##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211240_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5614378 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16335220036841 33.0 31.0 34.0 30.0 34.0 2 32.303864470828294 34.0 31.0 34.0 30.0 34.0 3 32.38369700080757 34.0 31.0 34.0 30.0 34.0 4 35.99619316690112 37.0 35.0 37.0 35.0 37.0 5 35.88333382611573 37.0 35.0 37.0 35.0 37.0 6 35.908533411893536 37.0 35.0 37.0 35.0 37.0 7 35.848014330349685 37.0 35.0 37.0 35.0 37.0 8 35.83600801371051 37.0 35.0 37.0 35.0 37.0 9 37.445791501747834 39.0 37.0 39.0 34.0 39.0 10-11 37.422566132882395 39.0 37.0 39.0 34.0 39.0 12-13 37.32137629493418 39.0 37.0 39.0 34.0 39.0 14-15 38.555926765173275 40.0 38.0 41.0 34.0 41.0 16-17 38.507606897861166 40.0 38.0 41.0 34.0 41.0 18-19 38.528589542777496 40.0 38.0 41.0 34.0 41.0 20-21 38.48737865530251 40.0 38.0 41.0 34.0 41.0 22-23 38.357950871850804 40.0 38.0 41.0 34.0 41.0 24-25 38.29617252347455 40.0 38.0 41.0 34.0 41.0 26-27 38.16086287741937 40.0 38.0 41.0 33.5 41.0 28-29 38.13809909129738 40.0 37.5 41.0 33.5 41.0 30-31 38.15310226707215 40.0 37.5 41.0 34.0 41.0 32-33 38.0218814087687 40.0 37.0 41.0 33.0 41.0 34-35 37.879739572219755 40.0 37.0 41.0 33.0 41.0 36-37 37.734642555239425 40.0 37.0 41.0 33.0 41.0 38-39 37.64929801662802 40.0 37.0 41.0 33.0 41.0 40-41 37.55021028153074 40.0 36.5 41.0 32.5 41.0 42-43 37.40938648947399 40.0 36.0 41.0 32.0 41.0 44-45 37.276066734373785 40.0 36.0 41.0 31.5 41.0 46-47 37.05080883403291 39.5 36.0 41.0 31.0 41.0 48-49 36.827626497539 39.0 35.5 41.0 31.0 41.0 50-51 36.63673990244334 39.0 35.0 41.0 30.0 41.0 52-53 36.44119838386372 39.0 35.0 40.5 30.0 41.0 54-55 36.20441961691927 38.5 35.0 40.0 29.5 41.0 56-57 35.955238585645645 38.0 35.0 40.0 29.0 41.0 58-59 35.68540130358163 38.0 34.5 40.0 28.0 41.0 60-61 35.42503105419692 37.5 34.0 40.0 28.0 41.0 62-63 35.117667175241856 37.0 34.0 40.0 28.0 41.0 64-65 34.74262625352265 36.5 34.0 39.0 27.0 41.0 66-67 34.43438747800736 36.0 33.5 39.0 26.5 40.5 68-69 34.1018591908133 35.5 33.0 39.0 26.0 40.0 70-71 33.772322419331225 35.0 33.0 38.0 26.0 40.0 72-73 33.426664271625455 35.0 32.5 37.5 26.0 40.0 74-75 33.086234842043055 35.0 32.0 37.0 25.5 39.0 76-77 32.08528994663345 34.0 30.5 36.0 24.5 38.5 78-79 32.580615430596225 35.0 32.0 36.0 25.5 38.5 80-81 32.507364929828384 35.0 32.0 36.0 25.0 37.5 82-83 32.254531134170165 35.0 32.0 35.0 25.0 37.0 84-85 31.99989224095706 34.0 32.0 35.0 25.0 36.5 86-87 31.76143359068449 34.0 31.0 35.0 24.5 36.0 88-89 31.545495689816395 34.0 31.0 35.0 24.0 36.0 90-91 31.33726175900518 34.0 31.0 35.0 24.0 35.5 92-93 31.156930651979614 34.0 31.0 35.0 23.0 35.0 94-95 30.9701130739683 34.0 31.0 35.0 22.5 35.0 96-97 30.79939986228216 34.0 30.0 35.0 20.5 35.0 98-99 30.62822079311368 34.0 30.0 35.0 20.0 35.0 100 28.842581849672396 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.406417823116918 1101 2 -0.40940162385651035 1101 3 -0.4432068678428891 1101 4 -0.20485914678576478 1101 5 -0.2338811246209147 1101 6 -0.3454222528867277 1101 7 -0.3231286150631263 1101 8 -0.34380985964558164 1101 9 -0.6281414377185897 1101 10-11 -0.5596120608207897 1101 12-13 -0.5436184351964357 1101 14-15 -0.8351907546008377 1101 16-17 -0.7672358544153042 1101 18-19 -0.8753858909661787 1101 20-21 -0.7960317714948104 1101 22-23 -0.8923561163820253 1101 24-25 -0.9396970795222686 1101 26-27 -1.0343147519029046 1101 28-29 -1.0043315428084938 1101 30-31 -0.8992708415118287 1101 32-33 -0.8343152969229664 1101 34-35 -0.8799636106560556 1101 36-37 -0.8651166079281154 1101 38-39 -0.8346529511780076 1101 40-41 -0.9423871993665003 1101 42-43 -0.8826766254763143 1101 44-45 -0.8910948828412373 1101 46-47 -0.7937317321698671 1101 48-49 -0.910213738421966 1101 50-51 -0.986447672836384 1101 52-53 -1.021908059778255 1101 54-55 -0.9778440418330447 1101 56-57 -1.009283183193645 1101 58-59 -0.9921850504344292 1101 60-61 -0.9689498623146235 1101 62-63 -1.0135184182344688 1101 64-65 -0.9321893938038315 1101 66-67 -1.0837055090694463 1101 68-69 -1.0976645342092155 1101 70-71 -1.2468257564537737 1101 72-73 -1.1921661314042353 1101 74-75 -1.2857120327084886 1101 76-77 -1.1655549651525874 1101 78-79 -1.0024252401787805 1101 80-81 -0.9607146359622121 1101 82-83 -0.9355162105506345 1101 84-85 -0.8263166398717985 1101 86-87 -1.009347155598519 1101 88-89 -1.103174313112671 1101 90-91 -1.1341517705144533 1101 92-93 -1.2107888173751178 1101 94-95 -1.1277503188585776 1101 96-97 -1.238843096308731 1101 98-99 -1.292713965769515 1101 100 -1.0907157026140126 1102 1 -0.37482225629710797 1102 2 -0.3232865753688827 1102 3 -0.3374367666209821 1102 4 -0.22430179171630726 1102 5 -0.32875966795902656 1102 6 -0.4004000090903901 1102 7 -0.3299759283638153 1102 8 -0.4063704616596766 1102 9 -0.47966879644522464 1102 10-11 -0.5607531794527674 1102 12-13 -0.4937427144384827 1102 14-15 -0.5968008899643849 1102 16-17 -0.655265700982433 1102 18-19 -0.6835250271754418 1102 20-21 -0.6925702235479392 1102 22-23 -0.7636189420539381 1102 24-25 -0.744785738915489 1102 26-27 -0.7794373424089329 1102 28-29 -0.7948904181915069 1102 30-31 -0.8149168416886781 1102 32-33 -0.6946324289168615 1102 34-35 -0.7464481818043183 1102 36-37 -0.7830396761524483 1102 38-39 -0.8390361459838616 1102 40-41 -0.7662100014833371 1102 42-43 -0.7508776208531174 1102 44-45 -0.7987935416570124 1102 46-47 -0.7758270701155752 1102 48-49 -0.7827227775958292 1102 50-51 -0.8829750345705563 1102 52-53 -0.9507784382355808 1102 54-55 -0.878019855343652 1102 56-57 -0.9655238485285693 1102 58-59 -1.0212799570983009 1102 60-61 -0.9470786818956682 1102 62-63 -1.0622655580600053 1102 64-65 -0.9954305466616589 1102 66-67 -1.0700206381893338 1102 68-69 -1.0974906417674148 1102 70-71 -1.0526336512270262 1102 72-73 -0.9693737665361937 1102 74-75 -1.099259673109671 1102 76-77 -0.9551478549573744 1102 78-79 -0.7292557593530802 1102 80-81 -0.7840338624098351 1102 82-83 -0.8558620811324005 1102 84-85 -0.9967676805121393 1102 86-87 -0.9856097940508128 1102 88-89 -1.020282994779766 1102 90-91 -1.0336035741687404 1102 92-93 -1.0833472742583616 1102 94-95 -1.0415210024914003 1102 96-97 -1.1567540779651146 1102 98-99 -1.0896639664010976 1102 100 -1.0851312570908611 1103 1 -0.3574934684860409 1103 2 -0.391450221394237 1103 3 -0.3597563509684605 1103 4 -0.2141738055569462 1103 5 -0.21241768467921673 1103 6 -0.29430971807854434 1103 7 -0.4189043670888779 1103 8 -0.3226718792684693 1103 9 -0.4727281613814043 1103 10-11 -0.43184723808314374 1103 12-13 -0.5266397918590897 1103 14-15 -0.6440260919687901 1103 16-17 -0.6490947865459731 1103 18-19 -0.6294971179034903 1103 20-21 -0.7080397514375747 1103 22-23 -0.7081924539574942 1103 24-25 -0.8855685389806851 1103 26-27 -0.671415272155599 1103 28-29 -0.8036247918299964 1103 30-31 -0.7299390410658972 1103 32-33 -0.7084452430637782 1103 34-35 -0.7746422261227153 1103 36-37 -0.8230270549689962 1103 38-39 -0.8508812880585239 1103 40-41 -0.8125028928517608 1103 42-43 -0.7628580382664225 1103 44-45 -0.804312854483122 1103 46-47 -0.8983843259528967 1103 48-49 -0.9827916737716365 1103 50-51 -1.0184445105397444 1103 52-53 -0.9742455773331571 1103 54-55 -0.980449980580282 1103 56-57 -1.0269637966623009 1103 58-59 -1.0072506008260618 1103 60-61 -1.1498000356919746 1103 62-63 -1.0198187302412975 1103 64-65 -1.0370040386999975 1103 66-67 -1.1639241280108905 1103 68-69 -1.1189012232448192 1103 70-71 -1.2638660078690265 1103 72-73 -1.2532332345909012 1103 74-75 -1.1045051055776476 1103 76-77 -0.995521194575339 1103 78-79 -0.9408390304947467 1103 80-81 -0.8240739517671827 1103 82-83 -0.9650425417073976 1103 84-85 -0.9698912868431826 1103 86-87 -1.0511678801638062 1103 88-89 -1.0301428005431177 1103 90-91 -0.9819571203578441 1103 92-93 -1.1018219237614844 1103 94-95 -1.117257415023282 1103 96-97 -1.130765862552149 1103 98-99 -1.163253073401009 1103 100 -1.2343430595565863 1104 1 -0.36956607330058233 1104 2 -0.3778719537837141 1104 3 -0.38453667816814274 1104 4 -0.29389023607982523 1104 5 -0.33332679349155114 1104 6 -0.36976607176070075 1104 7 -0.35889090384961264 1104 8 -0.41974237150025573 1104 9 -0.5194520468149619 1104 10-11 -0.5708959134221416 1104 12-13 -0.5894488466036307 1104 14-15 -0.8053558016855931 1104 16-17 -0.7084153942536773 1104 18-19 -0.6893635507537965 1104 20-21 -0.7710242579704669 1104 22-23 -0.683263082253923 1104 24-25 -0.708063472362717 1104 26-27 -0.7693169224766052 1104 28-29 -0.8867887157197316 1104 30-31 -0.8338103042113616 1104 32-33 -0.8380031851217211 1104 34-35 -0.7520302055268147 1104 36-37 -0.7944519114744963 1104 38-39 -0.766851742335426 1104 40-41 -0.9643767806127457 1104 42-43 -0.7638335412580588 1104 44-45 -0.9321959480920228 1104 46-47 -0.9540578191263975 1104 48-49 -0.8851874410867566 1104 50-51 -0.9277733089814433 1104 52-53 -0.9754661309730679 1104 54-55 -0.9196997988623608 1104 56-57 -0.9615149086327719 1104 58-59 -0.9343309129870221 1104 60-61 -0.8755870175273444 1104 62-63 -1.0206082632888993 1104 64-65 -1.0145240545264613 1104 66-67 -1.032819121905888 1104 68-69 -0.9413529650514008 1104 70-71 -0.995265656607387 1104 72-73 -1.1067728401466326 1104 74-75 -1.0266770196850459 1104 76-77 -0.940645962280751 1104 78-79 -0.7464039852532522 1104 80-81 -0.814202998347838 1104 82-83 -0.7549244787667568 1104 84-85 -0.7863373855473483 1104 86-87 -0.7771844710997549 1104 88-89 -0.8340743081036557 1104 90-91 -0.8488162916525006 1104 92-93 -0.9850710670912726 1104 94-95 -0.9193679389079996 1104 96-97 -1.014542480199733 1104 98-99 -1.0095928894988901 1104 100 -0.9963395755853597 1105 1 -0.3970811868076467 1105 2 -0.3508020059924206 1105 3 -0.4166149586231249 1105 4 -0.23319353572148316 1105 5 -0.29402279509402973 1105 6 -0.3816139141772794 1105 7 -0.3933218079581806 1105 8 -0.4068060712495196 1105 9 -0.4473404415281763 1105 10-11 -0.5099025329693703 1105 12-13 -0.5038321829066774 1105 14-15 -0.7737969297570615 1105 16-17 -0.6257265176318398 1105 18-19 -0.6609995216576081 1105 20-21 -0.7785887269334779 1105 22-23 -0.7377523557691035 1105 24-25 -0.7303084381178593 1105 26-27 -0.7502742238348716 1105 28-29 -0.7953453743390568 1105 30-31 -0.6955792891170702 1105 32-33 -0.7029373885225709 1105 34-35 -0.7594232609416522 1105 36-37 -0.7452317666922781 1105 38-39 -0.7243709522987487 1105 40-41 -0.778906666401106 1105 42-43 -0.7192211091101584 1105 44-45 -0.6892128399327717 1105 46-47 -0.602621395428784 1105 48-49 -0.7853904733378343 1105 50-51 -0.7923022339804859 1105 52-53 -0.7432614048213182 1105 54-55 -0.7026589484298853 1105 56-57 -0.8094890698054726 1105 58-59 -0.9789589215677381 1105 60-61 -0.8272672487922463 1105 62-63 -0.8648349094121386 1105 64-65 -0.7277429287375909 1105 66-67 -0.8508721202090754 1105 68-69 -0.8551283837517403 1105 70-71 -0.8490017149721325 1105 72-73 -0.7952599783803436 1105 74-75 -0.8643608361498352 1105 76-77 -0.8351625047435611 1105 78-79 -0.6658862013341853 1105 80-81 -0.6195875528152754 1105 82-83 -0.6447565182371449 1105 84-85 -0.7002190733688494 1105 86-87 -0.7430306673639713 1105 88-89 -0.6660918695854399 1105 90-91 -0.6427643234319156 1105 92-93 -0.6821323765131808 1105 94-95 -0.814168350941042 1105 96-97 -0.9660662920527301 1105 98-99 -0.8990264925519895 1105 100 -0.994392230333581 1106 1 -0.4822938932864673 1106 2 -0.4151731951172124 1106 3 -0.4586938999060095 1106 4 -0.3087852001683373 1106 5 -0.3974827697443004 1106 6 -0.4531232768921001 1106 7 -0.4373419355264474 1106 8 -0.4796647362613271 1106 9 -0.6012056898589293 1106 10-11 -0.5019157581516609 1106 12-13 -0.6449393601586664 1106 14-15 -0.81536638830908 1106 16-17 -0.7095778470290313 1106 18-19 -0.720530258936634 1106 20-21 -0.7270897704875949 1106 22-23 -0.7559703517353853 1106 24-25 -0.7480129264250692 1106 26-27 -0.843281094021421 1106 28-29 -0.8103486235538284 1106 30-31 -0.765881027007886 1106 32-33 -0.7518545364730755 1106 34-35 -0.7760758525563389 1106 36-37 -0.7993326562745935 1106 38-39 -0.7916405288177586 1106 40-41 -0.8190302243790981 1106 42-43 -0.7089860093944012 1106 44-45 -0.8536966538669262 1106 46-47 -0.8690674716360434 1106 48-49 -0.9243221403021025 1106 50-51 -0.9589306760259078 1106 52-53 -0.970762348849938 1106 54-55 -0.9295993297296334 1106 56-57 -0.8529822976808035 1106 58-59 -0.9422039357793963 1106 60-61 -1.0216300075219422 1106 62-63 -0.9971018075243734 1106 64-65 -0.9560116827076328 1106 66-67 -0.9231344372212078 1106 68-69 -0.9492503785939732 1106 70-71 -0.978087822090842 1106 72-73 -1.0631865695442357 1106 74-75 -1.0299152809878258 1106 76-77 -1.0030717521258943 1106 78-79 -0.7370431266988433 1106 80-81 -0.8037616814549082 1106 82-83 -0.744104845769705 1106 84-85 -0.7766340185859164 1106 86-87 -0.7410939450898724 1106 88-89 -0.8810244264247409 1106 90-91 -0.8232418116425357 1106 92-93 -0.7159492378887968 1106 94-95 -0.7544148521807195 1106 96-97 -0.8076985394848251 1106 98-99 -0.8674731776211146 1106 100 -1.0658594410729663 1107 1 -0.4235468162295284 1107 2 -0.40579530616185977 1107 3 -0.45822079098219604 1107 4 -0.3169123413940724 1107 5 -0.2848121514398798 1107 6 -0.36145706339490147 1107 7 -0.4288830742506846 1107 8 -0.4276254736586864 1107 9 -0.6272321187022598 1107 10-11 -0.5937668647914975 1107 12-13 -0.5693330194114807 1107 14-15 -0.732830633238116 1107 16-17 -0.7041602060480088 1107 18-19 -0.7482547137737683 1107 20-21 -0.7802941317905407 1107 22-23 -0.7060505627310931 1107 24-25 -0.7307933930083621 1107 26-27 -0.7580045700125027 1107 28-29 -0.7445443383833492 1107 30-31 -0.770676013140907 1107 32-33 -0.7377632318888132 1107 34-35 -0.7816022542094956 1107 36-37 -0.7908901090996565 1107 38-39 -0.780483043364498 1107 40-41 -0.835550970477307 1107 42-43 -0.7731534464491574 1107 44-45 -0.8373213751676971 1107 46-47 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1.0947716101042566 2310 78-79 1.0469719530709511 2310 80-81 0.986341157232836 2310 82-83 0.9715543781442335 2310 84-85 0.9749301129310481 2310 86-87 0.9968656115017573 2310 88-89 0.9908822174158622 2310 90-91 1.0628743584359022 2310 92-93 1.0269637559675893 2310 94-95 1.1096277281354467 2310 96-97 1.2291218388449856 2310 98-99 1.204325103139432 2310 100 1.2848939403407904 2311 1 0.3927692625190389 2311 2 0.3596003104452379 2311 3 0.4194505036771261 2311 4 0.29246373474265397 2311 5 0.38028620682883485 2311 6 0.446398190085489 2311 7 0.49232978020676654 2311 8 0.4963756630668499 2311 9 0.665229720325506 2311 10-11 0.6918529689762849 2311 12-13 0.6952348827062096 2311 14-15 0.8542647386135442 2311 16-17 0.7200172212715756 2311 18-19 0.8557189158255056 2311 20-21 0.7805516622392616 2311 22-23 0.9176133871962264 2311 24-25 0.9239134860433467 2311 26-27 0.3657553879378739 2311 28-29 0.6389841255420734 2311 30-31 0.8534555177324492 2311 32-33 0.9514231344043793 2311 34-35 1.025036951837258 2311 36-37 1.0008244162461963 2311 38-39 1.0239025901226242 2311 40-41 0.9917696732044661 2311 42-43 0.9562667697308811 2311 44-45 1.0360775772452442 2311 46-47 0.5997590446279659 2311 48-49 0.8255514731333946 2311 50-51 0.9817647933657909 2311 52-53 0.8962999872642712 2311 54-55 0.9493024338358964 2311 56-57 1.0776472486227604 2311 58-59 1.0972093643265595 2311 60-61 1.1165829575119588 2311 62-63 1.1633361396855832 2311 64-65 1.1972334283764283 2311 66-67 1.2142652375406442 2311 68-69 1.2224229609315174 2311 70-71 1.1243994642992305 2311 72-73 1.1599703242874497 2311 74-75 1.168001791676879 2311 76-77 1.1513938565173838 2311 78-79 0.8223552306805715 2311 80-81 0.8396785245532428 2311 82-83 0.9232739139719968 2311 84-85 0.9952347546534099 2311 86-87 1.0291761752734239 2311 88-89 1.0722786386930387 2311 90-91 1.1636816686455909 2311 92-93 1.2562464974378535 2311 94-95 1.235059025330898 2311 96-97 1.2390409539855263 2311 98-99 1.2799861691083052 2311 100 1.4243334652093935 2312 1 0.23909102946416283 2312 2 0.3451858958185099 2312 3 0.4540052288780956 2312 4 0.19941928656422192 2312 5 0.3529903350535406 2312 6 0.43928566141971714 2312 7 0.3301731950387534 2312 8 0.4732330672434557 2312 9 0.6354726750440136 2312 10-11 0.6416968876612898 2312 12-13 0.691779577508683 2312 14-15 0.8533770570656216 2312 16-17 0.8552022105051904 2312 18-19 0.8270302486769836 2312 20-21 0.8607801447343704 2312 22-23 0.9235702825472742 2312 24-25 0.9170912738081114 2312 26-27 0.9881822791718449 2312 28-29 0.973560979741201 2312 30-31 0.9711572197799754 2312 32-33 0.8045748551991068 2312 34-35 0.9050639638262723 2312 36-37 0.9807653042482514 2312 38-39 0.8071726079695836 2312 40-41 1.0042872509110694 2312 42-43 1.0659609677149504 2312 44-45 0.8198655762582874 2312 46-47 0.806713370988497 2312 48-49 0.993871302436105 2312 50-51 0.8075947427377486 2312 52-53 1.0244133004607647 2312 54-55 0.8594589134257262 2312 56-57 0.9117133919390099 2312 58-59 1.0399704128592901 2312 60-61 1.05680901029492 2312 62-63 1.127342770372401 2312 64-65 1.1743875708706781 2312 66-67 1.0853505435323711 2312 68-69 1.0844853623496604 2312 70-71 1.0516181791629577 2312 72-73 1.0069619824328413 2312 74-75 1.0614431596118905 2312 76-77 1.1148669756762608 2312 78-79 1.0088087841758266 2312 80-81 0.9505927868221704 2312 82-83 0.9666170564924101 2312 84-85 0.975763875731559 2312 86-87 0.981224297713954 2312 88-89 0.9973403300507933 2312 90-91 1.0165231592681074 2312 92-93 1.0386901185714983 2312 94-95 1.1095364663608862 2312 96-97 0.8212578176961145 2312 98-99 0.5658899383671887 2312 100 0.8115621817204293 2313 1 0.01827302877578063 2313 2 0.21466899687451502 2313 3 0.3165623977801957 2313 4 0.22966506585201785 2313 5 0.3370048553975309 2313 6 0.24913619218979477 2313 7 0.3022654288472708 2313 8 0.373987380474901 2313 9 0.6049719163186751 2313 10-11 0.431274842328655 2313 12-13 0.4502354875862764 2313 14-15 0.5332886752992536 2313 16-17 0.7586966691426795 2313 18-19 0.7175915411010507 2313 20-21 0.6934594502952791 2313 22-23 0.6649078361988785 2313 24-25 0.5512066536356244 2313 26-27 0.7513193141303915 2313 28-29 0.592436571521084 2313 30-31 0.8572099210830615 2313 32-33 0.9162710323534284 2313 34-35 1.0004495045765012 2313 36-37 1.0154544272040695 2313 38-39 1.0144082769974077 2313 40-41 1.0474708561406771 2313 42-43 1.072609972435231 2313 44-45 0.8863706831630296 2313 46-47 0.9663453109730042 2313 48-49 1.030188077525871 2313 50-51 1.0061586121942199 2313 52-53 1.0904156181691533 2313 54-55 0.9500979242666858 2313 56-57 0.648703734145414 2313 58-59 0.7502416473203937 2313 60-61 0.6681597822678427 2313 62-63 0.9746560317568225 2313 64-65 1.101089176496025 2313 66-67 1.0461750926023399 2313 68-69 1.1671614938926638 2313 70-71 1.0380239505629874 2313 72-73 1.139476340361668 2313 74-75 1.1439782387000932 2313 76-77 1.1407241799877958 2313 78-79 1.0293657387754394 2313 80-81 0.9590005801391257 2313 82-83 0.9598801472339744 2313 84-85 0.9951418627694082 2313 86-87 1.0112996367429652 2313 88-89 0.9788671407098128 2313 90-91 0.8444339081359864 2313 92-93 1.019057197117636 2313 94-95 1.1648339359933004 2313 96-97 1.2508520003741275 2313 98-99 1.2466397336880561 2313 100 1.365243490964545 2314 1 -0.31741661066052274 2314 2 0.07663119755078895 2314 3 0.2931131142893335 2314 4 0.23799055116369772 2314 5 0.33058114033271124 2314 6 0.4238175156829982 2314 7 0.4896984290958102 2314 8 0.4778154700574717 2314 9 0.4256985393085486 2314 10-11 0.5127192056643608 2314 12-13 0.46736014474060283 2314 14-15 0.7786817673084627 2314 16-17 0.8292687614274641 2314 18-19 0.8633510272674627 2314 20-21 0.9078363898257038 2314 22-23 0.9611812783257108 2314 24-25 0.8573387836615964 2314 26-27 0.7527236638207384 2314 28-29 0.7965623778926059 2314 30-31 0.8321820322225335 2314 32-33 0.8604727359197142 2314 34-35 0.8761345664051277 2314 36-37 0.870929507957193 2314 38-39 0.6614012477499287 2314 40-41 0.6797146044282769 2314 42-43 0.8523591100805632 2314 44-45 0.8839545726169433 2314 46-47 0.7855352634908144 2314 48-49 0.8877766682827115 2314 50-51 0.8581638093383788 2314 52-53 0.8611643472667581 2314 54-55 0.8413803051422022 2314 56-57 0.7667354397111481 2314 58-59 0.8557101678077288 2314 60-61 0.9630076698048313 2314 62-63 0.8830266128214319 2314 64-65 0.856679671541194 2314 66-67 0.5672758892969227 2314 68-69 0.5280281080948797 2314 70-71 0.7509075350462666 2314 72-73 0.8880926323156686 2314 74-75 1.0169048523421367 2314 76-77 0.7880579421902176 2314 78-79 0.6555689953355568 2314 80-81 0.7391572052698123 2314 82-83 0.755908319356621 2314 84-85 0.8430270915159355 2314 86-87 0.8843541866119828 2314 88-89 0.8331278544190219 2314 90-91 0.9308063784687839 2314 92-93 0.8513801501225444 2314 94-95 0.8139068644137808 2314 96-97 0.8146552126336672 2314 98-99 0.9534612129082731 2314 100 1.0143930993939883 2315 1 0.422492222390737 2315 2 0.4347937692505184 2315 3 0.4408161000768729 2315 4 0.27693704490812365 2315 5 0.37201997837526335 2315 6 0.43780382048541355 2315 7 0.39227399529385565 2315 8 0.24741994621717822 2315 9 0.47877123654920695 2315 10-11 0.5359328062817994 2315 12-13 0.3500833425668617 2315 14-15 0.5907613506101441 2315 16-17 0.7003014188610237 2315 18-19 0.7325763885286634 2315 20-21 0.46405709372341164 2315 22-23 0.8043014619094109 2315 24-25 0.8077069165786455 2315 26-27 0.8135671526081154 2315 28-29 0.7641426048867075 2315 30-31 0.8207315397694472 2315 32-33 0.7785986536335798 2315 34-35 0.8121969687037804 2315 36-37 0.8471308764383778 2315 38-39 0.848929780878457 2315 40-41 0.8219388664791651 2315 42-43 0.8767023299403149 2315 44-45 0.9021288717790554 2315 46-47 0.914604966661777 2315 48-49 0.8080294290045416 2315 50-51 0.8692989136304519 2315 52-53 0.8945677678360298 2315 54-55 0.7052439906538055 2315 56-57 0.6479310177330291 2315 58-59 0.6696771952830574 2315 60-61 0.7698730452785796 2315 62-63 0.8394230636280966 2315 64-65 0.9140332292059057 2315 66-67 0.9275698807470434 2315 68-69 0.9219622345131242 2315 70-71 0.7990768248757831 2315 72-73 0.9655151614886677 2315 74-75 0.3176566570968973 2315 76-77 0.3114596183165155 2315 78-79 0.6313127885907193 2315 80-81 0.7038752409706959 2315 82-83 0.6793614222251563 2315 84-85 0.697400901617911 2315 86-87 0.7172798744853601 2315 88-89 0.7189050376505755 2315 90-91 0.8021432902572734 2315 92-93 0.9003966103766565 2315 94-95 0.868214941052738 2315 96-97 0.9795408627242814 2315 98-99 0.9599848531470982 2315 100 0.9169212919072649 2316 1 0.23594425470691505 2316 2 0.053864138547076834 2316 3 0.12884727483658054 2316 4 -0.04975971936462287 2316 5 0.24485391753488273 2316 6 0.3518813970745285 2316 7 0.3909425080779556 2316 8 0.4422463752546193 2316 9 0.6092479410901319 2316 10-11 0.5927658826668107 2316 12-13 0.3789386601289735 2316 14-15 0.5171550259728761 2316 16-17 0.8220057281626154 2316 18-19 0.7615682496068743 2316 20-21 0.6925719447727658 2316 22-23 0.7424680253449836 2316 24-25 0.4900959559332918 2316 26-27 0.7200117581685106 2316 28-29 0.9071061938198852 2316 30-31 0.6010322706264404 2316 32-33 0.7243388336675096 2316 34-35 0.8315516226249571 2316 36-37 0.8624512049342101 2316 38-39 0.751415670364949 2316 40-41 0.798493506960618 2316 42-43 0.775148435747127 2316 44-45 0.635820936985084 2316 46-47 0.8013741015038462 2316 48-49 0.8798509262640621 2316 50-51 0.8833969308917915 2316 52-53 0.9029913359550577 2316 54-55 0.7883406180540149 2316 56-57 0.993491476299603 2316 58-59 0.8446864133049985 2316 60-61 0.9035143389140572 2316 62-63 0.8809975845753115 2316 64-65 0.9240418373414556 2316 66-67 0.944666800212886 2316 68-69 0.9413414560261728 2316 70-71 0.8870521293363396 2316 72-73 0.9196268110942682 2316 74-75 0.978265583872485 2316 76-77 0.8269186039039731 2316 78-79 0.5315372285226232 2316 80-81 0.5789574797587917 2316 82-83 0.5252081722236426 2316 84-85 0.5981275009485358 2316 86-87 0.6457596271072568 2316 88-89 0.5877741705882507 2316 90-91 0.4338283172132016 2316 92-93 0.2871337583230691 2316 94-95 0.34038318236222764 2316 96-97 0.590742314057767 2316 98-99 0.8007533566183938 2316 100 0.35604125222259597 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 4.0 12 23.0 13 111.0 14 521.0 15 1400.0 16 3421.0 17 7020.0 18 11943.0 19 17232.0 20 24224.0 21 33122.0 22 40600.0 23 43182.0 24 44355.0 25 47217.0 26 55339.0 27 68226.0 28 83255.0 29 103472.0 30 128311.0 31 160475.0 32 203808.0 33 266760.0 34 381215.0 35 534174.0 36 828837.0 37 1265137.0 38 1092335.0 39 168629.0 40 28.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.70641833191047 19.936791922794626 13.416089014478235 23.940700730816662 2 15.58024771399432 20.040100613104425 40.76476503719557 23.614886635705684 3 19.03640973229804 25.424472666428944 29.539193834116617 25.999923767156396 4 12.585266601219736 16.359927236316278 38.01362626476787 33.04117989769612 5 13.148847476960048 37.550375126149326 34.438311777368746 14.862465619521878 6 31.007780381014605 36.854020160381076 17.898794844237422 14.239404614366899 7 27.802519016951795 32.035773439160096 22.051257023764 18.110450520124118 8 26.285102285596018 35.853731259277524 19.69156690197917 18.169599553147293 9 26.536021364783874 15.921017848401526 20.754315673517258 36.78864511329734 10-11 25.71953374813369 26.128824409247915 27.460608735031094 20.6910331075873 12-13 25.628707446740297 23.66998576511441 28.017165220142008 22.684141568003284 14-15 22.90666146097629 25.194033381133302 25.864696460100863 26.034608697789547 16-17 22.101521811925135 27.90952129895482 26.881648130864427 23.107308758255616 18-19 21.733992616813474 27.3302046994342 29.03700107117832 21.898801612574 20-21 22.782579190658268 26.649098675676868 28.73527831001745 21.833043823647415 22-23 22.803692590701942 26.432990439902692 28.494679196876305 22.268637772519057 24-25 22.299819070645093 26.295867764473368 29.116543583170685 22.287769581710855 26-27 22.326640185709866 26.597259985084648 28.495547747387988 22.5805520818175 28-29 21.98290955737478 27.051905771729167 28.71741885014265 22.247765820753404 30-31 22.723681612572182 26.615921259448417 28.74841107039418 21.911986057585224 32-33 22.653551150938878 26.75455473087754 28.293299514884108 22.29859460329947 34-35 22.65546219086258 26.82077779733598 28.61875979444406 21.905000217357376 36-37 22.523342830730304 26.649797450927515 28.411737859728053 22.41512185861413 38-39 22.523614801132563 26.78599265184672 28.619548054545866 22.07084449247485 40-41 22.511232487783417 26.20265768008272 28.96023454414134 22.325875287992524 42-43 22.44298022502266 26.454242044696752 28.41684050635797 22.685937223922622 44-45 22.24206250419174 26.94446168890341 28.500479940260643 22.312995866644208 46-47 22.257793101531668 26.670175556110582 28.069114373881465 23.00291696847629 48-49 22.645235842230065 26.73890923664377 28.049820209959144 22.56603471116702 50-51 22.52768261736572 26.816193726618305 27.926535597802292 22.72958805821368 52-53 22.259894553692426 27.080952463657365 27.731889024419026 22.927263958231183 54-55 22.17819778629508 26.92845480909861 27.889060559052542 23.004286845553764 56-57 22.737274287154982 26.76641282612961 27.499952576891562 22.996360309823853 58-59 22.126865571942474 27.02120363847204 28.018211954445178 22.833718835140306 60-61 22.4544890301915 27.327733933416205 27.581665386707982 22.636111649684317 62-63 22.585481885687155 26.998326684690273 27.427720998695566 22.98847043092701 64-65 22.134968828874946 26.93868165700653 27.565471746070326 23.360877768048198 66-67 22.294361300043256 26.765383080602987 27.539462772689188 23.40079284666457 68-69 22.054097636519938 26.80306816032017 27.734945623178962 23.407888579980927 70-71 22.095383497324804 26.566288903830777 27.780422210694038 23.557905388150377 72-73 22.284729622330172 26.761401908128974 27.66316569787126 23.290702771669594 74-75 22.379532482064285 26.96753887148201 27.38354591302659 23.269382733427115 76-77 22.179189270473135 26.758295228755745 27.3269597250769 23.73555577569422 78-79 22.033496428427643 26.726233251518572 27.49606573018516 23.744204589868627 80-81 21.834285958559466 27.03781052346593 27.53594831778081 23.59195520019379 82-83 22.445056464998935 26.503950727999666 27.985234293863886 23.06575851313751 84-85 22.5076498486871 26.763265507244157 27.321238221825965 23.407846422242777 86-87 21.7409091378941 27.1214031395772 27.608921215439324 23.528766507089376 88-89 22.070385972035652 26.47027343090997 27.60522056073251 23.854120036321873 90-91 22.20132793235988 26.403361191792474 27.617323887866895 23.777986987980746 92-93 22.240545661937528 26.54734098231515 27.502862332136722 23.709251023610598 94-95 21.598782890169126 26.51873080619097 27.83922300495054 24.04326329868936 96-97 21.648926577270807 26.424647999903815 27.69977026869968 24.2266551541257 98-99 21.35413465452722 26.64344422561131 27.566106459793644 24.436314660067822 100 21.564428239727814 26.39898714186143 27.3898591605507 24.646725457860054 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1644.0 1 1157.0 2 1451.0 3 2078.5 4 1388.0 5 979.5 6 1428.0 7 2556.5 8 4019.0 9 4546.5 10 3839.0 11 3215.0 12 3292.0 13 3741.5 14 4331.0 15 4962.5 16 5659.0 17 6297.0 18 7082.5 19 7816.0 20 8441.5 21 9370.5 22 10760.5 23 12471.5 24 14487.5 25 17190.0 26 21311.0 27 26254.5 28 32114.5 29 41008.5 30 52900.0 31 64715.5 32 79263.0 33 94450.0 34 107415.0 35 121369.0 36 134624.0 37 151786.5 38 161855.0 39 162142.0 40 160800.5 41 158267.0 42 161069.5 43 169780.5 44 181365.5 45 200498.0 46 218852.0 47 230436.5 48 241653.5 49 245860.0 50 241699.0 51 241410.0 52 240049.5 53 229598.5 54 216794.5 55 194409.0 56 170369.5 57 145735.0 58 121901.5 59 107389.0 60 87978.5 61 66547.5 62 51185.5 63 39997.5 64 29042.0 65 18794.5 66 12875.0 67 9224.5 68 6857.0 69 5225.5 70 3960.5 71 2912.5 72 2155.0 73 1644.0 74 1232.0 75 829.0 76 549.0 77 330.5 78 198.0 79 125.5 80 74.5 81 37.5 82 22.0 83 11.5 84 8.0 85 7.0 86 6.5 87 8.0 88 5.5 89 1.0 90 0.5 91 0.5 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04340641118214698 2 0.0 3 0.0 4 0.030065663551688184 5 0.0 6 0.0 7 8.015135425509291E-4 8 0.0 9 0.011648663485073502 10-11 5.43248067728963E-4 12-13 3.562282411337463E-5 14-15 1.6030270851018582E-4 16-17 3.2060541702037164E-4 18-19 0.0 20-21 8.905706028343657E-6 22-23 0.0 24-25 5.69965185813994E-4 26-27 5.16530949643932E-4 28-29 2.1373694468024773E-4 30-31 0.002538126218077942 32-33 0.015567174137544711 34-35 0.026752740909144344 36-37 0.03183789905132857 38-39 0.022567059075822824 40-41 0.0014783472007050468 42-43 1.7811412056687313E-4 44-45 0.011728814839328595 46-47 0.018096394649594307 48-49 0.0033930739967989332 50-51 0.010891678472664292 52-53 0.011051981181174477 54-55 0.010268279050680236 56-57 4.7200241950221374E-4 58-59 0.008602912023379972 60-61 0.0047111184889937945 62-63 0.001326950198223205 64-65 0.03655792324635071 66-67 0.061574051479968035 68-69 0.0604430268143684 70-71 0.05359453887857212 72-73 0.04627404852327364 74-75 0.035141915987844066 76-77 0.031241216747429548 78-79 0.015745288258111584 80-81 0.0012111760198547372 82-83 0.03890902963783344 84-85 0.05330065057963678 86-87 0.058848905435294876 88-89 0.0709695713398706 90-91 0.049818519522554415 92-93 0.040227074130028295 94-95 0.013385276160600515 96-97 0.0037493022379326796 98-99 0.02267392754816295 100 0.03109872545097605 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5614378.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.64620473211426 #Duplication Level Percentage of deduplicated Percentage of total 1 79.85523147739904 54.81758568927909 2 13.064797353621508 17.93697507840573 3 3.6275419912875617 7.470509706248022 4 1.395524671242968 3.831898891634449 5 0.6617846590034643 2.271450259526211 6 0.3898895901663273 1.6058664377686698 7 0.22641510646123322 1.087977642680688 8 0.1484651690500283 0.8153256312156956 9 0.11440921421074501 0.7068382507756005 >10 0.48127766452239534 5.58079861572232 >50 0.02263546615977602 1.0456283092527834 >100 0.011176860439388751 1.3990769131637042 >500 4.861585745623702E-4 0.22316056123304262 >1k 2.7346339573368914E-4 0.37479788616846166 >5k 3.03848217478845E-5 0.1889737241175394 >10k+ 6.0769643495769E-5 0.6431364028079701 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16769 0.29867956877858953 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14065 0.25051751057730703 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9060 0.16137139323358704 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.7811412056687314E-5 2 0.0 0.0 0.0 0.0 1.7811412056687314E-5 3 0.0 0.0 0.0 0.0 1.7811412056687314E-5 4 0.0 0.0 0.0 0.0 3.562282411337463E-5 5 0.0 0.0 0.0 0.0 3.562282411337463E-5 6 0.0 0.0 0.0 0.0 3.562282411337463E-5 7 0.0 0.0 0.0 0.0 3.562282411337463E-5 8 0.0 0.0 0.0 0.0 5.343423617006194E-5 9 0.0 0.0 0.0 0.0 5.343423617006194E-5 10-11 0.0 0.0 0.0 0.0 7.124564822674926E-5 12-13 0.0 0.0 0.0 0.0 1.1577417836846753E-4 14-15 0.0 0.0 0.0 1.7811412056687314E-5 1.424912964534985E-4 16-17 0.0 0.0 0.0 3.562282411337463E-5 1.424912964534985E-4 18-19 0.0 0.0 0.0 3.562282411337463E-5 1.424912964534985E-4 20-21 0.0 0.0 0.0 1.6920841453852946E-4 1.6030270851018582E-4 22-23 0.0 0.0 0.0 4.5419100744552646E-4 1.692084145385295E-4 24-25 0.0 0.0 0.0 0.0010241561932595204 1.7811412056687313E-4 26-27 0.0 0.0 0.0 0.001683178439356951 1.8701982659521677E-4 28-29 0.0 0.0 0.0 0.005574971973743128 1.9592553262356044E-4 30-31 0.0 0.0 0.0 0.017918280529027435 1.9592553262356044E-4 32-33 0.0 0.0 0.0 0.04671933382469082 2.1373694468024775E-4 34-35 0.0 0.0 0.0 0.08991200806215754 2.3154835673693506E-4 36-37 0.0 0.0 0.0 0.1602759913921008 2.3154835673693506E-4 38-39 0.0 0.0 0.0 0.2637513897354257 2.3154835673693506E-4 40-41 0.0 0.0 0.0 0.380166778937934 2.3154835673693506E-4 42-43 0.0 0.0 0.0 0.49003647420960966 2.4045406276527873E-4 44-45 0.0 0.0 0.0 0.6151260210837247 2.493597687936224E-4 46-47 0.0 0.0 0.0 0.7661578896184047 2.493597687936224E-4 48-49 0.0 0.0 0.0 0.9295152552963124 2.493597687936224E-4 50-51 0.0 0.0 0.0 1.0965684889759828 2.8498259290699697E-4 52-53 0.0 0.0 0.0 1.2647438416152244 3.2060541702037164E-4 54-55 0.0 0.0 0.0 1.447640326319318 3.384168290770589E-4 56-57 0.0 0.0 0.0 1.6510733691247723 3.384168290770589E-4 58-59 0.0 0.0 0.0 1.885364683318437 3.384168290770589E-4 60-61 0.0 0.0 0.0 2.119843017338697 3.384168290770589E-4 62-63 0.0 0.0 0.0 2.3632537745053863 3.918510652471209E-4 64-65 0.0 0.0 0.0 2.6082408416390916 3.918510652471209E-4 66-67 0.0 0.0 0.0 2.888396541878726 3.918510652471209E-4 68-69 0.0 0.0 0.0 3.1907452615409935 3.918510652471209E-4 70-71 0.0 0.0 0.0 3.506906731253222 4.0075677127546455E-4 72-73 0.0 0.0 0.0 3.812452599379664 4.452853014171828E-4 74-75 0.0 0.0 0.0 4.123030191412121 4.452853014171828E-4 76-77 0.0 0.0 0.0 4.4725257187884395 4.452853014171828E-4 78-79 0.0 0.0 0.0 4.853315897148358 4.5419100744552646E-4 80-81 0.0 0.0 0.0 5.244347637440871 4.720024195022138E-4 82-83 0.0 0.0 0.0 5.632876874339419 5.16530949643932E-4 84-85 0.0 0.0 0.0 6.024612165408172 5.16530949643932E-4 86-87 0.0 0.0 0.0 6.440321617105225 5.16530949643932E-4 88 0.0 0.0 0.0 6.771275464530532 5.343423617006194E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8865 0.0 47.309273 1 GTATCAA 15720 0.0 39.809402 1 TACCTGG 4685 0.0 38.81904 2 CAACGCA 18670 0.0 32.49074 5 ATCAACG 18835 0.0 32.16682 3 GTACCTG 5865 0.0 31.345045 1 ACCTGGG 5580 0.0 31.33499 3 TATCAAC 20330 0.0 30.48954 2 AACGCAG 20800 0.0 29.84126 6 TCAACGC 20725 0.0 29.369465 4 GTACTAG 2980 0.0 29.346537 1 GTACATG 19825 0.0 27.70064 1 TACATGG 19850 0.0 27.17846 2 ACGCAGA 22945 0.0 26.49866 7 CGCAGAG 23340 0.0 25.989576 8 ACATGGG 19635 0.0 25.877102 3 TGGGGGG 11665 0.0 24.973799 6 CTGGGGG 3960 0.0 24.917345 5 CCTGGGG 5490 0.0 24.485811 4 CATGGGG 15100 0.0 24.403938 4 >>END_MODULE