##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211240_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5614378 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.423370318136755 31.0 31.0 34.0 28.0 34.0 2 31.43730382956046 31.0 31.0 34.0 28.0 34.0 3 31.50364136508087 33.0 31.0 34.0 28.0 34.0 4 35.09624717110248 37.0 35.0 37.0 32.0 37.0 5 35.154070495431554 37.0 35.0 37.0 32.0 37.0 6 35.21552806027667 37.0 35.0 37.0 32.0 37.0 7 35.1303505036533 37.0 35.0 37.0 32.0 37.0 8 35.130376864543145 37.0 35.0 37.0 32.0 37.0 9 36.70031016792956 39.0 37.0 39.0 32.0 39.0 10-11 36.6467707553713 39.0 37.0 39.0 32.0 39.0 12-13 36.542801179400456 39.0 37.0 39.0 32.0 39.0 14-15 37.73797631723407 40.0 37.0 41.0 32.0 41.0 16-17 37.71085229744061 40.0 37.0 41.0 32.0 41.0 18-19 37.717960297650066 40.0 37.0 41.0 32.0 41.0 20-21 37.685600791396666 40.0 37.0 41.0 32.0 41.0 22-23 37.577801850890694 40.0 37.0 41.0 32.0 41.0 24-25 37.48638059282791 40.0 37.0 41.0 31.5 41.0 26-27 37.34150639661241 40.0 37.0 41.0 31.0 41.0 28-29 37.21717454720719 40.0 36.0 41.0 31.0 41.0 30-31 37.06267871525572 39.5 36.0 41.0 30.5 41.0 32-33 36.871618369835446 39.0 36.0 41.0 30.0 41.0 34-35 36.72323238656179 39.0 35.0 41.0 30.0 41.0 36-37 36.614849142683305 39.0 35.0 41.0 30.0 41.0 38-39 36.47806355396804 39.0 35.0 41.0 29.5 41.0 40-41 36.31463547698427 39.0 35.0 40.5 29.0 41.0 42-43 36.10943999495581 39.0 35.0 40.0 28.0 41.0 44-45 35.90488020222365 38.5 35.0 40.0 27.5 41.0 46-47 35.67908813763519 38.0 34.5 40.0 27.0 41.0 48-49 35.48043799330932 38.0 34.0 40.0 26.0 41.0 50-51 34.807123692063485 37.5 33.5 39.5 25.0 40.5 52-53 34.91785403476574 38.0 33.5 40.0 26.0 40.5 54-55 35.407526888998206 38.0 34.0 40.0 26.0 41.0 56-57 35.37875290904887 38.0 34.0 40.0 26.0 41.0 58-59 35.176320600429825 38.0 34.0 40.0 26.0 41.0 60-61 34.95436796382431 37.5 34.0 40.0 26.0 41.0 62-63 34.65526626457998 37.0 33.5 40.0 26.0 41.0 64-65 34.409015566817914 36.5 33.0 40.0 26.0 41.0 66-67 34.235585936679 36.0 33.0 39.0 25.5 41.0 68-69 33.940769039776086 35.5 33.0 39.0 25.0 41.0 70-71 33.630057327810846 35.0 33.0 39.0 25.0 41.0 72-73 33.28588411752824 35.0 32.5 38.0 24.0 40.0 74-75 32.95610395310041 35.0 32.0 37.0 24.0 39.5 76-77 32.62584742245713 35.0 32.0 37.0 23.0 39.0 78-79 32.291764644275815 35.0 31.5 36.0 23.0 39.0 80-81 31.99718526255268 35.0 31.0 36.0 22.0 38.0 82-83 31.70913661317425 35.0 31.0 35.5 21.0 37.0 84-85 31.46122259669727 35.0 31.0 35.0 20.0 37.0 86-87 31.23879243613451 34.0 31.0 35.0 20.0 36.0 88-89 31.029470495217815 34.0 30.5 35.0 20.0 36.0 90-91 30.8124446733013 34.0 30.0 35.0 19.5 36.0 92-93 30.620382257838713 34.0 30.0 35.0 19.0 35.5 94-95 30.447486168547968 34.0 30.0 35.0 18.0 35.0 96-97 30.276017396762384 34.0 29.0 35.0 18.0 35.0 98-99 30.051103078560082 34.0 29.0 35.0 17.5 35.0 100 28.92021965745805 33.0 27.0 35.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.5599567599497703 1101 2 -0.5096511643616246 1101 3 -0.5481679133855692 1101 4 -0.4747975512256133 1101 5 -0.4561821833098918 1101 6 -0.31643249835692444 1101 7 -0.4168709318681607 1101 8 -0.3869649423871877 1101 9 -0.5955048538827299 1101 10-11 -0.6081554775818034 1101 12-13 -0.628281391416472 1101 14-15 -0.8470054046917284 1101 16-17 -0.7387222689482869 1101 18-19 -0.743319957272 1101 20-21 -0.8573369134838345 1101 22-23 -0.9429249736279814 1101 24-25 -0.9982381643750813 1101 26-27 -0.9468189074641487 1101 28-29 -1.0062044170301192 1101 30-31 -1.0497906639305938 1101 32-33 -1.1155035817595618 1101 34-35 -1.0772087506357266 1101 36-37 -1.1149082211014303 1101 38-39 -1.2004926913191625 1101 40-41 -1.165220098522802 1101 42-43 -1.1190817557223056 1101 44-45 -1.0812422708143075 1101 46-47 -1.2974188230305899 1101 48-49 -1.3095426885746448 1101 50-51 -1.3649300610517727 1101 52-53 -1.1653133633905597 1101 54-55 -1.0239453040424635 1101 56-57 -0.9507556487641509 1101 58-59 -0.9308736751303854 1101 60-61 -0.9473868137610353 1101 62-63 -0.9451277370023448 1101 64-65 -1.0637408663052952 1101 66-67 -1.0314161087510385 1101 68-69 -1.0422702305454905 1101 70-71 -1.1446891996253896 1101 72-73 -1.183237423501403 1101 74-75 -1.126099540134966 1101 76-77 -1.121732540527418 1101 78-79 -1.1863375251341068 1101 80-81 -1.2677281299455245 1101 82-83 -1.2209051459280786 1101 84-85 -1.314903485429582 1101 86-87 -1.3603313489473763 1101 88-89 -1.495853971130959 1101 90-91 -1.4817725564688509 1101 92-93 -1.6078089251554282 1101 94-95 -1.6409965497069159 1101 96-97 -1.669919633786126 1101 98-99 -1.7728954651050124 1101 100 -1.8512681619443647 1102 1 -0.407116600694291 1102 2 -0.4559391331024152 1102 3 -0.43251693948262826 1102 4 -0.30482111984513693 1102 5 -0.3076857072493411 1102 6 -0.3180740911282314 1102 7 -0.31175967078085165 1102 8 -0.36113570232140546 1102 9 -0.5491207414751713 1102 10-11 -0.48590476765102153 1102 12-13 -0.5513707625450124 1102 14-15 -0.6779239620510111 1102 16-17 -0.7385254251853937 1102 18-19 -0.6687295547061964 1102 20-21 -0.7483488904267972 1102 22-23 -0.7422922126603737 1102 24-25 -0.7513811607672309 1102 26-27 -0.7083799446698791 1102 28-29 -0.864299537831144 1102 30-31 -0.8654382844218347 1102 32-33 -0.7447554972644141 1102 34-35 -0.8877058954237143 1102 36-37 -0.873099869031094 1102 38-39 -0.9188901319592659 1102 40-41 -0.9420084632067827 1102 42-43 -1.0086412768250312 1102 44-45 -1.1527051470430436 1102 46-47 -1.0463160505861921 1102 48-49 -1.0633151075604275 1102 50-51 -1.1376025264138931 1102 52-53 -0.9987161521959962 1102 54-55 -0.8046479498091088 1102 56-57 -0.7948892986114302 1102 58-59 -0.7577788904224221 1102 60-61 -0.7395848006914463 1102 62-63 -0.6897330863105537 1102 64-65 -0.7427756822375784 1102 66-67 -0.7456877063765219 1102 68-69 -0.7989916029359918 1102 70-71 -0.8537740430466272 1102 72-73 -0.8755140426729326 1102 74-75 -0.9932714103040965 1102 76-77 -1.0144745696332294 1102 78-79 -1.0633991214422878 1102 80-81 -1.1149403771464037 1102 82-83 -0.9723974705250953 1102 84-85 -0.9853891316244976 1102 86-87 -1.1200857356008598 1102 88-89 -1.2120800964938212 1102 90-91 -1.1792601217776735 1102 92-93 -1.1788183013246112 1102 94-95 -1.1358248332232854 1102 96-97 -1.1793402770276344 1102 98-99 -1.3959909941801456 1102 100 -1.5337430444396176 1103 1 -0.4227711798294074 1103 2 -0.39940995629432763 1103 3 -0.48569747319773526 1103 4 -0.3947020730323061 1103 5 -0.3395040245121663 1103 6 -0.4367810507706906 1103 7 -0.37595049964338045 1103 8 -0.40118803813202675 1103 9 -0.5482654085454612 1103 10-11 -0.5481449114571149 1103 12-13 -0.5393299593943865 1103 14-15 -0.6786323375414867 1103 16-17 -0.6977101409796305 1103 18-19 -0.6801699430350254 1103 20-21 -0.7683671573945077 1103 22-23 -0.80797349696455 1103 24-25 -0.7567602883863387 1103 26-27 -0.7417391140691407 1103 28-29 -0.8372088594476139 1103 30-31 -0.738173537096074 1103 32-33 -0.8428414612713553 1103 34-35 -0.9471160926898108 1103 36-37 -0.8606051570157547 1103 38-39 -0.900811545170626 1103 40-41 -0.9577214528448152 1103 42-43 -1.0135918350149922 1103 44-45 -1.0776689268877035 1103 46-47 -1.0742037078317779 1103 48-49 -1.0423973188477547 1103 50-51 -1.129715471896965 1103 52-53 -1.0625208509513087 1103 54-55 -0.8728792052861536 1103 56-57 -0.8898838514793184 1103 58-59 -0.9946540221987732 1103 60-61 -0.8939331719921739 1103 62-63 -0.9281292614440915 1103 64-65 -0.9443665811701365 1103 66-67 -0.9628521998338613 1103 68-69 -0.985111055179793 1103 70-71 -0.8835900934175882 1103 72-73 -0.9602223648885158 1103 74-75 -0.9601156900478536 1103 76-77 -0.9512354651390424 1103 78-79 -0.8844537205329139 1103 80-81 -0.9181549977637005 1103 82-83 -0.8966782707501153 1103 84-85 -0.9148916615047149 1103 86-87 -1.08864688908357 1103 88-89 -1.0993363130643878 1103 90-91 -1.1470880682517581 1103 92-93 -1.173818926627387 1103 94-95 -1.2563159374447785 1103 96-97 -1.330166241043603 1103 98-99 -1.379685942140057 1103 100 -1.4861047927049107 1104 1 -0.5200641198642835 1104 2 -0.5421596796892132 1104 3 -0.5564954028666342 1104 4 -0.45783159583925226 1104 5 -0.45526595075796905 1104 6 -0.4261174312361007 1104 7 -0.5639940868805198 1104 8 -0.5317255114114303 1104 9 -0.6209736143181814 1104 10-11 -0.6310205931231181 1104 12-13 -0.5878450643610975 1104 14-15 -0.7879584952547418 1104 16-17 -0.8541842672118278 1104 18-19 -0.8731870513747211 1104 20-21 -0.7990090065847397 1104 22-23 -0.7783172213405294 1104 24-25 -0.8293237016755555 1104 26-27 -0.8259492587631527 1104 28-29 -0.8974002361712436 1104 30-31 -0.8560252194638878 1104 32-33 -0.9367025732776213 1104 34-35 -1.1092821076784105 1104 36-37 -0.9493226671042336 1104 38-39 -1.0014949119830234 1104 40-41 -0.9659770861004446 1104 42-43 -1.0217064802264133 1104 44-45 -1.0805775326188822 1104 46-47 -1.1202989656205844 1104 48-49 -1.080606645363531 1104 50-51 -1.0628728163446368 1104 52-53 -0.8582784088056385 1104 54-55 -0.7972048796118258 1104 56-57 -0.7934307973649695 1104 58-59 -0.8351983345172798 1104 60-61 -0.8744242311122647 1104 62-63 -0.7726266099736918 1104 64-65 -0.8136532085212806 1104 66-67 -0.8757014473605338 1104 68-69 -0.983241061374315 1104 70-71 -0.9090775987437993 1104 72-73 -0.8528114066711083 1104 74-75 -0.917821514689166 1104 76-77 -0.850911729734662 1104 78-79 -0.8659399762127151 1104 80-81 -0.9021242095554882 1104 82-83 -0.859572455418494 1104 84-85 -0.850262965827632 1104 86-87 -0.8580929448199477 1104 88-89 -0.9625054020399268 1104 90-91 -0.9629323941767289 1104 92-93 -1.0376336513292088 1104 94-95 -1.0962962975864308 1104 96-97 -1.1569024528604608 1104 98-99 -1.1570922276108568 1104 100 -1.063068151926334 1105 1 -0.5427415135980738 1105 2 -0.47026032149647534 1105 3 -0.5245766716675355 1105 4 -0.4151457646283916 1105 5 -0.3362871882778933 1105 6 -0.37155289589128415 1105 7 -0.4034914659637252 1105 8 -0.42755717064217436 1105 9 -0.6157566739139071 1105 10-11 -0.6071066198691568 1105 12-13 -0.5648964660071343 1105 14-15 -0.7323012804257445 1105 16-17 -0.7827979850705091 1105 18-19 -0.7744011688052979 1105 20-21 -0.7861666016533917 1105 22-23 -0.7797589876090214 1105 24-25 -0.8435700963864363 1105 26-27 -0.8089823017634288 1105 28-29 -0.8539419157940102 1105 30-31 -0.7809987832003245 1105 32-33 -0.7721757736610115 1105 34-35 -0.8185330675942737 1105 36-37 -0.9393429048209825 1105 38-39 -0.9103135312647268 1105 40-41 -0.8942371315894562 1105 42-43 -0.953601366381605 1105 44-45 -0.9143095207307184 1105 46-47 -0.947808096030549 1105 48-49 -0.9807320865987492 1105 50-51 -0.7986726414227476 1105 52-53 -0.8109637567103576 1105 54-55 -0.6180197104721472 1105 56-57 -0.7420574654990233 1105 58-59 -0.6792478169138718 1105 60-61 -0.6265111091758726 1105 62-63 -0.7407594120196563 1105 64-65 -0.6376990573873726 1105 66-67 -0.6548006986436192 1105 68-69 -0.8436194493212454 1105 70-71 -0.6834087146918577 1105 72-73 -0.6482728999822669 1105 74-75 -0.6388043672202315 1105 76-77 -0.6997816813691067 1105 78-79 -0.7426363516495869 1105 80-81 -0.7018026217259532 1105 82-83 -0.8012157137636748 1105 84-85 -0.7398631056952638 1105 86-87 -0.7300393816891031 1105 88-89 -0.8110473023530886 1105 90-91 -0.7647763672327024 1105 92-93 -0.8165079177811236 1105 94-95 -0.8263471087837608 1105 96-97 -0.8711259342019346 1105 98-99 -0.76086387885249 1105 100 -0.9205804762329066 1106 1 -0.6546709617291882 1106 2 -0.5707809276653002 1106 3 -0.7186669061671687 1106 4 -0.5294926578228925 1106 5 -0.5572659922741394 1106 6 -0.596378360368 1106 7 -0.6247104484033343 1106 8 -0.5996013365453194 1106 9 -0.726840887120936 1106 10-11 -0.6863486746608771 1106 12-13 -0.823639268703694 1106 14-15 -1.050961359870513 1106 16-17 -0.991408184677681 1106 18-19 -1.0316739945390765 1106 20-21 -1.0100616490889962 1106 22-23 -1.0000893390803895 1106 24-25 -1.0307471873732368 1106 26-27 -1.0355747829048099 1106 28-29 -1.0195101917489424 1106 30-31 -1.1297973787199211 1106 32-33 -1.1709383643682543 1106 34-35 -1.054239449813167 1106 36-37 -1.0475462939568914 1106 38-39 -1.1092397535988354 1106 40-41 -1.2485060436294049 1106 42-43 -1.2061348025579548 1106 44-45 -1.2741513483701183 1106 46-47 -1.1950597286878022 1106 48-49 -1.0920427934932349 1106 50-51 -1.1336931758746758 1106 52-53 -1.0654544788849378 1106 54-55 -0.9081765655835099 1106 56-57 -0.8717162833117484 1106 58-59 -0.9477870753318314 1106 60-61 -0.9134592915182864 1106 62-63 -1.0241928075076387 1106 64-65 -0.98792459972816 1106 66-67 -0.9545375415192083 1106 68-69 -1.0131752582439972 1106 70-71 -0.9546249894524834 1106 72-73 -0.8499499046160608 1106 74-75 -0.8651717451039147 1106 76-77 -0.8950979090014854 1106 78-79 -0.9664890275682225 1106 80-81 -0.970955425564128 1106 82-83 -0.8278240018958449 1106 84-85 -0.9402560118690637 1106 86-87 -1.0596404600771407 1106 88-89 -0.9933270070550826 1106 90-91 -0.9640486602123488 1106 92-93 -0.9860451388535978 1106 94-95 -1.0976279287567685 1106 96-97 -1.038134549011911 1106 98-99 -0.9485129109670254 1106 100 -1.4047483113484276 1107 1 -0.6232228133796305 1107 2 -0.609157130830237 1107 3 -0.6044573243519551 1107 4 -0.4552798131875164 1107 5 -0.43273502267274466 1107 6 -0.5065474832131827 1107 7 -0.489727578209191 1107 8 -0.5449164705928524 1107 9 -0.6358190806061756 1107 10-11 -0.662366946453794 1107 12-13 -0.7344405629556903 1107 14-15 -0.8384892297927493 1107 16-17 -0.883483610203811 1107 18-19 -0.9117068946423998 1107 20-21 -0.8479946525149558 1107 22-23 -0.8726680546168595 1107 24-25 -0.9192539033447389 1107 26-27 -0.8964562881525069 1107 28-29 -0.9500515479945193 1107 30-31 -0.9464364020490876 1107 32-33 -1.0526842450577973 1107 34-35 -1.035532614204925 1107 36-37 -1.1938253194612685 1107 38-39 -1.1271852645020886 1107 40-41 -0.9492279425907384 1107 42-43 -1.1286618567751532 1107 44-45 -1.160960038347831 1107 46-47 -1.0348267089477332 1107 48-49 -1.0411264848304427 1107 50-51 -1.170660928335387 1107 52-53 -1.0420233515805606 1107 54-55 -0.9555785450277483 1107 56-57 -1.0365729911261994 1107 58-59 -0.8859172074760409 1107 60-61 -0.9669573066360257 1107 62-63 -0.937696804462341 1107 64-65 -0.9634344153788206 1107 66-67 -0.899118541029786 1107 68-69 -0.9489153746179184 1107 70-71 -0.9304284589179233 1107 72-73 -0.9514290497571736 1107 74-75 -0.85689777697079 1107 76-77 -0.8828351432175907 1107 78-79 -0.8087194370521509 1107 80-81 -0.8194722018978027 1107 82-83 -0.8428710408865463 1107 84-85 -0.9074334005253242 1107 86-87 -0.9817351906225724 1107 88-89 -0.860092464665609 1107 90-91 -0.9541500795391222 1107 92-93 -1.0725696915753353 1107 94-95 -1.0945200925503435 1107 96-97 -1.0845155376464213 1107 98-99 -1.080173142627256 1107 100 -0.9501368588496533 1108 1 -0.6846132036820229 1108 2 -0.5994674487671787 1108 3 -0.6742179664278964 1108 4 -0.46301182372337024 1108 5 -0.4442182529733216 1108 6 -0.4303516889885799 1108 7 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56-57 1.1744721821265998 2311 58-59 1.1585097218758236 2311 60-61 1.143569831208147 2311 62-63 1.1671570957424038 2311 64-65 1.1246051043374976 2311 66-67 1.1496278034677019 2311 68-69 1.1847935772966665 2311 70-71 1.1524702272560319 2311 72-73 1.1642187623092468 2311 74-75 1.0734844348707995 2311 76-77 1.1078729472851094 2311 78-79 1.153392938683524 2311 80-81 1.0815731002327595 2311 82-83 1.1243782813671643 2311 84-85 1.1743130415763652 2311 86-87 1.243086557535733 2311 88-89 1.2660761773286886 2311 90-91 1.2702058464408204 2311 92-93 1.2623254323698987 2311 94-95 1.3340902569371913 2311 96-97 1.3572992847508552 2311 98-99 1.4384821798795961 2311 100 1.5593103838253448 2312 1 0.6182410788611534 2312 2 0.6202327176730797 2312 3 0.6657291308844044 2312 4 0.3931566118673473 2312 5 0.4661291132150538 2312 6 0.5813350347960764 2312 7 0.4584417477989433 2312 8 0.5832579155096838 2312 9 0.7940513035739869 2312 10-11 0.739808894137056 2312 12-13 0.8334486614115519 2312 14-15 1.027684183570173 2312 16-17 1.0531379276605364 2312 18-19 1.0139850126146186 2312 20-21 1.0488297916689078 2312 22-23 1.1434547578020968 2312 24-25 1.1472392317360942 2312 26-27 1.2177252329141837 2312 28-29 1.2152155539403822 2312 30-31 1.2144830332767285 2312 32-33 1.1942957565942365 2312 34-35 1.2144433561230557 2312 36-37 1.2722759182401333 2312 38-39 1.2420421699896167 2312 40-41 1.294341578542955 2312 42-43 1.3640625246196407 2312 44-45 1.4036653096058629 2312 46-47 1.429659630819124 2312 48-49 1.437492421322716 2312 50-51 1.409834516135568 2312 52-53 1.3150402744853906 2312 54-55 1.2556168560627157 2312 56-57 1.181444584037287 2312 58-59 1.1564815603100982 2312 60-61 1.1738991432225347 2312 62-63 1.1721437794586862 2312 64-65 1.1782992330358866 2312 66-67 1.1321821285085107 2312 68-69 1.093112348460913 2312 70-71 1.067233038095985 2312 72-73 1.1140604421712226 2312 74-75 1.0631178711978464 2312 76-77 1.0322188397871912 2312 78-79 1.0548356951601434 2312 80-81 1.0507706306459994 2312 82-83 1.060904679093838 2312 84-85 1.133915542082459 2312 86-87 1.130592782052915 2312 88-89 1.0594026465091702 2312 90-91 1.1105505921941905 2312 92-93 1.2031707082529906 2312 94-95 1.1831358938128211 2312 96-97 1.1690572298035384 2312 98-99 0.9189902088881432 2312 100 1.340683517619059 2313 1 0.721802574557433 2313 2 0.7279974234494979 2313 3 0.737214609527431 2313 4 0.6352459125231533 2313 5 0.45995015578313314 2313 6 0.5698180095666885 2313 7 0.6580373193911626 2313 8 0.6214673957383638 2313 9 0.7859004256203761 2313 10-11 0.8242821163437455 2313 12-13 0.8548252104617191 2313 14-15 1.0694332002436724 2313 16-17 1.0602407606628574 2313 18-19 1.011446462366429 2313 20-21 1.0944169951092704 2313 22-23 1.1058144285056173 2313 24-25 1.0617284658500807 2313 26-27 1.098149061099413 2313 28-29 1.1955005132150376 2313 30-31 1.185293312772643 2313 32-33 1.2357038252178612 2313 34-35 1.2758984317265885 2313 36-37 1.2295768572316845 2313 38-39 1.2678606445296055 2313 40-41 1.378519279423962 2313 42-43 1.4530071163970462 2313 44-45 1.435036176938901 2313 46-47 1.5125487142010243 2313 48-49 1.4588865737912755 2313 50-51 1.554062069357208 2313 52-53 1.3700696634149025 2313 54-55 1.280973432211347 2313 56-57 1.2385422395635928 2313 58-59 1.263949394114917 2313 60-61 1.196861676980248 2313 62-63 1.1775004411482328 2313 64-65 1.2618530818252225 2313 66-67 1.262973895151113 2313 68-69 1.2621027901648958 2313 70-71 1.2454940882366827 2313 72-73 1.2538068561500495 2313 74-75 1.2410216980054187 2313 76-77 1.039022459698323 2313 78-79 1.1623701720853674 2313 80-81 1.2635970508107803 2313 82-83 1.2846538965633627 2313 84-85 1.3300853241264754 2313 86-87 1.3597357251868942 2313 88-89 1.319561248356532 2313 90-91 1.3626615279464396 2313 92-93 1.4512203642997505 2313 94-95 1.4592084135935899 2313 96-97 1.4146304990802356 2313 98-99 1.5460472032192563 2313 100 1.6340022549722306 2314 1 0.5863409938152273 2314 2 0.5485680137205691 2314 3 0.5916846774423057 2314 4 0.42728330047182794 2314 5 0.4381400110234708 2314 6 0.4271276564971558 2314 7 0.4913439919810685 2314 8 0.5286417112331634 2314 9 0.6156672936540701 2314 10-11 0.6869832635906619 2314 12-13 0.7412107063458038 2314 14-15 0.8406421640141275 2314 16-17 0.8341633772697037 2314 18-19 0.8781596649521148 2314 20-21 0.8350581133178423 2314 22-23 0.9150248583298222 2314 24-25 0.8519569985620379 2314 26-27 0.9374048013761538 2314 28-29 0.9285409671271125 2314 30-31 0.9927729368521767 2314 32-33 0.9696874333004217 2314 34-35 0.996667334193674 2314 36-37 1.0425515763970807 2314 38-39 1.0154707860908587 2314 40-41 1.0664082623212394 2314 42-43 1.086876297540698 2314 44-45 1.1731249652105404 2314 46-47 1.1735367308705449 2314 48-49 1.1070942870911438 2314 50-51 1.0773838694340583 2314 52-53 1.0527094286625314 2314 54-55 0.9176693369002678 2314 56-57 0.8896414838828335 2314 58-59 0.8930422931330355 2314 60-61 0.8308107740888815 2314 62-63 0.8682005089774023 2314 64-65 0.8648358800573348 2314 66-67 0.8353054197948708 2314 68-69 0.8458514192460527 2314 70-71 0.8065413372588068 2314 72-73 0.8468586158077116 2314 74-75 0.8234120207431062 2314 76-77 0.8114706841811667 2314 78-79 0.845936149672319 2314 80-81 0.8427436644899373 2314 82-83 0.8975579592365648 2314 84-85 0.9423925240998976 2314 86-87 0.9666556437353435 2314 88-89 0.9865454694374058 2314 90-91 1.0391183862691697 2314 92-93 1.109272617854863 2314 94-95 1.1290608292601583 2314 96-97 1.1768296004519385 2314 98-99 1.2661357928716725 2314 100 1.2990205941179163 2315 1 0.42383750514930796 2315 2 0.4497248997859984 2315 3 0.5013928546639832 2315 4 0.14979947815610473 2315 5 0.25408286000865843 2315 6 0.30907628467582526 2315 7 0.41875445879735196 2315 8 0.3812891762943593 2315 9 0.4993417057825056 2315 10-11 0.36376535247399033 2315 12-13 0.44034263473069757 2315 14-15 0.6539349031090893 2315 16-17 0.6429404998594208 2315 18-19 0.5455088676703852 2315 20-21 0.636628967935593 2315 22-23 0.5023323039944216 2315 24-25 0.6367038140514651 2315 26-27 0.7665694521491346 2315 28-29 0.7008317270450277 2315 30-31 0.797426535540346 2315 32-33 0.7842554332631053 2315 34-35 0.842975229875286 2315 36-37 0.8831610537040504 2315 38-39 0.9680072827512021 2315 40-41 0.9766488929153709 2315 42-43 1.0602144405608982 2315 44-45 1.0736877989111733 2315 46-47 1.065927856989454 2315 48-49 1.0498873832160172 2315 50-51 0.9773819485224564 2315 52-53 1.0429212948706237 2315 54-55 0.9094422243309737 2315 56-57 0.9017883122927444 2315 58-59 0.8906159485411251 2315 60-61 0.909338960617184 2315 62-63 0.8125689616503209 2315 64-65 0.8491564996109631 2315 66-67 0.7594731325845672 2315 68-69 0.8186161602512811 2315 70-71 0.764661657704707 2315 72-73 0.5459757577940181 2315 74-75 0.7181981803747775 2315 76-77 0.8156069481493589 2315 78-79 0.8225294347286507 2315 80-81 0.548696710369228 2315 82-83 0.5294929342004622 2315 84-85 0.8736843545354667 2315 86-87 0.9819350543002088 2315 88-89 0.9737322739320824 2315 90-91 1.0866651291116085 2315 92-93 1.1037313599741765 2315 94-95 1.1451788618629344 2315 96-97 1.0000411195317191 2315 98-99 0.41517391123908354 2315 100 0.9637327570661398 2316 1 0.2243573314247307 2316 2 0.21858406187662638 2316 3 0.2739916073406512 2316 4 0.2210008697865291 2316 5 0.11922207687096886 2316 6 0.14329866341400788 2316 7 0.10623553739372937 2316 8 0.17501566576262917 2316 9 0.2545569534040055 2316 10-11 0.20550336611478315 2316 12-13 0.0639253897323897 2316 14-15 0.04285687804976135 2316 16-17 0.16581884371567668 2316 18-19 0.23979986483657711 2316 20-21 0.2892062859058626 2316 22-23 0.2625738085429461 2316 24-25 0.2923172723765646 2316 26-27 0.38896826711745547 2316 28-29 0.3310381880754605 2316 30-31 0.24278160903895696 2316 32-33 0.4202789335211179 2316 34-35 0.4657831742348151 2316 36-37 0.47123733042242577 2316 38-39 0.596675518373651 2316 40-41 0.646968947517955 2316 42-43 0.5413339683994565 2316 44-45 0.5696060596677341 2316 46-47 0.44360140474626775 2316 48-49 0.3355873517128529 2316 50-51 0.4497606975968935 2316 52-53 0.5052984737981276 2316 54-55 0.30734006428728833 2316 56-57 0.15342231317747945 2316 58-59 0.10857632244265858 2316 60-61 0.19035511500180746 2316 62-63 0.35071832450316975 2316 64-65 0.3593173614477436 2316 66-67 0.3283149786084536 2316 68-69 0.3169763524811984 2316 70-71 0.001275708397493247 2316 72-73 -0.05661107562402634 2316 74-75 0.1873285708585115 2316 76-77 0.3925007652088155 2316 78-79 0.3447205505906368 2316 80-81 0.45425470367873544 2316 82-83 0.5264214513764429 2316 84-85 0.6290946938942064 2316 86-87 0.6034512009060009 2316 88-89 0.5782453699729118 2316 90-91 0.500759701462723 2316 92-93 0.5177721995700502 2316 94-95 0.6427567369891456 2316 96-97 0.6157534966779643 2316 98-99 0.4602777329017691 2316 100 0.3568758038144644 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 30.0 1 328.0 2 139.0 3 96.0 4 96.0 5 80.0 6 85.0 7 441.0 8 1386.0 9 2538.0 10 3758.0 11 5751.0 12 8698.0 13 11212.0 14 13755.0 15 16100.0 16 19414.0 17 24269.0 18 29599.0 19 35041.0 20 42395.0 21 50978.0 22 56466.0 23 58031.0 24 57675.0 25 59489.0 26 65363.0 27 74970.0 28 87165.0 29 104236.0 30 126767.0 31 156804.0 32 197644.0 33 260415.0 34 368208.0 35 510716.0 36 766309.0 37 1108129.0 38 1016096.0 39 273375.0 40 331.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.74571849101836 19.095015915260703 12.583401187714536 23.5758644060064 2 15.93267684073833 19.60613654648844 41.14939769393495 23.311788918838275 3 19.57558333109171 25.524196787989222 28.82593778953675 26.074282091382315 4 12.654878339237829 15.956265579576733 38.14468250664156 33.244173574543886 5 13.310444921998938 37.38772466424065 34.43914930216889 14.862681111591517 6 30.849835730431106 36.55655485120934 18.271844217276353 14.321765201083203 7 27.891003343659076 31.88740840223994 22.18860504083071 18.032983213270274 8 26.244611675312342 35.42866918403969 19.96564302605107 18.3610761145969 9 26.679441196862058 15.915319618353486 20.905618447210358 36.4996207375741 10-11 25.741085555856486 25.92088007640443 27.62894138525794 20.70909298248115 12-13 25.73637265572046 23.636731019648007 28.01169493736115 22.615201387270385 14-15 23.03461956248227 25.044151265571635 25.988227219911025 25.933001952035074 16-17 22.216970926596655 27.737945225819615 26.93567274693661 23.10941110064712 18-19 21.768531944475 27.257345345822536 29.086519298208735 21.88760341149373 20-21 22.902799367318455 26.614213992409567 28.724978512895515 21.758008127376463 22-23 22.92663029704962 26.409889794590914 28.475828112805974 22.1876517955535 24-25 22.400944302977948 26.26013512490315 29.118645209208786 22.220275362910115 26-27 22.354262298557305 26.530898553636966 28.502806092863743 22.612033054941985 28-29 21.9949045019437 27.003004336152518 28.725689919979676 22.276401241924106 30-31 22.733140494211487 26.638600335353026 28.71863525125869 21.909623919176806 32-33 22.69285270429549 26.743888986279345 28.26256550696009 22.300692802465072 34-35 22.750468321178808 26.820876390822594 28.5732928212131 21.855362466785493 36-37 22.547627473880173 26.66736799967148 28.32577707889934 22.459227447549008 38-39 22.596443083691696 26.842400805382567 28.520684959986518 22.04047115093922 40-41 22.618278683420847 26.22885807199353 28.79055963637572 22.362303608209906 42-43 22.460624730519932 26.4536155762152 28.328911781579503 22.75684791168537 44-45 22.344346657189572 26.9767172924559 28.395484314189996 22.283451736164537 46-47 22.360648942930244 26.709681719024474 27.957188327381033 22.97248101066425 48-49 22.698891145763778 26.71802113757007 27.93942412776943 22.64366358889672 50-51 22.565021513937694 26.838154007760373 27.904149978095 22.692674500206937 52-53 22.357364354828665 27.08819525324866 27.670899556784452 22.88354083513823 54-55 22.27491985405576 26.896047915180937 27.88534530797112 22.943686922792182 56-57 22.74194982441342 26.83816225379437 27.488132827518548 22.93175509427366 58-59 22.22207472233443 27.025384809883434 28.0291878717554 22.723352596026732 60-61 22.493381166258015 27.305198917755437 27.62020926221627 22.58121065377028 62-63 22.583712920390717 26.992819802930963 27.486227303277253 22.93723997340107 64-65 22.252842375592202 26.914574404166725 27.64623642169109 23.186346798549984 66-67 22.404697594367022 26.692174476985958 27.588131117865682 23.31499681078134 68-69 22.14764293479123 26.79606050784554 27.76268789766472 23.293608659698513 70-71 22.201019438247886 26.56692102583145 27.83749035696019 23.394569178960474 72-73 22.456850825465764 26.76052008784369 27.754592892550956 23.028036194139588 74-75 22.577617600582794 26.938300988045416 27.484145816001764 22.999935595370026 76-77 22.463288018256673 26.758950633229357 27.379326562497212 23.398434786016757 78-79 22.31742923777687 26.757909106788517 27.546844574341744 23.377817081092868 80-81 22.139571136206232 27.047369206341433 27.629535438383957 23.18352421906838 82-83 22.748797857664066 26.569812973160943 28.10698033531719 22.574408833857802 84-85 22.836575415940246 26.797607008677122 27.440486507448618 22.925331067934014 86-87 22.119314210631185 27.153063115854835 27.768961374892125 22.958661298621852 88-89 22.56695581783147 26.419827467724687 27.765129931861964 23.248086782581883 90-91 22.689612250582396 26.42622551296695 27.768727269747313 23.115434966703344 92-93 22.76958454414726 26.570385284673698 27.701189887453094 22.958840283725948 94-95 22.20182752373927 26.593979153640994 27.952841510715665 23.25135181190407 96-97 22.350089942204317 26.45054408040977 27.789317356752097 23.410048620633813 98-99 22.10917465157803 26.68325411669379 27.59448005105402 23.613091180674157 100 22.53872061790502 26.432750163377385 27.299487668481813 23.72904155023578 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1667.0 1 1193.0 2 1685.0 3 2412.0 4 1593.5 5 1140.0 6 1586.0 7 2745.5 8 4363.5 9 5079.5 10 4373.5 11 3674.0 12 3820.5 13 4289.0 14 4841.0 15 5457.5 16 5997.5 17 6539.5 18 7220.0 19 7848.0 20 8605.0 21 9503.0 22 10622.0 23 12418.0 24 14557.5 25 17053.0 26 21135.5 27 26217.5 28 32312.5 29 41492.5 30 53005.0 31 64005.5 32 78242.0 33 93676.0 34 106829.0 35 120523.5 36 134099.0 37 151644.0 38 161650.0 39 162285.0 40 161516.5 41 159113.0 42 161537.5 43 170091.5 44 181443.5 45 199488.0 46 217193.0 47 229790.5 48 240720.5 49 244467.0 50 240903.5 51 240812.0 52 238866.5 53 228038.5 54 213299.0 55 190368.5 56 168561.0 57 144336.0 58 120565.0 59 106610.0 60 88618.0 61 68120.0 62 52655.5 63 41972.0 64 31425.5 65 20772.5 66 14430.0 67 10333.5 68 7776.5 69 6026.0 70 4656.5 71 3508.5 72 2587.0 73 1947.0 74 1507.5 75 1085.0 76 748.5 77 507.0 78 353.5 79 258.5 80 182.0 81 121.0 82 87.0 83 81.0 84 68.5 85 53.0 86 39.0 87 34.0 88 30.0 89 25.5 90 18.0 91 11.0 92 12.5 93 11.5 94 7.0 95 6.0 96 7.0 97 4.5 98 1.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.055161943139560604 2 0.1337815159577784 3 0.01576309967016827 4 0.0153000029566944 5 0.01882666254391849 6 0.05111875260269259 7 0.0562840620991319 8 0.04823330384950924 9 0.03863295275095478 10-11 0.038080798977197475 12-13 0.03929197499705221 14-15 0.01790937482299909 16-17 0.016894124335767913 18-19 0.016787255863427792 20-21 0.020082367093914942 22-23 0.0242146146910664 24-25 0.024027594864471185 26-27 0.01882666254391849 28-29 0.01766001505420547 30-31 0.015861062436480052 32-33 0.021979282477952142 34-35 0.022139585186462328 36-37 0.022397850661284295 38-39 0.020394066804906973 40-41 0.023715895153479157 42-43 0.0306000059133888 44-45 0.04010239424563149 46-47 0.04531223227221252 48-49 0.03817876174350925 50-51 0.026806175145314408 52-53 0.03735943678890164 54-55 0.03491927333713547 56-57 0.03645996048003893 58-59 0.04134028738357125 60-61 0.021890225417668704 62-63 0.024410540223689964 64-65 0.023787140801705905 66-67 0.0306000059133888 68-69 0.037065548489966293 70-71 0.038641858456983126 72-73 0.04211508380803715 74-75 0.025425790710921138 76-77 0.019378816317675795 78-79 0.01878213401377677 80-81 0.022985627259154977 82-83 0.018986965252428677 84-85 0.011595229248903442 86-87 0.014044298406697946 88-89 0.017695637878318845 90-91 0.020064555681858255 92-93 0.019129456548882172 94-95 0.02250471913362442 96-97 0.023671366623337436 98-99 0.02671711808503097 100 0.028605127763039823 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5614378.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.72140276420858 #Duplication Level Percentage of deduplicated Percentage of total 1 80.95610025022279 56.443728717654295 2 12.35263585319533 17.224861990404698 3 3.472752031986512 7.263754293670659 4 1.2803660071546188 3.570756562817149 5 0.6245158375848038 2.1771060122438586 6 0.3675305683366751 1.5374848069895883 7 0.20854000677059278 1.0177791263152298 8 0.14118025975163928 0.7874628601999701 9 0.10467939072832103 0.6568554565873107 >10 0.4586261529323941 5.417650296456491 >50 0.021509566601424838 1.0212715979845288 >100 0.010761541994746035 1.4065988614148783 >500 4.458520247722929E-4 0.21581564604716813 >1k 2.6751053650084797E-4 0.3964832744693182 >5k 2.9723392944389195E-5 0.188998926570707 >10k+ 5.944678588877839E-5 0.6733915701741835 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18274 0.32548574392390395 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14220 0.2532782794460936 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9120 0.16244007795698828 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.7811412056687314E-5 0.0 6 0.0 0.0 0.0 1.7811412056687314E-5 1.7811412056687314E-5 7 0.0 0.0 0.0 1.7811412056687314E-5 1.7811412056687314E-5 8 0.0 0.0 0.0 1.7811412056687314E-5 1.7811412056687314E-5 9 0.0 0.0 0.0 1.7811412056687314E-5 3.562282411337463E-5 10-11 0.0 0.0 0.0 1.7811412056687314E-5 3.562282411337463E-5 12-13 0.0 0.0 0.0 1.7811412056687314E-5 4.452853014171828E-5 14-15 0.0 0.0 0.0 3.562282411337463E-5 6.23399421984056E-5 16-17 0.0 0.0 0.0 5.343423617006194E-5 7.124564822674926E-5 18-19 0.0 0.0 0.0 5.343423617006194E-5 8.905706028343657E-5 20-21 0.0 0.0 0.0 1.6920841453852946E-4 8.905706028343657E-5 22-23 0.0 0.0 0.0 4.363795953888392E-4 9.796276631178022E-5 24-25 0.0 0.0 0.0 9.885333691461457E-4 1.246798843968112E-4 26-27 0.0 0.0 0.0 0.001629744203186889 1.246798843968112E-4 28-29 0.0 0.0 0.0 0.005441386383317974 1.5139700248184215E-4 30-31 0.0 0.0 0.0 0.017606580818035407 1.692084145385295E-4 32-33 0.0 0.0 0.0 0.04641653981972714 2.1373694468024775E-4 34-35 0.0 0.0 0.0 0.0895557798210238 2.1373694468024775E-4 36-37 0.0 0.0 0.0 0.15934979796515303 2.1373694468024775E-4 38-39 0.0 0.0 0.0 0.2625313080095426 2.3154835673693506E-4 40-41 0.0 0.0 0.0 0.37854594044077544 2.3154835673693506E-4 42-43 0.0 0.0 0.0 0.4874716308734467 2.3154835673693506E-4 44-45 0.0 0.0 0.0 0.6118130984411809 2.493597687936224E-4 46-47 0.0 0.0 0.0 0.7620256420212532 2.493597687936224E-4 48-49 0.0 0.0 0.0 0.9251336479303673 2.493597687936224E-4 50-51 0.0 0.0 0.0 1.0913586509494015 2.8498259290699697E-4 52-53 0.0 0.0 0.0 1.259453852234388 3.2060541702037164E-4 54-55 0.0 0.0 0.0 1.4427332822977006 3.384168290770589E-4 56-57 0.0 0.0 0.0 1.6464156848719482 3.473225351054026E-4 58-59 0.0 0.0 0.0 1.8807960561258967 3.5622824113374626E-4 60-61 0.0 0.0 0.0 2.1159957523344524 3.651339471620899E-4 62-63 0.0 0.0 0.0 2.3601011545713524 3.7403965319043355E-4 64-65 0.0 0.0 0.0 2.6058541124234953 3.7403965319043355E-4 66-67 0.0 0.0 0.0 2.886410569434406 3.7403965319043355E-4 68-69 0.0 0.0 0.0 3.1887236662725593 3.7403965319043355E-4 70-71 0.0 0.0 0.0 3.5043329822110305 3.7403965319043355E-4 72-73 0.0 0.0 0.0 3.8097007362169055 4.274738893604955E-4 74-75 0.0 0.0 0.0 4.119815231535888 4.274738893604955E-4 76-77 0.0 0.0 0.0 4.468722982314336 4.274738893604955E-4 78-79 0.0 0.0 0.0 4.848809609898016 4.274738893604955E-4 80-81 0.0 0.0 0.0 5.238772665467128 4.274738893604955E-4 82-83 0.0 0.0 0.0 5.6265360116472385 4.274738893604955E-4 84-85 0.0 0.0 0.0 6.018111000007481 4.274738893604955E-4 86-87 8.905706028343657E-6 0.0 0.0 6.4336512432899955 4.274738893604955E-4 88 1.7811412056687314E-5 0.0 0.0 6.764934601838352 4.274738893604955E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8420 0.0 48.30122 1 TACCTGG 4475 0.0 41.96862 2 GTATCAA 15080 0.0 41.046947 1 GTACCTG 5670 0.0 34.037468 1 CAACGCA 17850 0.0 33.710007 5 ACCTGGG 5560 0.0 33.650803 3 ATCAACG 17935 0.0 33.497826 3 GTACTAG 2930 0.0 32.93387 1 TATCAAC 19120 0.0 32.151417 2 AACGCAG 19885 0.0 30.733269 6 TCAACGC 19835 0.0 30.478392 4 GTACATG 19805 0.0 29.067526 1 TACATGG 20090 0.0 28.32639 2 ACGCAGA 21920 0.0 27.450653 7 ACATGGG 19985 0.0 27.239023 3 GCATCGT 3100 0.0 26.990479 8 CGCAGAG 22360 0.0 26.887537 8 CATGGGG 15485 0.0 26.016739 4 GTGGTAT 3785 0.0 25.618742 1 CTGTGCG 4110 0.0 25.16063 9 >>END_MODULE