FastQCFastQC Report
Mon 6 Jun 2016
ERR1211240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211240_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5614378
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167690.29867956877858953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140650.25051751057730703No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90600.16137139323358704No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88650.047.3092731
GTATCAA157200.039.8094021
TACCTGG46850.038.819042
CAACGCA186700.032.490745
ATCAACG188350.032.166823
GTACCTG58650.031.3450451
ACCTGGG55800.031.334993
TATCAAC203300.030.489542
AACGCAG208000.029.841266
TCAACGC207250.029.3694654
GTACTAG29800.029.3465371
GTACATG198250.027.700641
TACATGG198500.027.178462
ACGCAGA229450.026.498667
CGCAGAG233400.025.9895768
ACATGGG196350.025.8771023
TGGGGGG116650.024.9737996
CTGGGGG39600.024.9173455
CCTGGGG54900.024.4858114
CATGGGG151000.024.4039384
Produced by FastQC (version 0.11.5)