FastQCFastQC Report
Mon 6 Jun 2016
ERR1211239_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211239_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8064775
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325360.4034334497862619No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252950.31364793190138596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158930.19706687415333968No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA167500.048.267581
GTATCAA301650.040.9674151
TACCTGG54250.036.780822
GTACTAG71200.036.219941
CAACGCA348400.033.9335945
ACCTGGG56850.033.8187453
ATCAACG350250.033.767363
TATCAAC377050.032.600892
GCATCGT74600.032.5777978
GGGCACC80750.031.42776594
AACGCAG396700.030.5489396
GTGGTAT54350.030.1319261
TAGGCAT91100.029.9281375
TCAACGC414200.028.996314
TCTAACG38100.028.77972
CTGTGCG98850.028.3903269
GTACATG424750.028.2079941
GTACCTG71300.028.1168861
ACGCAGA423100.027.8876257
TAGTACT103600.027.7685784
Produced by FastQC (version 0.11.5)