##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211239_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8064775 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.49703891305089 31.0 31.0 34.0 30.0 34.0 2 31.512405367787693 31.0 31.0 34.0 28.0 34.0 3 31.575994990560805 33.0 31.0 34.0 28.0 34.0 4 35.17871385128537 37.0 35.0 37.0 32.0 37.0 5 35.24633433666779 37.0 35.0 37.0 32.0 37.0 6 35.306191059267 37.0 35.0 37.0 32.0 37.0 7 35.22646112259797 37.0 35.0 37.0 32.0 37.0 8 35.23562380847575 37.0 35.0 37.0 32.0 37.0 9 36.79906643396747 39.0 37.0 39.0 32.0 39.0 10-11 36.7475015731995 39.0 37.0 39.0 32.0 39.0 12-13 36.631526979983946 39.0 37.0 39.0 32.0 39.0 14-15 37.808859391613524 40.0 37.0 41.0 32.0 41.0 16-17 37.79378240558478 40.0 37.0 41.0 32.0 41.0 18-19 37.81679736880446 40.0 37.0 41.0 32.0 41.0 20-21 37.78921836009064 40.0 37.0 41.0 32.0 41.0 22-23 37.67403808537746 40.0 37.0 41.0 32.0 41.0 24-25 37.572928259002886 40.0 37.0 41.0 32.0 41.0 26-27 37.42094243174794 40.0 37.0 41.0 31.0 41.0 28-29 37.3041370032022 40.0 36.0 41.0 31.0 41.0 30-31 37.14654141002074 39.5 36.0 41.0 31.0 41.0 32-33 36.95447777526341 39.0 35.5 41.0 30.0 41.0 34-35 36.80666924991708 39.0 35.0 41.0 30.0 41.0 36-37 36.69998040862888 39.0 35.0 41.0 30.0 41.0 38-39 36.55340130381815 39.0 35.0 41.0 30.0 41.0 40-41 36.37950742581163 39.0 35.0 40.0 29.5 41.0 42-43 36.1593010964348 39.0 35.0 40.0 28.5 41.0 44-45 35.905330402894066 38.0 35.0 40.0 28.0 41.0 46-47 35.63334606359136 38.0 34.0 40.0 27.0 41.0 48-49 35.39159933166145 38.0 34.0 40.0 26.0 41.0 50-51 34.67518883043854 37.5 33.5 39.5 25.0 40.5 52-53 34.76252834084026 37.5 33.5 39.5 26.0 40.5 54-55 35.228488953504595 38.0 34.0 40.0 26.0 41.0 56-57 35.15821538728607 38.0 34.0 40.0 26.0 41.0 58-59 34.91685837484617 37.0 34.0 40.0 26.0 41.0 60-61 34.6878258847891 37.0 34.0 40.0 26.0 41.0 62-63 34.37450567436785 36.0 33.0 40.0 26.0 41.0 64-65 34.12077212321485 36.0 33.0 39.0 25.0 41.0 66-67 33.9511143832283 35.0 33.0 39.0 25.0 41.0 68-69 33.66744744893689 35.0 33.0 39.0 25.0 41.0 70-71 33.39760569885706 35.0 33.0 38.0 24.5 40.5 72-73 33.09521896147134 35.0 32.0 37.0 24.0 40.0 74-75 32.780708017272644 35.0 32.0 37.0 23.5 39.5 76-77 32.481339033017036 35.0 32.0 36.0 23.0 39.0 78-79 32.167710196502696 35.0 31.5 36.0 23.0 39.0 80-81 31.903428055959402 35.0 31.0 35.5 22.0 37.5 82-83 31.629165289794198 35.0 31.0 35.0 20.5 37.0 84-85 31.40190842273963 35.0 31.0 35.0 20.0 37.0 86-87 31.190568676745478 34.0 31.0 35.0 20.0 36.0 88-89 31.001738672188623 34.0 31.0 35.0 20.0 36.0 90-91 30.779336311304405 34.0 30.0 35.0 19.0 36.0 92-93 30.58163203561166 34.0 30.0 35.0 18.5 35.5 94-95 30.429817880846024 34.0 30.0 35.0 18.0 35.0 96-97 30.248989091450163 34.0 29.0 35.0 18.0 35.0 98-99 30.023405426685805 34.0 29.0 35.0 17.0 35.0 100 28.863622233726296 33.0 26.0 35.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.5143795732909346 1101 2 -0.47332781273906477 1101 3 -0.5256396589449608 1101 4 -0.4734773860291952 1101 5 -0.3163755162368176 1101 6 -0.4278471289593071 1101 7 -0.36974733301160256 1101 8 -0.45357989846513647 1101 9 -0.624570421873635 1101 10-11 -0.6083418101940055 1101 12-13 -0.60767720241153 1101 14-15 -0.7498639276357721 1101 16-17 -0.7881137145944521 1101 18-19 -0.7988360032725907 1101 20-21 -0.8268042117449994 1101 22-23 -0.8586008822303697 1101 24-25 -0.9290763654495962 1101 26-27 -0.9220757258025074 1101 28-29 -0.877165280229832 1101 30-31 -0.8902939508313992 1101 32-33 -0.9182987289444853 1101 34-35 -0.8954580174112863 1101 36-37 -0.9526975239646092 1101 38-39 -0.9916307182716366 1101 40-41 -1.0362312433554237 1101 42-43 -1.060585445206435 1101 44-45 -0.9568352957199693 1101 46-47 -1.0666846625605118 1101 48-49 -1.0871369348621016 1101 50-51 -1.2262928308636916 1101 52-53 -1.0757821676332995 1101 54-55 -0.9913026287963262 1101 56-57 -0.8708109662030665 1101 58-59 -0.8614075100328975 1101 60-61 -0.8596506704182758 1101 62-63 -0.8476062937997995 1101 64-65 -0.9471219529316954 1101 66-67 -0.9753298370458339 1101 68-69 -0.9097273171773068 1101 70-71 -0.9728004380124773 1101 72-73 -0.951393054016215 1101 74-75 -0.9731884014200674 1101 76-77 -1.0136707450828268 1101 78-79 -1.0127334808063395 1101 80-81 -1.055326201149608 1101 82-83 -1.1112382869568336 1101 84-85 -1.2304074309044388 1101 86-87 -1.2574332830817738 1101 88-89 -1.2776320395315288 1101 90-91 -1.2702918264607277 1101 92-93 -1.3776080302231293 1101 94-95 -1.4968695006327657 1101 96-97 -1.4869134100753776 1101 98-99 -1.6067002327321482 1101 100 -1.6636745469826018 1102 1 -0.5043260933125175 1102 2 -0.4675182468917285 1102 3 -0.5149372501067013 1102 4 -0.309516969166971 1102 5 -0.35411999537041794 1102 6 -0.41813402985779646 1102 7 -0.39886303778865084 1102 8 -0.38015859145702535 1102 9 -0.522248716533376 1102 10-11 -0.5823449766443929 1102 12-13 -0.630633779621256 1102 14-15 -0.7654919886632143 1102 16-17 -0.7856179583831846 1102 18-19 -0.7395608706922587 1102 20-21 -0.8417745123894065 1102 22-23 -0.8512450743125939 1102 24-25 -0.8634766620311396 1102 26-27 -0.9322127648883551 1102 28-29 -0.9620990957559741 1102 30-31 -0.9983346406862239 1102 32-33 -1.052741185748836 1102 34-35 -1.000362853718194 1102 36-37 -0.9912100500381982 1102 38-39 -1.0727148137194433 1102 40-41 -1.0664769710836808 1102 42-43 -1.074332794853781 1102 44-45 -1.1418676194531585 1102 46-47 -1.1415648458405911 1102 48-49 -1.1400275748089612 1102 50-51 -1.1537652274273924 1102 52-53 -1.0393845405722217 1102 54-55 -0.8745267742882419 1102 56-57 -0.8692827723265921 1102 58-59 -0.831346438169021 1102 60-61 -0.8363221033606791 1102 62-63 -0.969324406626896 1102 64-65 -0.9684963708214553 1102 66-67 -0.9648415433821071 1102 68-69 -0.951517977474289 1102 70-71 -0.9646850653580188 1102 72-73 -0.9964019088274014 1102 74-75 -0.9840118988608992 1102 76-77 -1.126370144037665 1102 78-79 -1.1326785354757973 1102 80-81 -1.1484862312161965 1102 82-83 -1.1825244343992019 1102 84-85 -1.1748931478414022 1102 86-87 -1.2651774809680845 1102 88-89 -1.3673803787394085 1102 90-91 -1.4271681435758268 1102 92-93 -1.3703139444416301 1102 94-95 -1.3725872992511086 1102 96-97 -1.364474068129546 1102 98-99 -1.46534289927175 1102 100 -1.6152899633468216 1103 1 -0.5814977112479411 1103 2 -0.4799967515971204 1103 3 -0.5632233255020864 1103 4 -0.3913513596692084 1103 5 -0.37887661412941753 1103 6 -0.3932620481022866 1103 7 -0.46047548180686704 1103 8 -0.4477172590756169 1103 9 -0.647960854191254 1103 10-11 -0.6273785003009493 1103 12-13 -0.6678402264312098 1103 14-15 -0.8031350781172506 1103 16-17 -0.8371791241437379 1103 18-19 -0.7323823581191959 1103 20-21 -0.7694676631394586 1103 22-23 -0.802977563791238 1103 24-25 -0.8565987531757102 1103 26-27 -0.8280392517037001 1103 28-29 -0.9387892244141867 1103 30-31 -1.0011250219704166 1103 32-33 -1.013012745594743 1103 34-35 -0.9936104462496402 1103 36-37 -0.862933405208544 1103 38-39 -0.9573294471155194 1103 40-41 -1.0086998100653801 1103 42-43 -1.0472671510380565 1103 44-45 -1.158819132312992 1103 46-47 -1.0609406580538305 1103 48-49 -1.0874263946932317 1103 50-51 -1.1741398795355806 1103 52-53 -1.0726677230227182 1103 54-55 -0.9060161623738523 1103 56-57 -0.8571297605106238 1103 58-59 -0.8549915327713862 1103 60-61 -0.8837367739042392 1103 62-63 -0.7966588464018898 1103 64-65 -0.896969308370835 1103 66-67 -0.9763057921624139 1103 68-69 -0.8793517529319175 1103 70-71 -0.8654210876621278 1103 72-73 -0.9514182876941746 1103 74-75 -0.9264226484285558 1103 76-77 -0.9517131218807151 1103 78-79 -1.0144878239266646 1103 80-81 -1.0316297513522557 1103 82-83 -1.0131516424151137 1103 84-85 -1.0814067398926213 1103 86-87 -1.0842773302759454 1103 88-89 -1.1209840088330942 1103 90-91 -1.2388480216383968 1103 92-93 -1.3246305526240647 1103 94-95 -1.370973379267756 1103 96-97 -1.4361114022065422 1103 98-99 -1.4900423509915015 1103 100 -1.6118624600669236 1104 1 -0.5084207727501706 1104 2 -0.49370176251628806 1104 3 -0.5150980382731838 1104 4 -0.3140184216430342 1104 5 -0.42726132168068176 1104 6 -0.40438499242108605 1104 7 -0.47719931522000536 1104 8 -0.453782699098106 1104 9 -0.4977937643140322 1104 10-11 -0.5406040279108453 1104 12-13 -0.48679301572523315 1104 14-15 -0.6809407647104138 1104 16-17 -0.7563106360516656 1104 18-19 -0.7886571440567849 1104 20-21 -0.748658799460074 1104 22-23 -0.6652021559058809 1104 24-25 -0.7066261201775532 1104 26-27 -0.7282236525234893 1104 28-29 -0.7965593236104951 1104 30-31 -0.7821428718007226 1104 32-33 -0.8440399579630053 1104 34-35 -1.0414202896362639 1104 36-37 -0.9872668797384918 1104 38-39 -0.9909287946419809 1104 40-41 -0.9622284899356046 1104 42-43 -0.9483222733053296 1104 44-45 -0.9201914463130123 1104 46-47 -0.9536602770245395 1104 48-49 -0.9209378221367572 1104 50-51 -0.9665898053992308 1104 52-53 -0.8098198611218876 1104 54-55 -0.7462322343622318 1104 56-57 -0.7345867900915835 1104 58-59 -0.7805161735300388 1104 60-61 -0.7663810179426989 1104 62-63 -0.8229733483555037 1104 64-65 -0.7661110443002315 1104 66-67 -0.7466467185790435 1104 68-69 -0.8745524227868344 1104 70-71 -0.8531302100791507 1104 72-73 -0.805832881463509 1104 74-75 -0.8094623621689543 1104 76-77 -0.8054142826728743 1104 78-79 -0.8545159407984428 1104 80-81 -0.8939839994467249 1104 82-83 -0.9766226007218002 1104 84-85 -0.8868746300176049 1104 86-87 -0.9377824087484257 1104 88-89 -1.0768451147157059 1104 90-91 -1.1195009112871261 1104 92-93 -1.1250721469125722 1104 94-95 -1.0591894603586063 1104 96-97 -1.2302279194247774 1104 98-99 -1.2079756043314838 1104 100 -1.1577246463257325 1105 1 -0.5678342956754179 1105 2 -0.5233524982361963 1105 3 -0.5780951542428525 1105 4 -0.37405660023677 1105 5 -0.29823895425515445 1105 6 -0.3211924825147179 1105 7 -0.4132093172741307 1105 8 -0.4644197358830269 1105 9 -0.6200857700850761 1105 10-11 -0.6250447946375459 1105 12-13 -0.6748770975017635 1105 14-15 -0.8087684102172545 1105 16-17 -0.8143392877055717 1105 18-19 -0.8473764768488294 1105 20-21 -0.8108635825221739 1105 22-23 -0.7944278359609385 1105 24-25 -0.8681588179573296 1105 26-27 -0.9061516661984612 1105 28-29 -0.9154290228416144 1105 30-31 -0.9502992886927615 1105 32-33 -1.0047460783158684 1105 34-35 -0.962847726290633 1105 36-37 -0.9280347336781816 1105 38-39 -0.9689264227794823 1105 40-41 -0.9041158860288974 1105 42-43 -0.9510636257142053 1105 44-45 -1.0611358881575228 1105 46-47 -1.132823293336351 1105 48-49 -1.1127154212285078 1105 50-51 -1.0790264621290788 1105 52-53 -0.9885842792281423 1105 54-55 -0.8892814641814439 1105 56-57 -0.7890837836464186 1105 58-59 -0.7345978093370107 1105 60-61 -0.7519932744952484 1105 62-63 -0.8011810434835382 1105 64-65 -0.7959776104896434 1105 66-67 -0.7992816720754092 1105 68-69 -0.914715635056929 1105 70-71 -0.783355951914352 1105 72-73 -0.726474721389998 1105 74-75 -0.8724579418169434 1105 76-77 -0.8128465251596353 1105 78-79 -1.0034243742444993 1105 80-81 -0.9257895581385576 1105 82-83 -1.1078483352481072 1105 84-85 -1.0142600876001957 1105 86-87 -1.0827706401607244 1105 88-89 -1.2112100089689584 1105 90-91 -1.2515430871535216 1105 92-93 -1.1812291496795098 1105 94-95 -1.3355894666711592 1105 96-97 -1.3700709640747206 1105 98-99 -1.2775071145800574 1105 100 -1.2209790279969823 1106 1 -0.558480037681381 1106 2 -0.5778360135288629 1106 3 -0.6330366502349101 1106 4 -0.43115089711083954 1106 5 -0.4858122616519793 1106 6 -0.45347298393209456 1106 7 -0.45061187554036053 1106 8 -0.41318448161564447 1106 9 -0.5934995442570994 1106 10-11 -0.5619720072869541 1106 12-13 -0.698223536228646 1106 14-15 -0.8471934592149211 1106 16-17 -0.8335198428011594 1106 18-19 -0.9172782751127428 1106 20-21 -0.7777281531670823 1106 22-23 -0.7312542363047498 1106 24-25 -0.8356102188975782 1106 26-27 -0.839915074305793 1106 28-29 -0.9130254945363916 1106 30-31 -0.9327176013890082 1106 32-33 -0.892445791213305 1106 34-35 -0.9138649983571767 1106 36-37 -1.0090102012877011 1106 38-39 -0.9909154696271827 1106 40-41 -1.0461578621204524 1106 42-43 -0.9746913298017361 1106 44-45 -1.1121144859056287 1106 46-47 -1.074927022156352 1106 48-49 -1.1196061707323466 1106 50-51 -1.130811107675342 1106 52-53 -0.9727903336781978 1106 54-55 -0.7983816794067522 1106 56-57 -0.8515255596579223 1106 58-59 -0.946017304918847 1106 60-61 -0.839703373472453 1106 62-63 -0.8488449495890009 1106 64-65 -0.9169005881916092 1106 66-67 -0.9507908770898297 1106 68-69 -0.9246969104236982 1106 70-71 -0.9090565704878415 1106 72-73 -0.9041205040778593 1106 74-75 -0.8679357910851486 1106 76-77 -0.8770300858032591 1106 78-79 -0.8329330753829609 1106 80-81 -0.9082624432600319 1106 82-83 -0.9007463438034655 1106 84-85 -0.9443433384710858 1106 86-87 -0.8734682295051357 1106 88-89 -0.8962436927734281 1106 90-91 -0.9982280685711515 1106 92-93 -1.0042427779056453 1106 94-95 -1.0654920488835096 1106 96-97 -1.0936993874296448 1106 98-99 -1.0813008400730553 1106 100 -1.2751562979668734 1107 1 -0.6315729957615943 1107 2 -0.5503073640015366 1107 3 -0.534951072332781 1107 4 -0.4420999057377344 1107 5 -0.3726022127757105 1107 6 -0.37020952804635954 1107 7 -0.3785401997528979 1107 8 -0.417524630249261 1107 9 -0.5489366871081316 1107 10-11 -0.6056490903895053 1107 12-13 -0.6475935721661514 1107 14-15 -0.8208925096495392 1107 16-17 -0.8306616926230745 1107 18-19 -0.8238619469933326 1107 20-21 -0.7478167434643481 1107 22-23 -0.8480687994445191 1107 24-25 -0.8760342049971612 1107 26-27 -0.8608302157102088 1107 28-29 -0.8884212564803633 1107 30-31 -0.860272887752636 1107 32-33 -0.9145370705723153 1107 34-35 -0.9555608755781648 1107 36-37 -0.9764719621939548 1107 38-39 -1.0308076859473374 1107 40-41 -0.9503258721927068 1107 42-43 -0.9701091730756914 1107 44-45 -0.9244400432121296 1107 46-47 -0.815623392045147 1107 48-49 -0.9255973027795648 1107 50-51 -0.9587864000503359 1107 52-53 -0.8116889257733035 1107 54-55 -0.7438204324784579 1107 56-57 -0.7637964217105662 1107 58-59 -0.7643681614181546 1107 60-61 -0.7483416543620436 1107 62-63 -0.6797688101381851 1107 64-65 -0.6913733978377081 1107 66-67 -0.6506662359670017 1107 68-69 -0.7053280253223733 1107 70-71 -0.7146552154868075 1107 72-73 -0.6344607817734058 1107 74-75 -0.6528459418911652 1107 76-77 -0.6145520121673194 1107 78-79 -0.5716344731230407 1107 80-81 -0.7228408675719074 1107 82-83 -0.6803224096136518 1107 84-85 -0.7495526771674328 1107 86-87 -0.7881940673822427 1107 88-89 -0.682488433558941 1107 90-91 -0.7995358113610287 1107 92-93 -0.920722529821731 1107 94-95 -0.9212741406307359 1107 96-97 -0.8470292633914198 1107 98-99 -0.7907127783902688 1107 100 -0.8280310626637934 1108 1 -0.5765750327480035 1108 2 -0.49319811394601487 1108 3 -0.5724369485542553 1108 4 -0.40316565287206885 1108 5 -0.4551811208119716 1108 6 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58-59 1.1257218880530857 2311 60-61 1.0844199437100528 2311 62-63 1.0885704109829888 2311 64-65 1.1225560553330212 2311 66-67 1.1471099934642197 2311 68-69 1.1094178648812587 2311 70-71 1.1292205650159346 2311 72-73 1.1398946774963505 2311 74-75 1.2134564799425647 2311 76-77 1.1860079648057322 2311 78-79 1.2009141287309006 2311 80-81 1.158374846018166 2311 82-83 1.2364130097186354 2311 84-85 1.2731967245735412 2311 86-87 1.2253912097910344 2311 88-89 1.2824621016062814 2311 90-91 1.365377226659426 2311 92-93 1.3626438853731528 2311 94-95 1.3670774179098686 2311 96-97 1.4195139189428545 2311 98-99 1.574552867466263 2311 100 1.6792578630976607 2312 1 0.6748958740685111 2312 2 0.6505206686075908 2312 3 0.6780251963535378 2312 4 0.38246825773239834 2312 5 0.5085965835061401 2312 6 0.5641857592743804 2312 7 0.5051839522464263 2312 8 0.5951507088995172 2312 9 0.8023062732626514 2312 10-11 0.751553147179834 2312 12-13 0.8791727008813908 2312 14-15 1.0573350810763813 2312 16-17 1.08420738881928 2312 18-19 1.0479192739505265 2312 20-21 1.09066843357229 2312 22-23 1.1431461929617441 2312 24-25 1.140635402105353 2312 26-27 1.194807906415015 2312 28-29 1.2385627185068273 2312 30-31 1.3005269163969828 2312 32-33 1.259246467400125 2312 34-35 1.2209405255599464 2312 36-37 1.277723982851846 2312 38-39 1.2737568374770873 2312 40-41 1.34265787292005 2312 42-43 1.419858074498464 2312 44-45 1.4109720433367556 2312 46-47 1.44477985116702 2312 48-49 1.4023079570658368 2312 50-51 1.4062979036727228 2312 52-53 1.3004036508409982 2312 54-55 1.2219678740942967 2312 56-57 1.2041244741675001 2312 58-59 1.1323642525128932 2312 60-61 1.1011109674649333 2312 62-63 1.0928733880451915 2312 64-65 1.0932819700470233 2312 66-67 1.0933888471025313 2312 68-69 1.1100345591932452 2312 70-71 1.1305074862130056 2312 72-73 1.1501458396787427 2312 74-75 1.1777619741054153 2312 76-77 1.1215232365186552 2312 78-79 1.0945461152200409 2312 80-81 1.0599266069468065 2312 82-83 1.1443493322810845 2312 84-85 1.1793981685298185 2312 86-87 1.1537199079755922 2312 88-89 1.1523809023203704 2312 90-91 1.1731558257970995 2312 92-93 1.2278646097995605 2312 94-95 1.3077167940335421 2312 96-97 1.3063899412243103 2312 98-99 1.0344921314831446 2312 100 1.3631409494463043 2313 1 0.5993097930493505 2313 2 0.5843824200590646 2313 3 0.629872671326666 2313 4 0.47872298730772656 2313 5 0.2855971209010306 2313 6 0.48854834822642346 2313 7 0.5690292875111567 2313 8 0.5507701333490118 2313 9 0.7078526937581557 2313 10-11 0.7256746689590443 2313 12-13 0.8269933261215172 2313 14-15 0.9634576575889753 2313 16-17 0.9511382600581229 2313 18-19 0.9306658906218672 2313 20-21 0.9116826198192598 2313 22-23 0.9436967086423351 2313 24-25 0.9092196824455598 2313 26-27 0.9476169579451152 2313 28-29 1.0226953312066414 2313 30-31 1.0979012182872836 2313 32-33 1.0667760722410051 2313 34-35 1.0601023840372221 2313 36-37 1.0803094604739556 2313 38-39 1.0965709455085744 2313 40-41 1.139573861367026 2313 42-43 1.175306606744563 2313 44-45 1.127672309709986 2313 46-47 1.190734128450309 2313 48-49 1.2068048052270797 2313 50-51 1.2319238955840248 2313 52-53 1.1481386953727153 2313 54-55 1.0347863956557504 2313 56-57 0.914916980647348 2313 58-59 0.9460536178507439 2313 60-61 0.924169538123742 2313 62-63 0.9370327677397441 2313 64-65 0.9918965202974874 2313 66-67 0.9205100504471204 2313 68-69 0.9275919102927546 2313 70-71 0.9391288244559561 2313 72-73 0.953751866183076 2313 74-75 0.8983567998500703 2313 76-77 0.7071274450246463 2313 78-79 0.9341816491324266 2313 80-81 1.0111095061143978 2313 82-83 1.034463372888066 2313 84-85 1.1028931386581213 2313 86-87 1.0904778856334758 2313 88-89 1.102515215147914 2313 90-91 1.1350108784554749 2313 92-93 1.1812586486692211 2313 94-95 1.2679469080333199 2313 96-97 1.3421453837441248 2313 98-99 1.312026681774885 2313 100 1.3883562652320975 2314 1 0.5741163281997359 2314 2 0.5691608033890461 2314 3 0.6121327929525648 2314 4 0.471765874734416 2314 5 0.5024393387104951 2314 6 0.5046232855918618 2314 7 0.5322439179288381 2314 8 0.5148055080972114 2314 9 0.643281397732018 2314 10-11 0.7041017041929649 2314 12-13 0.751366548406672 2314 14-15 0.898238688091709 2314 16-17 0.8874975918339842 2314 18-19 0.8485641404157676 2314 20-21 0.8925290293186379 2314 22-23 0.9596227960654389 2314 24-25 0.9251177061043663 2314 26-27 0.9987390352978522 2314 28-29 1.0050843968262413 2314 30-31 1.028351683865786 2314 32-33 1.0261598451181086 2314 34-35 1.0608182735363485 2314 36-37 1.0696864528118155 2314 38-39 1.1004161862773358 2314 40-41 1.1680266480691728 2314 42-43 1.2130870541666 2314 44-45 1.2276727412068311 2314 46-47 1.2646882355437796 2314 48-49 1.1707543911559029 2314 50-51 1.2236944327642263 2314 52-53 1.0837750009331728 2314 54-55 1.016078059393621 2314 56-57 1.0069025119542516 2314 58-59 1.0084634385784028 2314 60-61 0.9467211827514674 2314 62-63 0.965846666795521 2314 64-65 1.0026204411277178 2314 66-67 1.0329662532428188 2314 68-69 1.028959077853692 2314 70-71 1.05928486886706 2314 72-73 1.0831884634968887 2314 74-75 1.0747838537931713 2314 76-77 1.0534928175367497 2314 78-79 1.0622604861753047 2314 80-81 1.055782281195338 2314 82-83 1.1455051883141003 2314 84-85 1.2447139589452654 2314 86-87 1.259325988445056 2314 88-89 1.244734291651998 2314 90-91 1.2825252776589195 2314 92-93 1.3313504894240076 2314 94-95 1.3300093902697547 2314 96-97 1.3693757552545627 2314 98-99 1.4184072772321876 2314 100 1.5420330432642544 2315 1 0.4431287256751766 2315 2 0.47875389063754525 2315 3 0.5598924031326362 2315 4 0.2401858505454868 2315 5 0.32341708696431226 2315 6 0.3941734206510503 2315 7 0.41238458408271583 2315 8 0.4760738213723812 2315 9 0.5736313214617752 2315 10-11 0.48079162745655424 2315 12-13 0.5176111065442797 2315 14-15 0.7154595441831049 2315 16-17 0.7341048609449317 2315 18-19 0.5679092328335429 2315 20-21 0.7654888003266223 2315 22-23 0.5988019391971022 2315 24-25 0.735860447482203 2315 26-27 0.8438160760124376 2315 28-29 0.8576841891225584 2315 30-31 0.8376009075668023 2315 32-33 0.8768949983190026 2315 34-35 0.903692250233135 2315 36-37 0.9651655309389682 2315 38-39 1.001025636017964 2315 40-41 1.0262324003216747 2315 42-43 1.0515617658547214 2315 44-45 1.094969867540911 2315 46-47 1.086131018617742 2315 48-49 1.0275253823064858 2315 50-51 0.9474495055770547 2315 52-53 0.9985120887307133 2315 54-55 0.8787218592314971 2315 56-57 0.875574121295216 2315 58-59 0.8483709042567895 2315 60-61 0.8303573633345351 2315 62-63 0.810138376517969 2315 64-65 0.8052174493659408 2315 66-67 0.8219271391689489 2315 68-69 0.8662935882608949 2315 70-71 0.8653075634727188 2315 72-73 0.6087383149706298 2315 74-75 0.7967552518047043 2315 76-77 0.8155375269199325 2315 78-79 0.9275658455518823 2315 80-81 0.599551623924544 2315 82-83 0.6335166780571164 2315 84-85 0.8715795692237442 2315 86-87 1.0225889174813645 2315 88-89 0.9947824623315924 2315 90-91 1.0775239762736213 2315 92-93 1.223537580717803 2315 94-95 1.2040714540467015 2315 96-97 1.0387497889274186 2315 98-99 0.5463788384024753 2315 100 1.007726066544162 2316 1 0.3925226743522785 2316 2 0.3447739153493252 2316 3 0.36746957639081046 2316 4 0.25797271654023746 2316 5 0.21087534754084913 2316 6 0.2656055296279618 2316 7 0.24991330386950494 2316 8 0.27756980315509594 2316 9 0.3724031357353823 2316 10-11 0.37305898441925933 2316 12-13 0.13718638557296714 2316 14-15 0.10436043156069275 2316 16-17 0.3189676186571475 2316 18-19 0.3745446523721938 2316 20-21 0.4184064753769121 2316 22-23 0.3887694156341084 2316 24-25 0.4831523630536836 2316 26-27 0.5241980364123222 2316 28-29 0.4629048081424614 2316 30-31 0.46584420117747527 2316 32-33 0.6031424582134122 2316 34-35 0.5482275768473173 2316 36-37 0.569784183227874 2316 38-39 0.7307449990258661 2316 40-41 0.8399161661111734 2316 42-43 0.765315990854468 2316 44-45 0.7176602861094636 2316 46-47 0.5553768497811404 2316 48-49 0.486493614392991 2316 50-51 0.5496108929086958 2316 52-53 0.6887908876564595 2316 54-55 0.43529664810915847 2316 56-57 0.2401922374814376 2316 58-59 0.3609582124086188 2316 60-61 0.38272936243593136 2316 62-63 0.5658054924897442 2316 64-65 0.5015484646933714 2316 66-67 0.4454859164463656 2316 68-69 0.42984358366572195 2316 70-71 0.2239985538841367 2316 72-73 0.14149252975889226 2316 74-75 0.3820339996730624 2316 76-77 0.5542039331938682 2316 78-79 0.5565161564914405 2316 80-81 0.7082612502865011 2316 82-83 0.7396457358438759 2316 84-85 0.750964378626481 2316 86-87 0.774743156777177 2316 88-89 0.8053057751398534 2316 90-91 0.7211772863247816 2316 92-93 0.7903086597648681 2316 94-95 0.9300848157679482 2316 96-97 0.8717963073234003 2316 98-99 0.8112883646919293 2316 100 0.6088115468411104 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 35.0 1 515.0 2 207.0 3 164.0 4 147.0 5 125.0 6 134.0 7 456.0 8 1553.0 9 3212.0 10 5016.0 11 7711.0 12 10838.0 13 13996.0 14 17366.0 15 20546.0 16 25728.0 17 32146.0 18 40923.0 19 50063.0 20 61971.0 21 78357.0 22 86719.0 23 86968.0 24 83417.0 25 84983.0 26 92201.0 27 106531.0 28 125640.0 29 149370.0 30 183114.0 31 227451.0 32 290259.0 33 384418.0 34 559631.0 35 775393.0 36 1165481.0 37 1599398.0 38 1312294.0 39 379981.0 40 317.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.020066936105 20.369582780825944 12.729695849643882 22.88065443342518 2 15.726870441694665 19.761322973238077 41.420092923002045 23.091713662065214 3 19.306816069298797 26.889174927552283 28.36696327021315 25.437045732935765 4 12.38704913596666 16.227545934984253 39.289414397934785 32.0959905311143 5 12.98060985321123 38.55043500616813 34.11066969655667 14.358285444063965 6 29.567598730550053 37.697492425717066 18.341817299400446 14.393091544332428 7 27.582021096287875 32.151634516285235 22.70195212436187 17.56439226306502 8 26.784203435416597 34.83570987279084 19.761944933095474 18.618141758697092 9 27.155881408721722 15.288392787587721 21.49221304988342 36.06351275380714 10-11 26.12462271514106 26.094206720595103 26.79069826291427 20.990472301349566 12-13 26.108544598469663 23.886936963730125 27.625610865189977 22.37890757261024 14-15 23.472097159627193 24.92640469779651 25.742025374713922 25.859472767862375 16-17 21.825628942995277 27.629130140183193 26.93341988216816 23.61182103465337 18-19 21.225807283741073 27.54600674614307 29.539775034309883 21.688410935805976 20-21 22.852828264212263 26.66776592638821 28.954947549312276 21.524458260087247 22-23 23.15547395277238 26.387680898555043 28.62222374364406 21.834621405028518 24-25 22.43465270275801 26.298245374682644 29.514368033497508 21.75273388906184 26-27 22.321348677611716 26.644580612495787 28.531025221819117 22.503045488073376 28-29 21.566911719794238 27.16574990070651 29.060657237634974 22.206681141864273 30-31 22.636174577388815 26.72286600372722 28.974266501855734 21.66669291702823 32-33 22.814965116102403 26.917346200630238 28.154395160252605 22.11329352301475 34-35 22.766961358864602 26.79343745695907 28.922401152599285 21.517200031577044 36-37 22.315321673604785 26.51688952387461 28.57701771594033 22.59077108658028 38-39 22.63416947031952 26.703781089726853 28.93747396283241 21.724575477121213 40-41 22.356330635069035 25.714455455073814 29.503651003867926 22.425562905989224 42-43 22.25736390839884 26.026227576476423 28.762454067631054 22.953954447493683 44-45 22.22211333421822 26.938517284454473 28.65916001037055 22.180209370956756 46-47 22.246774266981486 26.624775877475425 28.05177870756311 23.07667114797998 48-49 22.960520154240147 26.550901791301946 28.048075688569867 22.440502365888044 50-51 22.732842324055458 26.475306920075447 27.939094376008455 22.85275637986064 52-53 22.211490804945814 27.04808378560241 27.681397976463508 23.059027432988273 54-55 22.073389628183207 26.914554038596567 27.808816899544325 23.2032394336759 56-57 23.03713477509698 26.485435659982475 27.262116937464587 23.215312627455962 58-59 22.082519377549055 26.93995526098945 28.126850880405797 22.850674481055698 60-61 22.333394912196788 27.521287031733205 27.427889321511927 22.717428734558077 62-63 22.639645956738313 26.81756282890553 27.1543573775333 23.38843383682286 64-65 21.882615151871263 26.87052393020052 27.558032127704635 23.68882879022358 66-67 22.36587608358257 26.529437940235702 27.461560048808696 23.643125927373028 68-69 21.900371089814204 26.68755435928349 27.71574685213115 23.696327698771157 70-71 22.02125594971651 26.297156199716092 27.88733638337743 23.794251467189966 72-73 22.593474350396473 26.552653266847194 27.87007427061856 22.983798112137773 74-75 22.80822412231601 26.7731849794965 27.443136307405396 22.975454590782096 76-77 22.489050244388636 26.583645863841475 27.271956080800148 23.655347810969744 78-79 22.29940575001814 26.64730894611939 27.323667495338334 23.729617808524136 80-81 21.981497124987303 27.084298936826368 27.7497855259504 23.184418412235928 82-83 22.93169864158778 26.17316066173142 28.64816094078077 22.246979755900036 84-85 22.997617356035313 26.613648198333994 27.461884579235097 22.926849866395592 86-87 21.856408822290174 27.30741196265542 27.836794018517274 22.999385196537133 88-89 22.762787752926634 26.081419579554478 27.80141835175905 23.35437431575984 90-91 22.685770046555838 26.083417276385507 27.937180004578938 23.293632672479717 92-93 22.981762173614396 26.334329413689233 27.778909475872478 22.9049989368239 94-95 21.86823533468154 26.372126132507333 28.327301417936795 23.43233711487433 96-97 22.204415931226954 26.259109316260133 27.93763027206727 23.598844480445646 98-99 21.66181909739707 26.74307841527276 27.719796439300165 23.875306048030005 100 22.459685441511578 26.257933792984378 26.978071400539893 24.30430936496415 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1907.0 1 1373.0 2 1721.5 3 2378.0 4 1663.0 5 1352.0 6 2005.0 7 3457.0 8 5403.0 9 6433.5 10 5723.5 11 4879.0 12 5160.0 13 6005.5 14 7002.5 15 8183.0 16 9205.5 17 10164.5 18 11200.5 19 12128.0 20 12944.0 21 13608.0 22 14652.5 23 16208.0 24 17859.5 25 19636.5 26 22782.5 27 26712.5 28 32005.5 29 43841.0 30 62509.0 31 81585.5 32 107833.0 33 138806.5 34 164781.5 35 188945.5 36 211853.0 37 247393.5 38 262919.5 39 250730.5 40 234202.0 41 215362.5 42 210091.0 43 221576.5 44 243123.0 45 282263.0 46 319708.5 47 345623.0 48 365602.5 49 364902.5 50 354979.5 51 362847.0 52 361094.0 53 341682.0 54 321785.5 55 279515.0 56 243947.0 57 202638.0 58 161437.0 59 149171.0 60 122930.0 61 87431.5 62 65853.0 63 54106.0 64 39886.0 65 21829.0 66 12929.5 67 8378.0 68 5922.5 69 4420.0 70 3383.5 71 2573.5 72 2105.5 73 1922.5 74 1525.5 75 1106.0 76 812.5 77 541.0 78 366.5 79 267.5 80 206.5 81 161.5 82 125.0 83 99.0 84 68.5 85 50.5 86 46.5 87 40.0 88 32.0 89 23.0 90 15.5 91 12.5 92 11.0 93 10.5 94 11.5 95 8.5 96 3.5 97 3.5 98 4.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05643058857810664 2 0.1338537032961242 3 0.017037053110595148 4 0.016280677390255772 5 0.019293780669640506 6 0.053020698035593054 7 0.058079735640485936 8 0.05003239396015388 9 0.041402270987101315 10-11 0.04001351556615033 12-13 0.041668862429516014 14-15 0.0197215669377013 16-17 0.018655201168042507 18-19 0.01861180256113779 20-21 0.021984494297733043 22-23 0.026553747624701245 24-25 0.025853170113239368 26-27 0.020056356190966268 28-29 0.01931238007259967 30-31 0.017285045150050683 32-33 0.02427222086171034 34-35 0.024247421657764785 36-37 0.02419782324987368 38-39 0.022096090715488032 40-41 0.02555557966589272 42-43 0.032858945227858184 44-45 0.04321261287512671 46-47 0.048284050081992366 48-49 0.04116667854961856 50-51 0.028940671004460756 52-53 0.04085048869931275 54-55 0.03714300770945253 56-57 0.039269539447783726 58-59 0.04435957605760855 60-61 0.024098626434091466 62-63 0.026590946430619575 64-65 0.026132161157626836 66-67 0.033323930301837314 68-69 0.0402925066105378 70-71 0.041681262031488794 72-73 0.045215148593730145 74-75 0.027886704836774738 76-77 0.02102352514484285 78-79 0.020707335294537044 80-81 0.025146392800791097 82-83 0.020893329324128695 84-85 0.012945184459578847 86-87 0.015158513411719483 88-89 0.01912638604300802 90-91 0.021934895889841936 92-93 0.020713535095523435 94-95 0.024408616483410882 96-97 0.025809771506334647 98-99 0.028705078566978 100 0.030093833987928986 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8064775.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.05268585836644 #Duplication Level Percentage of deduplicated Percentage of total 1 79.86355453066352 53.55065833477099 2 12.822494887539015 17.19565443229327 3 3.7631645705807117 7.569908753534489 4 1.4850262384588637 3.9829999143525514 5 0.7133713404120822 2.391673219450657 6 0.3824603916856346 1.5386997898178787 7 0.22784831259324373 1.0694488929371555 8 0.15051857219431294 0.8074139629756094 9 0.10079131605418314 0.6082495607369223 >10 0.441848036156245 5.11873351210922 >50 0.028414685377258147 1.3190197465486961 >100 0.018468243197711783 2.333777561411823 >500 0.0015060104010905012 0.7046432534551211 >1k 4.401925670145318E-4 0.5681537375426082 >5k 2.316802977654905E-5 0.09567874836373051 >10k+ 6.950408932964714E-5 1.145286579699248 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32536 0.4034334497862619 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25295 0.31364793190138596 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15893 0.19706687415333968 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.2399601972776674E-5 0.0 5 0.0 0.0 0.0 1.2399601972776674E-5 0.0 6 0.0 0.0 0.0 1.2399601972776674E-5 2.4799203945553348E-5 7 0.0 0.0 0.0 1.2399601972776674E-5 2.4799203945553348E-5 8 0.0 0.0 0.0 2.4799203945553348E-5 2.4799203945553348E-5 9 0.0 0.0 0.0 3.719880591833002E-5 3.719880591833002E-5 10-11 0.0 0.0 0.0 3.719880591833002E-5 4.3398606904718355E-5 12-13 0.0 0.0 0.0 3.719880591833002E-5 4.9598407891106696E-5 14-15 0.0 0.0 0.0 4.9598407891106696E-5 6.199800986388336E-5 16-17 1.2399601972776674E-5 0.0 0.0 8.059741282304838E-5 6.81978108502717E-5 18-19 1.2399601972776674E-5 0.0 0.0 1.1159641775499006E-4 7.439761183666004E-5 20-21 1.2399601972776674E-5 0.0 0.0 1.9219383057803843E-4 7.439761183666004E-5 22-23 1.2399601972776674E-5 1.2399601972776674E-5 0.0 4.835844769382903E-4 8.679721380943671E-5 24-25 1.2399601972776674E-5 1.2399601972776674E-5 0.0 0.0012027613913593374 9.919681578221339E-5 26-27 1.2399601972776674E-5 1.2399601972776674E-5 0.0 0.0023807235787731214 1.0539661676860172E-4 28-29 1.2399601972776674E-5 1.2399601972776674E-5 0.0 0.0060448059617286285 1.1159641775499006E-4 30-31 1.2399601972776674E-5 1.2399601972776674E-5 0.0 0.020601938677768442 1.1159641775499006E-4 32-33 1.2399601972776674E-5 1.2399601972776674E-5 0.0 0.053845271566782704 1.363956217005434E-4 34-35 1.2399601972776674E-5 1.2399601972776674E-5 0.0 0.1029600949809511 1.363956217005434E-4 36-37 1.2399601972776674E-5 1.2399601972776674E-5 0.0 0.1832909163615848 1.487952236733201E-4 38-39 1.2399601972776674E-5 1.2399601972776674E-5 0.0 0.304565223456327 1.6119482564609677E-4 40-41 1.2399601972776674E-5 1.2399601972776674E-5 0.0 0.4353872240701073 1.6119482564609677E-4 42-43 1.2399601972776674E-5 1.2399601972776674E-5 0.0 0.5620305588190619 1.7359442761887342E-4 44-45 1.2399601972776674E-5 1.2399601972776674E-5 0.0 0.7069385072739165 1.7359442761887342E-4 46-47 1.2399601972776674E-5 1.2399601972776674E-5 0.0 0.8748291179853127 1.7359442761887342E-4 48-49 1.2399601972776674E-5 1.2399601972776674E-5 0.0 1.0615733234963156 1.7979422860526178E-4 50-51 1.2399601972776674E-5 1.2399601972776674E-5 0.0 1.2573568388454732 1.859940295916501E-4 52-53 1.2399601972776674E-5 1.2399601972776674E-5 0.0 1.442916882368076 1.859940295916501E-4 54-55 1.2399601972776674E-5 3.099900493194169E-5 0.0 1.647919501783993 1.859940295916501E-4 56-57 1.2399601972776674E-5 3.719880591833002E-5 0.0 1.878986084546686 1.9839363156442678E-4 58-59 1.2399601972776674E-5 4.9598407891106696E-5 0.0 2.1474560666602605 2.1079323353720346E-4 60-61 1.2399601972776674E-5 4.9598407891106696E-5 0.0 2.418808956232505 2.1079323353720346E-4 62-63 1.2399601972776674E-5 4.9598407891106696E-5 0.0 2.696969227287804 2.1079323353720346E-4 64-65 1.2399601972776674E-5 4.9598407891106696E-5 0.0 2.974726511278988 2.1079323353720346E-4 66-67 1.2399601972776674E-5 6.199800986388336E-5 0.0 3.279192537919533 2.1079323353720346E-4 68-69 1.2399601972776674E-5 6.199800986388336E-5 0.0 3.614762966108788 2.1079323353720346E-4 70-71 1.2399601972776674E-5 6.81978108502717E-5 0.0 3.977550520628288 2.1079323353720346E-4 72-73 1.2399601972776674E-5 7.439761183666004E-5 0.0 4.342408808677241 2.6039164142831016E-4 74-75 1.2399601972776674E-5 7.439761183666004E-5 0.0 4.687700524813153 2.6039164142831016E-4 76-77 1.2399601972776674E-5 7.439761183666004E-5 0.0 5.069267276520424 2.6039164142831016E-4 78-79 1.2399601972776674E-5 7.439761183666004E-5 0.0 5.486011699024461 2.6039164142831016E-4 80-81 1.859940295916501E-5 7.439761183666004E-5 0.0 5.92301087135103 2.6039164142831016E-4 82-83 2.4799203945553348E-5 8.059741282304838E-5 0.0 6.367877591129325 2.6039164142831016E-4 84-85 2.4799203945553348E-5 8.679721380943671E-5 0.0 6.799600732816477 2.6039164142831016E-4 86-87 2.4799203945553348E-5 8.679721380943671E-5 0.0 7.252793785319492 2.6039164142831016E-4 88 2.4799203945553348E-5 8.679721380943671E-5 0.0 7.610020118354201 2.727912434010868E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16750 0.0 48.26758 1 GTATCAA 30165 0.0 40.967415 1 TACCTGG 5425 0.0 36.78082 2 GTACTAG 7120 0.0 36.21994 1 CAACGCA 34840 0.0 33.933594 5 ACCTGGG 5685 0.0 33.818745 3 ATCAACG 35025 0.0 33.76736 3 TATCAAC 37705 0.0 32.60089 2 GCATCGT 7460 0.0 32.577797 8 GGGCACC 8075 0.0 31.427765 94 AACGCAG 39670 0.0 30.548939 6 GTGGTAT 5435 0.0 30.131926 1 TAGGCAT 9110 0.0 29.928137 5 TCAACGC 41420 0.0 28.99631 4 TCTAACG 3810 0.0 28.7797 2 CTGTGCG 9885 0.0 28.390326 9 GTACATG 42475 0.0 28.207994 1 GTACCTG 7130 0.0 28.116886 1 ACGCAGA 42310 0.0 27.887625 7 TAGTACT 10360 0.0 27.768578 4 >>END_MODULE