FastQCFastQC Report
Mon 6 Jun 2016
ERR1211241_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211241_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12203217
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311880.2555719528711159No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264660.21687723819055252No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161650.13246507048100514No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA224650.045.5863841
GTATCAA390900.039.7307321
GTACTAG115700.035.5597231
CAACGCA439450.033.4451375
GGGCACC130900.032.8240694
ATCAACG450350.032.6974643
GTGGTAT65350.031.1548731
TATCAAC498400.031.126382
AACGCAG509450.029.5967396
CTGTGCG165500.029.2142589
TAGTACT170700.028.4702224
CTAACGC58200.028.4265083
TAACGCC59400.028.0896134
TCTAACG59800.027.9775662
TCAACGC540200.027.6940144
TACCTGG62500.027.6712592
ACGCAGA540500.027.1749637
CGCCTAC62100.026.7090727
CGCAGAG548200.026.6904138
ACTGTGC195800.026.4930578
Produced by FastQC (version 0.11.5)