FastQCFastQC Report
Mon 6 Jun 2016
ERR1211240_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211240_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5614378
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182740.32548574392390395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142200.2532782794460936No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91200.16244007795698828No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84200.048.301221
TACCTGG44750.041.968622
GTATCAA150800.041.0469471
GTACCTG56700.034.0374681
CAACGCA178500.033.7100075
ACCTGGG55600.033.6508033
ATCAACG179350.033.4978263
GTACTAG29300.032.933871
TATCAAC191200.032.1514172
AACGCAG198850.030.7332696
TCAACGC198350.030.4783924
GTACATG198050.029.0675261
TACATGG200900.028.326392
ACGCAGA219200.027.4506537
ACATGGG199850.027.2390233
GCATCGT31000.026.9904798
CGCAGAG223600.026.8875378
CATGGGG154850.026.0167394
GTGGTAT37850.025.6187421
CTGTGCG41100.025.160639
Produced by FastQC (version 0.11.5)