FastQCFastQC Report
Mon 6 Jun 2016
ERR1211241_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211241_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12203217
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340290.27885269925135314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269020.22045006656851224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159320.13055573788452668No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA222350.044.17621
GTATCAA383350.039.3491741
GTACTAG118950.035.2546421
CAACGCA428900.033.310055
ATCAACG440700.032.5353623
GTGGTAT69300.032.12411
GGGCACC133950.031.85726494
TATCAAC482650.031.1553542
TAGGCAT148050.030.197625
AACGCAG495350.029.7147146
TAGTACT164000.029.6683624
CTGTGCG162200.029.61739
GCATCGT127900.029.4385418
GTACATG558350.028.7189581
CTAGGCA163050.028.4284524
TACATGG564100.027.9750982
TACCTGG63050.027.6938272
TCAACGC531950.027.2988384
ACATGGG561850.027.1430913
ACGCAGA529400.026.9600227
Produced by FastQC (version 0.11.5)