988236569390125 1101 5 -0.26737209517981597 1101 6 -0.3524576833761657 1101 7 -0.36103731694112895 1101 8 -0.3053342515038935 1101 9 -0.4328649796325763 1101 10-11 -0.44410720073749843 1101 12-13 -0.4453217652335155 1101 14-15 -0.7084317135050426 1101 16-17 -0.603446808701456 1101 18-19 -0.6633708691417368 1101 20-21 -0.7084986143687573 1101 22-23 -0.7324275356824828 1101 24-25 -0.6968863923720434 1101 26-27 -0.829724032871269 1101 28-29 -0.858807213750076 1101 30-31 -0.7051464459745063 1101 32-33 -0.6912446396473797 1101 34-35 -0.6902017035761574 1101 36-37 -0.658677497239232 1101 38-39 -0.7419612469333998 1101 40-41 -0.7672440891147616 1101 42-43 -0.7563481140638615 1101 44-45 -0.8567609577900157 1101 46-47 -0.7877252994216022 1101 48-49 -0.8027160488511882 1101 50-51 -0.8222542613026178 1101 52-53 -0.8570735074700266 1101 54-55 -0.7418437582498925 1101 56-57 -0.7569258774861893 1101 58-59 -0.7566251585693351 1101 60-61 -0.8293295505181462 1101 62-63 -0.8676622533707246 1101 64-65 -0.8224502142649968 1101 66-67 -0.9717686596879602 1101 68-69 -0.9674246643978321 1101 70-71 -0.9877334214467766 1101 72-73 -1.0445797150807508 1101 74-75 -1.1032316464133878 1101 76-77 -1.0323059930242167 1101 78-79 -0.8603871237192138 1101 80-81 -0.8073848166626547 1101 82-83 -0.7658036523272429 1101 84-85 -0.800383020821279 1101 86-87 -0.9691901430570375 1101 88-89 -0.9941755736511659 1101 90-91 -0.9735705306497842 1101 92-93 -1.0972218370721158 1101 94-95 -1.050551122820572 1101 96-97 -1.0213451796102433 1101 98-99 -1.2133297883054475 1101 100 -1.0450827769858684 1102 1 -0.40629551751151993 1102 2 -0.3322115408437156 1102 3 -0.3628692373088569 1102 4 -0.23788138365154055 1102 5 -0.2724647286528139 1102 6 -0.2982202641396796 1102 7 -0.3168099303260661 1102 8 -0.33333398135097525 1102 9 -0.48108567813758896 1102 10-11 -0.5124029533814749 1102 12-13 -0.47357664584029635 1102 14-15 -0.638045241959972 1102 16-17 -0.6462760284774731 1102 18-19 -0.6129632211674121 1102 20-21 -0.7021781997753038 1102 22-23 -0.6715954989234305 1102 24-25 -0.7412545026455533 1102 26-27 -0.8123108634185385 1102 28-29 -0.8074633284810986 1102 30-31 -0.7084955777774127 1102 32-33 -0.6485156434092119 1102 34-35 -0.7141825094852052 1102 36-37 -0.8388932225576013 1102 38-39 -0.7973192075344286 1102 40-41 -0.7560405008268987 1102 42-43 -0.7421422119071295 1102 44-45 -0.8212861606157631 1102 46-47 -0.8840674996573838 1102 48-49 -0.861417836309748 1102 50-51 -0.8498844703311619 1102 52-53 -0.9153068055055158 1102 54-55 -0.8655864006329139 1102 56-57 -0.9468527331275567 1102 58-59 -0.9475193966685111 1102 60-61 -0.9398622514563542 1102 62-63 -0.9781858275211945 1102 64-65 -0.9376487572646042 1102 66-67 -0.9970937843883974 1102 68-69 -1.0157854020521953 1102 70-71 -1.0336737920354295 1102 72-73 -1.0766507908529448 1102 74-75 -1.169217523165699 1102 76-77 -1.0240364958668664 1102 78-79 -0.8093072878279841 1102 80-81 -0.7761320714960327 1102 82-83 -0.7781423277976103 1102 84-85 -0.8624953177302892 1102 86-87 -0.8505222228260756 1102 88-89 -0.970078572325729 1102 90-91 -0.9828664491731232 1102 92-93 -1.1066759342354366 1102 94-95 -1.063381047640938 1102 96-97 -1.1639087984545995 1102 98-99 -1.158094012575262 1102 100 -1.0856215723365885 1103 1 -0.37141331008554346 1103 2 -0.36357886761724245 1103 3 -0.37735286296995696 1103 4 -0.23438385843972753 1103 5 -0.22756398437339698 1103 6 -0.2948389893089711 1103 7 -0.38912911930375316 1103 8 -0.3774965290522516 1103 9 -0.5277786845927821 1103 10-11 -0.5134283536503119 1103 12-13 -0.5529229817099548 1103 14-15 -0.6948627954958724 1103 16-17 -0.7465364914733996 1103 18-19 -0.7173734632316879 1103 20-21 -0.7204799469168819 1103 22-23 -0.7350281740887397 1103 24-25 -0.8455726676611164 1103 26-27 -0.8413978140163039 1103 28-29 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-1.040304956338833 1103 94-95 -1.1033040917529107 1103 96-97 -1.0699909899863655 1103 98-99 -1.0980529589240646 1103 100 -1.1887186156512968 1104 1 -0.3952724720969911 1104 2 -0.35070035220298834 1104 3 -0.4452598929247955 1104 4 -0.25258859400153 1104 5 -0.30079446843177493 1104 6 -0.3632377341861357 1104 7 -0.3852388133232836 1104 8 -0.37233061296174697 1104 9 -0.5467710511883368 1104 10-11 -0.5451512523052315 1104 12-13 -0.5487513951954881 1104 14-15 -0.7114406038418011 1104 16-17 -0.6750227762465357 1104 18-19 -0.6878862702516173 1104 20-21 -0.6972412853192225 1104 22-23 -0.7154229811649273 1104 24-25 -0.7432746619046426 1104 26-27 -0.8347611931654484 1104 28-29 -0.874269446454214 1104 30-31 -0.8078550650082832 1104 32-33 -0.7810923766180835 1104 34-35 -0.8435234907642553 1104 36-37 -0.8293505267487618 1104 38-39 -0.825597755987701 1104 40-41 -0.8140281939720566 1104 42-43 -0.7763655687489788 1104 44-45 -0.8641342780231227 1104 46-47 -0.9713125309065234 1104 48-49 -0.9041019114015612 1104 50-51 -0.8583749255646893 1104 52-53 -0.8289245494083062 1104 54-55 -0.8927834891924107 1104 56-57 -0.8974035805510638 1104 58-59 -0.8863106187711054 1104 60-61 -0.95977303130271 1104 62-63 -1.0198860513480739 1104 64-65 -0.9474822658179747 1104 66-67 -0.9438625131375247 1104 68-69 -1.0284348361464666 1104 70-71 -1.015932719141162 1104 72-73 -1.038840129966026 1104 74-75 -1.0019884037910955 1104 76-77 -0.8826945998160873 1104 78-79 -0.7038069696940283 1104 80-81 -0.7360569709453415 1104 82-83 -0.7669738184924668 1104 84-85 -0.7872269285119415 1104 86-87 -0.9099880798798772 1104 88-89 -0.9070798810981167 1104 90-91 -0.8657146977223071 1104 92-93 -0.9222811554745682 1104 94-95 -0.9483812299740855 1104 96-97 -0.9535263693422174 1104 98-99 -1.0727281056070446 1104 100 -1.0160846559709071 1105 1 -0.39594653909032473 1105 2 -0.3941492692340347 1105 3 -0.4638156768205022 1105 4 -0.25422959778560994 1105 5 -0.2993958318972787 1105 6 -0.4013254846578036 1105 7 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1105 70-71 -0.9707376159208465 1105 72-73 -0.9772708146443847 1105 74-75 -0.9968705998721035 1105 76-77 -1.0109223013190274 1105 78-79 -0.9413684349722686 1105 80-81 -0.8722414017520137 1105 82-83 -0.8492162389017466 1105 84-85 -0.823116248017719 1105 86-87 -0.8865868168297304 1105 88-89 -0.8716382417316062 1105 90-91 -0.8206308450427819 1105 92-93 -0.9150053276257388 1105 94-95 -0.9656309403507635 1105 96-97 -1.0198988451321256 1105 98-99 -1.1073471648387816 1105 100 -1.0914843601474224 1106 1 -0.41714106814706753 1106 2 -0.42686972662240663 1106 3 -0.4570995954924797 1106 4 -0.3134153556002275 1106 5 -0.32323978127335806 1106 6 -0.3679435485881797 1106 7 -0.3870085797336458 1106 8 -0.3880231316976861 1106 9 -0.547291024028425 1106 10-11 -0.5468334041084333 1106 12-13 -0.6517488868011156 1106 14-15 -0.732281607421875 1106 16-17 -0.7198435543394623 1106 18-19 -0.6780196837670118 1106 20-21 -0.7230879077957013 1106 22-23 -0.7031207795210506 1106 24-25 -0.7298195008497075 1106 26-27 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2311 26-27 0.3603488579037162 2311 28-29 0.6558046919183553 2311 30-31 0.8732409358565718 2311 32-33 0.9501483376098037 2311 34-35 1.005650804921963 2311 36-37 1.043353693718906 2311 38-39 0.9863217664891408 2311 40-41 0.9880237768668181 2311 42-43 0.9777588712945473 2311 44-45 0.994939679830793 2311 46-47 0.6370230106941861 2311 48-49 0.896172408505393 2311 50-51 1.0147383099775027 2311 52-53 0.9416408855202434 2311 54-55 0.9758067158546595 2311 56-57 1.0708568048143476 2311 58-59 1.1035200140725436 2311 60-61 1.153185363932316 2311 62-63 1.1030217778932396 2311 64-65 1.1623147654798842 2311 66-67 1.1345280946922713 2311 68-69 1.153104988338555 2311 70-71 1.1495077510384135 2311 72-73 1.1765662220425668 2311 74-75 1.1666270618613694 2311 76-77 1.2038279418758364 2311 78-79 0.9300931319121517 2311 80-81 0.846695169390351 2311 82-83 0.9881577198239597 2311 84-85 1.0519314212101136 2311 86-87 1.0407795363579808 2311 88-89 1.043701014760341 2311 90-91 1.1672094839106357 2311 92-93 1.2130032491121945 2311 94-95 1.2373907292068793 2311 96-97 1.2379636723327465 2311 98-99 1.3105836094423928 2311 100 1.4471949297800073 2312 1 0.24297288763088432 2312 2 0.3529819710694966 2312 3 0.45483641202570624 2312 4 0.17078641712641485 2312 5 0.3149832041574925 2312 6 0.40699584463204985 2312 7 0.3226177864680082 2312 8 0.4165946731701382 2312 9 0.607974637000261 2312 10-11 0.6394662069568753 2312 12-13 0.6747389433671387 2312 14-15 0.8943691041990505 2312 16-17 0.8297164952733027 2312 18-19 0.8491224731392393 2312 20-21 0.8630419958533366 2312 22-23 0.9087909138063637 2312 24-25 0.9243816940225429 2312 26-27 0.9730864606073055 2312 28-29 0.9806879593977058 2312 30-31 0.9972094478062274 2312 32-33 0.8546190726476368 2312 34-35 0.9780545106343439 2312 36-37 1.0329964964534994 2312 38-39 0.8326545627097133 2312 40-41 0.9447957420860362 2312 42-43 1.0729480816971133 2312 44-45 0.8367517578122659 2312 46-47 0.8950166337422587 2312 48-49 1.0772359250453718 2312 50-51 0.879632813092698 2312 52-53 1.0682058839855273 2312 54-55 0.978992052889545 2312 56-57 0.9881608270199891 2312 58-59 1.1197151520251296 2312 60-61 1.1177986916595941 2312 62-63 1.1315171224165894 2312 64-65 1.1838776989431778 2312 66-67 1.1200774573792955 2312 68-69 1.1813006867848799 2312 70-71 1.07603026389463 2312 72-73 1.0889073399968439 2312 74-75 1.1486625246324138 2312 76-77 1.107348818562322 2312 78-79 1.0358236052444738 2312 80-81 0.997404968425819 2312 82-83 1.0118448749381486 2312 84-85 1.077370564475686 2312 86-87 1.089592798503162 2312 88-89 1.078781200271198 2312 90-91 1.1348765151916815 2312 92-93 1.187492870880451 2312 94-95 1.2471343089217442 2312 96-97 0.9014147859063542 2312 98-99 0.6759588309743236 2312 100 0.9075280048809873 2313 1 0.028410183658564847 2313 2 0.20852775729039053 2313 3 0.28557277973346373 2313 4 0.17527434658124008 2313 5 0.32156906410379804 2313 6 0.24029041361851 2313 7 0.23779621396352013 2313 8 0.3802249027935076 2313 9 0.5517144802578855 2313 10-11 0.32944645392363014 2313 12-13 0.403611274971567 2313 14-15 0.45440550580652683 2313 16-17 0.6883583623060119 2313 18-19 0.6493365638975419 2313 20-21 0.6187438493924162 2313 22-23 0.6068067225097664 2313 24-25 0.5218453977427728 2313 26-27 0.6991701498857239 2313 28-29 0.5825303595865066 2313 30-31 0.7782592543637463 2313 32-33 0.8751682409538546 2313 34-35 0.9252109071361119 2313 36-37 0.9589991334697672 2313 38-39 0.9586300544037414 2313 40-41 0.9694336397580585 2313 42-43 1.049358906385315 2313 44-45 0.8557577270486547 2313 46-47 0.9024422526310687 2313 48-49 0.977188653519498 2313 50-51 0.9152543568897613 2313 52-53 0.9579890012445347 2313 54-55 0.9056289349507836 2313 56-57 0.6072760145057501 2313 58-59 0.7244372711513947 2313 60-61 0.5968962149821593 2313 62-63 0.9463572851603672 2313 64-65 1.035166029505156 2313 66-67 1.010832045758285 2313 68-69 1.1051235081412116 2313 70-71 1.0308359877642417 2313 72-73 1.0708916641436232 2313 74-75 1.155599160390814 2313 76-77 1.1216604957115521 2313 78-79 0.9441720182662507 2313 80-81 0.8836679513185928 2313 82-83 0.9608655651656335 2313 84-85 0.9191341257716701 2313 86-87 0.9496589653216176 2313 88-89 1.0121633488634636 2313 90-91 0.8690316504953763 2313 92-93 1.049688925374955 2313 94-95 1.1558585013188036 2313 96-97 1.228988426653359 2313 98-99 1.2723274866974528 2313 100 1.2469474671185843 2314 1 -0.3863014106798346 2314 2 0.03986840197227792 2314 3 0.28574490326243307 2314 4 0.19616561193057436 2314 5 0.3186940152839952 2314 6 0.3748704453889218 2314 7 0.4375122373099103 2314 8 0.4712323094799089 2314 9 0.47492340751279727 2314 10-11 0.591421762974754 2314 12-13 0.48002322994784663 2314 14-15 0.7773134909986439 2314 16-17 0.8190123549967581 2314 18-19 0.8570783061027143 2314 20-21 0.8407562370140624 2314 22-23 0.9141316237395429 2314 24-25 0.8456114127818566 2314 26-27 0.7607577056703931 2314 28-29 0.8202674525787614 2314 30-31 0.8813533718407882 2314 32-33 0.9492557481836812 2314 34-35 0.9825447688177817 2314 36-37 0.934412347493101 2314 38-39 0.689844575950417 2314 40-41 0.7164975705567471 2314 42-43 0.9182190048780896 2314 44-45 0.9867095021063648 2314 46-47 0.9144412472958052 2314 48-49 0.9748616045250813 2314 50-51 0.9507202774155701 2314 52-53 0.9688605849115177 2314 54-55 0.884177134289466 2314 56-57 0.8714024211258788 2314 58-59 1.005300403102801 2314 60-61 1.0538553527713788 2314 62-63 0.9718919691247692 2314 64-65 1.0767477422466598 2314 66-67 0.8480217242732024 2314 68-69 0.7844153690348392 2314 70-71 0.9124791487078028 2314 72-73 1.0381494170219767 2314 74-75 1.1083511575386211 2314 76-77 0.9368894543297159 2314 78-79 0.858209617688928 2314 80-81 0.9228421081895632 2314 82-83 0.939870973512555 2314 84-85 0.9189165301686018 2314 86-87 1.0042809493306208 2314 88-89 0.9868117879140037 2314 90-91 1.1052574560781956 2314 92-93 0.9909903688464574 2314 94-95 0.9908491192333884 2314 96-97 1.0180343315084102 2314 98-99 1.1446660831884365 2314 100 1.1750571724463086 2315 1 0.40371182124332705 2315 2 0.4345957102612701 2315 3 0.4295420503234766 2315 4 0.31033632744719597 2315 5 0.3553281429772426 2315 6 0.4093306777551291 2315 7 0.3628796713688516 2315 8 0.27436096289094536 2315 9 0.503425940544453 2315 10-11 0.5560982328221229 2315 12-13 0.3567975189727548 2315 14-15 0.5971459656646303 2315 16-17 0.7665019568981251 2315 18-19 0.7591624451312526 2315 20-21 0.5602950311457846 2315 22-23 0.864943838597533 2315 24-25 0.846550649656983 2315 26-27 0.8315438641600821 2315 28-29 0.7708865784124228 2315 30-31 0.8701042923707263 2315 32-33 0.8277875640687995 2315 34-35 0.8729539212190147 2315 36-37 0.8742950753140732 2315 38-39 0.9146598402258661 2315 40-41 0.9292993636082869 2315 42-43 0.9780932719732718 2315 44-45 0.98681026031187 2315 46-47 0.9718543470479162 2315 48-49 0.8976328478368814 2315 50-51 0.9450999513738694 2315 52-53 0.971193396672966 2315 54-55 0.7773298177916388 2315 56-57 0.7719148062355998 2315 58-59 0.7751159674302457 2315 60-61 0.9159641368124625 2315 62-63 1.0069158177029678 2315 64-65 1.0437565608122483 2315 66-67 0.9945479554866026 2315 68-69 1.0085976532233545 2315 70-71 0.8732258633235261 2315 72-73 0.9903002898399293 2315 74-75 0.4015362355447323 2315 76-77 0.4782377324757121 2315 78-79 0.7689210854544513 2315 80-81 0.8045327123387267 2315 82-83 0.7723706438257736 2315 84-85 0.801440426903298 2315 86-87 0.8339410533782896 2315 88-89 0.8201103003969088 2315 90-91 0.883013748650388 2315 92-93 0.9564806834639548 2315 94-95 0.9992339985383936 2315 96-97 1.087576848507652 2315 98-99 1.1520818130260544 2315 100 1.059167825700282 2316 1 0.2812153827981021 2316 2 0.11364945979121899 2316 3 0.22620116436298332 2316 4 0.028468751103247314 2316 5 0.2716646303861552 2316 6 0.3481504103370625 2316 7 0.4114732361228306 2316 8 0.4247064399910343 2316 9 0.6013123598063217 2316 10-11 0.5963918494756015 2316 12-13 0.40926222705233073 2316 14-15 0.47456143970845943 2316 16-17 0.7589111926784113 2316 18-19 0.7161107826995377 2316 20-21 0.7065730459193418 2316 22-23 0.763765305795296 2316 24-25 0.5008317390883761 2316 26-27 0.7131178662066802 2316 28-29 0.8776184050191418 2316 30-31 0.5659147964126205 2316 32-33 0.6712545101403151 2316 34-35 0.8238506600221385 2316 36-37 0.8516178192181982 2316 38-39 0.7759703229573347 2316 40-41 0.7914460406972026 2316 42-43 0.767566023547829 2316 44-45 0.6357147190488561 2316 46-47 0.8058615233673478 2316 48-49 0.900013311893268 2316 50-51 0.8820968730760512 2316 52-53 0.8375801124939244 2316 54-55 0.7609908667782292 2316 56-57 0.9263147740978894 2316 58-59 0.7116060384919152 2316 60-61 0.8815530170448156 2316 62-63 0.8982975632969712 2316 64-65 0.9140134437823946 2316 66-67 0.8913535605127976 2316 68-69 0.8959285563076378 2316 70-71 0.8591500780177341 2316 72-73 0.8889427775768368 2316 74-75 0.8820608049450485 2316 76-77 0.7707620611079093 2316 78-79 0.5256962873839868 2316 80-81 0.4966347966970446 2316 82-83 0.49575704740264825 2316 84-85 0.6208844742590287 2316 86-87 0.653202621928969 2316 88-89 0.6332198729216536 2316 90-91 0.4864195519959189 2316 92-93 0.29184797132294804 2316 94-95 0.413413558013481 2316 96-97 0.6535514408530787 2316 98-99 0.8139778559974076 2316 100 0.4111472202379538 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 9.0 12 47.0 13 204.0 14 849.0 15 2615.0 16 6012.0 17 12003.0 18 20949.0 19 31839.0 20 46837.0 21 67187.0 22 82515.0 23 82676.0 24 83194.0 25 91290.0 26 110230.0 27 136468.0 28 169506.0 29 210047.0 30 260254.0 31 327918.0 32 419027.0 33 557718.0 34 808390.0 35 1147581.0 36 1843381.0 37 2673484.0 38 2114973.0 39 350002.0 40 28.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.719126236730915 21.32199060606734 13.574763930861932 23.384119226339813 2 15.184571228474955 19.908899486790776 41.32353352433347 23.582995760400795 3 18.871852448016398 26.206920097855118 28.967720816104404 25.95350663802408 4 12.323114806372347 16.542032833791158 39.502251835569496 31.632600524267 5 12.972228231842992 38.607374613909265 34.672847778469574 13.747549375778165 6 30.191424483715434 38.190028612276286 17.617189463641203 14.001357440367071 7 27.339866847122796 32.052511738098076 22.69754803220382 17.910073382575312 8 26.247409977358267 35.876332241421935 19.36881424873173 18.507443532488065 9 26.045045759989016 15.529338083421093 21.61316240452817 36.81245375206172 10-11 25.99818643936867 26.359337295898833 26.769114841983416 20.87336142274908 12-13 25.782649278778248 23.790289685382458 27.78477534076912 22.64228569507018 14-15 23.141363169836513 25.09877248172259 25.512962160487756 26.246902187953143 16-17 21.75551945672001 27.98711370794351 26.69968547746934 23.557681357867143 18-19 21.163871292280827 27.995774984014215 29.086282389115638 21.75407133458932 20-21 22.83073423895987 26.975898698434754 28.587069993922658 21.60629706868272 22-23 23.064120528077243 26.813869396462316 28.160140733213385 21.961869342247052 24-25 22.403242844277695 26.711371040699916 29.079985423568356 21.805400691454036 26-27 22.397250893538093 27.0255914182255 27.993292025135265 22.58386566310115 28-29 21.60751668290625 27.41290341480747 28.622819178146003 22.35676072414028 30-31 22.519840716660077 27.024144281833955 28.610826850870165 21.845188150635806 32-33 22.7352703154583 27.15686861205684 27.846753067917923 22.261108004566942 34-35 22.72096484613513 27.107119358668406 28.493634769524462 21.678281025672003 36-37 22.365125936919817 26.713481622693465 28.10673205869562 22.8146603816911 38-39 22.47961762999928 26.869013134637758 28.70606914990373 21.945300085459234 40-41 22.24331621941564 25.98815149400817 29.247312030888693 22.5212202556875 42-43 22.290395477414997 26.164477195133973 28.612190532105103 22.932936795345928 44-45 22.384990222137727 26.94943394439325 28.40787712889637 22.25769870457266 46-47 22.364414892804664 26.89952825712535 27.74068950889904 22.995367341170947 48-49 23.077053076585543 26.79770709630873 27.84873424046922 22.27650558663651 50-51 22.70462001757816 26.56551824368012 27.83392725795057 22.895934480791148 52-53 22.199580506741267 27.046899922854973 27.59065570530049 23.16286386510327 54-55 22.135304789993665 27.019361458577084 27.55624723452929 23.289086516899964 56-57 23.065580446178274 26.63227955923127 27.19854434765049 23.10359564693997 58-59 21.963375628695324 27.149313134325336 27.95734952191445 22.929961715064888 60-61 22.164730292432036 27.633160767467075 27.30941586126135 22.89269307883954 62-63 22.58657248060029 26.940283223031862 26.896575832644015 23.57656846372383 64-65 21.835371792912913 27.093034396734 27.324845161642163 23.746748648710923 66-67 22.280773435089213 26.807925765475932 27.33247897765728 23.57882182177757 68-69 21.914658820385814 26.77599563715289 27.554669599608694 23.754675942852607 70-71 21.769065425850876 26.406181866355418 27.673630462916865 24.151122244876845 72-73 22.253233981463463 26.458020301921948 27.826832686047133 23.46191303056746 74-75 22.376576853578904 26.756598035118895 27.43635444097292 23.430470670329278 76-77 22.105124550570324 26.70365806656092 27.057486796156304 24.133730586712453 78-79 22.029327089741003 26.711759659274133 27.086304845978106 24.172608405006766 80-81 21.532962148898466 27.174379077880275 27.57160487112659 23.72105390209467 82-83 22.39207472402085 26.200726836339623 28.527214998903254 22.87998344073627 84-85 22.541538135916355 26.48575510722384 27.427552687111458 23.545154069748357 86-87 21.274792603958524 27.135158560695654 27.814984890530862 23.775063944814956 88-89 22.16249451936876 26.034598315603237 27.68845591812672 24.114451246901282 90-91 21.81685573222501 26.170543158597244 27.68089194245486 24.33170916672288 92-93 22.078577414367714 26.2463436224666 27.673486183537815 24.001592779627867 94-95 21.07269924040166 26.202382791354566 28.31588957831317 24.409028389930608 96-97 21.33463414466758 26.13474377998645 27.894969707667027 24.635652367678944 98-99 20.738486965022894 26.54742759583038 27.825519205544964 24.88856623360176 100 21.367612752221167 25.975551509486973 27.228473148381582 25.42836258991028 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 551.0 1 392.5 2 312.5 3 414.5 4 373.5 5 384.0 6 631.5 7 1222.5 8 2032.5 9 2530.5 10 2542.0 11 2648.5 12 3199.0 13 3952.5 14 4905.0 15 6101.5 16 7248.0 17 8473.0 18 9795.5 19 11033.5 20 12194.5 21 13432.5 22 15230.5 23 17536.5 24 20421.0 25 24307.0 26 30088.5 27 37243.5 28 47177.0 29 66216.0 30 93710.0 31 121562.0 32 159587.5 33 202829.5 34 237892.0 35 272662.0 36 307497.0 37 355931.5 38 378916.5 39 370716.5 40 354994.0 41 335189.5 42 334502.5 43 349690.5 44 378837.5 45 437586.0 46 491426.0 47 528416.5 48 557171.5 49 551777.0 50 527893.5 51 531075.5 52 529076.5 53 493847.5 54 459575.5 55 393362.5 56 334014.5 57 273934.0 58 212518.5 59 201807.5 60 165569.5 61 108634.0 62 78306.5 63 65279.5 64 46885.0 65 21078.0 66 10713.0 67 7633.5 68 5968.5 69 4694.5 70 3559.5 71 2606.5 72 1940.5 73 1388.0 74 1012.5 75 658.5 76 392.0 77 222.0 78 137.0 79 79.0 80 42.0 81 30.0 82 24.0 83 16.5 84 12.0 85 9.0 86 6.0 87 4.0 88 5.0 89 5.0 90 2.0 91 0.0 92 0.0 93 0.5 94 1.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04499352076144307 2 0.0 3 0.0 4 0.0311394624144973 5 0.0 6 0.0 7 9.178849802620416E-4 8 0.0 9 0.01122907887068236 10-11 4.760992187340496E-4 12-13 8.578364301514407E-6 14-15 1.243862823719589E-4 16-17 3.474237542113335E-4 18-19 0.0 20-21 4.289182150757204E-6 22-23 0.0 24-25 5.147018580908644E-4 26-27 5.533044974476792E-4 28-29 1.5012137527650214E-4 30-31 0.0023247367257104045 32-33 0.014853437788072197 34-35 0.02558068234711596 36-37 0.031070835500085183 38-39 0.0219091424260678 40-41 0.0013082005559809473 42-43 6.0048550110600856E-5 44-45 0.011254813963586902 46-47 0.018494953434065063 48-49 0.0034227673563042484 50-51 0.011096114224008885 52-53 0.01086020920571724 54-55 0.010435580172792277 56-57 4.718100365832924E-4 58-59 0.008166602815041715 60-61 0.004507930440445821 62-63 0.0014282976562021489 64-65 0.03598194906270218 66-67 0.06115515910549622 68-69 0.06042170895771673 70-71 0.05309578584422343 72-73 0.04616446748859978 74-75 0.03570744140505372 76-77 0.03186433419797526 78-79 0.01513652381002217 80-81 0.0011666575450059594 82-83 0.03896293065747844 84-85 0.05287274837238405 86-87 0.057976875131785115 88-89 0.07082726485545371 90-91 0.04906824380466241 92-93 0.03988081563774048 94-95 0.013163500020673858 96-97 0.0034871050885656066 98-99 0.02190056406176628 100 0.0314997537151609 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.1657234E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.4299276166325 #Duplication Level Percentage of deduplicated Percentage of total 1 73.95958068828156 46.172912689259086 2 15.267731015474558 19.063266843325835 3 5.348235513572192 10.016698679670458 4 2.322035723818248 5.798580886448324 5 1.169617211579031 3.6509558929023225 6 0.6248427867420443 2.3405333968848456 7 0.37691289242605786 1.6471451214354071 8 0.23711469254718393 1.184244247404861 9 0.16013305070363248 0.8997385288012487 >10 0.4981258385980283 4.899784551184518 >50 0.019295474458740965 0.8503866641856211 >100 0.014702063540967376 1.7868899813240715 >500 0.0012136059016179524 0.5387338165251288 >1k 3.8593161011090033E-4 0.36846119311927394 >5k 1.8377686518562207E-5 0.06629201318806478 >10k+ 5.513305955568662E-5 0.7153754943409857 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26861 0.23042344350297847 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22514 0.19313329388429537 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12977 0.11132143354075247 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 8.578364301514407E-6 0.0 5 0.0 0.0 0.0 8.578364301514407E-6 0.0 6 0.0 0.0 0.0 8.578364301514407E-6 0.0 7 0.0 0.0 0.0 8.578364301514407E-6 0.0 8 0.0 0.0 0.0 8.578364301514407E-6 0.0 9 0.0 0.0 0.0 3.431345720605763E-5 0.0 10-11 0.0 0.0 0.0 5.147018580908645E-5 0.0 12-13 0.0 0.0 0.0 6.862691441211526E-5 0.0 14-15 0.0 0.0 0.0 7.720527871362967E-5 0.0 16-17 0.0 0.0 0.0 9.436200731665847E-5 0.0 18-19 0.0 0.0 0.0 1.329646466734733E-4 0.0 20-21 0.0 0.0 0.0 2.144591075378602E-4 0.0 22-23 0.0 0.0 0.0 3.988939400204199E-4 0.0 24-25 0.0 0.0 0.0 7.977878800408399E-4 0.0 26-27 0.0 0.0 0.0 0.0015698406671771365 0.0 28-29 0.0 0.0 0.0 0.004619449176365508 0.0 30-31 0.0 0.0 0.0 0.017821551836396183 0.0 32-33 0.0 0.0 0.0 0.05149592090199098 0.0 34-35 0.0 0.0 0.0 0.10237419957427293 0.0 36-37 0.0 0.0 0.0 0.18772034601003978 0.0 38-39 0.0 0.0 0.0 0.31199939882822975 0.0 40-41 0.0 0.0 0.0 0.4428966597050381 0.0 42-43 0.0 0.0 0.0 0.5719581506213223 0.0 44-45 0.0 0.0 0.0 0.722246804001704 0.0 46-47 0.0 0.0 0.0 0.902401032697808 0.0 48-49 0.0 0.0 0.0 1.1011188417423892 0.0 50-51 0.0 0.0 0.0 1.3062875807417094 0.0 52-53 0.0 0.0 0.0 1.4978338772302244 0.0 54-55 0.0 0.0 0.0 1.7051900991264308 0.0 56-57 0.0 0.0 0.0 1.9475288906442128 0.0 58-59 0.0 0.0 0.0 2.2248373842371185 0.0 60-61 0.0 0.0 0.0 2.5063406979734646 0.0 62-63 0.0 0.0 0.0 2.792055988581854 0.0 64-65 0.0 0.0 0.0 3.0808208876994323 0.0 66-67 0.0 0.0 0.0 3.4016388450296184 0.0 68-69 0.0 0.0 0.0 3.75024212433241 0.0 70-71 0.0 0.0 0.0 4.127677286052592 0.0 72-73 0.0 0.0 0.0 4.507377135948373 0.0 74-75 0.0 0.0 0.0 4.87493002199321 0.0 76-77 0.0 0.0 0.0 5.286828762294726 0.0 78-79 0.0 0.0 0.0 5.72788965203924 0.0 80-81 0.0 0.0 0.0 6.195024480078207 0.0 82-83 0.0 0.0 0.0 6.666332682349861 0.0 84-85 0.0 0.0 0.0 7.11897436390142 0.0 86-87 0.0 0.0 0.0 7.59700371460331 0.0 88 0.0 0.0 0.0 7.979371435796862 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 21280 0.0 44.82934 1 GTATCAA 36110 0.0 37.017006 1 GGGCACC 12260 0.0 35.774086 94 GTACTAG 11645 0.0 33.914993 1 CAACGCA 39880 0.0 31.77647 5 ATCAACG 40170 0.0 31.566887 3 CTGTGCG 15980 0.0 30.550127 9 TATCAAC 45470 0.0 29.351547 2 TAGGCAT 14630 0.0 28.776867 5 AACGCAG 46255 0.0 28.32135 6 TAGTACT 17605 0.0 28.164888 4 GTGGTAT 6640 0.0 27.898449 1 ACTGTGC 18375 0.0 27.87234 8 GTACTGT 19000 0.0 26.83218 6 TACCTGG 5790 0.0 26.702684 2 GCATCGT 13945 0.0 26.247818 8 CTAACGC 5805 0.0 26.232182 3 CTAGGCA 16710 0.0 26.101362 4 CTAGTAC 19985 0.0 26.010136 3 TCAACGC 49665 0.0 25.986137 4 >>END_MODULE